The Subjective Reflection and Artificial Intelligence Imagination of Sci-Fi Movies in the Post-Human Era

Jian-Dong, Cui,Seung-keun Song 한국콘텐츠학회 2021 한국콘텐츠학회 ICCC 논문집 Vol.2021 No.12

With the rapid development of disruptive technological achievements such as biotechnology, artificial intelligence, simulation technology, and genetic engineering, it has approached and even threatened the boundary and pattern of human existence, and is deeply challenging the subjectivity of natural persons. With the development of technology and virtual technology, the hybrid of organisms and machines (agents)-Cyborg (cyborg) is gradually consistent with humans both physically and consciously. This phenomenon has caused people to reflect on human subjectivity. The concretized representation of “cyborg”, artificial intelligence, and virtual space-time in post-human theories in science fiction movies involves the development of human form, social structure, and the future world. It shows how humans coexist with other life forms and helps We think about the living conditions of people and the relationship between people and the world in the “post-human” era.

Optimal culture condition for the production of phenyalanine ammonia lyase from E. coli

Jian-dong Cui,Yan Li 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.2

The effects of culturing conditions on phenylalanine ammonia lyase production by a recombinant E. coli strain were investigated by using a controlled fed-batch fermentation system. In a 5 L fermentor, the optimal composition of the batch medium was 2% glucose, 1% yeast extract, 0.7% K2HPO4, 0.8% KH2PO4, 0.5% (NH4)2SO4, 0.1% MgSO4·7H2O. The optimal feed glucose solution was 50%. Glucose concentration and pH of the culture broth were maintained at about 2.0 g/L and 7.0 during the fed-batch phase, respectively. Following 24-h cultivation, 0.2 mmol/L isopropyl-β-D-thiogalactopyranoside (IPTG) was added and temperature was shifted from 37 oC to 42 oC to induce pal gene expression. Under optimal conditions, a high productivity of 300 U/g could be achieved after 48 h culture, and a cell density of OD600 about 82 was obtained at 52 h culture at 500 r/m stirrer speed and 1 vvm, respectively.

로봇 저널리즘 연구 동향 및 미래 전망

Cui, Jian-Dong,Song, Seung-keun 한국정보통신학회 2020 한국정보통신학회논문지 Vol.24 No.2

AI-powered robot news is drawing attention as artificial intelligence technology is fully spread in the news distribution field. Robot news still has many technical and ethical problems, but academic research on this is insufficient. This study analyzes the issue of robot writing in artificial intelligent based robot journalism industry using SWOT analysis. As a result, the advantages of big data processes, accurate information gathering, high efficiency and disadvantages such as lack of independent arguments and lack of evidence and opportunities for technical development, government support, academic development, and industrial applications, and threats such as uncritical acceptance and lack of talent have been found. This study suggests three future-oriented directions, such as human-machine collaboration, intelligent news, and chat-bot, through previous studies on the development direction of robot journalism-based article writing.

Enhanced Activity of Phenylalanine Ammonia Lyase in Permeabilised Recombinant E. coli by Response Surface Method

Jian-dong Cui,Yan Li,Shi Ru Jia 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.2

To improve phenylalanine ammonia lyase (E.C.4.3.1.5-PAL) activity in recombinant Escherichia coli, Some approaches for improving phenylalanine ammonia lyase (PAL) activity in recombinant E. coli were developed following preliminary studies by means of response surface method. The results shown that permeabilization with combination of Triton X-100, cetyl trimethyl ammonium bromide (CTAB), and acetone enriched cellular recombinant PAL activity significantly, which improved over 10-fold as compared with the control (untreat cell), as high as 181.37 U/g. The optimum values for the tested variables were Triton X-100 0.108 g/ℓ, CTAB 0.15 g/ℓ, and acetone 45.2%(v/v). Furthermore, a second-order model equation was suggested and then validated experimentally. It was indicated that addition of surfactants and organic solvents made the cells more permeable and therefore allowed easier access of the substrate to the enzyme and excretion of the product, which increased the rate of transport of L-phenylalanine and trans-cinnamic acids. These improved methods of PAL activity enrichment could serve as a rich enzyme source, especially in the biosynthesis of L-phenylalanine.

Optimization of medium for phenylalanine ammonia lyase production in E. coli using response surface methodology

Jian-Dong Cui 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.1

A culture medium for phenylalanine ammonia lyase (PAL) production in E. coli was developed following preliminary studies by means of response surface methodology (RSM). The medium components having significant effect on the production were first identified by using a fractional factorial design. Then, central composite design (CCD)was used to optimize the medium constituents and explain the combined effects of four medium constituents: glucose,yeast extract, (NH4)2HPO4 and MgSO4. A quadratic model was found to fit the PAL production. CCD revealed that the optimum values of the test variables for PAL production were glucose 28.2 g/L, yeast extract 5.01 g/L, (NH4)2HPO47.02 g/L and MgSO4 1.5 g/L. PAL production of 62.85 U/g, which was in agreement with the prediction, was observed in the verification experiment. In comparison to the production of basal medium, 1.8-fold increase was obtained.

Isolation and Preliminary Identification of a Novel Microorganism Producing Aspartame from Soil Samples

Jian Dong Cui,Ya Nan Zhang,Gui Xia Zhao,Shi Ru Jia,Guo Qun Zhao,Si Zhang,Jun Lu 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.2

To develop a new method for producing aspartame (APM), a simple and efficient strategy for the isolation of certain microorganisms producing APM from soil samples was designed. A newly strain with secreting certain specific dipeptidase to directly synthesize APM from the substrates of L-aspartic acid (L-Asp) and Lphenylalanine methyl ester (L-PM) without protection for amino acid side chains was screened from soil samples. APM concentration in reaction mixture was quantified by high performance liquid chromatography (HPLC), yield reached 0.015 g/L. Examination of the general morphological characteristics and data from the sequence analysis of the rDNA-ITS gene led to identification of the isolate as a strain of fungal endophyte spp. The newly isolated strain had a high potential for application in industrial processes for APM production. In particular, this new method was low cost for synthesis of APM during the reaction due to avoiding protection for amino acid side chains and optical resolution of the mixtures. As we known it, this is first report that a newly strain with a high potential for selective synthesis of the APM was isolated from soil.

Influence of Controlled- and Uncontrolled-pH Operations on Recombinant Phenylalanine Ammonia Lyase Production in Escherichia coli

Jian Dong Cui,Gui Xia Zhao,Ya Nan Zhang,Shi Ru Jia 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.4

Effects of controlled- and uncontrolled-pH operations on phenylalanine ammonia lyase (PAL) production by a recombinant Escherichia coli strain were investigated at uncontrolled-pH (pHUC) and controlled-pH (pHC) of 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, and 8.5 in bioreactor systems. The results showed that the recombinant PAL activity was improved significantly by controlled pH strategy. Among the pHC operations, the highest PAL activities were obtained under pHC 7.5 strategy where cell mass (OD600 ㎚) and PAL activity was 1.3 and 1.8 fold higher than those of pHUC, respectively. The maximum PAL activity reached 123 U/g. The pHC 7.5 strategy made recombinant plasmid more stable and therefore allowed easier expression of PAL recombinant plasmid, which increased PAL production. It was indicated that the new approach (controlled-pH strategy) obtained in this work possessed a high potential for the industrial production of PAL, especially in the biosynthesis of Lphenylalanine.

A facile technique to prepare cross-linked enzyme aggregates of bovine pancreatic lipase using bovine serum albumin as an additive

Jian Dong Cui,Rong Lin Liu,Lin Bo Li 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.2

Cross-linked enzyme aggregates from bovine pancreatic lipase were prepared by co-aggregation of lipase and BSA (Lipase-BSA-CLEAs). The main factors in the preparation of lipase-BSA-CLEAs were optimized. The highest activity recovery was around 75% under the condition of using 1% (v/v) glutaraldehyde as cross-linker and 0.05 g/L bovine serum albumin as feeder for 2 h cross linking. The optimum temperature for both lipase-CLEAs and lipase- BSA-CLEAs was measured as 60 oC, which is 10 oC higher than that of free lipase. Moreover, the lipase-BSA-CLEAs evidenced higher thermal stability and excellent reusability in comparison with the lipase-CLEAs. Lipase-BSA-CLEAs retained more than 75% of the initial activity after eight cycles of reuse, while lipase-CLEAs only retained 20% of its initial activity. Additionally, lipase-BSA-CLEAs showed more storage stability than free lipase and lipase-CLEAs. The high stability and recyclability of lipase-BSA-CLEAs make it efficient for different industrial applications.

Optimization of Medium on Exopolysaccharides Production in Submerged Culture of Cordyceps militaris

Jian-Dong Cui,Shi-Ru Jia 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.6

Statistical experimental design strategy (SES) was applied to optimize the medium for the exopolysaccharides (EPS) production of Cordyceps militaris by submerged culture in shaker flask. A significant influence of the glucose and peptone on the EPS production was first evaluated by using a Plackett-Buman design. Then,steepest ascent method was employed to approach the experimental design space. Last, these factors were further optimized using central composite design (CCD) and response surface methodology (RSM). A quadratic model was found to fit the EPS production. The optimum values of the tested variables for the production of EPS were 48.67 g/L glucose, 12.56 g/L peptone, 1 g/L KH2PO4, 10 g/L yeast extract, and 0.5 g/L MgSO4·7H2O. Under optimization of culture conditions, the EPS production was enhanced from 0.78 to 1.96 g/L. In comparison with that of original culture conditions, 2.5 fold increase was obtained.

새로운 시대의 교육에서 AI 교육 로봇의 응용 효과에 대한 메타 분석

추이지엔동(Jian-Dong, Cui),송승근(Seung-keun Song) 한국정보통신학회 2021 한국정보통신학회 종합학술대회 논문집 Vol.25 No.1

인공 지능 시대가 도래함에 따라, 로봇 교육과 그 역량 강화에 대한 교육은 전 세계적으로 널리 적용되고 적용되었습니다. 본 연구의 목적 : 학생 교육 및 교육에서 AI 교육 로봇의 응용 효과를 체계적으로 평가; 본 연구의 방법 : 컴퓨터를 사용하여 "Web of Science", "CNKI", "ERIC", "IEEE"와 같은 검색 도구에서 관련 교육을 검색하십시오. 로봇 티칭과 전통적인 티칭의 효과에 대한 비교 연구 검색시간은 2000 년 1 월부터 2020 년 1 월까지입니다. 포괄적 인 MetaAnalysis 2.0을 메타 분석에 사용했습니다. 본 연구 결과 : 31 개의 유효한 연구 문헌에 대한 정량 분석과 메타 분석이 AI 교육 로봇에 미치는 영향에 대한 객관적인 평가 분석 결과 AI 교육 로봇이 학생 학습 효과에 미치는 효과는 0.465임 이는 교육용 로봇이 학생의 학습 효과에 어느 정도 긍정적 인 영향을 미침을 나타냅니다. 이 연구의 결론 : 학생 교육 및 교육에서 AI 교육 로봇의 적용 효과는 전통적인 교육 방법보다 우수하며 학생 학습을 보다 효과적으로 홍보 할 수 있습니다. With the advent of the era of artificial intelligence, robot education and teaching under its empowerment have been widely concerned and applied worldwide. The purpose of this study: systematically evaluate the application effect of AI educational robots in student education and teaching; the method of this study: use the computer to search for relevant education in the search tools such as "Web of Science", "CNKI", "ERIC", "IEEE" A comparative study of the effects of robot teaching and traditional teaching. The retrieval time is from January 2000 to January 2020. Comprehensive MetaAnalysis 2.0 was used for Meta analysis. The results of this study: A quantitative analysis of the 31 valid research literatures included, and an objective evaluation of the effect of the meta-analysis on AI educational robots. The analysis results show that the combined effect of AI educational robots on student learning effects is 0.465 This indicates that educational robots have a moderately positive effect on students ’learning effectiveness. The conclusion of this study: The application effect of AI educational robots in student education and teaching is better than traditional education methods, which can better promote student learning.