황색종 건조과의 저장조건에 따른 수분함량 및 색상변화

박창호 충북대학교 연초연구소 1997 煙草硏究 Vol.16 No.-

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국내산 당귀의 정유성분 조사

이태항, 홍열, 임흥빈 충북대학교 연초연구소 2008 煙草硏究 Vol.21 No.-

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농업기술혁신의 경제성 평가와 관리체계

박종섭,안인찬 충북대학교 연초연구소 2004 煙草硏究 Vol.19 No.-

This study originated from an issue that technological innovation is an important factor in modern agriculture and it should be controlled systematically by government and farms. The objective of this study is to find out the methods of economic evaluation and management system of technological innovation in agriculture. Technological progress in agriculture improves the agricultural production function. For any given amount of inputs, we get more output than we did in the past farming. Modern theories of endogenous growth attempt to explain the rate of technological progress, which the Solow model takes as exogenous. These models try to explain the decisions that determine the creation of knowledge through research and development. The growth of agricultural income per farm is determined by the rate of technological progress. Because technological innovation in modernized agriculture is very important factors increasing the revenue and reducing the production cost. The difference between production functions is due to a difference of technique in how the inputs are used in producing the output. A farmer will not adapt a change in technology unless he expects that the adaption will lead to a reduction in per unit costs at the output at which he expects to operate. Since most innovations involve additional expenditures, total costs are likely to be increased at lower levels of output as a result of technological changes. Nevertheless, in order to increase the agricultural production and the levels of living it is necessary to develope and adapt technological improvements in agriculture. The major problem in agricultural technological innovation is to evaluate the economic value of new technology adapted by farmers in agricultural accounting system and to prepare the technological management system to develope better technology. Farms should reflect the economic value of technological changes through financial analysis in agricultural accounting system. Because an essential activity in financial management of agriculture is the development of a timely technological information system that provides a means for the decision maker as a farm operator to measure, evaluate, control, and improve the financial performance of the farm business. The government should develope the education of the skilled technical hands, R&D investment, the accumulation and risk management of agricultural technological information, and the development and spread of agricultural technology. We can expect the principal role of local agricultural college in technological management system of agriculture by the decentralization of agricultural extension function.

통기방식에 의한 고려인삼의 기관분화 특성

정찬문,신주식,이이,장병용 충북대학교 연초연구소 2004 煙草硏究 Vol.19 No.-

기내 배양환경 개선방안의 일환으로 강제통기방식을 이용한 치상배의 발아유형과 생장특성 등을 구명하였다. 강제 통기방식에 의한 배양용기는 기존의 삼각플라스크 배양에 비해 결로 현상이 적었을 뿐 치상절편의 기관분화 및 생장에는 효과가 없었다. 그러나 기내의 공기를 환기시키고 GA등 양액을 추가 공급하여 배양체의 생장을 도모할 수 있는 장점이 있는 반면 오염율은 높았다. 발아유형별 기관분화는 자엽 전개가 양호한 B type이 좋았으나 자엽이 전개하지 않은 A Type과 상배축이 전개한 C Type은 불량하였다. 배(embryo)의 부위별 callus 생장은 자엽과 경근이동부가 좋았고, 배를 전체배양 했을 때 callus 노화가 쉽게 일어났다. 절편체에서 shoot 분화는 자엽과 경근이동부가 양호하였다. For the improvement of in vitro incubation conditions, we analyzed the germination and growth of zygotic embryo explant using forced ventilation. Forced ventilation did not show significant difference in organ formation and growth of zygotic embryo explant compared to triangular flask except for lower dew formation. Ventilation and GA addition to the medium increased not only the growth rate but also the contamination rate. We tested the organ formation rate according to the germination type. B type, which has good cotyledon formation, showed higher organ formation rate, but A type, which has no cotyledon, and C type, which has hypercotyl, showed lower organ formation rate. We tested the callus growth rate according to the part of embryo. Calli from cotyledon and the region between root and stem showed higher growth rate. Calli induced from whole embryo showed early senescence. Cotyledon and the region with root and shoot showed good explant shoot formation.

분자마커를 이용한 작물의 품종 개량과 품질관리

이이,엄유리,정찬문,석영선,이학수 충북대학교 연초연구소 2009 煙草硏究 Vol.22 No.-

분자마커는 현대 농업에서 없어서는 안 될 중요한 도구로 자리잡아가고 있으며 단백질과 DNA에 기초한 다양한 분자마커가 개발되었다. RFLP, RAPD, SSR, AFLP, CAPS, SNP, STS, DNA sequencing 등 다양한 분자마커는 독특한 특성을 가지고 있어서 용도에 맞게 분자마커를 선별하여 사용할 필요가 있다. 분자마커의 선별에서는 사용가능한 DNA의 양, DNA의 품질, 공우성 마커 여부, 비용 등을 고려해서 해야 한다. 담배에서는 주로 병저항성과 품종을 감별하기 위한 분자마커가 개발되어 이용되고 있으나 담배의 DNA 다형성이 다른 작물에 비하여 현저히 낮아 게놈프로젝트가 완성되면 resequencing을 이용하여 마커를 개발하는 것이 효율적일 것으로 보인다. Molecular marker is very important tool for modern agriculture. Various molecular markers were developed for detecting protein and DNA polymorphism. Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Simple Sequence Repeat (SSR), Amplified Fragment Length Polymorphism (AFLP), Cleaved Amplified Polymorphic Sequence (CAPS), Single Nucleotide Polymorphism (SNP), Sequence Tagged Site (STS) and DNA sequencing are typical molecular markers commonly used. Each molecular marker has its own specific characters. Researchers have to choose a molecular marker for their own demands with considering the DNA quantity required, DNA quality required, codominance, and cost for developing and analysis. In tobacco, disease resistance and cultivar identification are the main focus of marker development. DNA polymorphism of tobacco is much less than that of other crops. Completion of genome project followed by resequencing could be a good opportunity for molecular marker development of tobacco.

담배 유전공학의 현재와 미래

이이 충북대학교 연초연구소 2004 煙草硏究 Vol.19 No.-

담배 품종의 개발에 있어서 유전공학적 기술의 이용에 관하여 미국과 유럽에서 발행된 문서와 각종 database를 중심으로 조사하였다. 담배는 2002년까지 미국과 유럽에서만 297가지 이상의 형질전환체가 만들어져서 재배시험을 거쳤으며 미국에서는 병해 저항성 유전자를 중심으로 형질전환이 이루어 졌으며 유럽에서는 여러 가지 형질에 대해서 형질전환이 골고루 이루어 졌다. 그중 oxinyl계 제초제 저항성을 가지는 C/F/93/08-02와 nicotin함량이 저하된 Vector 21-41이 지금까지 상업적으로 허가되었다. 콩, 옥수수, 면화, 유채에 비하여 형질전환체가 재배되는 면적이 미미한 형편이나 담배는 여러 가지 특성에 의하여 의약용단백질을 식물에서 생산하는 생물 공장으로서의 가능성이 높게 평가되고 있다. 그러나 다른 주요 식물에 비하여 담배는 유전자서열이 많이 밝혀지지 않은 상태이다. I studied various databases and documents to study the roles of biotechnology in tobacco cultivar development. 297 transformants were made and approved field test only in United States and European Union by 2002. In United States, transformations were tried mainly for the resistance to pathogens and in European Union, that were done for various traits. C/F/93/08-02, which has resistance against oxinyl family herbicide, and Vector 21-41, which has lowered nicotine contents, were commercially approved until now. The cultivation area of transformed tobacco is much less than the area of soybean, maize, cotton, and rape. For many advantages of tobacco, tobacco is a good candidate plant for biofactory for pharmaceutical protein production. The genomic DNA sequence of tobacco is not revealed as large as other major crops.

측백나무 잎의 정유성분 조사

임동균, 홍열, 임흥빈 충북대학교 연초연구소 2008 煙草硏究 Vol.21 No.-

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한국산 벽오동 씨앗의 정유 성분

곽호근, 홍열, 임흥빈 충북대학교 연초연구소 2008 煙草硏究 Vol.21 No.-

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국내에서 생산 판매되는 담배 각초에 존재하는 보습제 함량 비교

박병주,임흥빈,이학수 충북대학교 연초연구소 2006 煙草硏究 Vol.20 No.-

This study was carried out to compare humectant contents existed in tobacco shred from 69 cigarette brands being on sale in Korea. Humectant in tobacco shred were extracted by AOAC official method and analysed by gas chromatography. Mean value of glycerol, propylene glycol, and triethylene glycol contents from 69 cigarette brands were 13.6 ± 4.5 mg/g, 6.5 ± 2.6 mg/g, and 0.8 ± 0.2 mg/g, respectively. Humectant contents existed in tobacco shred of cigarette brands manufactured by small tobacco companies were relatively lower than that of the other four major tobacco companies in Korea. These results indicates that humectant contents in tobacco shred from cigarette have to determine periodically in order to give a basic information for quality evaluation of cigarette smoke in connection with smoking and healths, because it plays an important role the formation toxic cigarette smoke components. 본 실험은 국내에서 시판되고 있는 69종의 궐련담배 각초를 재료로 하여 실시하였다. 궐련담배 각초에 함유되어 있는 보습제의 함량을 측정하고 궐련담배 주류연의 tar 및 nicotine 함량을 측정하여 비교분석한 결과는 다음과 같다. 1. 국내에서 시판되는 귈련담배 각초 보습제 성분의 평균함량은 glycerol 13.6±4.5 mg/g, propylene glycol 6.5±2.6 mg/g, triethylene glycol 0.8±0.2 mg/g이었다. 2. 군소업체 귈련담배 각초에 존재하는 보습제 함량은 대규모업체에 비해 상대적으로 매우 낮은 수준을 유지하고 있었다

한국산 후지사과 Polyphenol Oxidase의 특성 및 활성억제

오동주, 홍예현, 곽호근, 임흥빈 충북대학교 연초연구소 2010 煙草硏究 Vol.23 No.-

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