Experimental and Numerical Research on Uplift-Restricted and Slip-Permitted Screw-Shaped Connectors

Yuxuan Chen,Quansheng Yan,Xiaolin Yu,Buyu Jia,Yu Wu,Yufan Luo 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.1

Improving mechanical performance of the negative moment regions of continuous composite beams is essential for composite beam bridges. Uplift-restricted and slip-permitted (URSP) screw-shaped connectors can enhance the crack resistance of a concrete slab through free slip between the concrete slab and the steel beam. Furthermore, URSP screw-shaped connectors are more adaptable to the requirements of rapid construction than URSP T-shaped connectors. To investigate the slip and mechanical behavior of URSP screw-shaped connectors, nine push-out tests with various parameters were conducted. In these tests, the thickness of the foam sleeve wrapped outside the screw exerted a highly signifi cant eff ect on free slip performance. A refi ned 3D nonlinear fi nite element model with the foam’s constitutive model was established using ABAQUS. The stress mechanism and failure modes of the FE models were analyzed. Lastly, to estimate the slip performance of URSP screw-shaped connectors, an equation with a simple form was proposed via mechanism analysis and a regression method.

Experimental and Mechanical Behavior of Rubber-Sleeved Group Studs

Yuxuan Chen,Quansheng Yan,Buyu Jia,Xiaolin Yu,Yu Wu,Yufan Luo 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.9

The group studs used in precast steel-concrete composite bridges can reduce the volume of cast-in-place concrete, consequently accelerating the construction process and improving the construction quality. However, the small spacing of the studs in the group studs may result in a decrease in shear strength and ductility, and a nonuniform distribution of shear force at the root of the studs. Hence, rubber-sleeved group studs, whose studs are wrapped by a rubber sleeve at the root, are proposed to redistribute the shear force at the steel–concrete interface and improve the ductility of the shear connector. In this study, four push-out tests were conducted to demonstrate the composite mechanism of rubber-sleeved group studs. Results show that the rubber-sleeved group studs exhibit better ductility than ordinary group studs. Finite element models verified using the test results were also established for parametric analysis. The mechanism analysis shows that the concrete compression regions around stud roots are sufficiently utilized. Based on the parametric analysis, designing suggestions for rubber-sleeved group studs were provided.

Motion blur processing method for visual SLAM system based on local residual blur discrimination network

Jiahao Chen,Yehu Shen,Qixin Zhu,Quansheng Jiang,Ou Xie,Jing Miao 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7

In visual simultaneous localization and mapping (vSLAM) systems, motion blur often leads to insufficient number of matched features, resulting in tracking failure. Existing solutions often tackle this problem by restoring sharp images from blurry ones. However, the computational costs are high, and the restored sharp images are usually distorted. The effect of blurry image sequences to vSLAM system is analyzed, and the relationships between feature matching and motion blur are acquired to deal with the above mentioned problems. A local residual motion blur discrimination network is proposed to detect images with motion blur efficiently. Motion blur recognition results are coupled with a vSLAM system so that the feature extraction process is guided by the results from the local residual motion blur discrimination network. The performance of the vSLAM system can be effectively enhanced when it is applied to sequences with motion blur. Experimental results on the Technische Universität München dataset show that the proposed algorithm increases the average tracking length by about 200 frames compared with the original method on some image sequences with violent motions. This algorithm effectively improves the stability and accuracy of the vSLAM system.

A New Analytical Method for Link Slab Analysis

Yufan Luo,Quansheng Yan,Xiaogang Yue,Buyu Jia,Xiaolin Yu,Yuxuan Chen 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

Link slabs have been widely used to reduce the number of expansion joints. However, traditional reinforced concrete link slabs are prone to cracking issues, making it crucial to accurately calculate their internal forces. Currently, the most advanced link slab analysis methods treat the link slab and adjacent span as a rotational spring to simulate their restriction on the girder end rotation. However, this method does not comprehensively consider the link slab's influence, leading to conservative results. To address this issue, this study further considers the influence of girder end deformations on adjacent span, resulting in more accurate calculations of the link slab's internal forces. Additionally, the concept of the girder end rotation reduction coefficient is put forward for the first time, which provides a clearer and more quantified understanding of the link slab's influence. The proposed method is verified by a model test and a site test, which shows a significant improvement in accuracy compared to existing analytical methods. On this basis, a parametric study is conducted to investigate the influence of various parameters on the girder end rotation reduction coefficient, which indicates that span length, girder spacing, and support configuration have the greatest influence.

A Novel Hyperspectral Microscopic Imaging System for Evaluating Fresh Degree of Pork

Yi Xu,Quansheng Chen,Yan Liu,Xin Sun,Qiping Huang,Qin Ouyang,Jiewen Zhao 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.2

This study proposed a rapid microscopic examination method for pork freshness evaluation by using the self-assembled hyperspectral microscopic imaging (HMI) system with the help of feature extraction algorithm and pattern recognition methods. Pork samples were stored for different days ranging from 0 to 5 days and the freshness of samples was divided into three levels which were determined by total volatile basic nitrogen (TVB-N) content. Meanwhile, hyperspectral microscopic images of samples were acquired by HMI system and processed by the following steps for the further analysis. Firstly, characteristic hyperspectral microscopic images were extracted by using principal component analysis (PCA) and then texture features were selected based on the gray level co-occurrence matrix (GLCM). Next, features data were reduced dimensionality by fisher discriminant analysis (FDA) for further building classification model. Finally, compared with linear discriminant analysis (LDA) model and support vector machine (SVM) model, good back propagation artificial neural network (BP-ANN) model obtained the best freshness classification with a 100 % accuracy rating based on the extracted data. The results confirm that the fabricated HMI system combined with multivariate algorithms has ability to evaluate the fresh degree of pork accurately in the microscopic level, which plays an important role in animal food quality control.

A novel role of the cotton calcium sensor CBL3 was involved in Verticillium wilt resistance in cotton

Gao Shengqi,Hao Xiaoyan,Chen Guo,Hu Wenran,Zhao Zhun,Shao Wukui,Li Jianping,Huang Quansheng 한국유전학회 2024 Genes & Genomics Vol.46 No.8

Background Verticillium wilt, causes mainly by the soilborne pathogen Verticillium dahliae, is a devastated vascular disease resulting in huge financial losses in cotton, so research on improving V. dahliae stress tolerance in cotton is the utmost importance. Calcium as the second messenger acts as a crucial role in plant innate immunity. Cytosolic Ca2+during the pathogen infection is a significant increase in plant immune responses. Calcineurin B-like (CBL) proteins are widely known calcium sensors that regulate abiotic stress responses. However, the role of cotton CBLs in response to V. dahliae stress remains unclear. Objective To discover and utilize the gene to Verticillium wilt resistance and defense response mechanism of cotton. Methods Through screening the gene to Verticillium wilt resistance in cotton, four GhCBL3 copies were obtained from the current common cotton genome sequences. The protein domain and phylogenetic analyses of GhCBL3 were performed using NCBI Blast, DNAMAN, and MotifScan programs. Real-time RT-PCR was used to detect the expression of GhCBL3 gene in cotton seedlings under various stress treatments. The expression construct including GhCBL3 cDNA was transduced into Agrobacterium tumefaciens (GV3101) by heat shock method and transformed into cotton plants by Virus-Induced Gene Silencing (VIGS) method. The results of silencing of GhCBl3 on ROS accumulation and plant disease resistance in cotton plants were assessed. Results A member of calcineurin B-like proteins (defined as GhCBL3) in cotton was obtained. The expression of GhCBL3 was significantly induced and raised by various stressors, including dahliae, jasmonic acid (JA) and H2O2 stresses. Knockdown GhCBL3 in cotton by Virus-Induced Gene Silencing analysis enhanced Verticillium wilt tolerance and changed the occurrence of reactive oxygen species. Some disease-resistant genes were increased in GhCBL3-silencing cotton lines. Conclusion GhCBL3 may function on regulating the Verticillium dahliae stress response of plants. Background Verticillium wilt, causes mainly by the soilborne pathogen Verticillium dahliae, is a devastated vascular disease resulting in huge financial losses in cotton, so research on improving V. dahliae stress tolerance in cotton is the utmost importance. Calcium as the second messenger acts as a crucial role in plant innate immunity. Cytosolic Ca2+during the pathogen infection is a significant increase in plant immune responses. Calcineurin B-like (CBL) proteins are widely known calcium sensors that regulate abiotic stress responses. However, the role of cotton CBLs in response to V. dahliae stress remains unclear. Objective To discover and utilize the gene to Verticillium wilt resistance and defense response mechanism of cotton. Methods Through screening the gene to Verticillium wilt resistance in cotton, four GhCBL3 copies were obtained from the current common cotton genome sequences. The protein domain and phylogenetic analyses of GhCBL3 were performed using NCBI Blast, DNAMAN, and MotifScan programs. Real-time RT-PCR was used to detect the expression of GhCBL3 gene in cotton seedlings under various stress treatments. The expression construct including GhCBL3 cDNA was transduced into Agrobacterium tumefaciens (GV3101) by heat shock method and transformed into cotton plants by Virus-Induced Gene Silencing (VIGS) method. The results of silencing of GhCBl3 on ROS accumulation and plant disease resistance in cotton plants were assessed. Results A member of calcineurin B-like proteins (defined as GhCBL3) in cotton was obtained. The expression of GhCBL3 was significantly induced and raised by various stressors, including dahliae, jasmonic acid (JA) and H2O2 stresses. Knockdown GhCBL3 in cotton by Virus-Induced Gene Silencing analysis enhanced Verticillium wilt tolerance and changed the occurrence of reactive oxygen species. Some disease-resistant genes were increased in GhCBL3-silencing cotton lines. Conclusion GhCBL3 may function on regulating the Verticillium dahliae stress response of plants.

Prediction of black tea fermentation quality indices using NIRS and nonlinear tools

Chunwang Dong,Hongkai Zhu,Jinjin Wang,Haibo Yuan,Jiewen Zhao,Quansheng Chen 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.4

Catechin content, the ratio of tea polyphenols and free amino acids (TP/FAA), as well as the ratio of theaflavins and thearubigins (TFs/TRs) are important biochemical indicators to evaluate fermentation quality. To achieve rapid determination of such biochemical indicators, synergy interval partial least square and extreme learning machine combined with an adaptive boosting algorithm, Si-ELM-AdaBoost algorithm, were used to establish quantitative analysis models between near infrared spectroscopy (NIRS) and catechin content and between TFs/TRs and TP/FAA, respectively. The results showed that prediction performance of the Si-ELM-AdaBoost mixed algorithm is superior than that of other models. The prediction results with root-mean-square error of prediction ranged from 0.006 to 0.563, the ratio performance deviation values exceeded 2.5, and predictive correlation coefficient values exceeded 0.9 in the prediction model of each biochemical indicator. NIRS combined with Si-ELMAdaBoost mixed algorithm could be utilized for online monitoring of black tea fermentation. Meanwhile, the AdaBoost algorithm effectively improved the accuracy of the ELM model and could better approach the nonlinear continuous function.

Discrimination of Chinese Baijiu grades based on colorimetric sensor arrays

Hao Lin,Wen-cui Kang,Hong-juan Jin,Zhong-xiu Man,Quansheng Chen 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.8

In this study, a novel colorimetric sensor arraybased on chemo dyes including porphyrins and pH indicatorswere developed to analyse the volatile organiccompounds of Chinese Baijiu with different grades. Ethylacetate, ethyl butyrate and ethyl caproate appeared bysignificantly different concentration in different Baijiugrades measuring by gas chromatography and mass spectrometryand they were chosen as characteristic volatileorganic components. The olfactory visualization systembased on colorimetric sensor arrays was used to identifydifferent Baijiu grades. The data were processed bybuilding the principle components analysis, linear discriminantanalysis and K-nearest neighbor classificationmodels with the results of sensory evaluation and olfactoryvisualization system. This work presents a new-style colorimetricsensor using sensitive chemo dyes which hassignificant potential in quantitative analysis of volatileorganic compounds, afterwards identifying different gradesof Baijiu.

The Cancer/Testis Antigen CT45A1 Promotes Transcription of Oncogenic Sulfatase-2 Gene in Breast Cancer Cells and Is Sensible Targets for Cancer Therapy

Ping Yang,Yingnan Qiao,Huaidong Liao,Yizheng Huang,Mei Meng,Yu Chen,Quansheng Zhou 한국유방암학회 2023 Journal of breast cancer Vol.26 No.2

Purpose: Invasive breast carcinomas (BRCAs) are highly lethal. The molecular mechanisms underlying progression of invasive BRCAs are unclear, and effective therapies are highly desired. The cancer-testis antigen CT45A1 promotes overexpression of pro-metastatic sulfatase-2 (SULF2) and breast cancer metastasis to the lungs, but its mechanisms are largely unknown. In this study, we aimed to elucidate the mechanism of CT45A1-induced SULF2 overexpression and provide evidence for targeting CT45A1 and SULF2 for breast cancer therapy. Methods: The effect of CT45A1 on SULF2 expression was assessed using reverse transcription polymerase chain reaction and western blot. The mechanism of CT45A1-induced SULF2 gene transcription was studied using protein-DNA binding assay and a luciferase activity reporter system. The interaction between CT45A1 and SP1 proteins was assessed using immunoprecipitation and western blot. Additionally, the suppression of breast cancer cell motility by SP1 and SULF2 inhibitors was measured using cell migration and invasion assays. Results: CT45A1 and SULF2 are aberrantly overexpressed in patients with BRCA; importantly, overexpression of CT45A1 is closely associated with poor prognosis. Mechanistically, gene promoter demethylation results in overexpression of both CT45A1 and SULF2. CT45A1 binds directly to the core sequence GCCCCC in the promoter region of SULF2 gene and activates the promoter. Additionally, CT45A1 interacts with the oncogenic master transcription factor SP1 to drive SULF2 gene transcription. Interestingly, SP1 and SULF2 inhibitors suppress breast cancer cell migration, invasion, and tumorigenicity. Conclusion: Overexpression of CT45A1 is associated with poor prognosis in patients with BRCA. CT45A1 promotes SULF2 overexpression by activating the promoter and interacting with SP1. Additionally, SP1 and SULF2 inhibitors suppress breast cancer cell migration, invasion, and tumorigenesis. Our findings provide new insight into the mechanisms of breast cancer metastasis and highlight CT45A1 and SULF2 as sensible targets for developing novel therapeutics against metastatic breast cancer.

SsPsaH, a H subunit of the photosystem I reaction center of Suaeda salsa, confers the capacity of osmotic adjustment in tobacco

Xiaoyan Hao,Jianping Li,Shengqi Gao,Zumuremu Tuerxun,Xiaochun Chang,Wenran Hu,Guo Chen,Quansheng Huang 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Abiotic stress effects agricultural production, so research on improving stress tolerance of crop is important. Suaeda salsa is a halophyte with high salt and drought tolerance and ability to desalinate saline soil and improve soil quality. Objective: To discover and utilize of salt and drought tolerance-related genes, we further investigated the mechanisms of salt and drought tolerance. Methods: Through screening a salt treated Suaeda salsa cDNA library and further cloning a H subunit of the photosystem I reaction center SsPsaH cDNA, and then the protein domain and phylogenetic analyses of PSI genes was conducted with the NCBI Blast, DNAMAN, and MotifScan programs. The S. salsa seedlings were subjected to various stress treatments and analyze expression of SsPsaH under these treatments by real-time RT-PCR. SsPsaH expression construct was introduced into S. pombe cells by electroporation and transformed into N. tabacum plants by the leaf disc transformation method. Results: A member of the H subunit of the Photosystem I reaction center (defined as SsPsaH) was obtained. The expression of SsPsaH was up-regulated by abscisic acid (ABA), salt, and drought stress treatments. Over-expressing SsPsaH in recombinant yeasts enhanced high salinity tolerance and increased tolerance to sorbitol during seed germination and seedling root development in tobacco, respectively. Some stress-related mark genes such as a LEA family gene of NtLEA, a binding protein of a drought response element of NtDREB, the ascorbate peroxidase gene (NtAPX) were also up-regulated in SsPsaH overexpressing transgenic tobacco lines. Conclusions: These results show that SsPsaH may contribute to the salt and osmotic stress response of plants.