Inhibitory Effect of Farfarae Flos Water Extract on COX-2, iNOS Expression and Nitric Oxide Production in lipopolysaccharide - activated RAW 264.7 cells

Yoon Tae Gyoung,Byun Boo Hyeong,Kwon Teag Kyu,Suh Seong Il,Byun Sung Hui,Kwon Young Kyu,Kim Sang Chan The Physiological Society of Korean Medicine and T 2004 동의생리병리학회지 Vol.18 No.3

Farfrae Flos has been clinically used for the treatment of asthma in traditional oriental medicine. There is lack of studies regarding the effects of Farfrae Flos on the immunological activities. The present study was conducted to evaluate the effect of Farfrae Flos on the regulatory mechanism of cytokines and nitric oxide (NO) for the immunological activities in Raw 264.7 cells. In Raw 264.7 cells stimulated with lipopolysaccharide (LPS) to mimic inflammation, Farfrae Flos water extract inhibited nitric oxide production in a dose-dependent manner and abrogated inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2). Farfrae Flos water extract did not affect on cell viability. To investigate the mechanism by which Farfrae Flos water extract inhibits iNOS and COX-2 gene expression, we examined the on the phospholylation of inhibitor κBα and production of TNF-α, IL-1β and IL-6. Results provided evidence that Farfrae Flos inhibited the production of interleukin-1β (IL-1β) and the activation of phospholylation of inhibitor κBα in Raw 264.7 cells activated with LPS. These findings suggest that Farfrae Flos can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections.

桑黃 물추출물이 LPS로 유도된 Raw 264.7 cell에서의 TNF-α, IL-1β, IL-6 및 Nitric Oxide production에 미치는 영향

김상찬(Sang Chan Kim),정연숙(Youn Suk Jung),이재령(Jae Ryung Lee),김영우(Young Woo Kim),변부형(Boo Hyeong Byun),권택규(Teag Kyu Kwon),서성일(Seong Il Suh),변성희(Sung Hui Byun),권영규(Young Kyu Kwon) 한의병리학회 2004 동의생리병리학회지 Vol.18 No.3

Interleukin-2와 결핵균 30 kDa 항원이 구개편도 및 말초혈액 T 세포 증식에 미치는 상승효과

박정규,박찬권,조은경,김화중,백태현,고필준,김병국,남부현,나기상,박찬일 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

Widespread use of BCG has not controlled tuberculosis, and more effective vaccines are clearly needed. Although chemotherapy will remain the mainstay of antituberculosis treatment, the use of adjunctive immunotherapeutic modalitites is attractive, particularly in persons with drug-resistant tuberculosis. Administration of IL-2 or IFN-γto tuberculosis patients enhance bacillary elimination. Cell-mediated immunity is the critical protective immune response in tuberculosis. Mycobacterial antigens are recognized by T cells and that elicit production of protective cytokines are potentially important vaccine antigens. The 30 kDa antigen is secreted in large quantities by growing mycobacteria. That antigen elicits greater proliferation in lymphocytes from healthy tuberculin reactors than healthy tuberculin nonreactors. In this study, the T lymphocyte proliferative responses to 30 kDa antigen from Mycobactrium tuberculosis H37Rv were examined by using tonsilar and peripheral blood lymphocytes from PPD(+) and PPD(-) tonsilectomized persons. When cultured with 30 kD antigen, tonsilar mononuclear leukocytes and T cells of PPD(+) demonstrated more ^3H-thymidine incorporation than PPD(-) persons (stimulation index was 2.5 and 1.9, 0.8 and 1.0, repectively). Peripheral blood mononuclear cells (PBMC) and peripheral blood T lymphocytes were shown the similar responses to this antigen. The combination of IL-2 and 30 kDa antigen elicited a significant proliferative responsiveness in tonsilar mononuclear leukocytes and T cells of PPD(+) persons (SI was 20 and 14.1). PBMC and peripheral blood T cells of PPD(+) persons were also shown a significant responsiveness, but PPD(-) persons did not show. These results demonstrate that the 30 kDa antigen and IL-2 have a synergistic stimulatory property in mycobacteria sensitizing lymphocytes.

척수손상·비손상인의 신체부위별 신체구성 및 골밀도 비교

김성수,황부근,김태수,이충일,양정수,이동희 한국특수체육학회 2003 한국특수체육학회지 Vol.11 No.1

This study was designed to compare segmental body composition and bone mineral density in spinal cord-injured(SCIS; n=9, SCIA; n=9) and non-injured control(AB; n=9) subjects. Dual-energy X-ray absorptiometry(DEXA) was used to measure %body fat, body fat mass, lean body mass, bone mineral contents, and bone mineral density in total and various body regions(arms, legs, trunk). The present results showed that body fat mass and %body fat were significantly higher and lean body mass was significantly lower in the SCIS group compared to AB group, while there was no difference between the SCIA and AB group in those of parameters. In BMC, there was no difference among groups and in BMD, SCI group was significantly lower that AB group, but there was no difference between SCIA group and AB group. Based on these results, in can be suggested that long-term regular exercise can reduce the negative changes in body composition, bone mineral density by spinal cord injury.

운동 강도의 차이가 흰쥐의 혈액 성분과 적혈구의 형태에 미치는 영향

김부환,김종오,지용석,최대원,윤진환,정일규,김영욱,오봉석 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

This study was to investigate the effects on hematological indices and heinz body formation in various intensity, Male Sprague-Dawley rats were randomly divided into the control group, the low-intensity exercise group, nigh-intensity exercise group (n=7 in each group). Animals of the low-intensity exercise groups were put on exercise 30 min per 1 day, 5 days per week for 6-weeks. Animals of the high-intensity exercise groups were put on exercise 120 min per 1 day, 5 days per week for 6-weeks. Red blood cell count (RBC), hemoglobin (Hgb), hematocrit (Hct), mean ceil volume (MCV), mean hemoglobin corpuscular (MCH), mean hemoglobin corpuscular concentration (MCHC) were analyzed in automated hematology analyzer (ABX Hematology MICROS 60) from 10㎕ of whole blood. Erythrocyte morphology were evaluated in super vital (Brilliant cresyl blue stain) stained blood films. In the present results, Hg, MCHC in the high-intensity exercise group was significantly decreased compared with the different exercise intensity and the control group. MCV in the high-intensity exercise group was significantly increased compared with the different exercise intensity end the control group. Heinz body formation in the high-intensity exercise group was significantly increased compared with the different exercise intensity and the control group. In the present study, it can be suggested that intensity of exercise can influence hematological variables and heinz body formation. These findings suggest that high intensity exercise induces oxidative damage to erythrocyte.

후외상성 진주종 2례

남부현,김병국,고필준,심장한,박찬일 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.1

Post-traumatic cholesteatoma can be originated in the middle ear or mastoid cavity after head trauma such as temporal bone fracture, penetrating injury of the temporal bone or blast injury of the tympanic membrane. It usually takes a long time to develope cholesteatoma after injury, and physicians may overlook its association with previous, trauma. Post-traumatic cholesteatoma can be suspected in patients with head trauma history and atypical cholesteatoma findings. We experienced two cases of cholesteatoma which found behind the intact tympanic membrane in 7 and 13 years old boys with history of head trauma, and we are reporting this with a discussion and literatural review.

韓國産 紅花子와 中國産 紅花子가 鎭痛 , 消炎 및 關節炎에 미치는 效果

서부일,이은숙,박지하,김상찬,변부형,최호영 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

This experimental study was done to compare the efficacy of Korean and Chinese Carthami Semen on analgesic effect and remedy of inflammation and arthritis. The results were as follows; 1. In analgesic action on the writhing syndrome in mice induced by 0.7% acetic acid, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in analgesic action on the writhing syndrome in mice induced by Acetylcholine HC1, Korean and chinese Carthami Semen extract group showed significant decrease in comparison with control group. 2. In Anti-inflammatory effects, Chinese Carthami Semen extract group showed significant decrease at 150 and 180 minutes after inducing paw-edema in comparison with control group. 3. In the method of adjuvant arthritis, orally administered for 21 days, Korean Carthami Semen extract group showed significant inhibition of the hind paw edema after 1 day and 21 days. Whereas Chinese Carthami Semen extract group showed some reduction, but the results didn's show significant decrease. 4. In the level of leucocytes count, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in the level of total protein. CRP and glucose, Korean and Chinese Carthami Semen extract group didn't showed significant change in comparison with control group.

폴리프로필렌 섬유보강 콘크리트(PFRC)의 역학적 특성 및 내구성에 관한 실험적 연구

박승범,김부일,윤준석 忠南大學校 産業技術硏究所 1998 산업기술연구논문집 Vol.13 No.1

The result of an experimental study on the mechanical properties and durability of polypropylene fiber reinforced concrete are presented in this paper. This study has been performed to obtain the properties of PFRC such as strength toughness and durability. The test variables are fiber content fiber types, W/C ratio. PFRC shows the highest strength when the polypropylene fiber content is about 0.2% in concrete, flexural toughness of PFRC was increased remarkably as polypropylene fiber contents were increased to 2.0 vol.%. Also, freeze-thaw resistance and carbonation were somewhat more improved than plain concrete.

생분해성 플라스틱이 포함된 유기성 생활 쓰레기의 퇴비화

신창호,김영진,이상일,장상희,조무환,신부영 영남대학교 환경문제연구소 2000 環境硏究 Vol.19 No.2

준 생산용 퇴비화 장치를 이용하여 생분해성 플라스틱을 포함하는 유기성 도시생활쓰레기기의 퇴비화를 연구하였다. 유기성 도시생활쓰레기는 실제 도시생활쓰레기 구성비에서 무기물을 제외한 유기성 물질만의 비로 만들어 사용하였다. 유기성 도시생활쓰레기를 구성하는 각각의 물질에 대한 생분해도를 ISO법으로 측정하였고, 퇴비의 화학적 물리적 특성도 조사하였다. 구성 물질에 대한 생분해도 결과에서, 생분해성 고분자를 포함한 각 물질들은 퇴비화에 충분한 생분해도를 나타내는 것으로 밝혀졌다. 또한, 유기성 도시 생활쓰레기로부터 비독성의 퇴비를 얻을 수 있음을 알았다. The composting study of organic municipal solid waste containing biodegradable plastic was performed by using pilot scale composting apparatus. The organic municipal solid waste(OMSW) was artificially prepared for composting. The composition of artificial OMSW was the same as that of real municipal solid waste excluding inorganic waste. The biodegradability of individual component of OMSW was studied according to the ISO method. The chemical and physical characteristics were also observed. Biodegradability study revealed that every component of OMSW including biodegradable plastic had sufficient degradability for real composting. And it was found that non-toxic compost could be produced from OMSW containing biodegradable plastic.

필드버스시스템의 효율적인 테이터 서비스를 위한 온라인 토큰제어

이석원,남부희,김일환 강원대학교 정보통신연구소 1998 정보통신논문지 Vol.2 No.-

A fieldbus system should be designed by considering the number of sensors/actors(actuators+controllers)and their sampling times. But the munber of sensors/actors which are connected to the network bus can be varied by addition and/or due to the fault of the field devices. That variation decreases the efficiency of the system which is off-line designed optimally. This paper proposes an algorithm that ensures the transmission of cyclic data of the control system where there can be the number change in the field devices. We assign the different weight values to cyclic data and acyclic data. By simulation and experiment, the cyclic and acyclic data are served for the proposed network and the Profibus system. It is shown that the proposed algorithm is more robust with the uncertainties of the field devices of the control system.