Neuroprotective effects of bovine colostrum on intracerebral hemorrhage-induced apoptotic neuronal cell death in rats

Kim, Sung Eun,Ko, Il Gyu,Shin, Mal Soon,Kim, Chang Ju,Ko, Young Gwan,Cho, Hanjin Medknow PublicationsMedia Pvt Ltd 2012 Neural regeneration research Vol.7 No.22

<P>Brain cell death after intracerebral hemorrhage may be mediated in part by an apoptotic mechanism. Colostrum is the first milk produced by mammals for their young. It plays an important role in protection and development by providing various antibodies, growth factors and nutrients, and has been used for various diseases in many countries. In the present study, we investigated the anti-apoptotic effects of bovine colostrum using organotypic hippocampal slice cultures and an intracerebral hemorrhage animal model. We performed densitometric measurements of propidium iodide uptake, a step-down avoidance task, Nissl staining, and caspase-3 immunohistochemistry. The present results revealed that colostrum treatment significantly suppressed N-methyl-D-aspartic acid-induced neuronal cell death in the rat hippocampus. Moreover, colostrum treatment improved short-term memory by suppressing hemorrhage-induced apoptotic neuronal cell death and decreasing the volume of the lesion induced by intracerebral hemorrhage in the rat hippocampus. These results suggest that colostrum may have a beneficial role in recovering brain function following hemorrhagic stroke by suppressing apoptotic cell death.</P>

미치광이풀 모상근 배양에서 Tropane Alkaloid 생산성 증진을 위한 최적 생물학적 엘리시터 선발

정희영,강승미,강영민,김용덕,양재경,정영관,최명석 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Scopolamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supematant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

제4형 가족성 고중성지방혈증 임산부에서 발생한 급성 췌장염 1례

박관응,윤채중,김영학,진영기,윤성호,권용은,김태원,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.2

Plasma levels of cholesterol (TC) and triglyceride(TG) physiologically increase during pregnancy. The lipid increment is 23-53% above the pregravidic level for TC and 2- to 3-fold of the prepregnancy level for TG. If the TC and TG are higher than normal values in pregnancy, the patient must be carefully monitored. Acute pancreatitis is the main consequence of hyperlipidemia and occurs either during pregnancy, in the third trimester, or in the puerperium. Mortality is high both for the mother (21%) and the fetus (20%). We report a case of 28 year-old pregnant woman at 29 weeks gestation with hypercholesterolemia (TC = 357 ㎎/dl) and severe hypertriglyceridemia (TG = 1300 ㎎/dl). The patient was admitted to the hospital because of severe epigastric pain, nausea and vomiting. Total serum cholesterol was increased and trigyleride was markedly increased. The electroporesis pattern of serum lipoprotein showed increase in pre-beta lipoprotein fraction, suggesting IV hypertriglyceridemia pattern. According to a review of the literature, the incidence of pancreatitis during pregnancy is 1 in 1000 to 3000 pregnancies. Severe hypertriglyceridemia in pregnancy should be treated with a careful restriction of calories and fat: for preventing acute pancreatitis, hospitalization for intravenous fluid therapy and plasma exchange must be required.

일측성 질폐쇄 및 동측 신장 무발생을 동반한 중복자궁 2예

윤일영,여소진,김형문,김정식,김태희,이해혁,남계현,이권해,김준모,김민의 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Uterus didelphys with unilateral obstructed hemivagina and ipsilateral renal agenesis usually presents after menarch with progressive abdominal pain during menses secondary to hematocolpos, and indeed a very rare congenital anomaly due to Mullerian duct malformation. An accurate and prompt diagnosis is of importance to permit treatment and to assure the future fertility of the patient. Pelvic ultrasound, physical examination and pelvic MRI established a diagnosis of hematometracolpos secondary to uterus didelphys with unilateral imperforate hemivagina. An incision in the vaginal septum allowed drainage of the hematocolpos, providing relief of the patients symptoms. We report two case of uterus didelphys with obstructed hemivagina with brief review of the literature.

혈소판 풍부혈장의 임상적 응용

하정완,김수관,조세인,이철우,정태영,김수흥,김영균 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.5

Platelet-rich plasma is an autologous source of platelet-derived growth factor and transforming growth factor beta that is obtained by sequestering and concentrating platelets by gradient density centrifugation. We have used platelet-rich plasma for bone graft, especially allobone graft, at implant surgery, sinus lift procedure, and cyst enucleation. This article is retrospective study from October 1999 to November 2000. All cases were good healing and clinical success.

H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

貯藏中인 쌀(벼, 玄米, 自米 Parboil米)의 脂肪成分의 化學的 變化

金關修,盧永姬,李熙鳳 충북대학교 농업과학기술연구소 1994 農業科學硏究 Vol.11 No.2

Rough rice(Chucheong) which had been harvested at the experiment station, Chung Buk National University on October 26, 1992, was used in this experiment. The rough rice was divided into two parts. A part of the rough rice was parbolied(B series) and another part was untreated(A series). Brown and polished rice was obtained from untreated and parboiled rice. Six different types of the rice stored at room temperature for about 10 months. During the storge time, moisture and crude lipid contents, free fatty acid values, peroxide values, TBA values and fatty acid compositions were investigated. The result was as follows. 1. The moisture contents of all the samples decreased remarkably during the storage periods. Polished rice showed the greatest decrease. The decreasing rate was pronounced between 3-7 mouth. 2. The crude lipid contents of the polished and brown rice increased during the storage periods. As the moisture contents decrease, the crude lipid contents was increased proportionally. The crude lipid contents of rough rice were rarely changed. 3. The free fatty acid values of all the samples increased during the storage periods. The increasing rate was pronounced between 5-9month. The free fatty acid values of polished rice was greater than those of brown and rough rice. 4. Peroxide values of all the samples increased during the storage periods. Especially, the increase in the peroxide values remarked between 5-9month. The peroxide values of untreated rice were greater than those of parboiled rice. 5. TBA values of all the samples increased during the storage periods. Its increase pattern were similar to the peroxide values. 6. Major fatty acids of the samples were oleic acid, and linoleic acrid, palmitic acid. A little of stearic acid, myristic acid, linolenic acid, and arachidic acid was found. The changes in fatty acid composition of the each samples were rarely noted during the storage periods.

Pilot plant와 Jar test를 이용한 응집제 LAS와 PAC의 수처리 효과 비교

김운중,고창옥,조영관 朝鮮大學校 建設技術硏究所 2001 建設技術硏究 Vol.21 No.1

Juam raw water was used to investigated water treatment efficiency in terms of coagulants and experiment types as jar-test and lab-scale pilot plant. pH of treated water was about 0.1~0.2 high when pilot plant was used than when jar-test was used in all of coagulants as liquid aluminium sulfate(LAS) and poly aluminium chloride(PAC). In comparing pH of surface water between in actual precipitation process with in pilot plant precipitation process when LAS was used, variation range of pH was 0.16 in pilot plant process whereas 0.04 in actual process. In comparing color, turbidity, zeta-potential, aluminium of precipitated surface water, it showed same results in all of coagulants as PAC and LAS and all of processes. In comparing alkalinity of the water, PAC showed alkalinity was 2∼3ppm higher than when LAS was used because of coagulants' characteristics as Al2O3 contents. Langarier indices as Corrosive index were -2.95 in LAS and -2.66 in PAG, which means PAC has less corrosive than LAS. In, comparing the removal of algae using LAS and PAC in jar-test and pilot plant, the removal rates were 80% in jar-test and 75% in pilot plant. The rate was improved to 90% after when incline baffles were used. Therefore, PAC is appropriate in considering prevention of corrosion, on the other side, LAS is appropriate in considering economy.

레이저 용융 적층공정을 이용한 정밀 형상 제작

김재도,전병철,권택열,이영곤,신동훈 한국레이저가공학회 2000 한국레이저가공학회지 Vol.3 No.3

For the direct metal shape processing the powder feed device which is different from the widely used in rapid prototyping. Is developed, The three dimensional object is shaped with the melting metal powder. The developed research has applied to rapid prototyping in ultraprecision for MEMS and medical science fields required of rapid manufacture of complex shape. The goal of this study make 3D model which has precision accuracy. Powder spreading apparatus has been more improved because that the control of powder spread is very important in layer manufacturing. It consists of the vibration motor, nozzle and tube which supplies various metal powder. This apparatus could control the spreading velocity that could control powder spreading thickness. Laser on/off switch was adapted because laser scanning velocity must be preserved constantly to prevent heat transformation of laser overheating. The error between sintered thickness and experimental one occurred by shrinkage in sintering melting process. The problem of heat transformation was solved by On/Off switching system.