소의 시상하부내에서 Vasopressin과 Oxytion분비세포의 분포에 관한 면역조직화학적 연구

유명철,김종중,장인엽,문정석,정주현,김흥중,조사선 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

This study was done to observe the distributions of Oxytocinergic and Vasopressinergic neurons in the hypothalamus of the cow. For the immunohistochemical staining, sections were reacted with the following sequence of solutions: monoclonal anti-vasopressin and anti-oxytocin, biotinylated anti-mouse IgG, avidin-biotin peroxidase complex, and Ni-DAB. The results observed under light microscope were summarized as follows. Our immunohistochemical investigations reveal both vasopressinergic and oxytocinergic neurons in the supraoptic nucleus(SON), paraventricular nucleus(PVN), accessory supraoptic nucleus (ASN), periventricular nucleus(PN), suprachiasmatic nucleus(SCN), and arcuate nucleus(AN), but these two types of neurons were mainly distributed in the PVN and SON and the ratio of the number of vasopressinergic neurons to that of oxytocinergic neurons was 1:1.30 in the PVN, and 1:1.00 in the SON. These two types of neurons were round, oval and spindle-shaped and cell size was 30-55um.

연산 증폭기 (TL494) 를 이용한 DC/DC 승압 컨버터 제어 시스템 설계

정구종,이혜연,문경주,정유석,이준영,손영익 明知大學校 産業技術硏究所 2009 産業技術硏究所論文集 Vol.28 No.-

A simple control system using an op Amp (TL494) is presented for a DC/DC boost converter. The DC/DC boost converters have many industrial applications including renewable energy sources and hybrid automobiles. The problem of regulating the output voltage in the presence of load variation has been a research subject of interest for many years. After computer simulations with SimPower system of Matlab Simulink, we have developed the converter system in university laboratory according to the design specifications. Since the chip TL494 has both op Amp and PWM comparator even beginners can easily develop the control system. Experimental results show that the proposed system has some robustness with respect to the load variation.

Prevotella nigrescens의 용혈특성에 관한 연구

곽주석,장훈상,장석우,이수종,유용욱,민경산 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.4

세균의 용혈활성은 세균이 숙주 내에서 생존하기 위해 필요한 철을 획득하기위한 특성이며 기능면에서 볼 때 숙주에 대한 중요한 독력인자로 간주될 수 있다. 본 연구에서는 괴사치수 및 치근단 치주염으로 진단된 환자의 근관에서 분리한 Prevotella nigrescens의 용혈활성을 다양한 조건 하에서 측정하여 그 특성을 규명하는 것을 목적으로 한다. 1. 임상에서 분리한 P. nigrescers와 표준균주인 P. nigrescers ATCC 33563에서 모두 용혈활성이 나타났다. 2. 사람, 면양 및 말 세 가지 종에 대해 용혈활성을 비교한 결과 사람의 적혈구에서 가장 강한 용혈활성을 나타내었다. 3. 용혈소 억제제인 NaN₃와 dithiothreitol (DTT)는 농도의존적으로 P.nigrescers의 용혈활성을 감소시켰다 (p<0.05). 4. P. nigrescers가 최대 용혈활성을 나타내는 최적의 pH는 4이었으며, 50℃이하의 온도에서는 용혈활성을 보였으나 95℃에서 급격히 감소하였다. 5.배양조건에 따른 P. nigrescens의 용혈활성을비교한 결과 10% CO₂배양기에 배양한 경우혐기성 조건에서 배양한 것보다 더 높은 용혈활성을 보였다. Hemolytic property is a specific feature of bacteria to obtain iron which is essential for its survival in host tissues. Therefore, it is thought to be one of several factors of virulence. The purpose of this study was to investigate the hemolytic properties of Prevotella nigrescens isolated from the teeth diagnosed as pulp necrosis and apical periodontitis under the presence of hemolysin inhibitors such as NaN3 and dithiothreitol, heat, various pH and cultural conditions. The results were as follows; 1. Clinically isolated P. nigrescens strains and standard P. nigrscens ATCC 33563 showed hemolytic activity. 2. P. nigrescens showed higher hemolytic activity against human erythrocytes than sheep or horse erythrocytes. 3. NaN₃ and dithiothreitol (DTT) reduced the hemolytic activity of P. nigrescens in a dose dependent manner (p<0.05). 4. Optimal pH for the maximum hemolytic activity of P. nigrescens was 4.0 and the hemolysin was stable under the 50℃, but the hemolytic activity was significantly decreased at 95℃. 5. P. nigrescens cultured in 10% CO₂ condition showed higher hemolytic activity than the bacteria cultured in the anaerobic condition.[J Kor Acad Cons Dent 30(4):335-343,2005]

흡연습관의 변화가 혈청지질변화에 미치는 영향

신용하,이종영,김진석,유재영 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Objectives : This study was conducted to assess the effect of smoking status change on the lipid profile levels and to construct fundamental data on smoking and health promotion. Methods : 581 male examinees who had taken two times of general health screening tests for aged people from 1996 till 2000 were classified into four groups - continuous non-smoker, continuous smoker, smoking stopper and new smoker and change of serum lipid profile levels between two times of tests among 4 groups were compared. Results : At the first tests, 344(59.2%) out of 581 examinees were smokers and among them, 30(5.2%) stopped smoking while among initial non-smokers, 28(4.8%) began smoking. Low-density lipoprotein cholesterol levels according to smoking status change were +6.8±27.4 for continuous non-smokers, +11.2±25.0 for continuous smokers, +10.8±28.7 for smoking stoppers, and -7.6±22.5 for new smokers (adjested by alcohol intake and obesity index, p=0.002). Differences of triglyceride levels by smoking status changes were -3.9±78.5 for continuous non-smokers, -13.6±89.6 for continuous smokers,+5.5±65.0 for smoking stoppers, +31.4±73.7 (adjusted by alcohol intake and obesity index, p=0.048) Conclusions: Although the results of this study should be interpreted in the restricted sense since there had not been effective controls over important confounding variables that might have been related to serum lipid profile levels, like changes of health belief or behavior, For smoking cessation to be more effective strategy for prevention of hyperlipidemia and atherosclerotic cardiovascular diseases, elevation of lipid profile levels and weight gain following smoking cessation should also be prevented through concomitant dietary modification and sufficient exercise.

Expansin 유전자를 이용한 담배의 형질전환과 형질전환체 선발

백승준, 김보미, 장종오, 엄유리, 석영선, 이이 충북대학교 연초연구소 2008 煙草硏究 Vol.21 No.-

-본문참고-

유해화학물질의 계통분석

박성우,박유신,이진훈,유재훈,김유나,오세진,이진숙,김남이,박종신,남금문,노동석 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

항정신병약물 사용 중인 정신분열병 환자에서 올란자판으로의 교체 방법에 관한 연구(II) : Comparison of Safety 안전성 비교

안용민,권용실,권준수,민성호,박두병,양문정,소형석,송종호,신윤식,우행원,유범희,이홍석,정한용,한창환,김용식 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.5

연구목적: 이 다기관 공동임상연구는 사용 중인 항정신병약물을 ’직접 교체 방법’또는 ’시작-감량 교체 방법’중 한 가지 방법으로 올란자핀으로 교체한 후, 안정성 측면에서 두 교체 방법 간의 비교와 교체후의 변화를 관찰하기 위한 것이다. 방법: 국내 13개 병원의 입원 및 외래에 내원한 환자들 중 ICD-10 지단기준으로 정신분열병에 해당되며, 임상적으로 항정신병약물 교체가 필요한 환자를 대상으로 하였다. 두 가지 교체 방법 중 한 가지를 무작위로 피험자에 적용하였으며, ’직접 교체 방법’에 배정된 경우에는 사용중인 항정신병약물을 일시에 중단하고 10㎎의 올란자핀을 바로 투여하였고, ’시작-감량 교체 방법’에 배정된 경우는 10㎎의 올란자핀 투여하고 2주에 걸쳐서 기존 약물을 감량하여 중단하였다. 올란자핀 사용기간은 총 6주이며, 용량은 5∼20㎎ 범위로 제한하였다. 한정성 평가를 위해서 체중, 생명징후, 자발적인 이상반응 복, 실험실 검사 그리고 Simpson-Angus Scale(SAS), Barnes akathisia rating scale(BARS), Abnormal involuntary movement scale(AIMS). Liverpool University neuroleptic side effect rating scale(LUNSERS)등을 이용하였다. 결과: 총 103명의 정신분열병 환자를 대상으로 하였다. 사용한 올란자핀의 용량, 벤조디아제핀의 병용률, 탈락률과 탈락 사유, 자발적인 이상반응 보고, 생명징후, 실험실 검사 그리고 대부분의 부작용 척도 상에서 임상적으로 의미 있는 차이를 두 교체 방법간에 발견하지 못하였다. 다만 AIMS의 감소는 ’직접 교체 방법’군에서 보다 적었고, 항콜린제의 병용률은 ’시작-감량 교체 방법’군에서 보다 많았다. 기저 상태에서 전체 피험자의 SAS와 BARS 점수는 각각 3.5점과 1.8점이었으며 70% 이상의 피험자가 고프로락틴 혈증을 보였다. 올란자핀으로 교체한 후, SAS, BARS, AIMS 점수의 유의한 감소가 있었으며 고프로락틴 혈증을 보인 피험자 분율도 약 30%이하로 감소하였다. 그러나 교체 방법과 상관없이 올란자핀 교체 후 유의한 체중 증가가 있었다. 결론: 이 연구를 통해 교체 방법에 관계없이 비교적 안전하고 용이하게 올란자핀으로 교체 할 수 있음을 알 수 있었다. 그리고 기존 항정신병약물을 올란자핀으로 교체함으로써 일부 부작용들을 줄일 수 있음을 간접적으로 관찰할 수 있었다. 하지만 이 연구는 여러 제한점과 문제점을 지니고 있기 때문에 보다 체계적인 연구를 통해 검정이 필요하리라 생각된다. Objectives: This multicenter clinical trial involving 13 hospital sites compared the safely of switching to olanzapine between ’direct switching method’ and ’start-tapering switching method’. Method: This study included both inpatients and outpatients who fulfilled the criteria for schizophrenia as defined in the ICD-10, and were in need to be appropriate for switching antipsychotics. Subjects were randomly assigned to one of the two switching methods. For ’direct switching method’group, previous antipsychotics were abruptly discontinued and 10㎎ of olanzapine was administered, and previous antipsychotics was gradually tapered for 2 weeks. Olanzapine was used for 6 weeks and the dose was adjusted within the range of 5-20㎎. The safety of switching to olanzapine was measured with vital sings including body weight, adverse events reported spontaneously, laboratory tests, and various scales such as Simpson-Angus Scale(SAS), Barnes Akathisia Rating Scale(BARS). Abnormal Involuntary Movement Scale(AIMS), and Liverpool University Neuroleptic Side Effect Rating Scale(LUNSERS). Results: 103 patients were switched to olanzapine in this study. The comparison between two switching methods did not show any significant difference in the dosage of olanzapine used, the concomitant use of benzodiazepine, the rate and reasons of drop-out, the adverse events, vital signs, laboratory tests, and most scales for measuring side-effects. However, the decrease in AIMS scores was significantly lower in ’direct switching method’ group, and the concomitant use of anticholinergics was comparatively greater in ’start-tapering switching method’ group. At baseline, SAS and BARS scores were 3.5 and 1.8 points respectively, and more than 70% of the subjects showed hyperprolactinemia. After switching to olanzapine, SAS, BARS, and AIMS scores were significantly decreased and the proportion of the patients with hyperprolactinemia was also decreased to less than 30%. However significant weight gain after the treatment of olanzapine was observed regardless of switching method. Conclusion: This study may suggest that switching to olanzapine can be done with relatively high safety regardless of switching methods and olanzapine can significantly decrease some side-effects induced by other antipsychotics.

Frequency-sensitive Diversification in Collaborative Filtering

Seok-Jong Yu(유석종) 한국정보기술학회 2015 한국정보기술학회논문지 Vol.13 No.7

Due to the rapid development of data analysis technologies, recommender systems become indispensible in online markets and results in the shrinking of traditional offline competitors. As a representative leader, collaborative filtering has been widely adopted in online markets like Amazon.com, and, unlike content-based method, which suggests unexperienced items preferred by similar neighbors of a target customer. While collaborative filtering has been developed towards the recommendation precision improvement in the past, recently the diversity of recommendation becomes another important criteria for evaluating its performance. As related works, the dissimilarity measurement between recommended items in properties, and longtail item recommendation have been suggested. For the diversity improvement, this paper argues frequency-sensitive recommendation method, which aims to extend the global diversity of items rather than local diversity suggested by the past studies. As well, it introduces a comprehensive evaluation on both the diversity and the precision of recommendation, and shows the experimental results of comparing with existing methods using the actual Movielens dataset.

Construction and analysis of the biological interaction network for BCL2

Seok Jong Yu,Jaesoo Yoo 한국콘텐츠학회 2014 한국콘텐츠학회 ICCC 논문집 Vol.- No.-

Comprehensive Temporal Filter for Expanded Collaborative Filtering Algorithm

Seok-Jong Yu 한국정보기술학회 2013 한국정보기술학회논문지 Vol.11 No.11

Collaborative filtering-based recommendation has been introduced in e-commerce systems, and it can save time and cost for retrieval large scale online items. However because traditional collaborative filtering algorithm depends on item rating scores, it is hard for recommendation systems to reflect temporal properties. In order to improve this problem, temporal decay filter has been proposed, and it mainly focuses on the elapse time of individual item after the initial rating time rather than general temporal similarity among the entire items rated in common by multiple users. From this motivation, this paper proposed the comprehensive temporal filter so that global temporal similarities of common items are considered by the recommendation algorithm, and the performance has been evaluated by the comparison with existing methods.