이종 탈회 건조골 및 Hydroxyapatite 제재의 골 이식이 성견 치조골 결손부 치유에 미치는 영향에 관한 비교연구

정성화,김종여,이재현 단국대학교 치의학연구소 1993 논문집 Vol.5 No.-

This study was conducted to evaluate the healing potential of hydroxyapatite and demineralized freeze dried bone in 5 dogs. Chronic periodontitis was induced by ligating elastic wire randomized as follows. The group in which only flap operation was performed was used as control. The group in which flap operation using nonresorbable nonporous hydroxyapatite (Orthomatrix) was performed was used as experimental Ⅰ. The group which flap operation using resorbable porous hydroxyapatite (Biocoral) was performed as experimental Ⅱ. The group in which flap operation using demineralized freeze-dried bone was performed was used as experimental Ⅲ. Thereafter dogs serially sacrificed at the 1,2,4 and 8 weeks and the specimens were prepared, and stained with Hematoxilin-Eosin stain for the light microscopic evaluation. The results of the this study were as follows : 1. Control group : progressive inflammatory cell infiltration till 4 weeks and epithelial undergrowth. 2. Group Ⅰ : epithelial undergrowth and new bone formed with fibrous margin around HA granule. 3. Group Ⅱ : no epithelial undergrowth and direct bone formation at the porous granule 4. Group Ⅲ : could not see epithelial undergrowth but obviously new cementum formation.

탈지대두박에서의 Isoflavone의 분리를 위한 흡착제의 조건

여경은,조성빈,김우정 한국산업식품공학회 2003 산업 식품공학 Vol.7 No.4

탈지대두박을 이용한 isoflavone의 분리 및 정제를 위한 최적 조건을 찾고자 absorption resin인 Amberlite XAD-1180를 사용하여 흡착제의 활성화용매의 농도, 흡착된 isoflavone의 용출용매의 농도, 용출용매의 양과 용출속도, 시료와 흡착제의 비율 그리고 수지에 미치는 pH와 ion의 영향을 조사하였다. 수지의 활성화와 용출에는 Amberlite XAD-1180의 경우 methanol 75%로 활성화시키기 ethanol 80%로 용출시키는 경우가 가장 높은 값을 보였으며, 이때 사용하는 absorption resin은 isoflavone 흡착을 위해 사용한 단백질제거 탈지대두박용액양의 약 0.5배로, ethatnol의 양은 단백질제거 탈지대두박용액 양의 4배를 사용하여 flow rate 12mL/min의 경우가 적절한 조건임이 밝혀져 흡착제 사용이 isoflavone 함량을 최고 2~3배까지 향상시키는 결과를 보였다. 이때 분리된 isoflavone의 순도는 최고 40%까지 얻을 수 있었다. pH와 ion의 영향에서는 pH는 5.0,NaCl은 0.25N 이하에서 isoflavone의 유출량이 높았다. Conditions for sorption and elution of isoflavone with using amberlite XAD-1180 were studied to separate Isoflavone from defatted soybean flour (DSF). Isoflavone analyzed were daidzin, genistin, glycitin, daidzein, genistein and glycitein by HPLC. The result showed that washing Amberlite XAD-1180 with 75% methanol and then elution of isoflavone with 80% ethanol resulted the highest amounts of isoflavone recovered from the absorption resin. The most proper eluent factor was 4(eluent/sample volume), the sample factor was about 2(the volum of sample/the weight of resin) ad flow rate 12 mL/min. The pH 5.0 and less than 0.25 N NaCl were found to be proper for seperation of isoflavone. The isoflavone contents after seperation with absorption resin were about 40% in dry weight basis.

당뇨병 환자에서 발생된 고열을 동반한 베체트병 1예

김여주,오정은,김상진 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Behcet's disease is a multisystem vascular inflammatory disease of unknown origin. It is characterized by recurrent aphthous stomatitis, uveitits, genital ulcer and skin lesions. All these common manifestations are self-limiting except for the ocular attack. Repeated attacks of uveitis can cause blindness. Involvement of the gastrointestinal tract, central nervous system and large vessels is less frequent, although it can be life-threatening. There is no pathognomonic laboratory test but there are clinical criteria to assist in establishing the diagnosis. One of the clinical features of Behcet's disease is low-grade fever, which usually accompanies the inflammatory manifestations of the disease. But high fever is rare. Therefore, we reported a case of Behcet's disease presented by high fever in a patient with diabetes mellitus.

환경친화성 계면활성제를 이용한 어병균의 살균효과

최상원,정관용,오남희,김은영,방정환,김정우,여문환 한국환경과학회 2000 한국환경과학회지 Vol.9 No.5

The antibacterial effect amino acid-copper(Ⅱ) surfactant on fish pathogens was studied. Fish pathogens of Edwardsiella tarda, Vibrio anguillarum, Aeromonas hydrophila and Streptococcus sp. were selected, cultured in nutrient agar and adjusted at 2 × 10 exp (5)∼10 exp (6) CFU/㎖ in phosphate buffer saline before the addtion of amino acid-copper(Ⅱ) surfactant with different concentrations. All tested pathogens died within 1 hour with 1 ppm of amino acid-copper(Ⅱ) surfactant. In comparison with formalin and ET, amino acid-copper(Ⅱ) surfactnat was more effective in antibacterial capacity.

패혈증 백서 모델에서 비타민-C의 효과

이중의,신상도,표창해,서길준,정성은,윤여규 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Background : Multi-organ failure from sepsis is very lethal disease entity, which is suspected to be caused by activated inflammatory cells. Inflammatory cells activated by endotoxins generate oxidants and cytokines such as TNF-α and IL-6, which in turn stimulate macrophages and neutrophils. Augmented inflammation makes an organ-injury deteriorate into an organ-failure, which may progress to multi-organ failure. This study is designed to evaluate the therapeutic effects of vitamin-C, a scavenger of oxidants, in sepsis. Methods : Male Sprague-Dawley rats were divided into 3 groups : a control group, a group injected intrapetoneally with LPS(lipopolysaccharide), and a group injected intraperitoneally with LPS and vitamin-C. Each eight rats were sacrified 24 hours and 48 hours after injection, and samples of the blood, the liver and the lung were obtained. Biochemical assays of TNF-α level in the blood and malondialdehyde(MDA)level, catalase activity and nitric oxide synthase(NOS)activity in the liver and the lung tissues were performed. Results : Serum TNF-α level, tissue lipid peroxidation and tissue i-NOS activity were dramatically increased, and tissue catalase activity was exhausted rapidly in sepsis. High dose vitamin-C administration decreased serum TNF-α level, tissue lipid peroxidation and tissue i-NOS induction, and protected against catalase exhaustion. Conclusion : high dose vitamin-C therapy was proved to have definite antioxidant effect in septic condition.

동종 골수이식을 시행받은 환자에서 폐색성 세기관지염에 의하여 발생한 자발성 기종격동과 피하 기종

이병환,이제중,이연경,안재숙,김여경,황호인,박무림,조상희,정익주,김형준 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

저자들은 만성골수성백혈병으로 동종 골수이식과 이식편 부전으로 인하여 추가적인 말초혈액 조혈모세포이식을 시행 받은 환자에서 만성 이식편대숙주질환과 그 폐 합병증인 폐색성 세기관지염에 동반된 자발성 기종격동과 피하 기종이 병발한 1예를 경험하였기에 이를 보고하는 바이다. Obstructive lung disorders following after allogeneic bone marrow transplantation (BMT) in association with graft- versus-host disease (GVHD) contribute significant morbidity and mortality. We report a case of a 28-year-old man who developed spontaneous pneumomediatinum and subcutaneous emphysema complicating bronchiolitis obliterans after allogeneic BMT. He received an allogeneic BMT for chronic phase of chronic myeloid leukemia. Five months after BMT, he was boostered by allogeneic peripheral blood stem cells from the same donor due to graft failure. One month after the boostering, chronic GVHD developed and were treated with cyclosporine and steroid. The patients developed spontaneous pneumomediatinum and subcutaneous emphysema secondary to severe bronchiolitis obliterans 4 months after boostering donor cells. The air-leak syndromes were recovered by conservative management, including high-flow oxygen.

동종 골수이식 후 만성 이식편대숙주질환과 동반된 그레이브스병

황호인,이제중,조상희,김여경,이병환,정익주,김형준 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1

저자들은 중증 재생불량성빈혈로 동종 골수이식 및 추가적인 동종 말초혈액 조혈모세포이식을 시행 받은 후 만성 이식편대숙주질환이 발생하여 면역억제제로 조절되었으나 자가면역성 갑상선기능항진증이 병발한 1예를 경험하였기에 이를 보고하는 바이다. Chronic graft-versus-host disease (GVHD), which is a frequent complication following bone marrow transplantation (BMT), is characterized by fibrosis of the skin, liver, lungs, and gastrointestinal tract, immunodeficiency, and the production of autoantibodies. Endocrine dysfunction, in particular thyroid disease, has been described following BMT, but this has previously meant hypothyroidism secondary to total body irradiation or transfer of autoimmune hyperthyroidism from a donor with Graves’ disease rather than in association with chronic GVHD. We report a case of a 26-year-old man who developed Graves’ disease eight years after BMT and who had remission of chronic GVHD. He received an allogeneic BMT for severe aplastic anemia. Eighteen months after BMT, diarrhea and chronic GVHD developed and were treated with cyclosporine and steroid. Four years after ceasing the cyclosporine, the patient developed weight loss, palpitations, fever, and anterior neck swelling, and was diagnosed with Graves’ disease based on typical thyroid function tests. The signs and symptoms in the patient improved with propylthiouracil and propranolol.

치아회분과 석고혼합제재 매식과 자가골 동시 이식후 치유과정에 관한 실험적 연구

김영균,김흥중,이상호,여환호,임성철,설인택,정재헌 大韓顎顔面成形再建外科學會 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.1

The purpose of this study was to evaluate the efficacy of adding autogenous bone to the toothash-plaster mixture in the healing process of bone. Full-thickness round osseous defects with the diameter of 20mm were made at the calvarial bone of adult dogs (n=19) bilaterally, which were thought to be critical size defect. The right defects were repaired with the toothash-plaster mixture plus autogenous bone (compressed volume 0.3cc) and the left defects with only toothash-plaster mixture. At 2-, 4-, 8-, 12- and 20- week after implantation, dogs were sacrificed and evaluated the osseous healing of bony defects clinically, radiographically, and microscopically. The results were as follows : 1. At the clinical observation, the wound healed very well without any problem except severe swelling in the early period after operation. Slight depression was recognized at the both sides when the portions of cranial defect were palpated. 2. There were statistically significant differences between toothash-plaster mixture groups and autogenous bone added groups at the same period, and among the groups in the bone density of the digital radiograms (P<0.001).There was a tendency that bone density was increasing with time. 3. In light microscopic examination, new bone formation was more active in the autogenous bone added groups than toothash-plaster mixture groups at the early period after mplantation but there is little difference at 20-week after implantation. 4. In fluorescent microscopic examination, the fluorescent band could be observed at the area of active bone formation and the band was more distinct in the autogenous bone added groups then toothash-plaster mixture groups. 5. In transmitted electron microscopic examination, organelles such as rER, Golgi complex and secretory granule and osteoblast were observed. In summary higher volume ratio of autogenous bone is needed to improve the bone healing in that there is little difference between toothash-plaster mixture group and autogenous bone added group at the 20-week after implantation in spite of new bone formation was more active in the autogenous bone added groups than toothash-plaster mixture groups at the early period after operation.

STI571 (Glivec^(�))에 의하여 빠른 치료 반응을 보인 불응성 Gastrointestinal stromal tumor

윤경원,이제중,김여경,황호인,이병환,조상희,정익주,김형준 全南大醫大 2001 전남의대학술지 Vol.37 No.4

저 유전상수 시클로헥산계 플라즈마 고분자 박막의 특성

최자영,권영춘,여상학,정동근 성균관대학교 기초과학연구소 1999 論文集 Vol.50 No.-

In this work, we report on the investigation of the properties of cyclohexane-based plasma polymers (CHexPP) as functions of the deposition pressure and the plasma power. As the deposition pressure was decreased from 2 torr to 0.2 ton with a fixed plasma power of 60W, k value of the CHexPP thin film increased from 2.42 to 3.24. CHexPP thin films deposited at lower deposition pressure showed very good thermal stability, even the one deposited at 0.2 torn was stable up to 400℃. As the plasma power was increased from 10W to 90W with a fixed deposition pressure of 0.2 ton, k value increased from 2.61 to 3.39. The CHexPP thin film deposited at 90W were stable up to 450℃. All the deposited films were insulating. Under applied fields less than 1 MV/cm, leakage current densities were less than 10^-7 A/㎠.