Predicting the Duration of a General Contracting Industrial Project based on the Residual Modified Model

Jian-Xia Guo,Chang-Ming Hu,Rong Bao 대한토목학회 2019 KSCE Journal of Civil Engineering Vol.23 No.8

The general contracting projects are developed in China, in the past two decades. In the early stage of a project, because of the lack of specific design drawings and project plans, it is difficult for the general contractor to determine the construction period when signing the contract. However, little research has undertaken on quickly and efficiently estimating construction duration of a general contracting industrial project. Therefore, the purpose of this paper is to explore a suitable model for estimating construction duration of the general contracting industrial project in China. Data for 90 completed projects are collected in a company that undertakes nationwide industrial projects. Four single variable models and fourteen multivariate models are analyzed using statistical method. And the residual modified model integrating wavelet neural network (WNN) is also developed through using a predictive error to amend the statistical model. The results show that the residual modified models obtain more enhanced prediction accuracy than regression models, despite their good fitting performance. The modified model can be used for helping contractors forecast project duration in the early stage of a project.

Tristetraprolin Overexpression in Gastric Cancer Cells Suppresses PD-L1 Expression and Inhibits Tumor Progression by Enhancing Antitumor Immunity

Guo, Jian,Qu, Huiheng,Shan, Ting,Chen, Yigang,Chen, Ye,Xia, Jiazeng Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.7

The RNA-binding protein tristetraprolin (TTP) binds to adenosine-uridine AU-rich elements in the 3'-untranslated region of messenger RNAs and facilitates rapid degradation of the target mRNAs. Therefore, it regulates the expression of multiple cancer and immunity-associated transcripts. Furthermore, a lack of TTP in cancer cells influences cancer progression and predicts poor survival. Although the functions of TTP on cancer cells have previously been researched, the mechanism of TTP on the interaction between cancer cells with their micro-environment remains undiscovered. In this study, we admed to determine the role of cancer cell TTP during the interaction between tumor and immune cells, specifically regulatory T cells (Tregs). We evaluate the capability of TTP to modulate the antitumor immunity of GC and explored the underlying mechanism. The overexpression of TTP in GC cells dramatically increased peripheral blood mononuclear lymphocyte (PBML) -mediated cytotoxicity against GC cells. Increased cytotoxicity against TTP-overexpressed GC cells by PBMLs was determined by Treg development and infiltration. Surprisingly, we found the stabilization of programmed death-ligand 1 (PD-L1) mRNA was declining while TTP was elevated. The PD-L1 protein level was reduced in TTP-abundant GC cells. PD-L1 gas been found to play a pivotal role in Treg development and functional maintenance in immune system. Taken together, our results suggest the overexpression of TTP in GC cells not only affects cell survival and apoptosis but also increases PBMLs -mediated cytotoxicity against GC cells to decelerate tumor progression. Moreover, we identified PD-L1 as a critical TTP-regulated factor that contributes to inhibiting antitumor immunity.

Long-term Stable Field Electron Transfer from Carbon Nanotube Arrays at High Emission Current Densities

Jian-hua Deng,Zhao-xia Ping,Rui-ting Zheng,Guo-an Cheng 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.58 No.41

The stability behaviors of multiwalled carbon nanotube arrays during field electron emission are studied. The results indicate that the stability, even at a high emission current density, has been greatly improved by an aging process, and a degradation of about 0.66% in the emission current density at 21.86 mA/cm_2 during a 10-hour stability test has been obtained. A detailed analysis of the deterioration of the field electron emission characteristics is given, and the generation of Joule heat during field emission is found to be able to burn off the extruded carbon nanotubes, which will directly reduce the number of emission sites. On the other hand, the Joule heating effect may induce an annealing of the defects existing in the carbon nanotubes and may influence the distribution of electron energy states, both having a bad influence on the field emission characteristics. An aging process, especially aging at high emission current densities, can greatly reduce the influence of Jouleheating induced current degradation. Hence, an aging process at high emission current densities provides an effective way to realize long-term stable field electron emission from carbon nanotube arrays.

Tristetraprolin Overexpression in Gastric Cancer Cells Suppresses PD-L1 Expression and Inhibits Tumor Progression by Enhancing Antitumor Immunity

Jian Guo,Huiheng Qu,Ting Shan,Yigang Chen,Ye Chen,Jiazeng Xia 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.7

The RNA-binding protein tristetraprolin (TTP) binds to adenosine- uridine AU-rich elements in the 3’-untranslated region of messenger RNAs and facilitates rapid degradation of the target mRNAs. Therefore, it regulates the expression of multiple cancer and immunity-associated transcripts. Furthermore, a lack of TTP in cancer cells influences cancer progression and predicts poor survival. Although the functions of TTP on cancer cells have previously been researched, the mechanism of TTP on the interaction between cancer cells with their microenvironment remains undiscovered. In this study, we admed to determine the role of cancer cell TTP during the interaction between tumor and immune cells, specifically regulatory T cells (Tregs). We evaluate the capability of TTP to modulate the antitumor immunity of GC and explored the underlying mechanism. The overexpression of TTP in GC cells dramatically increased peripheral blood mononuclear lymphocyte (PBML) -mediated cytotoxicity against GC cells. Increased cytotoxicity against TTP-overexpressed GC cells by PBMLs was determined by Treg development and infiltration. Surprisingly, we found the stabilization of programmed death-ligand 1 (PD-L1) mRNA was declining while TTP was elevated. The PDL1 protein level was reduced in TTP-abundant GC cells. PD-L1 gas been found to play a pivotal role in Treg development and functional maintenance in immune system. Taken together, our results suggest the overexpression of TTP in GC cells not only affects cell survival and apoptosis but also increases PBMLs -mediated cytotoxicity against GC cells to decelerate tumor progression. Moreover, we identified PD-L1 as a critical TTP-regulated factor that contributes to inhibiting antitumor immunity.

An Overlooked Effect of Glycine Betaine on Fermentation: Prevents Caramelization and Increases the L-Lysine Production

( Jian Zhong Xu ),( Xiu Hua Xia ),( Jun Lan Zhang ),( Yan Feng Guo ),( Wei Guo Zhang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.10

This article focuses on the effects of glycine betaine on preventing caramelization, and increasing DCW and L-lysine production. The additional glycine betaine not only decreased the browning intensity (decreased 4 times), and the concentrations of 5-hydroxymethylfurfural (decreased 7.8 times) and furfural (decreased 12 times), but also increased the availability of glucose (increased 17.5%) for L-lysine production. The DCW and L-lysine production were increased by adding no more than 20 mM glycine betaine, whereas the DCW and L-lysine production were decreased with the reduction of pH values, although pH had a better response to prevent caramelization than did glycine betaine. For L-lysine production, the highest increase (40%) was observed on the media with 20 mM glycine betaine. The crucial enzymes in glycolysis and L-lysine biosynthesis pathway were investigated. The results indicated that additional glycine betaine increases the activity of enzymes in glycolysis, in contrast to the effect of pH. All the results indicated that glycine betaine can be used to prevent caramelization and increase the L-lysine production. By applying this strategy, glucose would not be have to be separated from the culture media during autoclaving so that factories can save production costs and shorten the fermentation period.

Isolation and Preliminary Identification of a Novel Microorganism Producing Aspartame from Soil Samples

Jian Dong Cui,Ya Nan Zhang,Gui Xia Zhao,Shi Ru Jia,Guo Qun Zhao,Si Zhang,Jun Lu 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.2

To develop a new method for producing aspartame (APM), a simple and efficient strategy for the isolation of certain microorganisms producing APM from soil samples was designed. A newly strain with secreting certain specific dipeptidase to directly synthesize APM from the substrates of L-aspartic acid (L-Asp) and Lphenylalanine methyl ester (L-PM) without protection for amino acid side chains was screened from soil samples. APM concentration in reaction mixture was quantified by high performance liquid chromatography (HPLC), yield reached 0.015 g/L. Examination of the general morphological characteristics and data from the sequence analysis of the rDNA-ITS gene led to identification of the isolate as a strain of fungal endophyte spp. The newly isolated strain had a high potential for application in industrial processes for APM production. In particular, this new method was low cost for synthesis of APM during the reaction due to avoiding protection for amino acid side chains and optical resolution of the mixtures. As we known it, this is first report that a newly strain with a high potential for selective synthesis of the APM was isolated from soil.

Fabrication of polymer compound microlens by lens-on-lens microstructures

Zehua Xia,Yan Li,Xiaoya Su,Yanhua Han,Zhongyi Guo,Jian-Min Gao,Qiaoqun Sun,Shiliang Qu 한국물리학회 2017 Current Applied Physics Vol.17 No.1

We proposed a novel method for fabricating polymer compound microlenses (PCMLs) using micro-inkjet technique and subsequent curing process. Two different types of PCMLs with sandwich microstructure (PDMS-Glycerol-PDMS), concave and convex PCMLs, have been designed and fabricated in experiments. Convex PCML has two real images and two foci. The concave PCML has one real and one virtual focal planes, which can generate one real image and one virtual image respectively. Moreover, the diameter of concave PCML can be controlled by adjusting the curing time and temperature. The proposed method is simple, efficient and suitable for realizing large-scale high numerical aperture PCMLs array, which has potential applications in diverse optical systems such as optical storage and three-dimensional imaging.

Inspirations for China`s Cultural Industry Development from the Construction of Korea`s Cultural Industry Chain

( Ping Jian Guo ),( Hai Xia Fang ) 복식문화학회 2010 Fashion, industry and education Vol.13 No.2

The purpose of this research was to understand the successful establishment of the cultural industry chain in Korea and discover lessons for China to improve its cultural industry. It was concluded that a one-industry development pattern cannot win in market competition and a cultural industry will strengthen its sustainability only through smoothing its relationship with other industries and establishing a cultural industry chain so as to further development and resist crises together.

Analysis of Geographic and Pairwise Distances among Chinese Cashmere Goat Populations

Liu, Jian-Bin,Wang, Fan,Lang, Xia,Zha, Xi,Sun, Xiao-Ping,Yue, Yao-Jing,Feng, Rui-Lin,Yang, Bo-Hui,Guo, Jian Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3

This study investigated the geographic and pairwise distances of nine Chinese local Cashmere goat populations through the analysis of 20 microsatellite DNA markers. Fluorescence PCR was used to identify the markers, which were selected based on their significance as identified by the Food and Agriculture Organization of the United Nations (FAO) and the International Society for Animal Genetics (ISAG). In total, 206 alleles were detected; the average allele number was 10.30; the polymorphism information content of loci ranged from 0.5213 to 0.7582; the number of effective alleles ranged from 4.0484 to 4.6178; the observed heterozygosity was from 0.5023 to 0.5602 for the practical sample; the expected heterozygosity ranged from 0.5783 to 0.6464; and Allelic richness ranged from 4.7551 to 8.0693. These results indicated that Chinese Cashmere goat populations exhibited rich genetic diversity. Further, the Wright's F-statistics of subpopulation within total (FST) was 0.1184; the genetic differentiation coefficient (GST) was 0.0940; and the average gene flow (Nm) was 2.0415. All pairwise FST values among the populations were highly significant (p<0.01 or p<0.001), suggesting that the populations studied should all be considered to be separate breeds. Finally, the clustering analysis divided the Chinese Cashmere goat populations into at least four clusters, with the Hexi and Yashan goat populations alone in one cluster. These results have provided useful, practical, and important information for the future of Chinese Cashmere goat breeding.

High-accuracy electrohydraulic control system for the position and orientation of the primary mirror for a large telescope

Li Yu-Xia,Wang Jian-Li,Guo Peng-Fei,Li Hong-Wen,Cao Yu-Yan 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.7

Due to variations in gravity, temperature, and external disturbances, the optical axes of a telescope’s primary mirrors can shift and, consequently, become misaligned with the light path. Such misalignment significantly reduces image quality; thus, the position and orientation of primary mirror (POPM) control systems must maintain the optical axis in an ideal position. Therefore, in this paper, we proposes a novel high-accuracy electrohydraulic control system for the position and orientation of the primary mirror (POPM) of a large telescope. To this end, a POPM control system with five electrohydraulic partitions is adopted, and a mathematical model of the POPM is derived. In addition, a mathematical model of each partition of the electrohydraulic system is derived for the telescope controller design. A linear active disturbance rejection controller (LADRC) and a sliding mode controller (SMC) are adopted in each electro-hydraulic partition to ensure positioning accuracy. Experiments are carried out on 4 and 1.2 m large telescopes. The corresponding results show that by both keeping constant and varying the elevation of the large telescopes, the position error of the primary mirror can be limited to less than 1 μm, and the orientation of the primary mirror can be maintained with an error of less than 0.05 arcsec, even in the presence of external disturbances. This control accuracy can guarantee both the inalterability of the optical axis of the primary mirror and the possibility to adjust the light according to the requirement in order to obtain high-resolution images.