Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

고관절 전치환술 후 합병된 비구 컵 해리와 동반된 활액낭종 : 증례보고 Case Report

성일보,정국진,박수철,김진호,정형진,황재광,염재광,정병현,안종국 대한고관절학회 2001 Hip and Pelvis Vol.13 No.1

Salmonella Typhimurium DT104

정현진,김의종 대한화학요법학회 1998 대한화학요법학회지 Vol.16 No.4

環境要因이 育雛에 미치는 影響에 關한 硏究 : 第1報 햇볕 照射가 育雛에 미치는 效果 Ⅰ. Effect of Sunlight on Chick Brooding

鄭鉉丞,朴成振,郭鍾瀅 진주산업대학교 1968 論文集 Vol.2 No.-

本 試驗은 環境要因中 햇볕조사가 育雛에 미치는 영향을 究明하기 爲하여 試驗에 着手하였던 바 다음과 같은 결과를 얻었다. 1.增體率에 있어서는 處理區間에 1% 水準의 高度의 有意性이 있었고 孵化 1週後에 放射한 T_1區가 C, T_2, T_3區보다 有意性이 높았다. 2.飼料의 要求率에 있어서 T_1區가 0.353으로서 C區(0.327), T_2區(0.309), T_3區(0.312)보다 높았다. 3.斃死率은 T_1區=0%, C區=5% T_2區=15% T_3區=10%였다. 以上의 結果로서 햇볕조사는 ??化後 1週부터 實施하는 것이 效果가 있음을 認定할 수 있다. This experiment was conducted to determine the effect of sunlight belonging to the environmental factor on chick brooding. the results obtained were as follows; 1.There were observed a significant difference (p<0.01)for body weight gain between treatments. T_1 lot which received sunlight after 1 weeks from hatching showed more body weight gain than that of C. lot, T_2 lot and T_3 lot. 2.In feed conversion T_1 lot with 0.353 was higher than C. lot (0.327), T_2 lot (0.309) and T_3. lot (0.312). 3.There was a mortality of 0% in T_1 lot, 5% C lot, 15% in T_2 lot and 10% in T_3 lot. From the above mentioned results of this experiment it is effective to expose the growing chick to sunlight after 1 weeks from hatching.

피압수가 존재하는 연약점토지반 내에 연직배수재의 관입깊이에 따라 압밀거동에 미치는 영향

정성교,진현식,백승훈 동아대학교 건설기술연구소 1999 硏究報告 Vol.22 No.2

In the Yangsan area developed for housing complex the soft clay deposited as about 20 to 40 meters in thickness and there was the Artesian pressure of about 20kPa under the clay stratum. To investigate the consolidation behaviour due to partially or fully penetrating vertical drains in the clay layer, a finite element analysis was carried out in this study. As a result of analysis, it was shown that the consolidation rate and the final settlement for the case of fully penetrating vertical drains were faster and less than of partially penetration, respectively.

상수도관의 노후도에 대한 환경영향인자의 상관성 분석

정원식,이현동,김이태,유명진 대한상하수도학회 2003 상하수도학회지 Vol.17 No.2

This study is performed to evaluate the correlation of environmental factors for drinking water pipe deterioration using statistical analysis such as multiple regression, cluster analysis, discriminant analysis, and the adequacy and dependability of grouping factors used to deterioration prediction model. Results of this study are presented that high correlation related to pipe deterioration is showed not laying year of pipe but characteristic of surrounding area and analysis of chemical components. Therefore, major influence factors of pipe deterioration for types of cast iron pipe are product quality of pipe, environmental factors using pipe deterioration evaluation model is classified to 4 types such as group related to pipe body and to hydraulic & water quality, and to characteristics of surrounding area, and to trouble & discontent of customer. And the very high adequacy of group classification is represented that the applied grouping items in this study are agree to previous studies.

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

역행성 골수강내 금속정을 이용한 대퇴골 과상-과간 골절의 치료

정필현,황정수,강 석,김종필,전용진 대한골절학회 2004 대한골절학회지 Vol.17 No.2

새로운 알랄아민 항진균제의 합성과 생물학적 평가

정병호,박은주,문현주,유진철 朝鮮大學校 1997 藥學硏究誌 Vol.18 No.2

Some allylamine compounds which are benzothiazole substituants in stead of naphthylring in naftifine, antifungal agents, were sythesized as potential antimycotics. The intermediate Schiff bases that were obtained by condensation of 2-aminobenzothiazole and trans-cinnamaldehyde, were reduced to imine compounds to give allylamines(5a-5d) after mrthylation. These compounds which were tested in vitro against five fungal cell lines containing Trichophyton mentagrophytes. showed no activity in 0.1∼100 μg/ml range.

창조적 지식 기반 사회를 대비한 국어과 교육의 목표와 내용

정혜승,노명완,옥현진 이화여자대학교 교육과학연구소 2000 교육과학연구 Vol.31 No.1

본고는 창조적 지식 기반사회에서 가장 중요한 국어과 교육의 목표는 언어 사용 기능의 신장이며, 이를 실현시키기 위한 교육 내용에 대해 논의하였다. 일부 국어교육학계에서 기능은 하찮고 저열한 것으로 인식되기도 하였으나, 이는 지식의 개념을 명제적 지식에 국한하여 생각하고 기능을 지식과 별개의 것으로 잘못 인식한 데서 기인한 것이다. 본고는 명제적 지식과 방법적 지식의 관계를 탐구하여 기능 학습에 관한 이론을 두 가지로 정리하였다. 하나는 명제적 지식을 우선하여 이를 연습하고 내재화하는 가운데 기능 학습이 이루어진다고 보는 것이며, 다른 하나는 명제적 지식을 전제하지 않고 다양한 경험과 활동을 통해 기능 학습이 이루어진다는 것이다. 전자는 학습 활동이 용이하고 체계적이라는 점에서, 후자는 앎의 과정을 보다 중시한다는 점에서 장점을 갖고 있으나, 한 편으로 전자는 명제적 지식의 성격이 기능 학습에 유효한 것이어야 한다는 검과, 후자는 실제 수업이 이루어지기 위해서 치밀한 계획과 준비가 필요하다는 점에서 문제점도 안고있었다. 3차 국어과 교육과정은 후자의 방법을, 6차와 7차 국어과 교육과정은 전자와 같은 인식을 보이고 있으나, 논의가 개념 수준에서 이루어져 언어 사용 기능 교육이 제대로 이루어지기 어렵다는 한계를 갖는다. 따라서 국어과 교육의 논의는 개념 수준에서 벗어나 실시간과 실공간 속에서 학생들이 무엇을 어떻게 배울 것인지를 구체적으로 제시하는, 곧 수업화ㆍ자료화하는 실질적이고도 기술적인(technical) 논의로 바뀌어야 한다고 본다. Aim and Content of Korean Language Education for a Society Based on Creative Knowledge This thesis argues the improvement of language use skill that is the most important aim of Korean language education in a society based on creative knowledge and the education contents to achieve it. Some Korean language educators regard skill as worthlessness and vulgarity. However it results from the misunderstanding that the concept of knowledge is limited to knowing that and that skill is entirely different from knowledge. This paper summarizes the theory on skill study in two ways by researching the relations between knowing that and Know-how. One is that skill study is done in the process of exercising and internalizing knowing that preferentially and the other is that it's done through a lot of experiences and actives without any premise of knowing that. These have respective merits : former has the easiness and systematic of skill study and the latter thinks highly of the processing of knowledge. On the contrary, there are problems : in case of the former, the content of knowing that should be effective to skill study and the latter needs accurate plan and preparation for actual study. The 3rd education course in Korean language with latter method and the 6th and 7th without former on place a limit that the argument only in concept level makes the actual language use skill difficult. Therefore the argument of Korean language education should be changed to actual and technical one to present in detail what and how students learn in real time and real space.