소아의 급성 인두염에서 연쇄구균 신속항원검사의 유용성

임기현 ( Ki Hyun Lim ),최우진 ( Woo Jin Choi ),김미진 ( Mi Jin Kim ),김영호 ( Young Ho Kim ),정지아 ( Ji A Jung ),양승 ( Seung Yang ),황일태 ( Il Tae Hwang ),이혜란 ( Hae Ran Lee ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2006 소아알레르기 및 호흡기학회지 Vol.16 No.1

목 적 : 최근 소아 급성 인두염에서 부적절한 항생제 투여가 문제시 되고 있으나 증상만으로 항생제 사용 여부를 결정하기는 어려우며 인두배양검사가 확진 방법이다. 그러나 검사에 시간이 요구되므로 미국소아과학회에서는 배양검사와 신속항원검사를 시행하여 신속항원검사가 음성이면 항생제 사용을 보류하도록 권하고 있다. 저자들은 이와 같은 지침을 국내에서도 적용할 수 있는지 알아보고자 연쇄구균 신속항원검사의 유용성을 조사하였다. 방 법 : 2004년 9월부터 2005년 8월까지 한림대학교 강동성심병원 소아과를 방문하여 급성 인두염으로 진단받은 70명을 대상으로 하였다. 편도 삼출, 발열, 피부 발진 등의 임상적 특징과 내원 전 항생제 투여 여부를 조사하고, CBC, ASO, CRP 등의 혈액 검사와 인두에서 연쇄구균 신속항원검사와 배양검사를 시행하였다. 결 과 : 1) 인두배양검사에서 A군 연쇄구균이 분리된 양성례는 14례(20.0%)이었으며, 연쇄구균 신속항원검사는 22례(31.4%)에서 양성이었다. 인두배양검사에 대한 신속항원검사의 민감도는 78.6 %, 특이도는 80.4%이었다. 양성예측치는 50.0%, 음성예측치는 93.8%이었다. 2) 발열 유무에 따라 인두배양검사와 신속항원검사에는 차이가 없었다. 3) 편도삼출의 여부에 따라 인두배양검사 양성률에 차이가 없었으며, 신속항원검사는 오히려 편도삼출이 없는 경우에서 양성이 더 많았다. 4) 성홍열양 발진이 있는 경우에 인두배양검사와 신속항원검사에서 양성이 많았다. 5) 인두배양검사 양성은 항생제를 이전에 투여한 경우에 35례 중 3례(8.57%) 이전에 항생제를 투여하지 않은 경우의 35례 중 11례(31.43%)로 낮아 항생제 사용이 인두배양검사에 영향을 주었다. 이전에 인두배양검사는 양서d음성이어도 신속항원검사는 양성인 경향이 있었다. 6) 인두배양검사와 신속항원검사에 따라 혈액의 백혈구 수, ASO, CRP 값은 각각 통계학적인 차이가 없었다. 결 론 : 소아 급성 인두염에서 신속항원검사는 연쇄구균 감염의 진단에 유용한 도구로서 항생제의 조기 투여 여부를 결정하는 데 도움이 되리라 사료된다. Purpose : The purpose of this study was to evaluate the utility of rapid antigen detection test for group A streptococci in children with acute pharyngitis. Methods : Seventy children with acute pharyngitis enrolled the study, who visited the Department of Pediatrics, Kangdong Sacred Heart Hospital, Hallym Medical Center from September 2004 to August 2005. The presence of clinical features such as tonsillar exudates, fever, scarletiniform rash and previous antibiotic use were evaluated and throat swab culture, rapid antigen detection test for group A Streptococci, CBC, ASO, and CRP were performed. Results : Among 70 children with acute pharyngitis, the prevalence of group A beta-hemolytic Streptococcus by throat swab culture and rapid antigen detection test was 14(20.0 %) and 22(31.4%), respectively. The sensitivity of the rapid antigen detection test was 78.6 %, specificity was 80.4%, the positive predictive value was 50.0% and the negative predictive value was 93.8%. The positivity of the throat swab culture and rapid antigen detection test showed no signigicant differences according to the presence of the clinical symptoms except scarletiniform rash. Laboratory data such as CBC, ASO or CRP proved not valuable to predict the positivity of the throat swab culture and/or rapid antigen detection test for group A Streptococcus. There was a tendency of negative throat swab culture and positive rapid antigen detection test in cases of previous antibiotic use. Conclusion : The rapid antigen detection test can be a useful diagnostic tool for early detection of the group A Streptococcus in children with acute pharyngitis and can be helpful for the clinician to reduce the inappropriate use of antibiotics. [Pediatr Allergy Respir Dis (Korea) 2006;16:57-65]

항공간호업무 평가도구 개발

정지아 ( Ji A Jung ),김춘숙 ( Chun Suk Kim ),한선옥 ( Sun Ok Han ),박정현 ( Jung Hyun Park ) 국군간호사관학교 군진간호연구소 2014 군진간호연구 Vol.32 No.2

Purpose: The purpose of the research was to develop an instrument that could be used in measuring and evaluating the performance of aeromedical evacuation for the flight nurse. Method: Based on literature regarding aeromedical evacuation roles, we developed an instrument evaluating aeromedical evacuation performance for flight nurses. Researchers made a temporary tool and verified both content validity and face validity. Also the reliability and construct validity were verified. Result: The reliability of the instrument was measured by internal consistency. Cronbachs`` α was .99. The method of verification of construct validity was used to known group technique because of small number of Flight nurse. Conclusion: This performance evaluation instrument of aeromedical evacuation for flight nurses was verified by both the reliability and construct validity. In the future, this instrument will be used for evaluation and verification of aero medical evacuation practice by the flight-nurse.

Serratia marcescens 검출을 위한 PCR 기법 개발 및 돼지정액 유래균주에 대한 항생제 감수성 양상

정지아(Ji-A Jung),김애란(Aeran Kim),서병주(Byoung Joo Seo),정석찬(Suk Chan Jung),김인철(In Cheul Kim),정기화(Ki Hwa Chung),정병열(Byeong Yeal Jung) 한국생명과학회 2013 생명과학회지 Vol.23 No.9

돼지 원정액의 채취나 희석정액의 제조과정 중 세균오염이 많이 발생하는데, 이는 정자활력의 감소뿐 아니라 모돈의 수태율 저하 등을 유발한다. 특히 Serratia marcescens는 환경에 널리 존재하며 비위생적으로 제조된 정액에 많이 분리되고 있다. 본 연구에서는 S. marcescens의 신속한 동정을 위하여 PCR 기법을 개발하였으며, 국내 돼지정 액 유래 S. marcescens을 이용하여 최소생육억제농도(MIC) 등을 조사하고 유효 항생제를 선발하고자 하였다. 개발 PCR 기법은 S. marcescens에서만 306 bp의 특이 유전자 증폭산물을 형성하였으며, 기타 돼지정액에서 분리 보고된 균주나 Serratia 속균에서는 유전자 증폭 산물이 형성되지 않아 특이성이 인정되었다. PCR 기법의 민감도는 S. marcescens에서 추출된 DNA 50 pg/μl까지 검출이 가능하였다. 디스크확산법에 의한 국내 돼지정액 유래 S. marcescens의 항생제 감수성을 조사한 결과, gentamicin, ceftiofur, neomycin 등에서 80% 이상의 높은 감수성을 보였 다. 한편 ceftiofur, enrofloxacin, gentamicin, neomycin의 MIC90는 각각 8, 8, 8, 16 μg/ml로 나타났다. 따라서 개발된 PCR 기법은 S. marcescens를 동정하는 유용한 방법이며, gentamicin 등 선발된 항생제는 S. marcescens에 의한 정액 내 세균오염을 관리하기 위한 희석제용 항생제로 추천된다. During the collection of boar semen, bacterial contamination usually occurs. The contamination has deleterious effects both on semen quality and on sow fertility. The majority of contaminants are gram-negative bacteria, especially Serratia marcescens. In this study, we developed a PCR assay for the identification of S. marcescens targeting the luxS gene (GenBank no. EF164926). S. marcescens yielded a specific 306 bp PCR product. However, no amplification was observed in the other strains tested. The detection limit of PCR was 50 pg/μl of template DNA of S. marcescens. The antimicrobial susceptibility patterns of S. marcescens isolated from boar semen were tested using the disk diffusion method. Gentamicin, ceftiofur, florfenicol, and neomycin showed high sensitivity in this test. The minimum inhibitory concentration (MIC) was also determined by the broth microdilution method. The MIC90 values of ceftiofur, enrofloxacin, gentamicin, and neomycin were 8, 8, 8, and 16 μg/ml, respectively. These results indicate that PCR amplification of the luxS gene is a reliable and effective method for the identification of S. marcescens and that ceftiofur, enrofloxacin, gentamicin, and neomycin are effective semen extenders for controlling S. marcescens.

풍력 발전 예보 정확도 향상을 위한 국지 기상장 수치모의 개선 방안 연구

정지아(Ji-A Jung),이화운(Hwa-Woon Lee),전원배(Won-Bae Jeon),김동혁(Dong-Hyeok Kim),김현구(Hyun-Goo Kim),강용혁(Young-Heack Kang) 한국대기환경학회 2015 한국대기환경학회지 Vol.31 No.1

심와부 복통으로 내원한 5세 소아에서의 위 선근종 1례

허미영,정지아,최금자,성순희,서정완,Heo, Mi-Young,Jung, Ji-A,Choi, Kum-Ja,Sung, Sun-Hee,Seo, Jeong-Wan 대한소아소화기영양학회 2001 Pediatric gastroenterology, hepatology & nutrition Vol.4 No.1

심한 심와부 동통과 구토를 주소로 내원한 5세여아에서 상부 위장관 내시경으로 위유문동에서 중심요와를 보이는 비정상적인 주름을 관찰하였다. 복통이 계속되어 이소성췌장으로 생각하고 위부분절제술을 시행하여 치료한 위 선근종 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Adenomyomas of the stomach are rare tumors characterised by duct/gland-like structures embedded within a smooth muscle stroma. A 5-year-old female patient was admitted to the department of Pediatrics, Ewha Womans University MokDong Hospital with the history of severe epigastric pain and vomiting for 1 day. Esophagogastroduodenoscopy showed bridging fold with central dimpling on posterior wall of prepyloric antrum. Endoscopic biopsy was nondiagnostic. The patient complained epigastric pain continuously and underwent wedge resection. Pathologic examination showed an adenomyoma of the prepyloric antrum. After wedge resection, the patient did not complain epigastric pain during the postoperative follow-up. We report an unusual case of an adenomyoma of stomach.

대장 내시경으로 진단한 장결핵 1례

배상영,박선주,남승연,정지아,서정완,이선화,Bae, Sang-Young,Park, Sun-Joo,Nam, Seung-Yeon,Jung, Ji-A,Seo, Jeong-Wan,Lee, Sun-Wha 대한소아소화기영양학회 1999 Pediatric gastroenterology, hepatology & nutrition Vol.2 No.2

저자들은 심한 빈혈과 복통을 주소로 내원한 폐결핵을 동반하지 않은 9세 여아에서 시행한 대장내시경 소견과 생검 조직의 배양 검사로 진단하고 항결핵제 치료로 증상이 호전된 예를 경험하여 보고하는 바이다. Childhood intestinal tuberculosis is difficult to diagnose for its protean clinical manifestations, especially in cases without pulmonary involvement. Differential diagnosis with Crohn's disease, inflammatory bowel disease and other malignancy is also important. Surgery has often been required for pathologic confirmation or therapy. Colonoscopy may be performed safely under consciousness sedation in children for bacteriologic and histopathologic confirmation of the biopsy specimen in addition to gross appearance of the lesion. We have experienced a case of intestinal tuberculosis presenting with chronic abdominal pain, diarrhea, weight loss and anemia in a 9 year old girl who was diagnosed by a colonoscopic examination and culture of the biopsy specimen from the ascending colon. The patient was managed with antituberculous drugs and recovered uneventfully.

비.부비동염으로 내원한 소아환자들의 치료 유형별 만족도와 치료 평가

임영권,김현경,허광욱,정지아,이훈,윤철상,김호철,Lim, Young-Kwon,Kim, Hyun-Kyung,Hur, Kwang-Wook,Jung, Ji-A,Lee, Hun,Yun, Cheol-Sang,Kim, Ho-Cheol 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.3

Objectives The purpose of this study is to investigate the effects of simultaneous treatment with supplementary therapy and treatment interval for improving symptoms and satisfaction rate by treating child rhinitis or paranasal sinusitis patients. Methods 41 rhinitis or paranasal sinusitis patients who visited the clinic between April 2004 and April 2006 were involved for this study. The patients were classified into Group A(2 sessions per week, simultaneous supplementary therapy), Group B(1 session in 2 weeks, simultaneous supplementary therapy) and Group C(1 session in 2 weeks, no supplementary therapy). After the experiment, the improvement rates of symptoms and satisfaction rate were surveyed by the questionnaires. Supplementary therapies used in Group A and B were aroma spray, Saengbit-patch, cutaneous acupuncture, nebulizer and Chuna manipulation. Results From 41 of the rhinitis or paranasal sinusitis patients, the Group A(simultaneous treatment group, 2 sessions of intensive care per week with supplementary therapy) showed the remaining symptoms score of $12.1{\pm}10.0$ in average. This score was the lowest comparing with the average of $25.7{\pm}12.9$ in Group B(1 session in 2 weeks, simultaneous treatment group with supplementary therapy) and the average of $21.0{\pm}20.1$ in Group C(1 session in 2 weeks, no supplementary therapy). Nose symptoms and sleep scores were significantly lower in Group A(p<0.01). The satisfaction score was the highest in Group B that had the most remaining symptoms and it had no significant relationship with the symptom improvement. Additional analysis showed that among many factors such as treatment interval, treatment method, cost, accessibility of the clinic, family history and satisfaction with the doctor; the satisfaction with their doctor was the most closely related to the satisfaction rate of the patients. Conclusions In this study with 41 of rhinitis or paranasal sinusitis patients, the 2 sessions of intensive care group showed the best results in improving symptoms. Supplementary treatments were also used simultaneously; aroma spray, cutaneous acupuncture, Saengbit-patch, Chuna manipulation and nebulizer. The questionnaires showed that the satisfaction rate of the patients(care taker) was not related to the symptom improvement. Additional analysis of the factors that influence the satisfaction rate showed that it was more closely related to the satisfaction with their doctors.

국가 종축우에 대한 요네병 항체 양성률 조사

Ha-Young Kim(김하영),Jae-Won Byun(변재원),Albert Byungyun Jeon(전병윤),Bum Soo Park(박범수),Ji-A Jung(정지아),Mihak Park(박미학),Yeon-Su Lim(임연수),Byeong Yeal Jung(정병열) 한국생명과학회 2012 생명과학회지 Vol.22 No.6

요네병은 Mycobacterium avium subspecies paratuberculosis에 의해 발생하며 반추동물에서 만성 쇠약을 일으켜 전세계적으로 양축산업에 커다란 경제적 손실을 일으키는 질병이다. 본 연구에서는 2008년부터 2009년까지 2년동안 의뢰된 총 3,927건의 국가 종축우의 혈청에 대한 요네병의 항체 양성률에 대해 조사한 결과 21두(0.5%)에서 항체 양성을 나타내었으며 이는 국내 일반 농가의 양성률(1.2-16.4%) 보다 현저히 낮은 수준이었다. 또한 연령별 양성률은 2세 미만에서 0.2% (3두), 2-3세에서 0.4% (2두), 3-4세에서 0.7% (3두), 4세 초과에서 0.9% (13두)로 전연령에서 양성이 나타났으나 연령이 높아짐에 따라 양성률이 증가하는 경향을 보였다. 품종 별로는 젖소는 모두 음성이었으며 한우에서만 양성률 0.6%를 나타내었다. Paratuberculosis (Johne’s disease), a chronic wasting disease caused by Mycobacterium avium subspecies paratuberculosis (MAP), is a major cause of economic loss in the cattle industry. In Korea, national monitoring of breeding stock for MAP has been implemented. In this study, we report the results of serological testing to determine the prevalence of MAP in breeding stock of Korean native and dairy cattle during 2008 and 2009. A total of 3,927 serum samples were submitted (3,692 Korean native cattle and 235 dairy cattle) to Animal Disease Diagnostic Division, Animal, Plant and Fisheries Quarantine and Inspection Agency. The samples were classified into four different age groups for MAP; group 1 (≤2 year, n = 1,509), group 2 (>2 years to≤3 years, n = 486), group 3 (>3 years to ≤4 years, n = 441), and group 4 (>4 years, n = 1,491). Overall seroprevalence of MAP in this study was 0.5% (21/3,927), which was much lower than that of conventional cattle (1.2-16.4%) in Korea. Also, the seroprevalence was determined by age groups: three of group 1 (0.2%), two of group 2 (0.4%), three of group 3 (0.7%), and 13 of group 4 (0.9%) were seropositive for MAP, respectively. Although seropositive samples were found in all age groups, the seroprevalence tended to increase with age. Our study showed that the seroprevalence of MAP in pure-bred breeding dairy cattle (0%) was lower than that in pure-bred breeding Korean native cattle (0.6%).

한약의 아토피 피부염 치료에 대한 예비 임상 연구

임영권,정지아,윤철상,허광욱,이훈,김호철,김영란,조윤희,Lim, Young-Kwon,Jung, Ji-A,Yun, Cheol-Sang,Hur, Kwang-Wook,Lee, Hun,Kim, Ho-Chyul,Kim, Young-Ran,Cho, Yun-Hi 대한한방소아과학회 2006 대한한방소아과학회지 Vol.20 No.3

Objectives : The purpose of this study is to determine clinical efficacy of herbal medicine by evaluating SCORAD Index and total level of ceramaides in the skin of 13 children with atopic dermatitis Methods : Subjects are divided into two groups : Group 1(non-differentiation children treated with Saenghyeoryunbueom) and group 2(differentiation children treated with either pyungwisan, onchungum or gamitongsungsan). We determine SCORAD Index and total level of ceramaides in the skin of 13 children with atopic dermatitis before and after taking each of herbal treatment for 12 weeks. Results : After herbal prescription for 12 weeks, A SCORAD index of both group 1 and group 2 was decreased. However, the total level of ceramides in group 1 and group 2 was not altered after 12 weeks. When the correlation between the alteration of SCORAD index and ceramides levels was determined, the SCORAD index in group 1 was inversely correlated with the total level of ceramides(r=-0.994, p=0.006) In contrast, the alteration of SCORAD index in group 2 was not correlated with ceramide levels. Conclusions : The clinical efficacy of Saenghyeoryunbueom for non-differentiation children with atopic dermatitis is paralleled with the increased level of ceramides in skin. The clinical efficacy of pyungwisan, onchungum or gamitongsungsan for differentiation children with atopic dermatitis is not correlated with ceramide level in skin.

Agrobacterium-mediated transformation of ‘GeumNodaji-Euncheon’ oriental melon

Jun Young Choi(최준영),Ji A Jung(정지아),Nam In Hyung(형남인) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.5