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김창근,김대응,김우형,김명현 대한핵의학회 2013 핵의학 분자영상 Vol.47 No.2
Purpose The purpose of this study was to estimate lean body mass (LBM) using CT (LBM CTs) and compare the results with LBM estimates of four different predictive equations (LBM PEs) to assess whether LBM CTs and LBM PEs can be used interchangeably for SUV normalization. Methods Whole-body F-18 FDG PET/CT studies were conducted on 392 patients. LBM CT1 is modified adipose tissue-free body mass, and LBM CT2 is adipose tissue-free body mass. Four different PEswere used for comparison (LBM PE1–4). Agreement between the two measurement methods was assessed by Bland-Altman analysis. We calculated the difference between two methods (bias), the percentage of difference,and the limits of agreement, expressed as a percentage. Results For LBM CTs vs. LBM PEs, except LBM PE3, the ranges of biases and limits of agreement were −3.77 to 3.81 kg and 26.60–35.05 %, respectively, indicating the wide limits of agreement and differing magnitudes of bias. For LBM CTs vs. LBM PE3, LBM PE3 had wider limits of agreement and greater positive bias (44.28–46.19 % and 10.49 to 14.04 kg, respectively), showing unacceptably large discrepancies between LBM CTs and LBM PE3. Conclusion This study demonstrated that there are substantial discrepancies between individual LBM CTs and LBM PEs,and this should be taken into account when LBM CTs and LBM PEs are used interchangeably between patients.
CD-ROM을 이용(利用)한 서지정보(書誌情報) 데이터베이스의 신뢰성향상(信賴性向上) 기법연구(技法硏究) - 다운로드와 업로드를 중심(中心)으로 -
김창근,신용주,Kim, Chang-Keun,Shin, Yong-Ju 한국과학기술정보연구원 과학기술정보센터 1989 Journal of Information Science Theory and Practice Vol.20 No.2
본연구(本硏究)는 레이저기술(技術)과 정보공학(情報工學)의 눈부신 발전과정(發展過程)에서 등장한 대용량 정보축적매체(情報蓄積媒體)인 CD-ROM Catalog Production Product 중에서 BiblioFile을 선택하고, CD-ROM BiblioFile과 과학기술대학도서관(料學技術大學圖書館)에서 자체 개발(開發)한 시스템인 KITLAS를 시스템구성(構成), 파일구조(構造), 특성(特性) 및 기능면(機能面)에서 비교분석(比較分析)한 후 CD-ROM에 수록(收錄)된 MARC 레코드의 변환(變換)과 온라인 대량, 동시 전송(傳送)하는 과정(過程)인 이른바 다운로드(download)와 업로드(up-load)하는 과정(過程)을 구현하여 서지정보(書誌情報) 데이터베이스의 신뢰성(信賴性) 향상(向上) 방법(方法)을 모색하였다. With regard to system configuration, file structure and function, this study has compared and analyzed KITLAS developed by KIT Library and CD-ROM BiblioFile, which is selected among CD-ROM Catalog Production Products, the mass storage media, produced by the fast-growing laser technology and information technology. Furthurmore, this study illuminates how to improve the reliability of bibliographic database, implementing downloading and uploading processes which make possible the conversion and online, mass transmission of CD-ROM MARC records at once.
김창근,정영채,신언익,임홍순,김홍률,정영호,윤종택,이종완,권처진 韓國受精卵移植學會 1995 한국동물생명공학회지 Vol.10 No.2
This study was conducted to examine the efficiency of enucleation and blastomere isolation from recipient oocytes and donor embryos, respectively and to determine the effect of oocyte age and electric voltage on the fusion rate and in vitro development of the fused oocytes in rabbit nuclear transplantation. Immature oocytes collected from ovarian follicles were matured in vivo for 12 h in TCM-199 containing FCS and hormones and in vivo matured oocytes were collected 17 to 18 h post-HCG. The fresh and frozen donor embryos of 8- to 16-cell stage were collected from the oviduct of superovulated does. The proportion of successfully enucleated oocytes was greatly lower in in vitro matured oocytes (42.3%) than that (62.7%) in in vivo matured oocytes The level of cytochalasin B for in vivo matured oocytes did not affect the efficiency of enuleation, but 7.5 g /mL cytochalasin B for in vitro matured oocytes showed a high enucleation rate significantly. The isolation efficiency of a single blastomere nucleus did not differ between 8- and 16-cell stage embryos. The percentage of single blastomeres isolated from 16-cell stage fresh embryos after 0.5% pronase treatment was greatly higher at 16-min treatment (94.4%) than at 8-min(78. 1%) and the blastomeres(61.5%) isolated from frozen-thawed embryos after 16-min pronase were significantly fewer than those of fresh embryos. The age of recipient oocytes affected nuclear fusion rate. The reconstituted oocytes fused at 24-h age showed slightly higher fusion rate (77.8%) than those (65.0%)fused at 18-h age. The fusion rate of in vitro and in vivo matured oocytes inserted with fresh blastomere did not differ among electric voltages, but the cleavage rate and development to morula-blastocysts of in vitro matured oocytes was more higher under 0.6 kV/cm than under 0.8 to 1.2 kV/cm, while the cleavage rate and development of in vivo matured oocytes was higher under 0.8 to 1.0 kV/cm than under 1.2 kV/cm. The fusion and cleavage rate fol1owing insertion with frozen-thawed blastomere was not different between the in vitro and in vivo matured oocytes and was similar to those from fresh blastomere insertion.
소 미성숙 난포란의 체외성숙시 β-Mercaptoethanol의 첨가가 체외성숙, 체외수정 및 Glutathione 수준에 미치는 영향
김창근,오신애,정영채,방명걸 한국발생생물학회 2006 발생과 생식 Vol.10 No.4
본 연구는 소의 미성숙 난포란의 체외성숙시 β-mercaptoethanol(β-ME)의 첨가가 체외성숙, 체외수정 후 웅성전핵의 형성 및 세포질 내의 GSH 수준에 미치는 영향을 알아보고자 실시하였다. 체외성숙시 25μM과 50μM의 β-ME를 첨가한 경우 대조구에 비하여 성숙율이 증가하는 것으로 나타났으며(p<0.05), 모든 실험구에 있어서 12시간 체외성숙보다 24시간 체외성숙에서 높은 성숙율을 나타냈다(p<0.05). 체외수정 후 웅성전핵 형성에 있어서는 25μM와 50μM 농도의 β-ME 첨가구에서 대조구에 비하여 높게 나타났으나(p<0.05), 25μM과 50μM 농도구와의 유의적인 차이는 없었다. GSH의 수준은 체외성숙 후 50μM의 β-ME 첨가구가 다른 처리구에 비교하여 높게 나타났으며(p<0.05), 체외수정 후 웅성전핵이 형성된 다음 세포질 내 GSH 수준 역시 50μM의 β-ME 첨가구에서 가장 높은 결과를 나타냈다(p<0.05).