Effect of Portal Vein Chemotherapy on Liver Metastasis after Surgical Resection of Colorectal Cancer

Yu, Dong-Sheng,Li, Ying,Huang, Xin-En,Lu, Yan-Yan,Wu, Xue-Yan,Liu, Jin,Cao, Jie,Xu, Xia,Xiang, Jin,Wang, Guo-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objective: To explore the effect of portal vein chemotherapy on liver metastasis after surgical resection of colorectal cancer. Methods: Patients fulfilling the eligibility criteria were assigned to receive either surgery plus 1-week continuous infusion of 5-FU (study group) or surgery alone (observational group). Patients in the study group received portal vein chemotherapy, whereby 5-FU (1000 mg/d) and heparin (5000 IU/d) infusion was initiated from the day of surgery and lasted for 7 consecutive days. Liver metastasis was monitored during five years follow-up postoperatively. Results: Sixty four patients were recruited and assigned to the study group (12 with colon and 20 with rectal cancer) or the control group (10 with colon and 22 with rectal cancer). Liver metastasis rate was 12.5% in study and 25.0% in observational group, the difference being significant (P<0.01). Conclusion: Portal vein chemotherapy could be an effective treatment in preventing liver metastasis after surgical resection of colorectal cancer.

Effects of prolonged photoperiod on growth performance, serum lipids and meat quality of Jinjiang cattle in winter

Yu, Yan,Qiu, Jingyun,Cao, Jincheng,Guo, Yingying,Bai, Hui,Wei, Shengjuan,Yan, Peishi Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9

Objective: This study was conducted to investigate the potential effects of prolonged photoperiod on the serum lipids, carcass traits, and meat quality of Jinjiang cattle during winter. Methods: Thirty-four Jinjiang bulls aged between 14 and 16 months were randomly assigned to two groups that were alternatively subjected to either natural daylight +4 h supplemental light (long photoperiod, LP) or natural daylight (natural photoperiod, NP) for 96 days. The potential effects on the levels of serum lipids, carcass traits, meat quality, and genes regulating lipid metabolism in the intramuscular fat (IMF) of the cattle were evaluated. Results: Jinjiang cattle kept under LP showed significant increase in both dry matter intake and backfat thickness. the serum glucose and the plasma leptin levels were significantly reduced, while that of melatonin and insulin were observed to be increased. The crude fat contents of biceps femoris muscle and longissimus dorsi muscle were higher in LP than in NP group. In longissimus dorsi muscle, the proportions of C17:0 and C18:0 were significantly higher but that of the C16:1 was found to be significantly lower in LP group. The relative mRNA expressions in IMF of longissimus dorsi muscle, the lipid synthesis genes (proliferator-activated receptor gamma, fatty acid-binding protein) and the fatty acid synthesis genes (acetyl-coa carboxylase, fatty acid synthetase, 1-acylglycerol-3-phosphate acyltransferase) were significantly up-regulated in LP group (p<0.05); whereas the hormone-sensitive lipase and stearoyl-CoA desaturase 1 were significantly down-regulated in LP than in NP group. Conclusion: Prolonged photoperiod significantly altered the growth performance, hormonal levels, gene expression and fat deposition in Jinjiang cattle. It suggested that the LP improved the fat deposition by regulating the levels of different hormones and genes related to lipid metabolism, thereby improving the fattening of Jinjiang cattle during winter.

The purified extract of steamed Panax ginseng protects cardiomyocyte from ischemic injury via caveolin-1 phosphorylation-mediating calcium influx

Hai-Xia Li,Yan Ma,Yu-Xiao Yan,Xin-Ke Zhai,Meng-Yu Xin,Tian Wang,Dong-Cao Xu,Yu-Tong Song,Chun-Dong Song,Cheng-Xue Pan 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6

Background: Caveolin-1, the scaffolding protein of cholesterol-rich invaginations, plays an important rolein store-operated Ca2þ influx and its phosphorylation at Tyr14 (p-caveolin-1) is vital to mobilize protectionagainst myocardial ischemia (MI) injury. SOCE, comprising STIM1, ORAI1 and TRPC1, contributesto intracellular Ca2þ ([Ca2þ]i) accumulation in cardiomyocytes. The purified extract of steamed Panaxginseng (EPG) attenuated [Ca2þ]i overload against MI injury. Thus, the aim of this study was to investigatethe possibility of EPG affecting p-caveolin-1 to further mediate SOCE/[Ca2þ]i against MI injury in neonatalrat cardiomyocytes and a rat model. Methods: PP2, an inhibitor of p-caveolin-1, was used. Cell viability, [Ca2þ]i concentration were analyzedin cardiomyocytes. In rats, myocardial infarct size, pathological damages, apoptosis and cardiac fibrosiswere evaluated, p-caveolin-1 and STIM1 were detected by immunofluorescence, and the levels ofcaveolin-1, STIM1, ORAI1 and TRPC1 were determined by RT-PCR and Western blot. And, release of LDH,cTnI and BNP was measured. Results: EPG, ginsenosides accounting for 57.96%, suppressed release of LDH, cTnI and BNP, and protectedcardiomyocytes by inhibiting Ca2þ influx. And, EPG significantly relieved myocardial infarct size, cardiacapoptosis, fibrosis, and ultrastructure abnormality. Moreover, EPG negatively regulated SOCE viaincreasing p-caveolin-1 protein, decreasing ORAI1 mRNA and protein levels of ORAI1, TRPC1 and STIM1. More importantly, inhibition of the p-caveolin-1 significantly suppressed all of the above cardioprotectionof EPG. Conclusions: Caveolin-1 phosphorylation is involved in the protective effects of EPG against MI injury viaincreasing p-caveolin-1 to negatively regulate SOCE/[Ca2þ]i.

Anti-VEGF Therapy with Bevacizumab - Limited Cardiovascular Toxicity

Yu, Jing,Cao, Xu-Fen,Zheng, Ye,Zhao, Rong-Cheng,Yan, Li-Qiu,Zhao, Lei,Wang, Jia-Wang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Purpose: This analysis was conducted to evaluate cardiovascular toxicity of commonly used anti-VEGF therapeutic agent, bevacizumab, in treating patients with cancer. Methods: Clinical studies evaluating the efficacy and safety of bevacizumab-based regimens on response and safety for patients with cancer were identified using a predefined search strategy, allowing cardiovascular toxicity and other side effects of treatment to be estimated. Results: In bevacizumab based regimens, 4 clinical studies including 282 patients with advanced cancer (including gliomas, cervical, breast and ovarian cancer) were considered eligible for inclusion. These bevacizumab-based regimens included docetaxel, irinitecan and carboplatin. Systematic analysis suggested that, of 282 patients treated by bevacizumab based regimens, hypertension and thrombo-embolism occurred in 2.5% (7/282), while only 3 patients reported cardiovascular events (1.1%). No treatment related death occurred in bevacizumab based treatment. Conclusion: This systemic analysis suggests that bevacizumab based regimens are associated with reasonable and accepted cardiovascular toxicity when treating patients with gliomas, cervical, breast and ovarian cancer.

Lysinibacillus tabacifolii sp. nov., a Novel Endophytic Bacterium Isolated from Nicotiana tabacum Leaves

Yan-Qing Duan,Song-Tao He,Qing-Qing Li,Ming-Feng Wang,Wen-Yuan Wang,Wei Zhe,Yong-Hong Cao,Ming-He Mo,Yu-Long Zhai,Wen-Jun Li 한국미생물학회 2013 The journal of microbiology Vol.51 No.3

A Gram-positive, catalase- and oxidase-positive, strictly aerobic, endospore-forming rod bacterium, designated K3514T, was isolated from the leaves of Nicotiana tabacum. The strain was able to grow at temperatures of 8–40°C, pH 5.0–10.0 and NaCl concentrations of 0–7%. The predominant quinones (>30%) of this strain were MK-7(H2) and MK-7. Phylogenetic analysis of 16S rRNA gene sequence showed that strain K3514T was affiliated to the genus Lysinibacillus, with its closest relatives being Lysinibacillus mangiferihumi (98.3% sequence similarity), Lysinibacillus sphaericus (97.9% sequence similarity), Lysinibacillus fusiformis (97.4% sequence similarity), and Lysinibacillus xylanilyticus (97.3% sequence similarity). However, low levels of DNA-DNA relatedness values suggested that the isolate was distinct from the other closest Lysinibacillus species. Additionally, based on analysis of morphological, physiological, and biochemical characteristics, the isolate could be differentiated from the closest known relatives. Therefore, based on polyphasic taxonomic data, the novel isolate likely represents a novel species, for which the name Lysinibacillus tabacifolii sp. nov. and the type strain K3514T (=KCTC 33042T =CCTCC AB 2012050T) are proposed.

Analysis of leaking ratio of sealing device for the intake port of EMC closed integral structure turbine disk through soft computing

Cao, Yan,Zandi, Yousef,Rahimi, Abouzar,Fan, Qingming,Bai, Yu,Guo, Junde,Fu, Leijie,Khadimallah, Mohamed Amine,Jameel, Mohammed,Assilzadeh, Hamid Techno-Press 2021 Advances in nano research Vol.11 No.3

The rotor disk produces an azimuthal velocity component in the space between the rotor and stator of a turbine disk, also known as the rim seal cavity. As part of an empirically study, the rim seal cavity is subjected to a test aimed to count the number of unstable structures and evaluate their rotational speed. An Electronic Control Unit (ECU) has a leak test aperture that is sealed by a sealing device that is selectively disposed in the leak test aperture. Designing pump and compressor machines and units requires the development of dependable seal assemblies that maintain tightness over a long period of time and in a wide variety of pressures and temperature. In the field of electrochemical machining (ECM), heat-resistant and high-strength materials may be machined into complicated forms using this well-known technology. ECM presents some issues as the electrochemical copying of grooves, insulating groove features, slots and mini-holes can cause water leakage due to the poor sealing device of the closed integral structure turbine disk. Sealing devices of rotor turbine disks are heavy components with low-cycle fatigue analysis to their life curves. However, there is rare analysis to detect their defects in various rotor regions (temperature, considering stress, mission profile). This study by use of hydro-thermal loading has attempted to focus on the mechanical seals rings and basic productive and operating requirements. Taking the damage and wear, the clearance has been altered that cause a raising in leakage. Generally, the leakage grows more rapidly than linearly with the after-damage clearance. Also, damage and wear were related to the labyrinth seal itself, resulting that the bending curvature and the percentage of bent tooth length were also relevant in defining the leakage in the case of bending damage.

Alternative Messenger RNA Splicing of Autophagic Gene Beclin 1 in Human B-cell Acute Lymphoblastic Leukemia Cells

Niu, Yu-Na,Liu, Qing-Qing,Zhang, Su-Ping,Yuan, Na,Cao, Yan,Cai, Jin-Yang,Lin, Wei-Wei,Xu, Fei,Wang, Zhi-Jian,Chen, Bo,Wang, Jian-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Beclin 1 is a key factor for initiation and regulation of autophagy, which is a cellular catabolic process involved in tumorigenesis. To investigate the role of alternative splicing of Beclin1 in the regulation of autophagy in leukemia cells, Beclin1 mRNA from 6 different types of cell lines and peripheral blood mononuclear cells from 2 healthy volunteers was reversely transcribed, subcloned, and screened for alternative splicing. New transcript variants were analyzed by DNA sequencing. A transcript variant of Beclin 1 gene carrying a deletion of exon 11, which encoded a C-terminal truncation of Beclin 1 isoform, was found. The alternative isoform was assessed by bioinformatics, immunoblotting and subcellular localization. The results showed that this variable transcript is generated by alternative 3' splicing, and its translational product displayed a reduced activity in induction of autophagy by starvation, indicating that the spliced isoform might function as a dominant negative modulator of autophagy. Our findings suggest that the alternative splicing of Beclin 1 might play important roles in leukemogenesis regulated by autophagy.

Regulatory effect of trehalose metabolism on chitin synthesis in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) as determined using RNAi

Yang Yan-qi,Liang Yu-jian,Zhi Junrui,Li Ding-yin,Li Cao 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1

Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) is an important pest of crops that causes huge losses to grain production. Trehalose plays an important regulatory role in chitin synthesis. In this study, the mRNA expression of the trehalose synthase (TPS), soluble trehalase (TRE1), and membrane-bound trehalase (TRE2) genes in S. frugiperda were silenced by RNAi to reveal the mechanism by which trehalose metabolism regulates chitin synthesis. The results showed that the survival rate of S. frugiperda at different developmental stages decreased in varying degrees following RNAi, and abnormal phenotypes were detected. TRE1 activity was extremely significantly inhibited after dsTRE1 and dsTRE2 injection, and TRE2 activity was extremely signifi cantly inhibited after dsTPS and dsTRE2 injection. After silencing different trehalose metabolism-related regu latory genes, the trehalose content increased, whereas the glucose content decreased. Chitin synthase A (CHSA) gene was upregulated at 24 h after dsTPS and dsTRE1 injection. CHSA expression was significantly inhibited at 48 h after dsTRE1 injection. Chitin synthase B (CHSB) gene expression was significantly higher at 24 h after dsTRE1 injection. Furthermore, CHSB expression was significantly inhibited at 48 h after dsTPS, dsTRE1, or dsTRE2 injection. RNAi of TRE1 or TRE2 led to a significant decrease in chitin content. The results suggested that inhibiting the mRNA expression of genes related to trehalose metabolism in S. frugiperda could disrupt normal trehalose metabolism, thereby regulating the mRNA expression of downstream chitin synthase genes and further affecting chitin synthesis.

High-accuracy electrohydraulic control system for the position and orientation of the primary mirror for a large telescope

Li Yu-Xia,Wang Jian-Li,Guo Peng-Fei,Li Hong-Wen,Cao Yu-Yan 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.7

Due to variations in gravity, temperature, and external disturbances, the optical axes of a telescope’s primary mirrors can shift and, consequently, become misaligned with the light path. Such misalignment significantly reduces image quality; thus, the position and orientation of primary mirror (POPM) control systems must maintain the optical axis in an ideal position. Therefore, in this paper, we proposes a novel high-accuracy electrohydraulic control system for the position and orientation of the primary mirror (POPM) of a large telescope. To this end, a POPM control system with five electrohydraulic partitions is adopted, and a mathematical model of the POPM is derived. In addition, a mathematical model of each partition of the electrohydraulic system is derived for the telescope controller design. A linear active disturbance rejection controller (LADRC) and a sliding mode controller (SMC) are adopted in each electro-hydraulic partition to ensure positioning accuracy. Experiments are carried out on 4 and 1.2 m large telescopes. The corresponding results show that by both keeping constant and varying the elevation of the large telescopes, the position error of the primary mirror can be limited to less than 1 μm, and the orientation of the primary mirror can be maintained with an error of less than 0.05 arcsec, even in the presence of external disturbances. This control accuracy can guarantee both the inalterability of the optical axis of the primary mirror and the possibility to adjust the light according to the requirement in order to obtain high-resolution images.

Alkaloids from Lycoris caldwellii and their particular cytotoxicities against the astrocytoma and glioma cell lines

Peng Cao,Dong-Sheng Pan,Song Han,Chun-Yong Yu,Qing-Jie Zhao,Yan Song,Yong Liang 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.8

Phytochemical investigation of the ethanolextract of the bulbs of Lycoris caldwellii afforded fournew alkaloids, (?)-N-methoxylcarbonyl-nandigerine (1),(?)-N-methoxycarbonyl-lindcarpine (2), (?)-10-O-methylhernovineN-oxide (3), and (?)-3-hydroxy-anhydrolycorineN-oxide (4). Structural elucidation of all the compounds wereperformed by spectral methods such as 1D and 2D (1H-1HCOSY, HMQC, and HMBC) NMR spectroscopy, in additionto high resolution mass spectrometry. All the alkaloids werein vitro evaluated for their cytotoxic activities against eighttumor cell lines (BEN-MEN-1, CCF-STTG1, CHG-5, SHG-44, U251, BGC-823, HepG2, and SK-OV-3). Alkaloids 1and 2 exhibited particular cytotoxic activities against astrocytomaand glioma cell lines with IC50 of 9.2–11.3 lM and10.4–12.2 lM respectively.