사람태아골모세포에 대한 GBM의 골형성 유도에 관한 효과

최호철,최희인,유형근 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.2

The purpose of the present study was to investigate the effects of GBM on the activity, differentiation and alkaline phosphatase(ALP) synthesis of hFOB1s. To examine the cellular activity, hFOB1s were cultured with DMEM/F-12 1:1 Mixture and 100 ㎍, 2 mg, 5 mg, 10 mg of GBM for 2 days and 4 days. To compare the ALP synthesis, hFOB1s were cultured with DMEM/F-12 1:1 Mixture and 100 ㎍, 2 mg, 5 mg, 10 mg, 20 mg of GBM for 3days. The cellular activity of hFOB1s treated with 100 ㎍ of GBM was increased at 2 and 4-day(p>0.05) to control. The activity of ALP in hFOB1s treated with 100 ㎍ GBM was significantly increased at 3 day(p<0.05). This study indicated that 100 ㎍ of GBM has an inductive effect on bone formation in vitro increasing with cell proliferation, ALP activity.

鬱金이 Angiogenesis 抑制機轉에 미치는 影響

崔昇勳,安圭錫,成熙根 慶熙大學校韓醫科大學韓醫學硏究所 1999 慶熙韓醫大論文集 Vol.22 No.1

This experimental study was carried out to evaluate the effects of Curcuma on angiogenic inhibition mechanism. In order to investigate the effects of Curcuma on angiogenic inhibition mechanism evaluate cell survival rate by MTT assay, cell adhesive inhibition effect, DNA fragmantaion analysis. Nuclear Condensation assay FACScan analysis, angiogenesis inhibition assay. Immunocytochemistry analysis, RT-PCR for mRNA expression. Western Blot analysis. The results were summarized as follows : 1. The IC50 was 10㎍/ml to ECV 304 cell. 2. The cell adhesive inhibition ability was strong from 5㎍/ml. 3. The G0/G₁arrest peak was existed on ECV304 cell-line. 4. The cells on Collagen plate were inhibition of proliferation and inducement of apoptosis by Cur water extract. 5. Lumen formation was inhibited by Cur water extract. 6. LFA-1 and ELAM-1's expression were inhibited by Cur water extract, they are commenly participation on inflammation and tumor regeneration. 7. The expression of MMP-9 and uPA were inhibited by Cur water extract. 8. The expression of integrin αvβ₃ was inhibited by Cur water extract. 9. The expression of intracellular molecule were successively inhibited by Cur water extract therefore the proliferation of ECV304 cell line was stopped and apoptosis was induced. According to the results Curcuma has been shown to be a key antagonist of intergrin αvβ₃and which seems to be apoptosis by P53 through Flow cytometry. This report demonstrated that expression of MMP-9 and uPA blocked to under angiogenesis model. Thus, we suggested that Curcuma water block angiogenesis by inducing unscheduled apoptosis in ECV304 and ECVPAR cell lines and another orient herbal medicine that treat Qi-stagnation and Blood-stasis type also have angiogenic inhibition effects.

大學卒業定員制의 實施에 따른 學生生活 指導方案에 關한 硏究

崔根爀,張忠根,李相祐,張鎭吉,張大熙 충남대학교 1982 學生生活硏究 Vol.10 No.1

Higher education in Korea is faced with a new situation since 1981 when the government implemented a college graduation quota system designed to broaden the opportunities for higher education, alleviate the problem of the students who fail the entrance examination, and improve the quality of college graduates. The change of the system has brought about several serious problems including how to supply enough faculty members, how to provide enough educational facilities, and how to cope with the huge increase in the number of students, 30% more than graduation quota. The greatest single problem resulting from the implementation of college graduation quota system is the psychological and emotional unease aroused among the students, because 30% of those students with unsatisfying results must be banished from college before their graduation. This study aims to find effective measures for student guidance that each of the colleges can implement to help students adjust to the changed circumstances. After objectively investigating the problems from the various educational environments, from the recent students' views of college, and from the psychological and emotional unease arising in them by the fear of failing to graduate from their college because of implementation of the graduation quota system, a survey was conducted by means of a questionnaire composed of 40 questions and by means of personal interviews, 5,903 students, who are influenced by the graduation quota system, attending 19 public and private universities and colleges selected throughout the country. The data obtained from this survey were statistically processed with the help of the C. N. U. Computer Center, and the ratios of the responses to each of the questions were analyzed and calculated according to the distinctions of the total, sex, region, and major. The conclusions that the interrelations among the variant factors from each of the questions which were surveyed, analyzed and arranged have suggested are as follows : 1. The original purpose of graduation quota system should continually be clarified and communicated to all students and a concrete, specialized guidance system should be established to guide the minority of the students (13%) who don't fully understand the basic properties of higher educational institutions. 2. The institutions should provide enough educational facilities and supply enough faculty memebers to solve the problem of accomodating the large numbers of students. At the same time, they should suggest how students should learn positively and effectively to gain an expert knowledge, and how professors should gain public confidence in grade evaluation. Accompanying those measures, the government should develop a good policy to expand and distribute job opportunities to college graduates. 3. The admission quota should be controlled depending on the rate of naturally failed students, and then the students' unease and suppression caused by excessive competition can be relieved. In addition, it is advisable that the banished students should be allowed to transfer to University of the Air without regard to their grades. 4. An education devised to let students have a sound sense and way of thinking should be accompanied by a guidance system which their seniors, parents and society take part in. 5. An atmosphere conducive to sweet and well-rounded friendship among classmates should be created, sound extra-curricular activities should be encouraged and the number of students and adviser professor is in charge of should be lessened to a reasonable number.

인천 인현동 호프집 화재 피해자 분석

최정태,안무업,안희철,최영미,정재봉,서정열,유기철,이삼우,박석현,조준휘,김성환,김아진,송근정 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Purpose : This study was conducted to develop field triage, transportation, distribution, and prehospital care at a fire disaster by analyzing the victims of the fore that broke out at a bar in Incheon. Method : We analyzed the cases of the victims of a fire in Incheon in Oct. 1999. We determined the primary care hospital, the arrival time, the burn size, the outcome, and the injury type from the medical records, the concerned organ records, and interviews with concerned persons. Result : The total number of victims was 137: 56 prehospital deaths, 1 hospital death, and 80 survivals. The Pearson correlation coefficient between the burn size and the severity was -0.175. There were 121(89.6%) cases of inhalation injury, 59 (43.7%) cases of flame burns, 66 (48.9%) cases of hypoxic brain damage, and 16 (11.9%) cases involving other types of injury. Conclusion : The causes of death of the fire victims were inhalation injury and hypoxic brain damage due to CO poisoning and other toxic inhalants. We propose the use of a simple triage and rapid treatment(START) system and a reassessment the delayed category in fire disasters.

공간정보를 이용한 홍수재해지도 예측분석

임희창, 최석근 충북대학교 건설기술연구소 2013 충북대학교 건설기술연구소 논문집 Vol.32 No.1

본문참고

고령토로부터 알루미나 추출에 관한 연구(Ⅲ):염산에 의한 추출

최세영,이용근,이희수,김형국 연세대학교 산업기술연구소 1992 논문집 Vol.24 No.1

The preparation conditions of alumina powder extracted by hydrochloric acid from domestic San-Cheong kaolin have been investigated. In order to obtain best conditions to extract alumina by hydrochloric acid, experiments carried out with various acid concentrations, reaction temperatures, reaction times, and pH. The optimum conditions were 20 wt.-% for hydrochloric aicd concentration, 100℃ for reaction temperature, 7hrs for reaction time and pH8. The yield of alumina found to be 89.2wt.-% under these conditions. Two drying methods, direct and ethanol distillation drying method to control the precipitated agglomeration have deen compared. The ethanol distillation drying method revealed more narrow particle size distribution and less agglomerate than the direct drying method.

갑상선 유두선암 환자의 말초 혈액 단핵구로부터 수지상세포분화와 자가 세포독성 T 림프구 활성화 유도

송수근,최영식,박요한,이대희,장희경 고신대학교 의학부 2004 高神大學校 醫學部 論文集 Vol.19 No.1

Background: Dendritic cells (DCs) are rare leucocytes that are uniquely potent in their ability to capture, process and present antigens to T cells, and so selectively migrate through tissues to reach lymph nodes and spleen where initiation of immune responses takes place. It has been reported that a dense infilteration of dendritic cells correlates with a favorable prognosis in several types of cancer. A similar correlation also has been noted in patients with papillary carcinoma of thyroid. The purpose of this study is to determine whether DCs are generated from peripheral blood monocytes by using cytokines such as GM-CSF, TNF-α, and IL-4 and whether cytotoxic T cells activated by DCs attack the papillary carcinoma tissues. Methods: Peripheral blood was obtained from 2 patients with thyroid papillary cancer. DCs were established from monocytes by culturing in the presence of GM-CSF, Flt-3 ligand. TNF-α , and IL-4 for14 days. At day 14, expression of surface markers typical for DCs was analyzed morphologically. The immunophenotypic features of DCs such as CDla, CD83, and CD86 were analyzed by phase contrast microscopy. At day 15 DCs were incubated with thyroid cancer tissues and normal thyroid tissues for 7 days. Results: DCs were generated from the peripheral blood monocytes. The generated cells had the classic morphology of DCs. Activated cytotoxic T lymphocytes (CTLs) were observed. Veiled cell known as circulating DCs attached to the thyroid cancer tissues were observed. The CTLs activated by DCs were observed to attack the papillary carcinoma tissues by scanning electron microscope. However, normal tissues were not attacked by CTLs. Conclusion: DCs can be generated from the peripheral blood monocytes. Futhermore, the CTLs activated by DCs also can attack the papillary carcinoma tissues. These results that DCs can be used as adjuvants for adoptive immunotherapy of papillary thyroid cancer.

자일리톨 껌 저작에 의한 유치 우식증 예방효과 비교분석

한성근,최연희,손은영,송근배,김영진,남순현 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

자일리톨 껌 저작에 의해 어린이들의 유치우식증 예방효과를 평가하기 위하여 3개 어린이집에서 만 5~6세 어린이 123명을 최종 선정하였다. 자일리톨 껌을 씹는 군(42명), 솔비톨 껌을 씹는 군(42명) 그리고 아무런 조치도 취하지 않은 대조군(39명)으로 나누어 12개월 동안 하루 5회씩 껌을 저작하게 한 다음 구강검사와 우식활성 검사를 통해 유치우식증 예방효과를 비교 분석 하였다. 12개월 동안 대조군 어린이들에서는 dfs index가 5.19개면 증가하였고 솔비톨 군에서는 2.96개면이 증가한 반면 자일리톨 군에서 2.62개면이 증가하여 대조군에 비해 자일리톨 군은 47.1%가 감소하였고 솔비톨 군에서는 43.6%가 감소한 것으로 나타났다. Dentocult-SM strip 검사 및 site strip 검사 결과 모두에서 대조군에 비해 자일리톨 껌과 솔비톨 껌을 씹은 군의 아동들에서 우식활성도가 공히 감소되었다. 따라서 본 연구와 같은 집중적인 자일리톨 껌의 저작 사업은 대상자들과 부모들의 사업에 대한 호응도가 높았고, 사업 시행이 다른 국책 구강보건사업에 비해 용이하였으며 특히 유치우식증 예방적인 측면에서 효과적인 것으로 나타났다. The effect of xylitol and sorbitol chewing gums on dental caries levels among 5-year old kindergarteners was investigated. Three kindergartens the operations of which were partly subsidized by municipal government of Daegu city, Korea, were chosen as the study sites. The child base of the kindergartens were considered demographically and ethnically similar, representing middle-income families. The subjects were examined at their own kindergarten by the two dentists. Participation in the program was voluntary. Over 12 months, 123 participants chewed xylitol chewjng gums(X group; 42 subjects), sorbitol chewing gum@ group; 42 subjects), or did not receive chewing gum as a control group(C group: 39 subjects). Consumption of xylitol and sorbitol was 4.5 to 5.0 g/day/subject, consumed in five daily chewing episodes of 5 min. Oral examination, Dentocult-SM test and interproximal dental plaque collection were completed at baseline and 12 months later. The dmfs of group C increased 59.2%. but group S increased 33.4% and group X increased only 31.3% during 12 months study period. The caries prevention ratio was 47.1% at group X and 43.6% at group S. There also appeared the reduction of caries activity at group X(1.39), group S(1.50) than control group(1.79). Compared with groups S and C, there was a statistically significant reduction of S. mutans in interproximal plaque in group X. The results suggest that xylitol chewing gum can prevent dental caries of deciduous dentition, and may be a little more effective than a sorbitol-containing product in controlling some caries-associated parameters in kmdergarten-age subjects.

폐구리염화물용액의 분무열분해반응에 의한 생성분말의 특성에 관한 연구

박희범,최재권,한진아,유재근 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 연구의 목적은 폐 구리염화물 용액을 원료로 사용하여 분무열분해 공정에 의해 평균입도가 1 ㎛ 이하이며 입도분포가 균일하고 치밀한 조직을 나타내는 미립의 구리산화물 분말을 제조하는데 있다.또한 본 연구에서는 분무열분해 공정에 의해 생성되는 분말의 특성에 영향을 미치는 반응 온도, 원료용액의 유입속도, 분위기기체 및 공기의 유입속도, nozzle tip 크기 및 원료용액의 농도 등의 반응인자들의 영향을 검토하였다.

법랑질 접착에 대한 습윤 효과

고근호,조영곤,진철희,유상훈,김종욱,박병철,기영재,최희영,김종진 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.3

This study evaluated the microleakage and interfacial gap between enamel and composite resin under the dry and wet condition of the enamel surface, V shaped class 5 cavities were prepared on the occlusal portion of extracted human molars. Samples were divided into three groups: D group (air dry for 10-15s), BD group (blot dry with moist cotton pellet), and DR group (air dry for 10-15s and rewet with Aqua-Prep F for 20s). Cavities were filled using Aelitefil composite resin after applied One-Step. Microleakage was tested by 2% methylene blue dye solution and the data were statistically analysed by Kruskal-Wallis test and Mann-Whitney test. Also Enamel-resin interface was observed under SEM. Group BD showed statistically lower microleakage than group D (p〈0.05). but there was no statistically significant difference between group BD and DR (p)0.05). At the enamel-resin interface, group D showed the gap of 2 ㎛ thickness, but group BD and DR showed close adaptation. In conclusion, the use of blot dry and rewetting agent (Aqua-Prep F) resulted in decreased microleakage and improved adhesion between enamel and resin when using One-Step.