Growth inhibition of human hepatoma and bladder carcinoma cells by DNA topoisomerae inhibitor β-lapachone

Hyun Joo Woo,Yung Hyun Choi 한의병리학회 2006 대한동의병리학회 학술대회논문집 Vol.2006 No.9

단일 한의원 교통사고 환자 258례에 대한 한방치료 효과 후향적 연구

황지혜(Ji Hye Hwang),정철(Chul Jung) 한의병리학회 2019 동의생리병리학회지 Vol.33 No.3

In South Korea, traffic accident patients can be treated under automobile insurance coverage. This study investigated the status of Korean medicine (KM) Automobile insurance system and usage status of main pharmacopuncture in KM clinic which reported that the largest number of automobile insurance fee claims among medical institutions. We surveyed 258 traffic accident patients who were treated at Namsangcheon KM clinic from 2014 to 2018 according to medical chart. The majority of the patients were male and thirties. In traffic accident situation, the highest distribution was car to car crash with 85.66% and 66.67% of the patients visited in the most acute phase. The most frequent treatment period was within 4 weeks and the number of treatments was 10 or less with 72.87%. In total results of treatment, the distribution was exellent with 10.08%, good with 46.90%, fair with 27.13% and poor with 15.89%, and the effective rate was 84.11%. The most frequent treatment period was within 4 weeks with 64.73% and the number of treatments was 10 or less with 72.87%. Of the 242 patients who received pharmacopunture, 91.5% were treated with HO, which was named after Honghwaja and TA, which was named after traffic accidents, and there were significant differences in the number of treatments and symptom improvement between the two groups. In this study, we confirmed the status of automobile insurance treatment and usage of main pharmacopuncture of single KM clinic with symptom improvement. This study can be regarded as one of the basis of KM treatment for the rapidly growing automobile insurance market.

사람 피부 섬유아세포에서 자외선으로 유도된 기질분해효소-1과 기질분해효소-3의 발현 유도에 대한 사상자 추출물의 억제효과

노은미(Eun Mi Noh),송현경(Hyun Kyung Song),김정미(Jeong Mi Kim),이금산(Guem San Lee),권강범(Kang Beom Kwon),이영래(Young Rae Lee) 한의병리학회 2019 동의생리병리학회지 Vol.33 No.3

Torilis Japonica (TJ) has been used as an anti-allergy, antifungal, and antibacterial agent. Recent studies have reported that it also shows anti-cancer effects. It is report that TJ inhibits melanin synthesis in melanocyte in the skin. However, the effect and mechanism of TJ extract (TJE) on Ultraviolet (UV)B-induced photoaging are unknown. In this study, we investigated the preventive effects of TJE on matrix metalloproteinase (MMP)-1 and MMP-3 expressions and the underlying molecular mechanism in UVB-irradiated primary human dermal fibroblasts (HDFs). The effect of TJE on HDF cell viability was determined using the XTT assay and cell counting. MMP-1 and MMP-3 expressions levels were measured by western blotting and real-time PCR analysis. Activations of mitogen-activated protein kinase (MAPKinase), nuclear factor-κB (NF-κB), and activator protein-1(AP-1) were measured by western blotting. Our results showed that TJE effectively reduced UVB-induced MMP-1 and MMP-3 protein and mRNA levels. Moreover, TJE significantly blocked the UVB-induced activation of MAPK (p38 and JNK) and transcription factors (NF-κB and AP-1), but not ERK. Taken together, our results suggest that the TJE inhibits UVB-induced MMP expressions in HDFs and its may be a potential agent for the prevention and treatment of skin photoaging.

호두복합추출물이 골수유래대식세포의 파골세포 분화에 미치는 효과

공해진(Hae Jin Kong),강재희(Jae Hui Kang),유화연(Hwa Yeon Ryu),이현(Hyun Lee) 한의병리학회 2019 동의생리병리학회지 Vol.33 No.3

The purpose of this study was to evaluate the inhibitory effects of Juglans regia complex extract(JCE) consisted of Juglans regia, Eucommia ulmoides, Eleutherococcus senticosus and Zingiber officinale on osteoclast differentiation. Cell toxicity test by using CCK-8, TRAP activity and TRAP positive multi-nucleated cell counting were performed to evaluate inhibitory effect on differentiation of osteoclast from bone marrow derived macrophages(BMMs) induced by receptor activator of nuclear factor-κB ligand(RANKL). As a result, JCE inhibited RANKL-induced osteoclast differentiation in BMMs dose-dependently without cytotoxicity. These results suggest that JCE may have a potential role for treating bone lytic diseases such as osteoporosis.

Ethical guidelines and regulations. etc

편집부(편집자) 한의병리학회 2015 동의생리병리학회지 Vol.29 No.4

아토피 피부염과 심리적 요인에 대한 동서의학적 고찰

노현민(Hyeon Min Noh),박승구(Sung Gu Park),조은희(Eun Hee Jo),장현철(Hyun Chul Jang),김호경(Ho kyoung Kim),박히준(Hee Jun Park),이길희(Gil Hee Lee),박민철(Min Cheol Park) 한의병리학회 2018 동의생리병리학회지 Vol.32 No.2

This study aimed to investigate the latest trends in the relationship between atopic dermatitis(AD) and psychological factors(PF) and to examine it in korean medicine. We searched MEDLINE for this analysis with the title “atopic dermatitis” AND (“psychology” OR “psychological” OR “mental” OR “emotion” OR “anxiety” OR “depression”) in recent 5 years and searched OASIS on the title “atopy” OR “psychology” OR “emotion” from 2002 to 2017. We selected 23 papers on MEDLINE, 7 papers on OASIS. In western medicine, Stress causes changes in the adrenal nerves, endocrine, and immunological mechanisms and exacerbates dermatitis, which is explained by HPA axis and sympathetic nerve axis, neurogenic inflammation, and cholinergic signals. In Korean medicine, Stress(神) exacerbates AD by affecting the five organs, especially the heart(心), causing inflammation(火熱). We studied the link between AD and PF in Western and Korean medicine. More research is needed in the future.

연산오계의 성별과 부위별 항염증 및 면역 활성 비교 연구

도영민(Young Min Do),김동희(Dong Hee Kim) 한의병리학회 2018 동의생리병리학회지 Vol.32 No.2

The aim of this study is to compare the anti-inflammatory and immunological activity of different parts (bone, meat, and rind) of Yeonsan Ogye (YO). In order to evaluate cytotoxicity, MTT assay was performed. We investigated the production of nitric oxide (NO) and pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α, in LPS-induced RAW264.7 cells. All parts of the YO showed no toxicity at concentrations of 1, 10, and 100 ㎍/㎖. Rooster"s bone, hen"s bone, and rind decreased the production of NO. And rooster"s bone, meat, and hen"s bone also attenuated TNF-α production in LPS-induced RAW 264.7 cells. In addition, all parts of the YO decreased IL-1β and IL-6 production in LPS-induced RAW264.7 cells, whereas they all increased IL-1β, IL-6 and TNF-α production in normal RAW264.7 cells. Rooster exhibited higher immune activation and inhibitory activity on inflammation than a hen, and among different parts of the YO, bone showed the highest activity. Our results demonstrated and compared the anti-inflammatory and immunological activity of different parts of the YO. These results suggest that YO may be developed as a raw material for new health supplement food and medicine to attenuate various symptoms related to inflammation and immunity.

Journal of Physiology & Pathology in Korean Medicine 외

편집부(편집자) 한의병리학회 2017 동의생리병리학회지 Vol.31 No.3

한의 오장 변증·평가 설문지 개발을 위한 신뢰도 및 타당도 평가

장은수(Eunsu Jang),김윤영(Yunyoung Kim),박양춘(Yang Chun Park),전주현(Ju Hyun Jeon),정인철(In-Chul Jung) 한의병리학회 2017 동의생리병리학회지 Vol.31 No.3

The aim of this study was to identify the reliability and the validity of Five Organs Questionnaire (FOQ). In this study, 335 data was collected from early adulthood of A-city, D-city and C-city in South Korea from Dec. 2016 to Jan. 2017, and the collected data was analyzed with SPSS 23.0 software. The result showed that the Cronbach’s α of five organs was 0.775-0.853. The reliabilities of test and retest Intra Correlation Coefficient (ICC) for the five organs were .891-.929 in pattern identification and .874-.930 in functional evaluation, respectively. Each organ was divided by two factors, and the factor loadings of Liver, Heart, Spleen, Lung and Kidney were 52.973%, 54.534%, 57.060%, 53.803%, and 46.337%, respectively. The functional evaluation of five organs was associated with self-rated health status as r was -.443 to -.583(p<.001), and quality of life as r was -.5.17 to -.716(p<.001), respectively. The test-retest diagnostic agreement was 67.2% and Kappa was 0.562. This study revealed that FOQ is a reliable and valid questionnaire.

蘇木과 그 지표물질인 brazilin이 인간 유래 각질 형성 세포의 tight junction 유전자 발현에 미치는 영향

천성혜(Seong Hye Cheon),최선경(Sun Kyung Choi),조남준(Nam Joon Cho),김기광(Kee Kwang Kim),이웅희(Woong Hee Lee),황형서(Hyung Seo Hwang),김균언(Kyoon Eon Kim),한효상(Hyosang Han) 한의병리학회 2018 동의생리병리학회지 Vol.32 No.2

The aim of this research was to determine the diverse effects of Sappan Lignum extract and brazilin on human keratinocyte HaCaT cells. We confirmed the antioxidant effect of Sappan Lignum extract and brazilin was analyzed by using an ABTS assay, confirming the efficacy of water extraction method. Also, we examined effect of Sappan Lignum extract and brazilin on the cell viability, using the MTS assay in HaCaT cells. mRNA expression levels of tight junction-related genes associated with skin barrier in HaCaT cells were analyzed using quantitative real-time PCR analysis. Sappan Lignum extract increased the cellular activity of HaCaT cells and the expression of the tight junction-related genes claudin 3, claudin 6, and ZO-2. Brazilin displayed the same effects as that of the extract on HaCaT cells activity and tight junction-related genes expression. Furthermore, dispase assay demonstrated altered cell–cell adhesion strength of Sappan Lignum extract or brazilin treated HaCaT cells. Sappan Lignum extract or brazilin might be an useful ingredient in skin-mosturizinng and anti-wrinkle cosmetics, given its effects of altering mRNA expression of tight junction-related genes and enhancing cell–cell adhesion strength of HaCaT cells.