Genetic Characterization of Haemagglutinin 1 Domain of Influenza B Viruses Isolated in Korea during $1988{\sim}1999$

Shin, Gu-Choul,Na, Byoung-Kuk,Lee, Joo-Yeon,Ahn, Jung-Bae,Park, Jong-Won,Lee, Jin-Soo,Kim, Jee-Hee,Kim, Woo-Joo,Kang, Chun The Korean Society for Microbiology 2003 Journal of Bacteriology and Virology Vol.33 No.4

Although Korean influenza virus isolates have been genetically associated with the vaccine strains of the corresponding year, influenza B viruses have prevailed almost every year in Korea during the past decades. We have 'analyzed the genetic characteristics and evolutionary patterns of the haemagglutinin (HA) 1 domains of influenza B viruses isolated during 1988-1999 using direct RT-PCR and sequencing. Phylogenetic analysis of influenza B viruses isolated in Korea indicated that antigenically and genetically distinguishable strains of the lineage II and lineage III variants had been cocirculated. Variants prevailed in early 1990s are represented in 1996/97 and 1998/99 and some different variants have been co circulated geographically and prevailed concurrently in Korea. All HA1s of Korean isolates have amino acid substitutions mainly in the region between position 124 and 310, which was previously proposed an immunodominant region. Insertion-deletion patterns of the HA gene revealed that Korean influenza B viruses were evolved from Lee40 with different manner between lineage II and III viruses. Lineage III viruses were also divided into two groups as conserved group and inserted group, in relation to Lee40. But lineage II viruses had evolved with directional pattern. Antigenic index proposed that influenza B isolates prevailed since 1996/97 seasons might had emerged from the antigenic variants of a Seo1697-like virus and that new variants might appear from the lineage II viruses resulting in persistent prevalence in Korea.

본태성 고혈압쥐에서 Myogenic Tone의 변화:RhoA 단백질 및 Protein Kinase C의 역할

서정필,류성경,강나영,안덕선,이영호 대한순환기학회 2002 Korean Circulation Journal Vol.32 No.3

말기신부전 환자에서 혈액투석 전후의 QT간격분산 비교

강대웅,정지용,윤나라,안치용,김종오,신병철,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Background: Some cases of QT interval, corrected QT interval (QTc), QT dispersion (QTd) and corrected QT dispersion (QTcd) have been reported in ESRD, but these results are variable and the influence of a hemodialysis is still controversial, In this study, we investigated the effect of hemodialysis on QT and QTc interval and QT and QTc dispersion in patients with ESRD 15 min before and 15 minute after each hemodialysis. Methods: Sixty-seven patients with ESRD (men 33 & women 34) on three-times stable hemodialysis (>3 months) were randomly enrolled. Fifty control subjects with a similar age and normal renal function were enrolled from this hospital. Routine biochemical studies were measured pre- and post dialysis, at the time of the ECG. Plasma Na+, K+, BUN, creatinine, ionized calcium and phosphate were checked. Twelve-lead electrocardiographs were performed at 10㎜/mv and 50㎜/s using a HewIett-Packard Pagewriter 100, before and after a single hemodialysis session.The QT interval was measured from the onset of the QRS complex to the end of the T wave. If the end of the T wave was not clear in a particular lead then it was excluded from analysis. When U waves were present, the end of the T wave was taken as the nadir between the T and U waves. Each QT interval was corrected for heart rate using Bazett's formula (QTc==QT/√(RR)) (ms). The difference between maximal and minimal QT interval duration was defined as QT dispersion (QTd) in each of the 12 leads. Result: This study demonstrates that QT, QTd, QTcd is higher in hemodialysis patients compared with control subjects, and QT and QTd rise postdialysis to levels comparable to those seen acutely following myocardial infarction, when patients are at greatly increased risk of potentially fatal ventricular arrhythmias, Conclusions: QT interval and QT dispersion, markers of risk for arrhythmias and sudden death, are elevated in hemodialysis patients, and rise postdialysis. QT interval and QT dispersion is an easily obtainable, noninvasive, simple, inexpensive, and widely available method of risk stratification in uremic patients receiving chronic dialysis. Additional studies are needed to clarity whether increased postdialysis QT dispersion results in an increased occurrence of arrhythmias.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

가축 분뇨를 이용한 미생물 연료전지의 특성 및 MEA 열화

박권필 ( Kwon Pil Park ),김영숙 ( Young Sook Kim ),추천호 ( Cheun Ho Chu ),정재진 ( Jae Jin Jeong ),안명원 ( Myung Won Ahn ),나일채 ( Il Chai Na ),이정훈 ( Jeong Hoon Lee ) 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.2

고분자전해질 연료전지용 MEA(Membrane and Electrode Assembly)와 가축분뇨를 이용해 미생물연료전지(MFC)를 구동하였다. 여러 균을 혼합해 MFC를 구동했을 때 개별적으로 구동했을 때보다 높은 개회로 전위(OCV)를 나타냈다. 돼지분뇨, 소분뇨, 닭분뇨, 오리 분뇨 중 돼지 분뇨를 이용했을 때 제일 높은 OCV 540mV를 보였다. 그리고 돼지분뇨에서 최고 963mW/m2의 전력이 발생하였다. MFC 구동과정에서 MEA의 Na2+, Ca2+, K+ 이온 및 불순물들에 의한 오염이 MFC의 낮은 성능의 한 원인임을 확인하였다. Microbial fuel cells (MFC) were operated with livestock wastes and PEMFC (Proton Exchange Membrane Fuel Cells) MEA (Membrane and Electrode Assembly). OCV of MFC with mixtures of microbial was higher than that of MFC with single microbial. MFC using pig wastes showed highest OCV (540 mV) among cow waste, chicken waste and duck waste. And the power density of MFC using pig waste was 963 mW/m2. Contamination of MEA withNa2+, Ca2+, K+ ion and impurities was the one cause for low performance of MFC during operation.

S-363 Chemical pleurodesis using mistletoe extracts via spray catheter during medical thoracoscopy

( Sun Hack Lee ),( Jung Seop Eom ),( Tae Hwa Kim ),( Kyu Min Lee ),( Ji Yeon Shin ),( Han Na Lee ),( Bo Hye Song ),( Yong Ki Sim ),( Geewon Lee ),( Jeong Ha Mok ),( Hyo Yeong Ahn ),( Min Ki Lee ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

We present three cases of successful chemical pleurodesis with a liquid solution of mistletoe extract using a spray catheter during medical thoracoscopy. The medical thoracoscopy was performed in all presented cases to remove pleural effusion and conduct chemical pleurodesis to manage symptomatic malignant pleural effusion. A spray catheter was used to instill the mistletoe extract evenly into the pleural cavity, and there were no pleurodesis-related complications. Respiratory symptoms caused by pleural effusion improved after pleurodesis, and successful pleurodesis was maintained for more than 3 months after medical thoracoscopy in all three patients. Lee and Colt reported that instilling lidocaine via a spray catheter is effective for pain control before chemical pleurodesis during medical thoracoscopy. Using a spray catheter in the same way, we performed pleurodesis with a mistletoe extract via a spray catheter in three patients with MPE. A complete response, defined as no pleural effusion within 4 weeks after the last pleurodesis6, was achieved in all three patients. Several studies have used liquid mistletoe extract via a chest tube for chemical pleurodesis. Stumpf et al. reported that the overall response to mistletoe extract pleurodesis was 72% in 20 patients with MPE. Mistletoe extract, which was previously verified as an effective sclerosant for pleurodesis via a chest tube, could be evenly sprayed into the pleural cavity during thoracoscopy using a spray catheter. Distributing the sclerosant evenly into the pleural cavity with a spray catheter may lead to successful pleurodesis, particularly in patients who underwent medical thoracoscopy. In conclusion, We found that instilling the mistletoe extract using a spray catheter was an effective pleurodesis method and could possibly be performed as an alternative to talc poudrage.

Characteristics of Writing in Parkinson’s Disease: Focused on Pen Pressure, Letter Size, and Writing Speed

이한솔(Han Sol Lee),윤진영(Jinyoung Youn),조진환(Jin Whan Cho),안종현(Jong Hyeon Ahn),윤지혜(Ji Hye Yoon),나덕렬(Duk L. Na) 한국언어청각임상학회 2020 Communication Sciences and Disorders Vol.25 No.1

배경 및 목적: 파킨슨병(PD)의 운동 장애는 쓰기 수행에서 글자 크기뿐만 아니라 필압과 속도 측면에도 영향을 미칠 수 있다. 본 연구의 목적은 PD의 쓰기 특성을 필압, 크기, 속도를 중심으로 살펴보고자 하였다. 방법: PD 38명과 정상 성인(NA) 25명, 총 63명을 대상으로, 태블릿 PC와 디지털펜을 가지고 필압, 획의 길이, 쓰기 시간을 측정할 수 있는 소프트웨어를 사용하여 점선 따라 그리기와 문장 쓰기 과제를 실시하고, 필압, 글자 크기, 쓰기 속도를 분석하였다. 결과: 점선 따라 그리기 과제에서 필압은 두 집단 간 차이가 없었고, 쓰기 속도는 PD 집단이 NA 집단 보다 느렸다. 문장 쓰기 과제에서 PD 집단이 NA 집단 보다 필압이 저하되고, 글자 크기가 작으며, 쓰기 속도가 느렸다. 두 집단은 공통적으로 글자 크기와 쓰기 속도 간의 정적 상관이 나타났으며, PD 집단은 필압과 쓰기 속도 간의 정적 상관도 나타났다. 논의 및 결론: 본 연구 결과는 PD로 인하여 강도, 크기, 속도 등의 운동 능력을 조절하는 것의 어려움이 쓰기 수행 시 필압, 글자 크기, 쓰기 속도로 반영될 수 있음을 보여준다. 본 연구는 그간 글자 크기에만 초점이 맞추어졌던 PD의 쓰기 특성에서 필압이나 쓰기 속도와 같이 다양한 요소를 측정하는 것이 중요함을 확인하였다는 점에서 의의가 있다. Objectives: Movement disorders in Parkinson’s disease (PD) can affect not only letter size but also pen pressure and writing speed. The purpose of this study was to investigate the characteristics of writing with focus on pressure, size, and speed in PD. Methods: Sixty-three subjects (38 in a PD group and 25 in a normal adult [NA] group) performed tasks involving drawing along dotted lines and sentence writing using a tablet PC, digital pen, and software that could measure pen pressure, stroke length, and duration. Results: In the task involving drawing along a dotted line, the PD group showed significantly slower writing speed compared with the findings in the NA group. Additionally, in the task involving sentence writing, the PD group showed significantly weaker pen pressure, smaller letter size, and slower writing speed compared with the NA group. Moreover, both groups showed a positive correlation between letter size and writing speed, but only the PD group showed a positive correlation between pen pressure and writing speed. Conclusion: Difficulty in motor control of strength, size, speed, etc. owing to PD is reflected in pen pressure, letter size, and writing speed when performing a writing task. Our results show an importance in measuring multiple factors such as pen pressure and writing speed in the characteristics of writing in PD, which has until now, has been focused only on letter size.

Improved Battery Performance of Nanocrystalline Si Anodes Utilized by Radio Frequency (RF) Sputtered Multifunctional Amorphous Si Coating Layers

Ahn, In-Kyoung,Lee, Young-Joo,Na, Sekwon,Lee, So-Yeon,Nam, Dae-Hyun,Lee, Ji-Hoon,Joo, Young-Chang American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.3

<P>Despite the high theoretical specific capacity of Si, commercial Li-ion batteries (LIBs) based on Si are still not feasible because of unsatisfactory cycling stability. Herein, amorphous Si (a-Si)-coated nano crystalline Si (nc-Si) formed by versatile radio frequency (RF) sputtering systems is proposed as a promising anode material for LIBs. Compared to uncoated nc-Si (retention of 0.6% and Coulombic efficiency (CE) of 79.7%), the a-Si-coated nc-Si (nc-Si@a-Si) anodes show greatly improved cycling retention (C-50th/C-first) of similar to 50% and a first CE of 86.6%. From the ex situ investigation with electrochemical impedance spectroscopy (EIS) and cracked morphology during cycling, the a-Si layer was found to be highly effective at protecting the surface of the nc-Si from the formation of solid-state electrolyte interphases (SEI) and to dissipate the mechanical stress upon de/lithiation due to the high fracture toughness.</P>

2020 Imaging Guidelines for Thyroid Nodules and Differentiated Thyroid Cancer: Korean Society of Thyroid Radiology

Lee Ji Ye,Baek Jung Hwan,Ha Eun Ju,Sung Jin Yong,Shin Jung Hee,김지훈,Lee Min Kyoung,Jung So Lyung,Lee Young Hen,Ahn Hye Shin,Yoon Jung Hyun,Choi Yoon Jung,Park Jeong Seon,Lee Yoo Jin,Choi Miyoung,Na Don 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.5

Imaging plays a key role in the diagnosis and characterization of thyroid diseases, and the information provided by imaging studies is essential for management planning. A referral guideline for imaging studies may help physicians make reasonable decisions and minimize the number of unnecessary examinations. The Korean Society of Thyroid Radiology (KSThR) developed imaging guidelines for thyroid nodules and differentiated thyroid cancer using an adaptation process through a collaboration between the National Evidence-based Healthcare Collaborating Agency and the working group of KSThR, which is composed of radiologists specializing in thyroid imaging. When evidence is either insufficient or equivocal, expert opinion may supplement the available evidence for recommending imaging. Therefore, we suggest rating the appropriateness of imaging for specific clinical situations in this guideline.

Regulation properties of phospholipase Cδ cloned from Misgurnus mizolepis

Na Young Kim,Sang Jung Ahn,Soo Jin Jeon,Jung Soo Seo,Moo Sang Kim,Sang Hwan Lee,Ju Eun Je,Ji Hea Sung,Hyung Ho Lee,June Woo Lee,Joon Ki Chung 한국어병학회 2007 한국어병학회지 Vol.20 No.2