Promoter Analysis of Bombyx mori Nucleopolyhedrovirus Ubiquitin Gene

Xu’ai Lin,Yin Chen,Yongzhu Yi,Jie Yan,Zhifang Zhang 한국미생물학회 2008 The journal of microbiology Vol.46 No.4

The aim of this study was to analyze the characteristics of Bombyx mori nucleopolyhedrovirus (BmNPV) ubiquitin gene promoter and the effects of conserved motifs, such as TAAG, TATA, and CAAT, along with baculovirus enhancer homologous region 3 (hr3), on promoter activity. Ubiquitin gene of BmNPV was expressed during the late phase of virus infection. In the presence of viral factors, significant reduction of promoter activity was observed by deletion of -382 to -124 bp upstream of ATG. The fragment between -187 and -383 bp upstream of ATG, including distal TAAG, CAAT motif, and TATA box, could also drive expression of the reporter gene. The mutation of cis-elements TATA boxes and TAAG motifs significantly decreased the promoter’s activity, while CAAT mutations enhanced promoter activity by 2- or 3-fold, as compared with the native promoter. In the presence of BmNPV, hr3, both located downstream of the reporter gene of the same vector, and separate vector, could significantly enhance transcription activity of ubiquitin promoter as compared to the control. We concluded that BmNPV ubiquitin gene might be regulated by dual sets of promoter elements, where TAAG and TATA box may positively regulate the expression of ubiquitin, while CAAT motif functions as a negative regulator. Viral factor(s) play an important role in the co-activation of hr3 and promoter.

Cloning, Expression, and Functional Characterization of the Dunaliella salina 5-enolpyruvylshikimate-3-phosphate Synthase Gene in Escherichia coli

Yi, Yi,Qiao, Dairong,Bai, Linhan,Xu, Hui,Li, Ya,Wang, Xiaolin,Cao, Yi The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.2

5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.

Five new anthraquinones from the seed of Cassia obtusifolia

Yi-Long Xu,Li-Ying Tang,Xi-Dan Zhou,Guo-Hong Zhou,Zhu-Ju Wang 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

A phytochemical investigation on the seeds ofCassia obtusifolia led to the isolation of five new anthraquinones,including one aglycon and four glycosides. Thestructures were elucidated by analysis of extensive spectroscopicdata and the results of acid hydrolysis. All theseisolates were evaluated for their inhibitory effects againsta-glucosidase, and 1 showed potent activity with IC50value of 185 ± 15 lM.

十八世紀朝淸文士筆談內容考述

( Yi Xu ),( Li Chen ) 한국외국어대학교 중국연구소 2014 中國硏究 Vol.60 No.-

The 18th century is a period when Qing and Choson literati communicate frequently with a variety of communicating forms such as conversations by writing, the exchange of poetry, reciprocating letters, asking for providing preface and postscript, literary criticism as well as giving books as presents, among which the conversations by writing is the most popular way of interaction. According to statistical study by the author, at least 193 detailed conversations by writing happened in the 18th century were recorded in the extant Yeonhaeng Rok, one of Chinese and Korean ancient scriptures. Its contents mainly covered ten aspects, in which some in-depth exchange was recorded. Therefore, these materials are of great literature value. Regarding to the communication of etiquette system and custom, Qing people`s comments on Choson ambassadors` clothing and inturn Choson views of binding feet of Qing women are always the key points. Besides, it also includes the communication of imperial examination system in Qing Dynasty and Ancient Korea;the discussion about academy and literary between Qing and Choson scholars; Choson literati` concerns with religion; Choson literati` focus on the Ming historical events, Qing political and agricultural situation as well associal development; Choson literati` acquaintance with compilation of books in Qing Dynasty, for example "The Imperial Collection of Four Treasures";the exchange of banned books in Qing Dynasty between Qing and Choson literati; Choson scholars` care for the landscape, geography of China and places of interest in China. Qing people also pay attention to Choson in many regards, for instance, landscape and geographical situation, scene style and feature views, ancient authors and their works, history, etiquette, custom, education, languageetc. It is obvious to find the diversity of their conversation topics. And these topics are closely associated with the culture, custom, history and the focus on Confucianism of Qing Dynasty and Choson. It should also be emphasized that no matter how abundant the conversations are, the key point is always related to concerns of Choson literati when visiting Qing Dynasty and their own scholar traits.

朝鮮使者眼中的淸代戱曲

( Yi Xu ) 연민학회 2010 연민학지 Vol.14 No.-

본고는 조선시기 『燕行錄』중의 희곡 관련 자료를 연구대상으로 하여, 이국자의 시각으로 본 청대 희곡의 연극 활동 상황을 고찰하여 본다. 본고는 우선 『燕行錄』중에 청대 희곡의 연출 환경에 대한 묘사를 검토 분석하고, 그 다음 희곡 연출 복식에 대한 조선 사신들의 관심에 대해 논술하고, 마지막으로 『燕行錄』에 기록된 희곡 극목에 근거하여 청대 민간 희곡과 궁정 희곡의 분야를 서술해보려고 한다. 이런 보기드문 희곡 자료에 대한 정리를 통해 청대 희곡 연구의 시야를 넓혀보려고 한다. The research object of this paper is the Chinese Opera materials that are contained in YanXingLu (Travelogues of Korean Envoys to China). The activities of the Chinese opera are depicted through the perspective of Li Dynasty`s scholars. Firstly, I will analyze the environment of the performance recorded by the Korean envoys. Secondly, I will explain the reason that Korean scholars pay attention to the costume of the Chinese Opera. Finally, I will illustrate the differences between the folk drama and the palace opera. We could expand our research sight on the Qing Dynasty Opera by analyzing these ancient art works.

Framing advocacy event: Comparing news coverage and Facebook comments of the Belt and Road Forum in Pakistan and the USA

Xu, Yi World Association for Triple Helix and Future Stra 2021 Journal of Contemporary Eastern Asia Vol.20 No.1

With regard to the recent developments in public diplomacy, the increasing fusion of strategic communication appears necessary. China engages in public diplomacy with a strategic purpose to shape its national image abroad. Hosting diplomatic advocacy event is regarded as an instrument with expectations to present reliable and responsible image and promote international collaborations. The present research focuses on the Belt and Road Forum (BRF) in May 2017 with the objective to analyze its outcomes and influence on the international news agenda, news frames, and foreign citizens' comments online. The quantitative content analyses are used to compare the media reports (N=364) and Facebook users' comments on the selected news (N=957) between the US and Pakistan. Results reveal that Pakistani media provided more diverse frames and attributed more positive evaluations to the BRF than the US media. However, Facebook comments expressed more unfavorable opinions toward the BRF and China's image with rare differences between two countries. In conclusion, the BRF has served as an eye-catching advocacy of Chinese foreign policy, as it influenced the news agenda in two selected countries. However, news frames vary due to the differences in media system and the involvement in the BRF. China's public diplomacy practices follow a traditional top-down communication which needs meticulous subdivision of target stakeholders, delicate messaging strategies, and integrated tactics.

The Influence of Cultural Intelligence and Institutional Distance of Chinese and Korean Contractors on the Performance of International Construction Projects

Yi-Hsin Lin,Huijin Zhang,김찬중,Zhao Xu,Shuangshuang Xu 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.9

Cultural intelligence can effectively solve cross-cultural problems, which encourages contractors to adapt to the new international market environment. However, in international projects, the effectiveness of cultural intelligence can be complicated or moderated by the host country's institutional environment. This study explores the impact of cultural intelligence on the performance of international construction projects and the moderating effect of institutional distance, then compares the differences between Chinese contractors and Korean contractors. In this paper, eleven hypotheses were proposed, and an empirical analysis was conducted to test the hypotheses and measure the impact of cultural intelligence on the performance of international construction projects and the moderating effect of institutional distance. The results from 144 valid questionnaires indicated that process capability and path capability in cultural intelligence have a significant positive impact on the performance of international construction projects, while formal institutional distance can positively moderate the relationship between position capability and international construction project performance. Moreover, Chinese and Korean contractors display significant differences in the moderating role of institutional distance. These findings provide theoretical support and strategic suggestions for not only contractors in China and Korea but for all international contractors seeking to improve project performance through cultural intelligence and institutional distance.

Albumin-to-Fibrinogen Ratio as an Independent Prognostic Parameter in Untreated Chronic Lymphocytic Leukemia: A Retrospective Study of 191 Cases

Yi-Xin Zou,Jia Qiao,Hua-Yuan Zhu,Rui-Nan Lu,Yi Xia,Lei Cao,Wei Wu,Hui Jin,Wen-Jie Liu,Jin-Hua Liang,Jia-Zhu Wu,Li Wang,Lei Fan,Wei Xu,Jian-Yong Li 대한암학회 2019 Cancer Research and Treatment Vol.51 No.2

Purpose Chronic lymphocytic leukemia (CLL) is one of the most frequent type of B-cell chronic lymphoproliferative disorders and chronic inflammation takes part in the development of CLL. However, there has been no valid immune biomarker to predict the prognosis of untreated CLL patients. Materials and Methods In this retrospective study, we analyzed the clinical correlations and prognostic value of albumin-to-fibrinogen ratio (AFR) detected at diagnosis in 191 CLL patients. Results The cut-off value of AFR was 9.7 calculated by X-tile. Patients who were more than 65 years old were often accompanied by low level of AFR (p < 0.001). Survival analysis showed that patients with low level of AFR had shorter overall survival (OS) than patients with high level of AFR (p < 0.001). Multivariate analysis illustrated that AFR had a negative impact on OS (p=0.003) and was independent of parameters involved in CLL international prognostic index and other prognostic markers such as CD38 and ZAP-70. Conclusion These data provide a comprehensive view of AFR and shows that AFR at diagnosis is an adverse prognostic factor in untreated CLL patients.

Humic Substances Act as Electron Acceptor and Redox Mediator for Microbial Dissimilatory Azoreduction by Shewanella decolorationis S12

( Yi Guo Hong ),( Guo Jun ),( Zhi Cheng Xu ),( Mei Ying Xu ),( Guo Ping Sun ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.3

A Comparison of Transradial and Transfemoral Percutaneous Coronary Intervention in Chinese Women Based on a Propensity Score Analysis

Yi Xu,Chen Jin,Shubin Qiao,Yongjian Wu,Hongbing Yan,Kefei Dou,Bo Xu,Jingang Yang,Yuejin Yang 대한심장학회 2018 Korean Circulation Journal Vol.48 No.8

Background and Objectives: Over the past decades, transradial approach for percutaneous coronary intervention (PCI) has been increasingly adopted in clinical practice. Women represent a large population who will possibly benefit from PCI, but they are often under-represented in clinical studies. Therefore, the role of TRI in women remains to be further defined. This study sought to compare safety and efficacy for transradial intervention (TRI) and transfemoral intervention (TFI) in women undergoing PCI in China. Methods: The study population consisted of 5,067 women undergoing PCI at Fuwai Hospital, Beijing, China between 2006 and 2011 (TRI: n=4,105, TFI: n=962). Incidence rates of clinical outcomes during hospitalization and at 1-year follow-up were compared between TRI and TFI. In order to minimize potential biases, a 1:1 propensity score matching (PSM) was performed. A total of 899 pairs were matched. Results: Baseline and procedural characteristics were well-balanced between TRI and TFI groups after controlling for confounders using PSM. TRI was associated with reduced major post-PCI bleeding (odds ratio [OR], 0.64; 95% confidence interval [CI], 0.54–0.76; p<0.001) and access site complications (OR, 0.67; 95% CI, 0.61–0.74; p<0.001) after PSM. There was no statistical differences in the incidence rates of major adverse cardiac events (a composite of cardiac death, myocardial infarction, and target vessel revascularization) both during hospitalization and at 1-year follow-up (p>0.05). Conclusions: In this propensity score-based analysis of TRI versus TFI in Chinese women, TRI showed advantages of safety and feasibility over TFI. A wider adoption of TRI in women has the potential to improve outcomes in treatment of coronary artery diseases.