사람의 제대정맥에서 분리한 내피세포의 배양 특성

유승모,송호연,이강순 순천향대학교 1993 논문집 Vol.16 No.2

Endothelial cells were isolated from the umbilical vein by collagenase digestion of freshly obtained human umbilical cord. These cells were grown in gelatin coated culture flask as a homogeneous population of polygonal cells with a centrally located nucleus for periods up to 4 weeks and 3-4 serial passages. By Immunofluorescent stain, von Willebrand factor were detected in both cultured endothelial cells and umbilical cord tissue. By transmission electron microscopy, Weibel-Palade bodies were observed in cytoplasm of endothelial cells as rod shape containing fine tubular structure. In conclusion, Endothelial cells of human umbilical vein treated with 0.2% collagenase were successfully cultured and it is suggested that 2 to 3 serial passages of cultures are ideal for the research of the endothelial cells..

Gemfibrozil이 정상 성인의 기분 및 신경인지기능에 미치는 영향

유승호,장순주,김진세,정인과 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.2

Objectives : Many studies have reported that the mood and cognitive functions are related to serum lipid concentration. The current study sought to determine whether gemfibrozil, lipid-lowering agent, affects mood and neurocognitive functions in normal adults. Methods : Subjects were 15 healthy medical students and gemfibrozil of 600mg administered to them daily for 4 weeks. The levels of serum total cholesterol, high lipoprotein(HDL), low-density lipoprotein(LDL), and triglyceride were measured before the treatment and after 4weeks of the treatment. The depressive mood and anxiety were estimated from Beck Depression Inventory and Spielberger State and Trait Anxiety Inventory at the baseline and the 4th week. Neurocognitive functions were estimated from digit span test, digit symbol substitution test, finger tapping test, visual continuous performance test and cued test at the baseline and the 4th week. Results : After four weeks, the subjects administered gemfibozil showed significant reductions in serum triglyceride levels(p=0.033), but other lipid levels did not show significant changes during the treatment. During gemfibrozil administration, no statistically significant changes were found in Beck Depression Inventory scales, Spielberger State and Trait Anxiety Inventory and other neurocognitive scales except for the digit symbol substitution test(p=0.000) between the baseline and following the 4th week. Conclusions : Even though we need more well-controlled and long-term follow-up study in various and a large number of groups, these findings suggest that the gemfibrozil administration do not affect the mood and the neurocognitive functions comprehensively.

원발성과 이차성 기흉에서 비디오흉강경수술의 임상결과

최순호,류대웅,이미경,이삼윤 원광대학교 의과학연구소 2009 圓光醫科學 Vol.24 No.1

배경: 젊은 성인에서 자발성기흉의 치료는 논란이 많다. 흉관삽관술로 구성된 고식적인 치료는 흉관 삽관시기의 이환율, 장기간의 입원 그리고 일정기간 안에 수술을 요한다. 젊은 성인에서 기흉은 보통 폐 첨부의 기포를 동반한 것처럼, 저자는 첫 기흉의 발생 시기에 기포의 흉강경하 절제는 낮은 이환율과 짧은 입원 일을 동반하는 효과적인 치료이며 개흉술에 대한 훌륭한 대체수단이라고 가정하였다. 대상 및 방법:2005년 1월부터 2006년 12월까지 166명의 기흉환자에서 비디오흉강경수술을 시행하였다. 155명은 남자이었고 11명은 여자이었다. 평균 나이는 29.3±6.7세(15-75세)이었다. 환자는 특히 날씬하고 키가 컸으며 증상의 발현시기에 경도의 호흡곤란과 흉통을 보였다. 폐 첨부의 기포는 조직 검사로 확인을 하였다.130명의 환자는 5일이상의 지속적인 공기누출과 흉부 컴퓨터사진상 다발성 기포 때문에,15명은 반대편 기흉으로, 7명은 양측성 기흉, 그리고 5명은 흉관삽관술의 치료 후 재발한 경우로 수술을 받았다. 모든 환자에서 Endo-GIA stapler 에 의한 폐 쐐기절제수술과 탈크도포, 그리고 늑막찰과상이 수행되었다. 추적 동안에 환자의 재발 과 잔존 만성 흉통을 관찰하였다. 결과: 수술사망은 없었다. 수술 후 합병증은 1명에서 과도한 늑막찰과상에 기인한 출혈로 재수술,9명의 환자에서는 7일 이상의 지속적인 공기누출, 그리고 3명에서 재발을 보였다. 재발은 수술 후 1-3개월 동안에 발생하였다. 그리고 또한 16명에서 경도의 잔존 흉통을 호소하였지만 진통제는 필요하지 않았다. 입원 일은 원발성 기흉 환자에서는 5.2±2.4일 그리고 이차성 기흉환자는 7.5±3.2일 이었다. 결론: 늑막유착술을 동반한 폐 첨부의 쐐기절제수술은 낮은 이환율과 짧은 입원 일을 동반하고, 초기 흉관삽관술이나 개흉수술 같은 고식적인 수술에 대한 매력적인 대체수단이다. 흉강경수술은 자발성 기흉 환자를 위한 효과적으로 처음 시도하는 수술일 수 있다. 이 질환의 병태생리상 기흉 환자는 기흉의 재발 때문에 엄밀한 추적을 요한다. Background:The treatment of spontaneous pneumothorax in young adult has been controversial. Conventional treatment consisting of chest tube thoracostomy may be associated with morbidity at the time of tube insertion, prolonged hospitalization, and interval operation in many patients. As spontaneous pneumothorax in young adults is usually associated with apical blebs, we hypothesized that video-assisted thoracic surgery(VATS) resection of the blebs at the time the first pneumothorax may be effective treatment associated with low morbidity and short hospital stay, and also viable alternative to thoracotomy. Method & materials. From Jan.2005 to Dec.2006,a series of 165 patients with spontaneous pneumothorax were treated by video-assisted thoracic surgery, there were 135 men and 11 women. The mean age was 29.3±6.7 years old(range:15to 78 years).Patients were predominantly tall and thin and also mildly symptomatic at the time of presentation. Apical bullae was confirmed in the pathologic specimen.139 patients was treated for persistent air leak(>5days) and multiple blebs & bullae on Chest CT, 15 patients for contralateral pneumothorax. 7 patients for bilateral pneumothorax, and another 5 patients for recurrent pneumothotax after conservative treatment. Stalpling of blebs and bullae with Edo-GIA stapler and pleurodesis by Talc poudrage and pleural abrasion was performed in all cases. During follow-up patients were observed for recurrence and residual chronic chest pain. Results: There was no postoperative death. Postoperative complications were reoperation due to excessive pleural abrasion in one patient and continued air leaks more than 7 days in 9 patients, and recurrence in 3 patients.Recurrence were occurred during postoperative 1 to 2 months also mild chronic residual chest pain also noted in 16 patients, but no required analgesics. The hospital stay was mean 5.2±2.4 days in primary spontaneous pneumothorax and mean 7.5±3.2 days in secondary pneumothorax. Conclusions: VATS wedge resection of apical bullaes with pleurodesis is associated with low morbidity and short hospitalization, and provides an attractive alternative to the conventional treatment of initial tube thoracostomy and possible interval repeat thoracostomy or operations. VATS may be an effective first line treatment for spontaneous pneumothorax.Due to the pathophysiology of this disease, patients should be closely followed for the occurrence of the pneumothorax.

정중운동신경과 척골운동신경의 전기생리학적 연구

김종순,이현옥,안소윤,구봉오,남건우,김영직,김호봉,류재관,류재문 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

The determination of peripheral nerve conduction velocity is and important part to electrodiagnosis. Its value as neurophysiologic investigative procedure has been known for many years but normal value of median and ulnar motor nerve was poorly reported in Korea. To evaluate of median and ulnar motor nerve terminal latency, amplitude of CMAP(compound muscle action potential), conduction velocity and F-wave latency for obtain clinically useful reference value. 71 normal volunteers(age,19-65 years;142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrine disorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking Ⅱ was use for detected terminal latency, amplitude of CMAP, conduction velocity and F-wave latency of median and ulnar motor nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation, independent t-test was used to compare between Rt and Lt side also compare between different in genders. The results are summarized as follows: 1. Median motor nerve terminal latency was right 3.00ms, left 2.99ms and there was no significantly difference between right and left side and genders. 2. Median motor nerve amplitude of CMAP was right 17.26mV, left 17.50mV and there was no significantly differences between right and left side and genders. 3. Median motor nerve conduction velocity was right 57.89m/sec, left 58.03m/sec and there was no significantly difference between right and left side and genders. 4. Median motor nerve F-wave latency was right 25.74ms, left 25.59ms and there was significantly differences between genders. 5. Ulnar motor nerve terminal latency was right 2.38ms, left 2.45ms and there was significantly differences between right and left side. 6. Ulnar motor nerve amplitude of CMAP was right 15.99mV, left 16.02mV and there was no significantly differences between right and left side and genders. 7. Ulnar motor nerve conduction velocity was right 60.35m/sec, left 59.73/sec and there was no significantly differences between right and left side and genders. 8. Ulnar motor nerve F-wave latency was right 25.53ms, left 25.57ms and there was significantly differences between genders.

척골 지단 신경의 전기생리학적 연구

김종순,이현옥,안소윤,구봉오,남건우,김호봉,류재관,류재문 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

The ulnar nerve extends down the arm, across the elbow, and into the hand. It provides sensation to the little and ring fingers and activates many of the small muscles in the hand. The determination of peripheral nerve conduction velocity is an important part of ulnar nerve evaluation. The electrodiagnostic value as neurophysiologic investigative procedure has been known for many years but normal value of digital nerve was not reported in korea. The purpose of this investigation was to measure the digital nerve conduction velocity of ulnar nerve for obtain clinically useful reference value and compare difference in each fingers and then compare with the other countries. 71 normal Korean volunteers (age, 19-65 years; 142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrinedisorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking Ⅱ(EMG machine) was use for detected conduction velocity and amplitude of digital nerve in ulnar nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation and independent t-test was used to compare with ring and little finger. Conduction velocity of the right ring finger was 57.44m/sec and little finger was 55.32msec. The left ring finger was 55.55msec and little finger was 54.11msec. Amplitude of the right ring finger was 30.28µV and little finger was 48.36µV. The left ring finger was 30.67µV and little finger was 57.76µV. There were significantly difference between ring and little in amplitude (p<.05) but there were no statistically difference between conduction velocity of ring and little finger (p>.05). The amplitude of little finger are greater than ring finger. The present results revealed that electodiagnosis can easily perform in little finger for digital nerve of ulnar nerve study.

걷기운동과 걷기운동 후 진동운동 병행 방법이 중년 비만 여성의 신체조성에 미치는 영향

박순호,류범선 龍仁大學校 體育科學硏究所 2008 體育科學硏究論叢 Vol.17 No.1

In the present study, the effects of walking exercise applying whole body vibration exercise on a body composition(Weight, %Fat, Fat, Body Mass Index; BMI, Soft Lean Mass, and Waist-Hip Ratio; WHR) change in a middle-aged obese female, and to figure out which method are more effective on those variables was investigated. Subjects were divided into two groups: the walking group(A, n=9) and the walking+whole vibration group(B, n=8). In an intensity of walking treadmill exercise was same in each group(HRmax60%), however A group has treated just walking on the treadmill with 40min and B group has 30min walking and applied 10min of whole vibration exercise. The results are as follows; 1. After 8 weeks training, there were no significant changed on weight, BMI, %Fat and Soft Lean Mass in A group, however, it have shown to decrease at Fat(kg) and WHR. And B group have appeared to have decreased at weight, BMI, %Fat, Fat(kg) and WHR, 8 weeks of training, respectably, except Soft Lean Mass. 2. After 8 weeks training, there were no significant reduction showed between groups. Overall, its applying whole vibration exercise with walking seemed to be effect on a decomposition of body fat, and these results suggested to apply those kind methods should include aerobic exercise, such as walking and running etc, for increasing the its effects.

주기적 정성적분석을 통한 훈련목표 제공이 남자 역도 인상기술 향상에 미치는 영향

문영진,류중현,이순호 한국운동역학회 2004 한국운동역학회지 Vol.14 No.2

Y. J. MOON, J. H. RYU, S. H. LEE. Korean Journal of Sport Biomechanics, Vol. 14, No. 2, pp. 69-83, 2004. The purpose of this study is searching for technical merits and demerits of each weight lifting player through qualitative motion analysis system. Moreover, It is also analysis the repeating the establishment of exercise purpose and studying for the effect of the field adaptation. The subject of this study was five male weight lifting players who have been engaged in Korean Delegation Team. The institution of exercise target was made through two times qualitative analysis and the result of studying for the effect of the field adaptation was produced before offering feedback. Moreover, two time analysis added after offering feedback. All analysis was based on 2-D visual analysis. The results of this study are as follows: 1. Maximal barbell moving speed in starting phase was decreased after offering feedback. This result implies advancement of technical skills after offering feedback. 2. From starting posture to 앉아받기, forward and backward moving distance of hip joint was decreased after offering feedback in all subjects. This result represents advancement of technical skills after offering feedback. 3. In terms of pull phase, forward and backward moving distance of hip and shoulder joint was decreased after offering feedback in all subjects. This result represents advancement of technical skills after offering feedback. 4. In terms of pull phase, the difference of horizontal value of coordinates was decreased after offering feedback in all subjects. This result represents advancement of technical skills after offering feedback. 5. In terms of pull phase, the motion range of hip joint was decreased after offering feedback in three of five subjects and this represents advancement of technical skills after offering feedback. However, the rest of them were not variable or narrow decreasing. This result represents that feedback system could not brought tremendous effects. 6. From apex point of barbell to ? 6} =?j, the difference of barbell height was decreased after offering feedback in three of five subjects and this represents advancement of technical skills after offering feedback. However, the rest of them weren't variable or narrow increasing. This result represents that feedback system could not brought tremendous effects. 7. In terms of last-pull phase, the angular velocity of knee joint was increased after offering feedback in four of five subjects and this represents advancement of technical skills after offering feedback. However, the rest of them, only one subject, decreased. This result represents that feedback system could not brought tremendous effects. 8. In terms of last-pull, the conversional tendency of maximal extension to flextion came out all but simultaneously without offering feedback in four of five subjects. This is well-performed technique. Only one subject, however, could not use power effectively because the fact that his maximal extension came out in serial, from ankle to knee and waist means dispersion of power. In addition to, after offering feedback, only one subject made increasing the maximal extension of knee in last-pull and this result represents advancement of skills after offering feedback. However, the rest of them could not make meaningful development after offering feedback. 9. It might be assumed that searching for technical merits and demerits of each weight lifting player through qualitative motion analysis system could improve player's skill.

Bacilus Calmette Guerin의 可移植性腫瘍 마우스의 壽命에 미치는 影響

全南昊,柳榮海,金舜姬,尹鐸求 國立保健硏究院 1975 국립보건연구원보 Vol.12 No.-

성공적인 SCM을 위한 공급사슬 파트너십의 구조적 관계 모형에 관한 연구

김재전,박형호,유일,소순후 한국정보전략학회 2003 한국정보전략학회지 Vol.6 No.1

Supply Chain Management(SCM) is one of the key issues, trands and needs in the current business environment. The concept of SCM emphasises collaboration among the firms within a given supply chain. Many executives are developing supply chain partnership in an attempt to reduce costs, improve service and gain competitive adveatage. The objective of this paper is to proposes a conceptual framework for empirical investigation that shows how and to what supply chain partnership can occur in SCM environment. For this purpose, the paper conceptualizes supply chain partnership in terms of sentimental components(relationship commitment and trust) and behavioral components(flexible adaptation and collaboration). And the conceptual framework identifies strategic fit, relationship interdependence, compatibility, and communication as determinants of sentimental components and behavioral components. Furthermore, the model includes expected performance consequences of supply chain partnerships.

담도 및 췌장 악성종양에서의 CAl9-9의 진단적 가치에 대한 평가

명승재,최승목,김은옥,최호순,이성구,김명환,민영일,류진숙 大韓消化器病學會 1995 大韓消化器病學會誌 Vol.27 No.3