고혈압쥐에서 성장에 따른 신장 세포막 Na, K-ATPase와 Adrenergic Receptor의 변동에 관한 연구

박성규,이재흔 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

To investigate the developmental changes of Na, K-ATPase and adrenrgic receptors in the kidney basolateral membrane(BLM) of hypertensive rat, Na, K-ATPase activities, [^3H]ouabain, [^3H]prazosin and [^3H]dihydroalprenolol(DHA) binding sites were measured in the kideny basolateral membrane prepared from 1, 3, 5, 10 and 15 weeks old Sprague-Dawley rats(SD) and spontaneously hypertensive rats(SHR). Results are as follows ; 1. Systolic blood pressure in SHR was not elevated at 3 and 5 weeks old, but elevated significantly to 185 mmHg at 10 or 15 weeks old. 2. Na, K-ATPase activity of BLM in 3 weeks old SD was markedly increased compared to that in 1 week old and there was little change in 5, 10 or 15 weeks old rat. 3. Na, K-ATPase activity of BLM in SHR was also markedly increased at 3 weeks old, but continuously increased by 15 weeks old. Although the Na, K-ATPase activity in BLM of 1, 3 or 5 weeks old SHR was lesser, that of 10 or 15 weeks old SHR was higher than that observed in corresponding age of SD. 4. The numbers of ouabain binding sites were increased in both groups with increasing age. In the SHR, the numbers of ouabain binding; sites were lower in 1, 3 or 5 weeks old but higher in 10 or 15 weeks old, than those in corresponding age of SD. 5. In the dose-response curves of ouabain sensitive Na,K-ATPase activity and the ouabain binding experiments, Na, K-ATPase in the BLM of SD or SHR was consistent with the putative existence of low affinity site(IC_50 : 35 - 55 μM, Ed : 40 - 90 μM) 6. α1-Adrenergic receptor densities([^3H] prazosin binding sites) were markedly increased by 3 weeks after birth and further increased slightly by 5, 10 or 15 weeks old SD. 7. The numbers of prazosin binding sites in SHR were also increased with increase of age. However those were lower in 1, 3 or 5 weeks old, higher in 10 or 15 weeks old SHR than those in corresponding age of SD. 8. In the prazosin binding experiments, Ed value was 0.1 - 0.4 nM and there was no difference between in Kd values of SD and SHR. 9. β-Adrenergic receptor densities([^3H] DHA binding sites) in SD were markedly increased in 3 weeks old compared to those in 1 week old and there were no further changes of receptor densities in 5, 10 or 15 weeks old. But those in SHR were still increased until 15 weeks old. The numbers of DHA binding sites in 1, 3 or 5 weeks old SHR were slightly lower but almost same in 10 or 15 weeks old, compared to those in corresponding age of SD. 10. In the DHA binding experiments, Kd value was 1.0 - 1.8 nM and there was no difference between in Kd values of SD and SHR. From the above results, it is suggested that as increase of age, Na, K-ATPase, α1 and β-adrenergic receptors in the kidney basolateral membrane of SHR were increased with different pattern from normotensive SD ; lower in young age, but same or higher in hypertensive period of SHR than SD. These observed changes in Na,K-ATPase activity and adrenergic receptor densities of kindney basolateral membrane may partly contribute to the regulation of blood pressure in SHR.

신경교세포 및 RAW 264.7 세포에서 Protein kinase의 활성에 의한 유도성 Nitric oxide synthase의 발현

박상철,노삼길,배소현,박지선,이충재,허강민,석정호,이재흔 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

NO(nitric oxide) plays an important role as neurotransmitter or cytokine, and pathologic factor for some diseases by the large amount production with iNOS(inducible NO synthase) expression in macrophages or glial cells. The expression of iNOS is regulated by various cytokines, protein kinases and transcription factors. In this experiment, to investigate the roles of progein kinase and NF-kB for iNOS expression, the effects of PMA(phorbol 12-myristate 13-acetate), cAMP, and various protein kinase inhibitors on LPS(lipopolysaccharide)-induced iNOS mRNAN expression and nuclear NF-kB binding complex were examined in C6 glial cells and RAW 264.7 cells. In C6 glial cells, iNOS mRNA expression by LPS was induced from 1 hour and peak at 3 hour after treatment. In RAW 264.7 cells, the mRNA was observed from 3 hour and peak at 6 hour. PMA enhanced markedly LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but did not much influence on LPS-induced iNOS mRNA expression in RAW 264.7 cells, in spite of increased LPS-induced NF-kB binding complex at 30 min. cAMP(dibutyryl cAMP) did not much influence on LPS-induced iNOS mRNA expression, by increased LPS-induced NF-kB binding complex in C6 glial cells. However, in RAW 264.7 cells, cAMP increased slightly LPS-induced iNOS mRNA expression without change of NF-kB binding complex. Staurosporine did not influence on LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased LPS-induced iNOS mRNA expression and NF-kB binding complex. Ro-31-8220 did not much influence on LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased significantly LPS-induced iNOS mRNA expression in spite of increased LPS-induced NF-kB binding complex for 3hours. G 6976 did not much influence on LPS-induced iNOS mRNA expression with decreased NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased iNOS mRNA expression without influence on LPS-induced NF-kB binding complex. Genistein did not influence on LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased LPS-induced iNOS mRNA expression inspite of increased NF-kB binding complex. These results suggest that LPS-induced regulation of iNOS expression or NF-kB activity in C6 glial cells, might be different from RAW 264.7 cells through various protein kinases or other factors.

이식형 중이용 청각보조 트랜스듀서 개발에 관한 연구

曺辰鎬,朴世光,李相欣 경북대학교 센서기술연구소 1995 연차보고서 Vol.1995 No.-

청각보조용 이식 중이를 위한 코일-영구자석형의 장치들을 체외기와 체내기로 나누어 설계 및 제작하고 실험하였다. 이식 중이용의 코일과 자석으로 된 진동부의 설계를 정량화시키기 위하여 중이와 진동체를 포함하는 일련의 시스템을 모델화시켜 해석하였다. 제작된 시스템의 체내기는 수신용 코일, 검파회로, 진동부 및 지지 샤프트 등으로 이루어지며 체외기는 음성 증폭부, 발진 및 변조부로 구성된다. 제작된 체내기를 고막과 청소골 및 내이가 보존된 사체의 측두골에다 이식한 다음 주파수 변화에 따른 청소골의 미소 진동을 진동 측정기로써 측정한 결과 각각 400㎐ 및 2.7㎑에서 피크를 갖는 곡선을 얻음으로써 이식형 중이의 실현 가능성을 확인하였다. 또한 전력 소아 및 다이나믹 레인지들을 고려하여 체외부인 송신부를 설계 제작하였으며 이들을 케이스를 포함하여 도합 18.5g의 귀걸이형으로 만들었다. We designed, developed and experimented coil-permanent magnet type device for implantable middle ear. We made a model of whole system including middle ear and vibrator and analyzed in order to quantificate design of vibration part, consisted of coil for middle ear and magnet. Internal unit consists of receiver coil, demodulation circuit, vibrator and supporting shaft and external unit consists of voice amplifier, oscillation and modulation circuit are developed respectively. After system developed, internal unit was implanted at temporal bone of carcass, minute vibration according frequency variation was measured by a vibration meter. Then, we confirmed the possibility of realizing middle ear implant by obtaining the curve whose peak values appear at 400㎐ and 2.7㎑, respectively. In addition, external unit was designed and developed considering power dissipation and dynamic range and it is an earring type device of 18.5g including case.

돈사 폐수의 물리화학적 처리에 관한 연구

이수구,박상헌 서울産業大學校 1992 논문집 Vol.36 No.1

This study was performed to treat the swine wastewater from the small scale farms. As physicochemical treatment methods, coagulation, activated carbon adsorption, Fenton reagent's reaction and dewatering of sludge were applied to remove organic materials. Adding polymer Yangfloc Ycx-4 could increase effectively COD removal efficiency above 80% and the optimum dosage of polymer was 50㎎/L. In order to remove COD, powdered activated carbons were more effective than granular activated carbons and PAC absorption results could applied well to Freunich adsorption isotherm. In Fenton reagent's reaction, COD removal efficiency was low within 30%. In the chemical treatment, killing efficiencies of E. coli were 99.5% and 99.8% at pH 11 and 12, respectively. The dewaterability of sludge could be increased effectively by adding lime and cationic polymer.

鮟鱇網 漁具 漁法의 改良에 관한 硏究(Ⅱ) : 展開帆을 이용한 改良漁具 試驗(豫報)

李吉來,宋之浩,朴鍾洙,徐萬錫,趙鳳坤,朴正鎬,洪聖根,鄭公炘,裵錫悌,鄭與基,金鎭乾 군산대학교 1980 群山水産專門大學 硏究報告 Vol.14 No.1

Ordinarily stow net fisheries was used by floater beam and sinker beam for the purpose of spreading the mouth of net. But it was not only iuconvenient and dangerous but much defective. In this thesis, we have studied fundermental experiment of the improved stow net fishing gear using head rope, ground rope and otter sail instead of floater beam and sinker beam. From the result of this experiment, it has been succeeded for fishing work to achieve basically without any difficulty. The results obtained in this study were as follows. 1) The resistance of fishing gear was estimated R≒7V2 as possible as it is taken greatly. (R: Resistance V: the rate of current) 2) The interval of otter sail was spreaded smoothly. Being in normal condition, it was maintamed the width 37-38m. It was heared comparison with net mouth of ordinary gear, and the shearing force was enough. 3) The height of mouth was lower than we have anticipated to and seemed to have the supplement of buoyancy. But according to increasing the rate of current, the lowness of mouth height was little affected. 4) The height of otter sail was decreased at the rate of 30% by the rate of current, on the contrary, the height of wing was not decreased owing to sufficient buoyancy, 5) The adjustment of extention length of hanging wire(connected with fishing boat) and raising wire(with otter sail) was great important problem and it must be dealed with the most moderatly because of a corelation between the spreading of fishing gear and catching ability. 6) On the depth of 30m, it was taken 25 minuits to cast anchor and lines, 20 min cast net, 24 min haul net, 45 min haul lines and heave anchor, respectively. 7) The improved stow net with otter sail was cleared up the defects and difficult problems in ordinary gear using floater beam and sinker beam, and it have more advantages of fishing work than ordinary one. But there were involved in lots of problems in details to solve in the future.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

일차배양된 설치류 호흡기 상피세포로부터의 점액소 분비에 대한 수종 약물의 영향

이충재,석정호,이재흔,허강민,박지선,배소현,노삼길,박상철 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

1. PKC activator인 PMA는 일차배양 HTSE세포로부터의 뮤신분비를 0.1μM 농도에서 30%, 1μM 농도에서 80% 가량 증가시켰다. 2. 식물 유래 성분으로, flavonoid의 일종인 TFR은 일치배양 HTSE 세포로부터의 뮤신분비를 10μM 농도에서 50%, 100μM 농도에서 80% 가량 증가시켰다. 3. 양이온성 폴리펩티드인 PLL 및 PLA는 일차 배양 HTSE 세포로부터의 뮤신분비를 0.01 - 10μM 농도에서 용량의존적으로 감소시켰다. 4. 결론적으로, 본 연구에서 얻어진 결과들은 새로운 거담제 및 점액용해제나 단백분해 효소제가 아닌 호흡기 류신의 생성/분비를 조절해 줄 수 있는 신개념의 약물을 개발함에 있어 극히 일부분이나마 단서를 제공하고 있다고 하루 수 있을 것이다. In the present study, we tried to investigate whether phorbol myristate acetate(PMA), trihydroxymethoxy-flavanone rutinoside(TFR) and cationic polypeptides significantly affect mucin release(secretion) from cultured hamster tracheal surface epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hr and chased for 30 min in the presence of each agent to assess the effect on 3H-mucin release. The results were as follows : (1) Both PMA and TFR significantly increased mucin release from cultured HTSE cells ; (2) Cationic polypeptides including po1y-L-lysine(PLL, mw 7,500) and poly-L-arginine(PLA, mw 10,800) significantly inhibited mucin release from cultured HTSE cells, in a dose-dependent manner. This finding suggests us that PMA and TFR be further studied for the possible use as mild expectorants and cationic polypeptides might function as a regulator for hyper-secretion of mucus, both by direct acting on airway mucin-secreting cells, during the treatment of chronic airway diseases.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Assessing Soil Fertility Status of Edible Wild Plants Fields in Ulleung Island

Park, Sang-Jo,Park, Jun-Hong,Kim, Byung-Sung,Chung, Yun-Hak,Lee, Dong-Jun,Kwon, Oh-Heun,Park, So-Deuk,Lee, Suk-Hee 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.4

The perennial edible wild plants such as Aster glehnii, Solidago virgaurea subsp. gigantean, Allium ochotense, Athyrium acutipinnulum, Aruncus dioicus var. kamtschaticus and Codonopsis lanceolata have cultivated as the main income crops introduced into the fields about 30 years ago in Ulleung island. Soil samples were collected from 190 fields and assessed the effects of management practices on soil chemical properties at wild edible plant fields under no-till system. The strong acidic soils of pH 5.4 or less were detected in 45% of the soil samples. The level of soil organic matter was being held at mean $63{\pm}28g\;kg^{-1}$, 2.7 times higher than upland soils in Korea. Available phosphate and exchangeable potassium showed more than recommended levels of upland crops as $680{\pm}489mg\;kg^{-1}$ and $1.94{\pm}1.7cmol_c\;kg^{-1}$, respectively. The fields of Solidago and Aster showing strong soil acidity and high level of available phosphate and water soluble $NO_3{^-}$ were distinguished from other crops in analysis of variance and principal component analysis of soil chemicals. These results suggested that high frequency of acidic soil and high levels of available $P_2O_5$, exchangeable $K_2O$ and water soluble $NO_3{^-}$ were accompanied with the use of urea and NPK-fertilizer based on nitrogen in the field. However, further research is needed to understand the appropriate management of fertilization and the prevention of soil acidification for wild edible plants.

Infective endocarditis caused by Gemella sanguinis in a hemodialysis patient

Sang Heun Lee,Deok Kyu Cho,Hyuk Min Lee,Sang Choel Lee,In Tae Kim,Hyung Bok Park,Yuk Yung Hyun,Yoon Ji Kim,Kkot Sil Lee 대한내과학회 2009 대한내과학회 추계학술대회 Vol.2009 No.-