Scintigraphy를 이용한 척수 손상 환자와 정상인의 담즙 배설양상 비교

신용일,양충용,주민철,위훈,정환정,김창근,길은영 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.1

외상성 뇌손상과 출혈성 뇌졸중 환자의 간질발생에 대한 보고

위훈,길은영,양충용,주민철,신용일 최신의학사 2004 最新醫學 Vol.47 No.1

고전압 응용을 위한 4H-SiC Schottky Barrier Diode의 제작 및 특성

금병훈,강수창,박종대,신무환 명지대학교 대학원 1998 대학원논문집 Vol.2 No.-

This paper discusses about the processing and characterization of the high breakdown voltage 4H-SiC Schottky Barrier Diode. Two types of the devices were fabricated one was fabricated using the passivation layer and the other was fabricated without the passivation. In case of the device with passivation layer, breakdown voltages were in the range 120 V to 300 V, which is quite higher compare to the devices fabaricated from Si or GaAs. However, this value is lower than the theoretically predicted value. The reverse leakage current of the devices was quite low which is less than 10^7 Amp at the reverse bias voltage of 220 V.

혈청이 마우스 간 세포주 BNL CL.2의 Nitric Oxide 생성에 미치는 영향

유지창,정헌택,김유현,진효상,김신무,배현옥 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1999 Journal of biomedical laboratory sciences Vol.5 No.1

마우스 간 세포주인 BNL CL.2의 시험관내 배양에서 혈청과 IFN-γ가 세포주의 nitric oxide(NO) 생성과 세포 손상에 미치는 영향을 알아보기 위한 실험을 하였다. 혈청이 공급된 배양에서 IFN-γ에 의한 세포 생존율은 거의 변동이 없었으나, 혈청을 제거한 배양에서는 약 65%의 생존율이 유지되었으며, NO 생성 억제제인 N -monomethy-L-arginine (NMA)의 첨가는 농도 의존적으로 세포의 생존율을 감소시켰다. 혈청이 제거된 BNL CL.2세포주는 IFN-γ단독 처리에서도 NO 를 생성할 수 있었으며, IFN-γ와 lipopolysaccharide (LPS)의 복합 처리는 세포주의 NO 생성을 상승적으로 증가시켰다. 또한 protein tyrosine kinase (PTK) inhibitor인 herbimycin A와 genistein에 의해서 NO 생성이 억제되어 PTK의 활성이 혈청이 고갈된 BNL CL.2 세포에서 NO의 생성에 중요한 역할을 담당하고 있기 때문으로 판단된다. IFN-γ의 독성은 혈청을 제거시킬 때 NO 생성 억제제에 상승적으로 간 세포를 손상시키며, 이때 NO가 IFN-γ에 의해 유도된 손상을 어느 정도 억제시키는 것을 알 수 있었다. Nitric oxide (NO) plays an important role in immunologic defense, and influences upon the functioning of secretory tissues and cells. It also exhibits cytotoxic/cytostatic activity as one of major operating effectors of the cellular immunity system. We investigated the effects of serum on the cell damages and NO production in the mouse liver cell line BNL CL.2 to establish the role of NO. We observed that, when BNL CL.2 cells were cultured in serum-free medium, they were induced to cell damage by the stimulation of IFN-γalone or IFN-γplus LPS. Serumstarved cells showed large amount of nitrite accumulation and NO synthase (NOS) expression in response to IFN-γalone in dose-and time-dependent manners, but serum-supplied cells did not. The production of NO was blocked by protein tyrosine kinase (PTK) inhibitors, genistein and herbimycin. These results suggest that the deprivation of serum in the BNL CL.2 cell culture medium might primed with the cells to produce NO when the cells are triggered by IFN-γ and the involvement of PTK signal transduction pathway in the expression of NOS gene in murine hepatocytes.

Synthesis and Characterization of Pr(Ⅲ)-,Sm(Ⅲ)-,Gd(Ⅲ)-,Dy(Ⅲ)-[18]HAM Complexes

정덕상,박신복,한충훈,변종철 濟州大學校 基礎科學硏究所 1998 基礎科學硏究 Vol.11 No.1

Lanthanide(III) and mercury(II) complexes of the 18-membered hexaaza Schiff base macrocycle 2,11,17,26,31,32-hexaazapentacyclo[26,2.1.1??,O??,O??] dotriaconta-1(31), 2,4,6,8,10,12,14,16(32) ,17,19,21,23,25,27,29-hexadecane([18]HAM) ahve been synthesized by the metal template condensationof phthalic dicarboxaldehyde and 2,6-diaminopyridine. Discrete mononuclear complexes [Pr([18]HAM)(NO₃)(OH)]NO₃·2H₂O,[Sm([18]HAM)(NO₃)(H₂O)(OH)]NO₃·2H₂O, [Gd([18]HAM)(NO₃)(H₂O)(OH)]NO₃·3H₂O, [Dy([18])HAM)(NO₃)₂(H₂O)₂]NO₃·2H₂O, and [Hg([18])HAM)(H₂O)₂(ClO₄)]ClO₄· ?H₂O, have been synthesized by using the respective hydrated lanthanide(Ⅲ) nitrate and mercury(Ⅱ) perchlorate as the template. The lanthanide(Ⅲ)-marcrocyclic complexes (lanthanide(Ⅲ)-[18]HAM) was decomposed by c-HClO? forming [18] HAM and liberating the lanthanide(Ⅲ) ion. Co(Ⅱ)- Ni(Ⅱ)-Cu(Ⅱ)-complexes of acyclic, potentially tridentate compartmental Schiff bases ([9]acy) complexes containing d-metal ion Co(Ⅱ), Ni(Ⅱ), and Cu(Ⅱ) ion have been prepared by using metal template condensation of phthalic dicarboxaldehytde and 2,6-diaminopyridine. The complexes and macrocycle were characterized by elemental analysis, fast atom bombardment mass spectrometry, infrared spectroscopy, and molar conductivity. The template potential of the lanthanide(Ⅲ) cations of various sites in the assembly of [18]HAM is due to the flexibility of the macrocycle to adapt to the geometrical requirements of the metal ions and to the steric demands of the exocyclic ligands

비만세포주에서 Histamine 유리에 관한 연구

김형룡,국윤아,신민,이홍수,원진숙,김형민,이영미,정헌택,이혜숙,안녕형 원광대학교 생명공학연구소 1995 생명공학연구소보 Vol.3 No.1

발달장애유아의 언어행동발달 연구

김춘희,변찬석,서경희,유은정,이효신,전헌선,최성규 大邱大學校 特殊敎育·再活科學硏究所 2000 特殊敎育硏究 Vol.23 No.1

본 논문은 발달장애유아 언어행동발달 컨텐츠 개발에 요구되는 기초연구로써 연구의 이론적 바탕을 확립하고자 하였다. 연구에 사용된 방법은 문헌조사 방법 및 현장 인터뷰기법을 병행하였다. 본 연구의 주된 목적은 크게 3가지로 구분된다. 첫째, 발달장애유아의 가정 내 교육 욕구를 분석하고자 하였다. 둘째, 발달장애유아의 언어발달 양상을 검토하고자 하였다. 세 번째 목적은 유아의 언어행동발달 컨텐츠 구성의 주요 지침을 설정하고자 하였다. 이상의 연구목적에 따른 연구결과를 요약하면 다음 몇 가지로 제시된다. 1.가정 내 부모-유아간 관계형성이 우선 고려 되어야하고, 이에 따른 상호작용은 발달적 애착형성이 가능한 방향으로 정립되어야한다. 2.발달장애유아의 언어행동발달양상을 검토한 결과에 따르면, Piaget는 언어행동발달에 있어 감각운동기적 환경탐색 기능을 강조하고 있고, 이는 현재까지 중요한 유아 발달의 기저로 정착되었다. 따라서, 발달장애유아의 언어행동발달을 위한 컨텐츠 개발 내용에 있어서도 이러한 기저에 충실함이 합리적이다. 3.발달장애유아들에게는 언어행동 양상의 갈래가 크게 운동장애 및 감각결함으로 나누어질 수 있으므로, 이 두 가지 갈래의 각기 다른 특성이 컨텐츠 구성에서 충분히 고려되어야한다. 4.자폐성과 같은 중증 전반적 발달장애 유아를 위한 언어행동발달 컨텐츠 구성에서 특히 고려해야 할 두 가지 요소는 모방행동기능과 반복단순훈련기능 향상으로 결론지을 수 있다. This article planed to establish the theoretical foundations of the research as a basic study needed for developing the contents of verbal(language) behavior development for the infants with developmental disabilities. The methods used in the study was previous literature researches and interviews with subjects. The purposes of the study were divided into three parts. First, it was to analyze the educational needs of the infants with developmental disabilities in home settings. Second, it was to review and discover the language patterns of the infants with developmental disabilities. The third purpose was to devise the major guidelines of their language development's contents. The results of the study were described as the followings. 1.The relationships between the infants with developmental disabilities and their parents and their interactions should move to build the developmental attachments between them. 2.According to the literature findings of their language development patterns, Piaget concentrated on the functions of sensory motor environmental exploring in the language behavior development. The functions have been settled down into the important foundation for the infants' development. Thus, it was resonable that the content's development of the language behaviors for the infants with developmental disabilities should based on the function addressed by Piaget. 3.The language behaviors' patterns of the infants with developmental disabilities generally were classified into motor disabilities and sensory defect(deficiency). Therefore, the two factors should be considered for the constructions of the contents. 4.Constructing the contents of the language behavior developments for the infants with severely pervasive developmental disabilities such as autism, the improvements of two functions, imitative behavior and repetitive simple training should be considered.

Effect of Platelet-Rich Fibrin on Repair of Defect in the Articular Disc in Rabbit Temporomandibular Joint by Platelet-Rich Fibrin

( Hyun Su Baek ),( Hye Sung Lee ),( Bok Joo Kim ),( In Kyo Chung ),( Chul Hoon Kim ),( Sun Mi Jin ),( Hie Sung Hwang ),( Sang Hun Shin2 ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.6

The objective of this study was to evaluate platelet-rich fibrin (PRF)’s effectiveness in repairing articular disc defect in the temporomandibular joint (TMJ) of rabbits. Eight rabbits were divided into four groups of two rabbits each, corresponding to groups A, B, C, and D. Both TMJs of all of the rabbits were used in the experiments: the right joints comprised the experimental groups, and the left ones, the control groups. The disc defect was circular and 2 mm in diameter. In the experimental groups, the PRF was compressed into the defect, whereas the control group defects were left untreated. A, B, C, and D groups were sacrificed at the 1st, 2nd, 4th and 6th weeks, respectively. The defects of each control group exhibited no specific changes. Contrastingly, in each experimental group, there was an increased number of chondroblasts at the margins of the defects, along with accelerated cell differentiation and a columnar cell arrangement observable at the time of cell differentiation. The experimental groups showed inflammatory cell infiltrations and fibrosis by the 1st week, maturation of chondrocytes by the 2nd week, and proliferation by the 4th week, after which the defects began to be filled with chondrocytes, a process that was complete after the 6th week. In the histological evaluation (H-E), the experimental groups showed significant increases of chondroblasts after the 2nd and 4th weeks, as well as regular columns of chondrocyte arrays observable during cell division. After 6 weeks, the defects were filled with chondrocytes.

Studies on the Optimal Culture Conditions on the Hemolysin Production by Vibrio vulnificus

Chung Yun-Shin(정윤신),Park Moon-Kook(박문국),Jahng Kwang-Yeop(장광엽),Chung Hun-Taeg(정헌택) 大韓微生物學會 1988 大韓微生物學會誌 Vol.23 No.4

93년도 대전지역에서 유행한 홍역 질환의 임상적 및 혈청학적 고찰

정진기,이재호,박하신,유재홍,문신혜,정재원,정용헌,길홍량,이건수,한지환,최혜진,황성수,유연덕 대한감염학회 1994 감염 Vol.26 No.2

1993년 11월부터 1994년 1월까지 대전지역 4개 종합병원에서 홍역으로 진단된 환아 133예를 대상으로 홍역 예방접종 유무와 시기에 따라 15개월에 예방접종을 받았던 환아군은 GroupⅠ, 15개월 이전에 예방접종을 받았던 환아군은 GroupⅡ, 6-9개월과 15개월에 2회 예방접종을 받았던 군은 GroupⅢ, 예방접종을 받지 않았던 군은 GroupⅣ로 분류하여 각군에서 발병연령, 임상증상, 합병증, 홍역에 대한 IgM과 IgG 특이 항체 등을 비교 분석하여 다음과 같은 결과를 얻었다. 1) 대상환아 133예중 예방접종을 받았던 환아는 71예(53.4%)이고 예방접종을 받지 않았던 환아는 62예(46.6%)이었다. 연령에 따른 빈도는 1세 이하가 39.1%, 5-6세가 12.0%, 1-2세가 11.2%이었다. 각 군에서 많이 발생하였던 환아의 연령분포는 GroupⅠ은 6-8세 사이가 64.7%, GroupⅡ는 2세이하가 70%, GroupⅢ은 6세 이상이 80%, GroupⅣ는 1세이하가 80.6%이었다. 2) 모든 환아에서 발진과 발열이 나타났고, 출현 시기에 따른 발진과 발열증상의 상호 관계에서 발열은 환아의 60예(45.1%)에서 발진 출현 2일전에 발생하였으며 발열은 43예)32.3%)에서 발진과 함께 발생하였다. 전체 환아의 46예(34.6%)에서 발열기간이 3일 이하이었다. 3) 전체 환아에서 기침증상이 있었고, 전체 환아의 80예(60.2%)에서 10일 이상 기침이 지속되었다. 예방접종을 받지 않았던 군에서 62예중 42예(67.7%)에서 기침이 10일 이상 지속되었으며 이중 24예(57.1%)에서 합병증이 있었다. 예방접종을 받았던 군에서는 71예중 38예(53.5%)에서 기침이 10일 이상 지속되었으며 이중 17예(44.7%)에서 합병증이 있었다. 4) 합병증은 88예(66.1%)에서 관찰되었으며 예방 접종을 받지 않았던 GroupⅣ(71.0%)에서 가장 많이 발생하였다. 합병증으로는 폐렴(46.6%), 중이염(7.5%), 급성위장염(6.8%), 간염(6.8%) 등이 있었다. 5) 예방접종을 받았던 환아 71예중 64예에서 혈청검사를 실시한 결과 55예에서 홍역에 대한 IgM 특이항체가 양성으로 나타나 일차 면역 실패(primary vaccine failure)는 전체 환아의 85.9%에서 있었고, IgM은 9예에서 음성으로 나타나 이차 면역 실패(secondary vaccine failure)는 전체 환아의 14.1%에서 나타났다. 예방접종을 받지 않았던 환아 62예중 혈청검사를 시행한 53예에서 IgG 음성이 20예(37.7%) 이었으며 IgG가 음성으로 나타난 연령은 6개월에서 14개월로 고루 분포하였다. 결론적으로 홍역 유행시 예방접종을 받았던 환아에서 발생한 홍역질환의 주원인은 일차 백신 실패이었으며 예방접종을 받지 않았던 환아에서 모체로 부터의 수동 항체가 생후 6개월부터 소실되는 소견이 관찰되므로써 홍역 이환의 위험이 높은 경우에는 조기에 예방접종을 시행하여야 할 것으로 사료된다. A clinical study was made on 133 case of measles patients during epidemics in Taejeon from Nov. 1993 to Jan. 1994. The patients were divided into four groups by vaccination history. We evaluated the clinical manifestations, complications, and serological antibody of measles (IgM & IgG) in each group. The results were summerized as follows. 1) Out of 133 patients, 71 patients(53.4%) were the vaccinated group and 62 patients (46.6%) were the unvaccinated group. Peak ages of measles in each group were 5-8 year old(64.7%) in GroupⅠ, less than 2 year old (70%) in GroupⅡ, more than 6 year old (80%) in GroupⅢ, and less than 1 year old(80.6%) in GroupⅣ. 2) Fever developed before 2 days of rash appearence(45.1%) and at the same time(32.3%), Duration of fever was less than 3 days in 46cases(34.6%). 3) All patients had cough and 80 cases(60.2%) had cough more than 10 days. Cough continued more than 10 days in 42 cases(67.7%) of 62 unvaccinated cased and 38 cases(53.5%) of 71 vaccinated cases. 4) Complications such as pneumonia, otitis media, gastroenteritis, and hepatitis were noted in 66.1% of all patients and most common in GroupⅣ. 5) In the 64 patients of vaccinated group carried out serologic test, measles specific IgM antibodies were positive in 85.9% (primary vaccine failure) and negative in 14.1%(secondary vaccine failure). In the 53 patients of unvaccinated group carried out serologic test, measles specific IgG antibodies were negative in 37.7%, less than 14 month old. In conclusion, primary vaccine faliure (85.9%) was the main cause of measles during epidemics in the vaccinated group. maternal measles IgG antibodies were not detected in 37.7%, less than 14 month old.