Effect of Multi-Level Microstructure on Local and Bulk Mechanical Properties in Micro-Injection Molded PC/PET Blend

Jianping Ren,Jing Jiang,Zihui Li,Jianhua Hou,Qian Li 한국고분자학회 2020 Macromolecular Research Vol.28 No.10

This study introduces a method to investigate the relationship between the multi-level microstructures and mechanical properties of polymer blends prepared by micro-injection molding (μIM). Special morphological features were systematically researched. Polycarbonate (PC), poly(ethylene terephthalate) (PET), and PC/PET microparts all exhibit typical “skin-core” morphologies. The thickness of the core layer is much greater than that of the skin layer, and the thickness of the skin layer gradually decreases along the flow direction. Photoacoustic Fourier transform infrared spectroscopy records reveal that the PC molecular chain has the biggest orientation degree, followed by PC/PET and PET chains under the same μIM processing conditions. Moreover, the molecular chains orientation in the skin layer is more than 50% that in the core layer. Nanoindentation tests are conducted to study local mechanical properties. The higher modulus in the shear layer is affected to a greater extent by high shear action in comparison with the frozen and core layers. Uniaxial tensile testing demonstrates that the tensile strength of PC/PET micropart is 15.5% higher than that of the PET micropart, while the toughness is 16% higher than that of the PC microparts. In-situ, high- speed tensile imaging, combined with scanning electron microscopy micrographs of the fracture section, are used to study the fracture behaviors of the microparts. The results gathered in this paper may provide a theoretical basis and data to support the feasibility and efficiency of micro-injection molded polymer blends.

Transcriptome sequencing and characterization of Astragalus membranaceus var. mongholicus root reveals key genes involved in flavonoids biosynthesis

Jianping Liang,Wenqian Li,Xiaoyun Jia,Ying Zhang,Jianping Zhao 한국유전학회 2020 Genes & Genomics Vol.42 No.8

Background Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao is a traditional medicinal herb of Leguminosae since it contains bioactive compounds such as flavonoids, which have significant pharmacological effects on immunity and antioxidant. However, the scanty genomic and transcriptome resources of Astragalus membranaceus have hindered further exploration of its biosynthesis and accumulation mechanism. Objective This project aim to further improve our understanding of the relationship between transcriptional behavior and flavonoids content of A. mongholicus. Methods The accumulation of flavonoids and related gene expression in five different developmental stages (A: vegetative, B: florescence, C: fruiting, D: fruit ripening and E: defoliating stages) of A. mongholicus root were studied by combining UV spectrophotometry and transcriptomic techniques. The de novo assembly, annotation and functional evaluation of the contigs were performed with bioinformatics tools. Results After screening and assembling the raw data, there were a total of 158,123 unigenes with an average length of 644.89 bp were finally obtained, which has 8362 unigenes could be jointly annotated by NR, SwissProt, eggNOG, GO, KEGG and Pfam databases. KEGG enrichment analysis was performed on differentially expressed genes(DEGs)in the four groups (A vs. B, B vs. C, C vs. D, D vs. E). The results showed that many DEGs in each group were significantly enriched to flavonoids biosynthesis related pathways. Among them, a number of 86 were involved in the biosynthesis of isoflavonoid (12), flavonoid (5) and phenylpropanoid (69). Further analysis of these DEGs revealed that the expression levels of key genes such as PAL, 4CL, CCR , COMT, DFR, etc. were all down-regulated at the fruiting stage, and then raised at the fruit ripening stage. This expression pattern was similar to the accumulation trend of total flavonoids content. Conclusions In summary, this comprehensive transcriptome dataset allowed the identification of genes associated with flavonoids metabolic pathways. The results laid a foundation for the biosynthesis and regulation of flavonoids. It also provided a scientific basis for the most suitable harvest time and resource utilization of A. mongholicus.

Silencing of Long Non-Coding RNA MALAT1 Promotes Apoptosis of Glioma Cells

Jianping Xiang,Shifeng Guo,Shuling Jiang,Yuelong Xu,Jiwei Li,Li Li,Jinyu Xiang 대한의학회 2016 Journal of Korean medical science Vol.31 No.5

The metastasis-associated lung adenocarcinoma transcription 1 (MALAT1) is a highly conserved long non-coding RNA (lncRNA) gene. However, little is known about the pathological role of lncRNA MALAT1 in glioma. In the present study, we explored the expression level of lncRNA MALAT1 in primary glioma tissues as well as in U87 and U251 glioma cell lines. Using qRT-PCR, we found that the expression of lncRNA MALAT1 was significantly increased in glioma tissues compared with that of paracancerous tissues. Meanwhile, the expression of MALAT1 was highly expressed in U98 and U251 cells. In order to explore the function of MALAT1, the expression of MALAT1 was greatly reduced in U87 and U251 cells transfected with siRNA specifically targeting MALAT1. Consequently, cell viability of U87 and U251 cells were drastically decreased after the knockdown of MALAT1. Concomitantly, the apoptosis rate of the two cell lines was dramatically increased. Furthermore, the expression levels of some tumor markers were reduced after the knockdown of MALAT1, such as CCND1 and MYC. In summary, the current study indicated a promoting role of MALAT1 in the development of glioma cell.

The influence of the extent of lymph node metastasis on the prognosis for patients with intrahepatic cholangiocarcinoma

Jianping Wang*,Man Shu*,Hong Peng,Shaoqiang Li,Dongming Li,Jingxian Shen,Ming Kuang,Ying Zhang,Zebin Chen 대한외과학회 2023 Annals of Surgical Treatment and Research(ASRT) Vol.104 No.5

Purpose: Reports showed that some of intrahepatic cholangiocarcinoma (ICC) patients with lymph node metastasis (LNM) may also gain survival benefit undergone resection. However, the effect of the extent of LNM on prognosis and surgical indication is barely discussed. Methods: From September 1994 to November 2018, primary ICC patients undergone initial curable surgery were enrolled. Based on the extent of LNM, we divided these patients into 4 groups, including patients with no LNM (group N0), LNM to hepatoduodenal ligament or common hepatic artery (region A, group A), LNM to gastrohepatic lymph nodes for left liver ICC and periduodenal and peripancreatic lymph node for right liver ICC (region B, group B), or LNM beyond these regions (region C, group C). Multivariable Cox regression analysis was performed to identify the prognostic factors for recurrence- free survival (RFS) and overall survival (OS) in all groups. Results: A total of 133 patients were enrolled. There were 56, 21, 17, and 39 patients in groups N0, A, B, and C, respectively. There was significant difference between groups N0 and C in RFS (P < 0.001) and OS (P = 0.002). When we compared group N0 + A + B with group C, we also found that RFS (P < 0.001) and OS (P = 0.007) were significantly different. In multivariable analysis, the extent of LNM was an independent risk factor for RFS (P < 0.050). Conclusion: ICC patients with the LNM to regions A and B could still achieve good prognosis with resection. Surgery should be carefully considered when LNM to region C.

애니메이션의 확대

이건평(Li Jianping) 한국애니메이션학회 2011 한국애니메이션학회 학술대회지 Vol.2011 No.10

Effect of a cotton intercrop on spider mite populations in jujube trees

Haiqiang Li,Qian Li,Dongmei Wang,Jian Liu,Jianping Zhang,Yan-Hui Lu 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1

In the arid and semi-arid parts of Xinjiang (China), jujube (Zizyphus jujube Mill) orchards are regularly intercropped with cotton. In 2016–2017, a field study was conducted to compare the species composition and infestation pressure of spider mites in local jujube trees under a cotton-jujube intercropping system and a jujube monocrop system. Our results showed that Tetranychus truncatus Ehara accounted for 94.2–98.5% of all spider mites in either cropping systems. Overall abundance of tree-inhabiting spider mites, including T. truncatus, in intercropped orchards was generally higher than in monocrop ones. Combining our work with earlier findings, we demonstrate how intercropping of cotton and jujube trees generally increases population levels of spider mites on either crop.

Fuzzy system and Improved APIT (FIAPIT) combined range-free localization method for WSN

( Xiaofeng Li ),( Liangfeng Chen ),( Jianping Wang ),( Zhong Chu ),( Qiyue Li ),( Wei Sun ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.7

Among numerous localization schemes proposed specifically for Wireless Sensor Network (WSN), the range-free localization algorithms based on the received signal strength indication (RSSI) have attracted considerable research interest for their simplicity and low cost. As a typical range-free algorithm, Approximate Point In Triangulation test (APIT) suffers from significant estimation errors due to its theoretical defects and RSSI inaccuracy. To address these problems, a novel localization method called FIAPIT, which is a combination of an improved APIT (IAPIT) and a fuzzy logic system, is proposed. The proposed IAPIT addresses the theoretical defects of APIT in near (it`s defined as a point adjacent to a sensor is closer to three vertexes of a triangle area where the sensor resides simultaneously) and far (the opposite case of the near case) cases partly. To compensate for negative effects of RSSI inaccuracy, a fuzzy system, whose logic inference is based on IAPIT, is applied. Finally, the sensor`s coordinates are estimated as the weighted average of centers of gravity (COGs) of triangles` intersection areas. Each COG has a different weight inferred by FIAPIT. Numerical simulations were performed to compare four algorithms with varying system parameters. The results show that IAPIT corrects the defects of APIT when adjacent nodes are enough, and FIAPIT is better than others when RSSI is inaccuracy.

Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway

Jianxiong Li,Bin Li,Yujie Bu,Hailin Zhang,Jia Guo,Jianping Hu,Yanfang Zhang 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.4

SERTA domain-containing protein 1 (Sertad1) is upregulated in the models of DNA damage and Alzheimer’s disease, contributing to neuronal death. However, the role and mechanism of Sertad1 in ischemic/hypoxic neurological injury remain unclear. In the present study, our results showed that the expression of Sertad1 was upregulated in a mouse middle cerebral artery occlusion and reperfusion model and in HT22 cells after oxygen-glucose deprivation/reoxygenation (OGD/R). Sertad1 knockdown significantly ameliorated ischemia-induced brain infarct volume, neurological deficits and neuronal apoptosis. In addition, it significantly ameliorated the OGD/R-induced inhibition of cell viability and apoptotic cell death in HT22 cells. Sertad1 knockdown significantly inhibited the ischemic/hypoxic-induced expression of p-Rb, B-Myb, and Bim in vivo and in vitro. However, Sertad1 overexpression significantly exacerbated the OGD/R-induced inhibition of cell viability and apoptotic cell death and p-Rb, B-Myb, and Bim expression in HT22 cells. In further studies, we demonstrated that Sertad1 directly binds to CDK4 and the CDK4 inhibitor ON123300 restores the effects of Sertad1 overexpression on OGD/R-induced apoptotic cell death and p-Rb, B-Myb, and Bim expression in HT22 cells. These results suggested that Sertad1 contributed to ischemic/hypoxic neurological injury by activating the CDK4/p-Rb pathway.

北京城的持續与變動

李建平(LI Jianping) 서울시립대학교 서울학연구소 2016 서울학연구 Vol.- No.64

베이징 옛 도성에는 8킬로미터나 되는 긴 중축선(中軸線)이 있는데, 여기에 자리한 건축들은 중국의 역사와 문화를 잘 보여준다. 옛 도성이었던 베이징에는 주로 성벽과 성문 · 골목[胡同] · 사합원(四合院)과 점포들이 있었다. 건물의 색조는 대부분 회색 벽에 회색 기와였다. 또 다른 중요한 점은 베이징 옛 도성의 남북을 잇고 있다는 것이다. 남쪽으로 영정문(永定門)에서 시작하고 천교(天橋)를 거쳐, 정양문(正陽門) · 대청문(大淸門) · 천안문(天安門) · 단문(端門) · 오문(午門)을 거쳐 황제가 있는 금란보전(金鑾寶殿)인 태화전(太和殿)에 이어지며 중화전(中和殿) · 보화전(保和殿)을 지나 황제와 황후의 침전인 건청궁(乾淸宮)·교태전(交泰殿) · 곤녕궁(坤寧宮)으로 이어지며 어화원(禦花園)과 종교건물인 흠안전(欽安殿)으로 이어진다. 황궁의 후문인 신무문(神武門) · 경산(景山)의 만춘정(萬春亭) · 황성의 후문인 지안문(地安門), 그리고 만녕교(萬寧橋) · 고루(鼓樓) · 종루(鍾樓)까지 이어진다. 전체 길이가 7.8 킬로미터나 되는 중축선에는 붉은 벽에 누런 기와로 꾸며진 높고 큰 건물들이 들어서 도심을 장식하여 베이징 옛 도성의 풍모와 특징을 선명하게 보여주고 있다. 베이징 옛 도성의 도시의 등뼈로 일컬어지는 중축선에는 도시의 정기와 신력(神力)이 숨어 있는 도시의 용맥(龍脈)이라고 할 수 있다. 원(元) · 명(明) · 청(淸) 삼대에 거쳐 베이징 옛 도성의 중축선은 계획에서 건설에 이르기까지 끊임없이 확충되고 다듬어지면서 황제의 도성을 상징하는 지금의 모습으로 거듭났다. 그러나 19세기 말 20세기 초에 이 중축선과 베이징 도성은 전란을 겪으면서 파손되었고 심지어는 불타버리는 재앙을 맞이하였다. 지금의 모습은 재건한 이후의 모습니다.

A Resting-state Functional Magnetic Resonance Imaging Study of Whole-brain Functional Connectivity of Voxel Levels in Patients With Irritable Bowel Syndrome With Depressive Symptoms

( Jie Li ),( Ping He ),( Xingqi Lu ),( Yun Guo ),( Min Liu ),( Guoxiong Li ),( Jianping Ding ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2021 Journal of Neurogastroenterology and Motility (JNM Vol.27 No.2

Background/Aims Depressive symptom is one of the most common symptoms in patients with irritable bowel syndrome (IBS), but its pathogenetic mechanisms remain unclear. As a voxel-level graph theory analysis method, degree centrality (DC) can provide a new perspective for exploring the abnormalities of whole-brain functional network of IBS with depressive symptoms (DEP-IBS). Methods DC, voxel-wise image and clinical symptoms correlation and seed-based functional connectivity (FC) analyses were performed in 28 DEP-IBS patients, 21 IBS without depressive symptoms (nDEP-IBS) patients and 36 matched healthy controls (HC) to reveal the abnormalities of whole brain FC in DEP-IBS. Results Compared to nDEP-IBS patients and HC, DEP-IBS patients showed significant decrease of DC in the left insula and increase of DC in the left precentral gyrus. The DC’s z-scores of the left insula negatively correlated with depression severity in DEP-IBS patients. Compared to nDEP-IBS patients, DEP-IBS patients showed increased left insula-related FC in the left inferior parietal lobule and right inferior occipital gyrus, and decreased left insula-related FC in the left precentral gyrus, right supplementary motor area (SMA), and postcentral gyrus. In DEP-IBS patients, abstracted clusters’ mean FC in the right SMA negatively correlated with depressive symptoms. Conclusions DEP-IBS patients have abnormal FC in brain regions associated with the fronto-limbic and sensorimotor networks, especially insula and SMA, which explains the vicious circle between negative emotion and gastrointestinal symptoms in IBS. Identification of such alterations may facilitate earlier and more accurate diagnosis of depression in IBS, and development of effective treatment strategies. (J Neurogastroenterol Motil 2021;27:248-256)