단클론항체를 이용한 타액 내 Streptococcus mutans 수준의 측정

김추성,김재곤,양연미,백병주,이경열,김미아,임수민 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.2

Streptococcus mutans는 구강 내에 상존하는 치아우식증의 주요 원인균으로서 치면의 피막에 부착 후 glucan을 형성하여 세균의 군락을 이루며, 외부로부터 공급된 자당대사를 통하여 유기산을 생성함으로써 법랑질을 탈회시킨다. 치아우식 활성도의 평가를 위한 단클론항체를 이용한 방법은 진료실에서 빠른 시간 내에 간편하게 타액에 존재하는 Streptococcus mutans의 정량분석이 가능한 방법이다. 이 연구는 3세에서 6세 사이의 어린이 15명을 대상으로 자극성 타액을 채취하여 시판 중인 단클론항체를 이용한 Salivacheck Mutans, strip을 이용한 Dentocult-SM 그리고 MSB배지 배양법으로서 타액 내 Streptococcus mutans를 측정한 후 그 값을 우식경험치아수와 비교하여 상관관계를 알아보았다. Saliva-check Mutans를 이용한 방법은 Dentocult-SM과 MSB배지법과 통계학적으로 유의한 상관관계를 보였으나 (p<0.05), MSB배지법은 어린이의 우식경험치아수와 통계학적으로 유의한 결과를 나타내지 않았다 (p=0.34). Streptococcus mutans, one of the major causal agents of dental caries, is component of the dental plaque. It produces various organic acids such as lactic acid which is the end-product of glycolysis, and this leads to dental caries. A new system using species-specific monoclonal antibodies was developed to detect Streptococcus mutans in saliva. The system quickly detects salivary Streptococcus mutans in 30min and classifies the result into two levels. The purpose of this study was to investigate correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods. Children's deft indices were also compared with Streptococcus mutans counts in MSB agar plate. Subjects consisted of 15 children in the age of 3 to 6 years. They were assigned to three groups: Group Ⅰ (deft index = 3), Group Ⅱ (deft index ≤ 3), Group Ⅲ (deft index ≥ 4). The results are as follows : 1. The rate of children with positive response was 13.3% and with negative response was 86.7% in the result of Saliva-check Mutans test kit. 2. There was a positive correlation between monoclonal antibody-based detecting system and selective medium-based detecting methods (p<0.05). 3. Streptococcus mutans counts in MSB agar plate were irrelevant to deft of children(p=0.34).

수면량과 집중력의 상관관계

김병우,류지근,박우진,박계원,김미정,임지은,백대현,최재덕,최윤호,이가현,이관,김덕수 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1

수면, 기억 그리고 학습의 연관성은 실제 매우 복잡하다. 많은 연구에도 불구하고 명확한 연구결과는 아직 없다. 우리는 이 연구에서 평소와는 달리 시험 전날 수면 변화 양상이 집중력에 어느 정도 영향력을 미치는지 알아보고자 하였고, 이를 이용해 효율적인 수면 관리를 할 수 있는 기초자료로 삼고자 하였다. 동국대 의대 본과 1학년생 중 지원자 30명과 본과 2학년생 10명을 대상으로 평상시 상태와 시험 직후 수면이 부족한 상태에서 각각 K-PES TEST를 실시하여 총 8항목에 걸쳐 암기력, 판단력, 단순반응의 변화정도를 검사하였다. 또 설문조사를 병행하여 결과에 영향을 미칠 수 있는 다른 인자들도 파악하였다. 수면량에 대한 부호 숫자 짝짓기(순간 판단력)을 검사한 결과, 인간의 최대 생리학적 기능은 꼭 수면부족에 의해 좌우되는 것은 아니다. 그러나, 수면감소는 피로를 증가시키고, 동기부여를 감소시키나 단, 새로운 사실을 학습하는 데에는 큰 영향을 끼치지 않는다. 즉, 수면부족으로 장시간 깨어있는 것은 정확성과 반응성을 떨어뜨린다. 흡연에 대한 부호 숫자 짝짓기(순간 판단력)을 검사한 결과, 니코틴의 수면에 대한 상호관계는 알려지지 않았고, 인간의 감정이나 의식, 인식에 영향을 주는 것으로 알고있지만, 장기적은 측면에서 흡연이나 알코올은 인식능력과 무관한 것 같다. 카페인에 대한 숫자 외우기(암기력)검사 결과, 카페인은 spacial selective attention에는 특별한 영향을 주지 않지만 지각과정이나 주의 집중에 큰 효과를 주며, 인간의 의식 수행에 긍정적인 효과를 준다. 즉 카페인은 활력, 혼란, 피로상태를 변화시키고 visual analog scales에서 휴식상태에 가까운 양상을 나타낸다. 또 48시간 동안 수면을 취하지 않은 사람에게 카페인은 집중력과 장시간의 좋은 기분을 유지하게 하였다. 그러나 규칙적, 비규칙적으로 먹는 사람간에는 인지수행력의 차이를 보이지 않으며 상용자에서는 상당한 내성이 보인다. 수면패턴에 대한 숫자 더하기(순간 판단력)검사 결과, 집중력은 수면패턴에 의해서도 영향을 받고, 수면패턴이 바뀌게 되면 순간판단력을 저하시킨다. This study was performed to evaluate relationship of sleep and attention, and to control sleep effectively. We conducted a K-PES test of 40 medical students. K-PES contains eight elements, for example, simple response, selective response, color pairing, OddEven discrimination, number addition, number-symbol matching, memory, right-tapping, left tapping. Through these, we tested changes of memory, judgement, simple response. And we conducted a questionnaire survey to exclude bias. Results are as follows. As a results of number-symbol matching test, we know that sleeplessness is not complete responsible for physiologic function for humans. But, decreased sleep increases fatigue and decreases motivation, but no influence on learning new materials. So, long term sleeplessness decreases precision and responsibility. As a results of number-symbol matching test on smoking, a relationship of nicotine and sleep is not certain. A point of view in long term period, smoking and drinking is not related cognition, even if we know that those influence mood, consciousness and cognition. As a results of memory work test on caffeine, caffeine does not influence spacial selective attention, but mainly does perception, attention and consiousness process. Namely caffeine changes energetic activities, chaos, fatigue states and shows resting state in visual analog scales In sleeplessness for 48 hours, caffeine produced attention and good mood for a long time. But, in case of cognitive process no significance shows in regular or irregular diet. Regular diet shows high resistance. As a results of number addition test on sleep patterns, seep patterns influence attention, changes of sleep patterns decrease a moment judgement.

장간막 낭종 : 1예 보고

이미숙,김윤신,이미자,김용임,임성철,전호종 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

Mesenteric cyst is a rare intraabodominal mass which is composed of most fibrous tissue with focal lining by flattened cells, cholestrol clefts and foam cells but lack of smooth musscle components and elements of the Iymphatic system. And, this entity is frequently misdiagnosed preoperatively as a cystic Iymphangioma. Therefore, histologic and ultrastructural evidence suggest that they are pathologically distinct. It must be distinguished from other intraabdominal and retroperitoneal cyst because outcome of surgical treatment is different. We report a case of mesenteric cyst arising in mesentery of ascending colon in a 46 year old male who had developed as a presenting symptom of periumbilical pain for 2 days before admission, with review of literatures.

數 種 韓藥의 아세틸콜린에스터레이즈 酵素活性에 대한 효과

최재환,김미영,김현미,최훈,전훈,김대근,임종필,임강현 대한본초학회 2002 大韓本草學會誌 Vol.17 No.1

아세틸콜린에스터레이즈(AChE)는 신경조직에 분포하는 효소로 신경전달물질 중 아세틸콜린(ACh)을 분해하는 효소로 시냅스 신호전달체계에 있어 중요한 역할을 하는 효소이다. 현재 알츠하이머병과 치매치료에 사용하는 서양약물은 AChE의 활성을 저해하는 작용기전을 가진다. 목적 : 전통적으로 기억력 감퇴, 치매 등 치료에 사용되어 온 한약의 AChE에 대한 작용을 밝히기 위해 본 실험이 구상되었다. 방법 : 생쥐 대뇌신경세포의 균질 혼합액으로부터 효소의 활성을 Ellman 방법을 개선한 엘리자(ELISA)법을 이용하여 측정하였다. 결과 : 실험결과 天花? 의 6종이 2.5㎎/㎖의 농도에서 우수한 효소활성저해력을 보였고, 50% 효소활성 저해농도(ID_50)를 구한 결과, 天花?의 ID_50은 1.5㎎/㎖이었고, ??? 1.2 ㎎/㎖, ?? 2.0 ㎎/㎖, ??草 1.2 ㎎/㎖, ??? 1.0 ㎎/㎖, ??生 1.7㎎/㎖, 및 大?皮 2.0 ㎎/㎖를 나타내어 忍冬?의 저해력이 가장 우수하였다. 결론 : 치매 및 기억력감퇴 증상을 치료하는 한약의 작용기전 중 AChE 효소활성억제효과는 忍冬? 의 6종에서 나타났으며, 이는 전통적인 한방치료에 과학적인 기초자료를 제공할 것으로 생각한다.

산성강하물의 침착량과 동태 해명에 관한 연구 -춘천과 서울 강우의 화학조성 비교 : Comparison of Chemical Composition of Rain Water between Chunchon and Seoul

김만구,강미희,임양석,박기준,황훈,이보경,홍승희,이동수 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

The rain water samples were collected at Chunchon and Seoul by using wet only automatic sampler from January 1996 through December 1997. The daily base rain water samples collected over than 95% rainy events during the whole period. The samples were measured both pH and conductivity, and were analysed major ionic components, SO₄^(2-), NO₃^(-), Cl^(-), NH₄^(+), Ca^(2+), Na^(+) and K ^(+), by ion chromatography. In 1996, about 77% sampled rain water showed below pH 5.6 and the 60% of rain water was lower than pH 5.0. The volume weighted average pH was 4.7 at all sites. In 1997, the volume weighted average pH was 4.6 and 4.9 at Seoul and Chunchon, respectively. Among the rain water samples, 87% and 55% of samples showed below than pH 5.6 and 5.0, respectively. The pH value of Chunchon was significantly (p<0.05) lower than Seoul at the rain samples for less than 20 ㎜ rainfall. However conductivity of the rain samples were 20.9 μS/㎝ for 1996 and 27.7 μS/㎝ for 1997 at Seoul, and 19.1 μS/㎝ for 1996 and 14.1 μS/㎝ for 1997 at Chunchon. H₂SO₄AND HNO₃CONTRIBUTED 65.9% and 29.6% of free acidity at Seoul, respectively. The ratio of [NO₃^(-)]/[nss-SO₄^(2-)] were 0.43 at Seoul and 0.51 at Chunchon for rain samples for less than 20 ㎜ rainfall. The annual wet deposition of Cl^(-), NO₃^(-), SO₄^(2-), H^(+), Na^(+), NH₄^(+), K ^(+), Mg^(2+) and Ca^(2+), respectively, 568.8 ㎏/㎢, 1489.3 ㎏/㎢, 3184.8 ㎏/㎢, 20.9 ㎏/㎢, 249.4 ㎏/㎢, 1091.2 ㎏/㎢, 189.8 ㎏/㎢, 90.2 ㎏/㎢, and 702.4 ㎏/㎢ at Seoul for 1996; 656.4 ㎏/㎢, 2029.7 ㎏/㎢, 3280.7 ㎏/㎢,27.2 ㎏/㎢, 229.4 ㎏/㎢, 1063.9 ㎏/㎢, 106.9 ㎏/㎢, 7802 ㎏/㎢, and 645.3 ㎏/㎢, at Seoul for 1997; 116.9 ㎏/㎢, 983.3 ㎏/㎢, 1797.0 ㎏/㎢, 21.4 ㎏/㎢, 83.2 ㎏/㎢, 648.1 ㎏/㎢, 78.0 ㎏/㎢, 22.2 ㎏/㎢ and 368.8 ㎏/㎢ at Chunchon for 1996; 100.2 ㎏/㎢, 1077.6 ㎏/㎢, 1754.0 ㎏/㎢, 13.4 ㎏/㎢, 146.0 ㎏/㎢, 602.3 ㎏/㎢, 88.8 ㎏/㎢, 16.2 ㎏/㎢ and 206.8 ㎏/㎢ at Chunchon for 1997.

만성 요통환자의 복부 심부근과 표재근을 중심으로 한 운동 효과 비교

김진산,이창현,조미주,임연욱,정취산,김선엽 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.1

Objectives: To compare the efficacy of two types specific exercise intervention (deep and superficial abdominal muscle exercises) in the treatment of patients with chronic low back pain (CLBP). Design: A randomized, comparative, repeated-measures. Subjects: Twenty-four patients with CLBP (at least for 3 months), mean age±SD=51.58±16.21 (yrs). Methods: Twenty-four patients with this condition were assigned randomly to two treatment groups. Both first and second group underwent 3 weeks specific exercise treatment program. The first group specific trained for the deep abdominal muscles. The second group specific trained for the superficial abdominal muscles. Results: After intervention, the first group showed a statistically significant reduction in pain intensity and functional disability levels (p<.05). The second group showed no significant change in these parameters after intervention (p>.05). Conclusions: According to results, a "deep abdominal muscle exercises" treatment approach appears more effective than a "superficial abdominal muscle exercises" treatment in patients with CLBP.

유방에서 기원된 전이성 췌장암종 1예 : A case report

김윤신,이미숙,장원재,임성철,기근홍,전호종,서재홍 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

Pancreas is known for the unfavorable soil to the metastatic carcinoma, and pancreatic metastasis is achieved after systemic dissemination was developed. The primary tumors of metastatic pancreatic carcinoma are breast, lung, Iymphoma, malignant melanoma, leukemia, stomach. and colon. The breast carcinoma is the commonest tumor to metastasize to pancreas and both tumors are related to estrogen receptor for tumor growth. In the present case, tumor cells had small round nucleus and showed indian file infltrating pattern and lack of cohesion, which are seen in infiltrative breast carcinoma. The patient was underwent radical mastectomy due to infiltrative ductal carcinoma three years ago. And also the tumor cells showed nuclear reactivity to immunostaining for estrogen receptor In combination of the histopathologic. immunohistochemical, and her past history, we concluded that breast carcinoma was metastasized to pancreas, and we report a case of metastatic pancreatic carcinoma from breast with review of literatures.

식용대두유에 대한 뽕잎추출물의 항산화 작용

김미원,안명수,임영희 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.1

Recently, the concern about safety and functional substances of foods are increased therefore antioxidant substances and plants which had pharmacological effect have been studied actively. It has been known that the mulberry leaf is effective in curing many diseases. Nowaday, the pharmachological effects of mulberry leaves on diabetes mellitus and their usage for many aspects were confirmed. Mulberry leaves are known for effective in prevention of diabetes mellitus, because of specific amino acids and fibers. In this study, methanol, hexane, chlorform, ethyl acetate, and butanol extracts obtained from mulberry leaves were added to soybean oil and they were stored for 30 days at 60±2℃ and peroxide value(POV) and conjugated diene value(CDV) were measured periodically. Results of this study were obtained as follows; 1. The POV of soybean oil after the addition of each mulberry leaves powder(MLP) extracts generally enhanced as the storage time was prolonged, so the POV of all samples was reached higher than 100meq./kg.oil after 10 days storage without the addition of butanol, methanol, ethylacetate, hexane extracts at 0.1% level. Especially, the POV of soybean oils including butanol extract was 87.35meq/kg.oil after 10 days storage and antioxidant activity of butanol extract was shown to be superior to that of BHT. The pattern of the changes of the CDV of soybean oil after the addition of MLP extracts at 0.02%, 0.05% and 0.1%, respectively, were almost constant during 10 days of storage and then rapidly increased during the rest of experimental periods. During 10 days of storage in case of 0.1% adding level, the antioxidant activities of the butanol extract was superior to that of the each MLP extracts.

각종 전분의 이화학적 특성 및 가공식품의 이용에 관한 고찰 : 국내 학회지를 중심으로

임영희,김미원 대전대학교 생활과학연구소 2000 생활과학연구 Vol.6 No.-

Literatures about starches have been published on the journal of association from 1970th to 2000. Study of acorn starch studied steadly from 1970th and trend of these studies was mainly about physio-chemical nature, physical characteristics, molecular structure and functional characteristics. Studies of corn starch are total 12 articles and was started from 1980th. Cowpea starch and mung bean starch were studied comparatively and studied mostly about physio-chemical characteristics and reology. Studies of chestnut starch started since 1990th and chestnut starch is expected to large amount of consumption due to possibility of being made to chestnut jelly. In addition, there are 8 articles about bean starch from 1980th, and studies about rice starch were studied simultaneously. Reserches for potato and sweet potato starch were also carried out, and potato starch was studied about gelatinization and starch jelly, and studies of sweet potato was mostly about physio-chemical characteristics. And Studies of sweet potato were started at 2000. The other studies of starch were about barley starches, yam starch, buckwheat starch, starch, and arrowroot starch.

대전대학교 기숙사 식당의 급식만족도 조사

임영희,김미원,곽현옥,박상현 대전대학교 생활과학연구소 2002 생활과학연구 Vol.8 No.-

This study aimed at examining the foodservice satisfaction on dormitory restaurant by gender, age, college. The subjects of the study were 236 students living in the dormitory of Daejeon University. The survey was conducted between June 4 to June 12, 2002. The data was collected by survey and- was analyzed by SPSS package. The evaluation of the foodservice satisfaction by gender, showed that answers of the most students were below average. Generally, male students were more satisfied than females. The evaluation of the foodservice satisfaction by age, showed that younger students were more disatisfied than older students. The results suggest that food preference of subjects should be considered in meal planning and various cooking method should be utilized to enhance taste of foods for better foodservice. The management of the foodservice will be made efforts to improve the quality of foodservice on the basis of these results.