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- 저자 : Yanhua Han (검색결과 6 건)
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Heterologous Expression and Purification of Zea mays Transglutaminase in Pichia pastoris
Hongbo Li,Yanhua Cui,Lanwei Zhang,Huaxi Yi,Xue Han,Yuehua Jiao,Ming Du,Rongbo Fan,Shuang Zhang 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5
Transglutaminases (TGases) are a family ofenzymes that catalyze the cross-linking of proteins and arewidely used in the food industry to improve the texture ofdairy, meat, and bread products. Zea mays transglutaminase(TGZ) is a new type of TGase with a wide potential. TGZwas expressed in the yeast Pichia pastoris under an alcoholoxidase promoter. Maximal expression of recombinantTGZ was achieved by inducing recombinant GS115(pPIC9K-tgz) in BMMY medium using 1.5% methanol for96 h. Secreted TGZ was initially separated using Superdex200 resin and further purified on cation exchange resin. The activity of TGZ following purification was 0.32 U/mgof protein. The polymerization effect of TGZ on caseincatalyzed by recombinant TGZ was slightly lower than theeffect of microbial transglutaminase (MTG). TGZ is a newpotential additive for the food industry.
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Go, Yun Young,Li, Yanhua,Chen, Zhenhai,Han, Mingyuan,Yoo, Dongwan,Fang, Ying,Balasuriya, Udeni B. R. Hindawi Publishing Corporation 2014 BioMed research international Vol.2014 No.-
<P>The objective of this study was to investigate the effect of equine arteritis virus (EAV) on type I interferon (IFN) production. Equine endothelial cells (EECs) were infected with the virulent Bucyrus strain (VBS) of EAV and expression of IFN-<I><I>β</I></I> was measured at mRNA and protein levels by quantitative real-time RT-PCR and IFN bioassay using vesicular stomatitis virus expressing the green fluorescence protein (VSV-GFP), respectively. Quantitative RT-PCR results showed that IFN-<I><I>β</I></I> mRNA levels in EECs infected with EAV VBS were not increased compared to those in mock-infected cells. Consistent with quantitative RT-PCR, Sendai virus- (SeV-) induced type I IFN production was inhibited by EAV infection. Using an IFN-<I><I>β</I></I> promoter-luciferase reporter assay, we subsequently demonstrated that EAV nsps 1, 2, and 11 had the capability to inhibit type I IFN activation. Of these three nsps, nsp1 exhibited the strongest inhibitory effect. Taken together, these data demonstrate that EAV has the ability to suppress the type I IFN production in EECs and nsp1 may play a critical role to subvert the equine innate immune response.</P>
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Zou Yi,Changyou Han,Fang Wang,Yanhua Tan,Saina Yang,Chuying Huang,Shengyou Xie,Xueqin Xiao 한국식물학회 2021 Journal of Plant Biology Vol.64 No.2
Aloe vera L. is an excellent resource for medication. Selenium-enriched aloe can act as a functional food to human health. To understand the molecular mechanisms underlying selenium accumulation in Aloe vera L., we characterized the metabolic and transcriptome responses of aloe leaves under different Na2SeO4 levels (0, 200, and 400 mg/L) treatments. Aloe leaves spraying with exogenous selenium fertilizer showed a significant increase in total Se content compared with those under non-treatment control, and no distinct differences were observed between 200 and 400 mg/L Se treatment. Non-targeted metabolic profiling revealed that Se treatment triggered the accumulation of antioxidants, including amino acid and derivatives, phenols, flavonoids, terpene, as well as indole derivatives. Consistent with metabolic changes following Se treatment, the transcript level of genes involved in Se assimilation and Se-response showed dramatically increase, such as those encoding sulfate transporter, antioxidants, phytohormone signaling, transcription factors, and phenols metabolites, suggesting Se assimilation generally accompanied with antioxidant and pathogen defense. This study exhibited comprehensive insights on Se response in Aloe vera L., and provided us with targeted genes for genetic engineering, thereby improving the therapeutic value of aloe.
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Fabrication of polymer compound microlens by lens-on-lens microstructures
Zehua Xia,Yan Li,Xiaoya Su,Yanhua Han,Zhongyi Guo,Jian-Min Gao,Qiaoqun Sun,Shiliang Qu 한국물리학회 2017 Current Applied Physics Vol.17 No.1
We proposed a novel method for fabricating polymer compound microlenses (PCMLs) using micro-inkjet technique and subsequent curing process. Two different types of PCMLs with sandwich microstructure (PDMS-Glycerol-PDMS), concave and convex PCMLs, have been designed and fabricated in experiments. Convex PCML has two real images and two foci. The concave PCML has one real and one virtual focal planes, which can generate one real image and one virtual image respectively. Moreover, the diameter of concave PCML can be controlled by adjusting the curing time and temperature. The proposed method is simple, efficient and suitable for realizing large-scale high numerical aperture PCMLs array, which has potential applications in diverse optical systems such as optical storage and three-dimensional imaging.
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Wei Wenying,Wang Zhen,Yin Yanhua,Han Jinyu,Xu Wen 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.3
Composition Group Vector Space (CGVS) method for estimating melting and boiling point Tm, Tb of organic compound has been proposed, and the principle of this method has been elucidated. The models for estimating Tm, Tb have been established and the numerical values of relative parameters have been presented. The average percentage deviations of Tm, Tb estimation are 7.53 and 1.58, respectively, which show that the present method demonstrates significant improvement in applicability to predict the above properties, compared to conventional group methods
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Jing Wu,Xintong Wang,Li Wu,Ya-ni Lu,Yanhua Han 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.8
As tunnelling progresses into the complex geological environment such as fault zones, water inrush has become one of the main geological hazards during tunnel construction. Consequently, understanding the evolution of pore pressure and flow velocity when a tunnel is excavated in a fault zone is crucial to ensure safe working conditions and reduce construction risks. In this work, based on the concept of “Three Zones” fault structure, we simulate the nonlinear water inrush process by solving the Darcy-Brinkman flow equation for the host rock and the fault zone. We examine the impacts of 1) the angle between the tunnelling direction and the fault and 2) the relative position from the tunnel face to the fault on the evolution of pore pressure and flow velocity near the tunnel face. The results show that within 5 m to 20 m ahead of the working face, pore pressure, flow velocity, and water inrush rate are the smallest when the angle is 90°. As the angle decreases, both pore pressure and flow velocity ahead of the working face increase. The pore pressure is larger when the tunnel has not reached the fault zone than when the tunnel has crossed the fault zone. Flow velocity also exhibits similar behaviour as pore pressure. With different relative positions from the tunnel working face to the fault, the closer the tunnel face to the fault, the lower the pore pressure and the larger the flow velocity ahead of the tunnel face. The largest water inrush rate occurs when the tunnel face is excavated to the center of the fault core, and the water inrush rate declines as the distance away from the fault increases. The simulation results provided a new method for simulating water inrush when a tunnel crosses a fault and could provide valuable references for the prediction of water inrush for underground projects.
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