알데히드탈수소효소 2(ALDH2) 및 CYP2E1 유전자 다형성에 따른 요중 뮤콘산 농도와 요중 8-hydroxydeoxyguanosine과의 관련성

김용대,강종원,엄상용,장연위,김성훈,김은영,이철호,문재동,김헌 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.2

Quantitative comparison of mRNA expression of glucosyltransferase(GTF) between xylitol-resistant(X^(R)) and xylitol-sensitive(X^(s)) mutans streptococci

Kim, Chong-Chul,Lee, Mi-Na,Kim, Young-Jae,Lee, Sung-Hoon 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Since the long-term exposure of mutans streptococci to xylitol is known to select for xylitol-resistant(X^(R)) natural mutants, the occurrence and survival of such X^(R) strains were performed in batch culture methods. The aim of the study was to compare the differentiation and quantification of mRNA expression of the gtf genes of X^(R) and X^(S) mutans streptococci. Using a real-time reverse-transcription polymerase chain reaction, the expression of each gtf was determined. In X^(R) strains, the relative levels of transcription of gtfB and gtfC were decreased while that of gtfD was increased, suggesting the presence of independent promoters. It also suggested that mutation related to production of glucosyltransferase occurred under the exposure of xylitol could explain the caries-preventive mechanisms of xylitol. 자일리톨에 장기간 노출된 mutans streptococci는 자일리톨에 내성이 발현되어 자일리톨 내성균주가 생성된다고 알려져 있다. 본 연구의 목적은 mutans streptococci에서 자일리톨 내성균주와 감성균주의 gtf 유전자 발현량을 각각의 유전자별로 정량적으로 분석하고 비교하는 것이다. 실시간 역전사 중합효소연쇄반응법을 이용하여 각각의 gtf 발현을 조사한 결과 gtfD는 증가한 반면. gtfB와 gtfC는 감소하였는데 이는 각 유전자의 독립된 조절기전이 존재함을 보여주는 것이다. 또한 자일리톨에 노출된 mutans streptococci에서의 glucosyltransferase와 연관된 유전자변형이 자일리톨의 치아우식증 예방효과의 작용기전 중 하나임을 알 수 있었다.

Wiskott-Aldrich 증후군 환아의 증례보고

이연주,현홍근,장철호,김영재,김정욱,장기택,김종철,한세현,이상훈 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.3

Apoptotic Effect of Co-treatment with Chios Gum Mastic and HS-1200 on G361 Human Melanoma Cell Line

Young-Joo Hur,Young-Ki Kim,Hyun-Ho Kwak,Gyoo-Cheon Kim,Seung-Eun Lee,In-Ryoung Kim,Chul-Hoon Kim1,Bong-Soo Park 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.2

Chios gum mastic (CGM) is a resinous exudate obtained from the stem and the main leaves of Pistacia lenticulus tree native to Mediterranean areas. Recently it reported that CGM induce apoptosis in a few cancer cells in vitro. Bile acids and their synthetic derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. It has been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis-inducing activity on various cancer cells in vitro. This study was undertaken to investigate the synergistic apoptotic effect of cotreatment with a natural product, CGM and a CDCA derivative, HS-1200 on G361 human melanoma cells. To investigate whether the co-treatment of CGM and HS-1200 compared with each single treatment efficiently reduced the viability of G361 cells, MTT assay was conducted. To investigate augmentation of apoptosis in G631 cells co-treated with CGM and HS-1200, DNA electrophoresis, Hoechst staining, proteasome activity assay, flow cytometry, Westen blot analyses, immunofluorescent staining and confocal microscopy were performed. In this study, G361 cells co-treated with CGM and HS-1200 showed several lines of apoptotic manifestation such as nuclear condensations, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA content, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, activation of caspase-9, caspase-3, PARP and DFF45 (ICAD), and up-regulation of Bax whereas each single treated G361 cells did not. Although the single treatment of 40 μg/mL CGM or 25 μM HS-1200 for 24 hrs did not induce apoptosis, the co-treatment of them induced prominently apoptosis. Therefore, combination therapy of CGM and HS-1200 could be considered, in the future, as an alternative therapeutic strategy for human melanoma.

음낭외상시 고환파열에 대한 초음파 검사의 진단적 유용성

김경영,한동준,신대은,노준,문형윤,임동훈,김철성,박성운 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.3

Purpose: We determined the use of scrotal ultrasonography in the initial diagnosis and management of testicular injuries due to blunt scrotal trauma. Mateiials and Methods: We reviewed 54 patients of scrotal trauma in the last 8 years. Forty patients were evaluated by ultrasonography preoperatively. A heterogeneous echo pattern of the testicular parenchyma with loss of oval shape, contour definition was the basis for diagnosis of testicular rupture. Among them, 33 cases underwent scrotal exploration and 7 cases were managed conservatively. The remaining 14 patients were evaluated only by physical examination, 8 patients of them were explored immediately because of suspicious testicular mpture. The accuracy of ultrasonography were determined by comparing radiographic interpretations with intraoperative findings. Results: Among the 33 patients who were evaluated by ultrasonography and explored immediately, there were 26 suspected testicular ruptures on ultrasonography. Surgical exploration revealed testicular rupture in 23 and simple hematoma in 10. The testicular rupture was correctly diagnosed by ultrasonography in the 22 cases, and there were 4 false-positive and 1 false-negative diagnoses of testicular rupture. The sensivity and specificity for the diagnosis of testicular rupture are 96% and 60%, and the positive and negative predictive values are 85% and 86%, respectively, Conclusions: Scrotal ultrasonography, with the main radiographic finding of a heterogeneous echo pattern of the testicular parenchyma with loss of oval shape, contour definition, is highly sensitive on the diagnosis of testicular rupture. And it increases the salvage rate for testicular rupture with preservation of the testicular function and prevents delayed complications of chronic pain, atrophy and orchiectomy associated with missed testicular rupture.

중학교 응급처치 교육의 실태 조사 연구

김훈철,김세환,박기동,김승재 강원대학교 체육과학연구소 2000 江原大學校附設體育科學硏究所論文集 Vol.- No.23

The purpose of this study was to investigate the current status of first aids program in the safety education that was a part of the physical education curriculum in middle school. And this study is to supply the fundamental data for efficient safety education. The data was gathered with questionnaires given to 145 physical education teachers in Kangwon Province. Teachers think that the first aids education is essential in middle school curriculum. Taking considerations of the numerous accidents in society. This need will provide a remedy for any emergent situation and enlighten the safety-first-mind to protect against accidents. However, it is difficult to determine that efficient and good quality of first aids education is implemented in middle school. Based on the data, followings are some findings from the result; a) teachers lack experiences with first aids education, b) teachers are not equipped with sufficient theoretical and practical knowledge about first aids, and c) classroom teaching materials for first aids education are not furnished in the school at all. To suggest based on the above conclusions, the information about first aids must be employed in depth during the training period for present teachers along with learning physical education teachers so as to develop the theoretical knowledge and applicable capability about the education. Schools must stock adequate teaching materials for first aids education, and seek for effective and high quality education.

CO_2 레이저빔을 이용한 TFT-LCD 도광판의 패턴 제작에 관한 연구

김경동,백창일,송철기,안성훈 경상대학교 생산기술연구소 2002 工學硏究院論文集 Vol.2 No.-

A light guide panel is an element of the LCD backlight module that is often used for the display of compact electronic devices. In this study, a laser marking system is proposed to fabricate light guide panel, which can replace other manufacturing methods such as silk printing, stamping, or v-cutting methods. The objective of this research is to evaluate the process parameters of the laser marking system. Light guide patterns were marked with a 50W CO_2 laser(CW) to understand the effects of average power and scanning speed on the geometry and quality of groove pattern. The width of the fabricated grooves increases with increasing laser power and decreasing scan speed. In order to analyze surface characteristics and optical properties(luminance, uniformity), SEM photography and BM7(luminance measuring system) were used. As a result, the optimal conditions of the process parameters that provide high luminance was obtained.

眉叟 許穆 散文의 特徵과 그 意味

김철범,신승훈 경성대학교 한국학연구소 2006 文化傳統論集 Vol.13 No.-

사이버 공간의 공동체와 분화 : 대학생PC통신 사용자를 중심으로

김철규,나은영,마동훈 한국사회문화학회 2000 사회와 문화 Vol.12 No.-

Community has been an important topic for social scientists, especially in regards to the processes of modernization and urbanization. Recently, in the context of the so-called "information revolution." on-line communities have gartered increasing interest. Despite wide and casual usage of the term "on-line community" in mass-media, little work has been done to conceptualize on-line communites and how they mimic and/or diverge from their more conventional (off-line) counterparts. This study hopes to fill this intellectual gap by re-examining the concept of community in the existing literature and by interpreting primary data collected through a survey of on-line interaction among college students. The existing literature indicates that communities in the 'real world' are not as spatially bound and homogeneous as the ideal-typical notion implies. Furthermore, our primary data shows that space and homogeneity even less significant for on-line communities, where interaction and subjective definition by participants are primary in community formation. Spatial locations(such as rural and urban)and off-line variables(such as gender or income)are not significant in the formation and types of on-line communities. This leads us to cautiously predict that on-line interaction may help construct a civil society based on solidarity other than traditional networks(such as school and regional ties).

분자역학적 방법을 이용한 부산시내 대형건물 냉각탑수의 레지오넬라균의 형별 분류

김정만,정석훈,서대영,박은희,송은주,최재철,이은엽,장철훈 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1