全蝎 抽出物이 血栓症, 全腦虛血 및 腦細胞毒性에 미치는 影響

백명현,황영근,정지천,강정준,김성훈 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

본 실험은 전갈 추출물이 어혈병태 모델과 KCN으로 유발된 전뇌허혈, 뇌세포독성 등에 미치는 영향을 관찰하였다. 전갈은 Dextran에 의해 유도된 혈전으로 감소된 혈소판수, fibrinogen량, hemocrit치를 유의성 있게 증가시키고. 증가된 prothrombin time을 유의성 있게 단축시켰다. 전갈은 thrombin과 ADP에 의해 유도된 혈소판 응집을 억제하였으나 collagen에 의해 유도된 혈소판 응집에는 저해효과를 나타내지 않았다. 또한 collagen과 epinephrine에 의해 유도된 pulmonary embolism에 대하여 보호 효과를 나타내었다. 전갈은 KCN에 의한 전뇌허혈 유발 실험에서 혼수시간을 유의성 있게 단축시켰고, Amyloid β protein(25-35)에 의해 유도된 PC12 세포의 독성에 대하여 보호효과를 나타내었다. This following is effect of Buthus martensi Karsch (BMK) extract on dextran-thrombus model, KCN-induced coma, cytotoxicity of brain etc. BMK extract significantly increased number of platelet and fibrogen and significantly shortened the prothrombin time as compared with control group treated with dextran. BMK extract didn't affect the changes of hematocrit as compared with control group treated with dextran. BMK extract induced a significant inhibition of human platelet aggregation induced by thrombin and ADP but did not affect human platelet aggregation induced by collagen. BMK extract showed a protective effect on pulmonary thrombosis induced by collagen and epinephrine. BMK extract prolonged the duration of KCN-induced coma and showed a protective effect on cytotoxicity of PC12 cells induced by amyloid β protein(25-35) in a dose dependent manner. These results suggested that BMK extract might be usefully applied for prevention and treatement of thrombosis and brain damage.

골수염으로 발현된 재발성 콕시디오이데스 진균증 1예

백지현,박은영,정윤숙,홍재원,채윤태,진성준,최희경,신소연,한상훈,진범식,김창오,최준용,송영구,조남훈,김준명 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Coccidioidomycosis is a fungal infection that results from inhaling the airborne arthroconidia of the Coccidioides species. It is an endemic disease in the southwest part of North America and rarely diagnosed in Korea. As tourism to endemic areas and the number of immunocompromised patients have been increasing, the incidence of this infection has increased in non-endemic areas. Treatment is usually successful with antifungal agents; however, recurrence is common. It is difficult to decide when to discontinue the antifungal treatment especially in non-endemic areas where doctors are not familiar with the disease. We report a case of recurrent coccidioidomycosis manifesting as osteomyelitis after the treatment of the patient for disseminated coccidioidal infection. The complement fixation test was a useful tool for the assessment of patient response and to evaluate suspected recurrence.

Methylphenidate 투약에 따른 주의력결핍 과잉운동장애 아동의 정량화 뇌파의 변화

이병대,정성훈,이만기,장수민,백지령,임효덕 대한생물치료정신의학회 2002 생물치료정신의학 Vol.8 No.1

CC Chemokine CKβ8의 발현 조절 물질 탐색 및 저해활성 물질 분석

김탁용,이태훈,백남인,정대균,김지영 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

CKβ8은 CC 케모카인의 부류에 속하는 사이토카인의 일종으로, 단핵구, 호염구, 호산구, T 림프구, 자연살해세포, 수지상세포 등의 화학주성물질로 작용하는 것으로 알려져 있다. CKβ8은 염증반응 및 혈관신생 등과 같은 여러 가지 생체 반응에 역할을 하는 것으로 알려져 있다. 본 논문에서는 사람 CKβ8 유전자의 전사저해 물질을 선별하기 위하여 CKβ8/Luc 유전자를 발현하는 세포주를 확립하였다. CKβ8 유전자의 전사시작점에서 -299 부터 +10 프로모터 부위를 PCR에 의해 얻은 다음 포유세포 발현 플라스미드에 클로닝하였다. CKβ8/Lvc 유전자는 U937 세포내에서 PMA에 의해 10배 이상 발현이 증가한다. CKβ8/Luc 발현 U937 세포주는 식물 추출물에서 전사 저해제를 탐색하는데 이용되었다. 46 개의 식물 추출물 중에서 6개가 90% 이상 CKβ8/Luc의 전사를 저해하였다. 이들 물질에 대해 세포 독성 실험을 수행한 결과 그 중 한 개만이 세포 독성이 거의 없는 것으로 나타났다. 이 추출물은 붉나무에서 얻은 추출물이며 이 식물 추출물은 염증관련 질환에 대한 가능성이 있는 치료제로 이용될 수도 있을 것으로 추측된다. Ckβ8 belongs to a family of CC chemokines, which is known to act as a chemoattractant for monocytes, basophils, eosinophils, T lymphocytes, NK cells, and dendritic cells. CKβ8 is known to play roles in various biological processes such as inflammation and angiogenesis. In the present study, we developed U937 cell line expressing CKβ8/Luciferase fusion gene for screening of transcriptional inhibitors for human CKβ8 gene. Genomic sequences that contain CKβ8 promoter region from 299 to+10 nucleotide from the transcription start site was amplified by PCR and cloned into mammalian episomal plasmid. The CKβ8/Luc fusion plasmid was introduced into human promonocytic U937 cells and selected for stable U937 cells expressing the fused reporter gene. The expression of the fusion gene was induced by more than 10 folds upon treatment of phorbol myristate acetate. The stable U937 transfectant cells were used for searching transcriptional inhibitors for human CKβ8 gene from plant extracts. Among 46 plant extracts tested, six extracts exerted more than 90% inhibition of PMA-induced CKβ8 transcription. Cytotoxic tests demonstrated that one of extracts, which was extracted from Rhus chinensis Mill, had little cytotoxic effect. The extract may be used for developing a potential therapeutic agent for inflammatory diseases.

음성 인식 헬스케어 시스템

신진우,조우승,조성빈,김재훈,정지은,사예지,백경동,박동규 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

As modern people's interest in health grows, wearable healthcare-related technologies that can collect biometric information and receive health-related services regardless of time and place are developing. This paper proposes the healthcare system based on voice recognition function. Unlike conventional wearable healthcare systems, the proposed system provides real-time telemedicine and diagnosis and the direct connection with doctors is possible through it. And almost all functions of the application in it are operated with voice commands to lower user access barriers. Because it deals with personal information including medical information, encryption algorithms have been applied between all data movements. Finally, the healthcare system proposed in this paper is expected to increase convenience in health care and reduce the medical gap.

노인 인구에서 신경인지기능저하와 혈청 지질농도와의 상관성

유영선,최석주,정성수,김지은,윤성욱,전동욱,백준형,박성우,이정구,추일한,김영훈 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.6

Objectives : In this study, the authors evaluated the correlation between eurocognitive impairments and serum lipids levels among Korean elderly over the age of 65. Methods : A total of 609 elderly individuals participated in this study. Screening for cognitive impairments were carried out using the Mini-Mental Status Examination-Korean version (MMSE-KC). There were 197 subjects above 1.5SD Of MMSE- KC and they were evaluated using the Korean versions of the Consortium to Establish a Registry for Alzheimer's Disease (AD) (CERAD-KC) and Geriatric Depression Scale (GDS-K). Results : There was a correlation between old age/low levels of education and AD (p<0.05, P<0.01). There was an inverse correlation between the serum level of total cholesterol and the word list recognition test scores. The serum levels of LDL cholesterol were inversely correlated with the scores on the constructional praxis test, word list recall test and word list recognition test. Inverse correlations between the serum level of triglyceride and scores on the word list recall test and word list recognition test were also observed. Conclusion : There were inverse correlations between the serum levels of lipids and language/memory function in subjects with AD.

Development and evaluation of a triplex real-time quantitative reverse transcription-polymerase chain reaction for rapid and differential detection of three feline respiratory viral pathogens

( Ji-su Baek ),( Jong-min Kim ),( Hye-ryung Kim ),( Ji-hoon Park ),( Yeun-kyung Shin ),( Hae-eun Kang ),( Jung-hoon Kwon ),( Won-jae Lee ),( Min Jang ),( Sang-kwon Lee ),( Ho-seong Cho ),( Yeonsu Oh ) 한국동물위생학회 2023 韓國家畜衛生學會誌 Vol.46 No.4

In this study, a new triplex real-time quantitative reverse transcription polymerase chain reaction (tqRT-PCR) assay was developed for the rapid and differential detection of three feline viral pathogens including feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), and influenza A virus (IAV) in a single reaction. The assay specifically amplified three targeted viral genes with a detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with intra- and inter-assay coefficients of variation of less than 1%. Based on the diagnostic results of the assay using 120 clinical samples obtained from cats with feline respiratory disease complex (FRDC)-suspected signs, the prevalence of FCV, FHV-1, or IAV was 43.3%, 22.5%, or 0%, respectively, indicating that the diagnostic sensitivity was comparable or superior to those of previously reported monoplex qRT-PCR/qPCR assays. The dual infection rate for FCV and FHV-1 was 8.3%. These results indicate that FCV and FHV-1 are widespread and that co-infection with FCV and FHV-1 frequently occur in the Korean cat population. The developed tqRT-PCR assay will serve as a promising tool for etiological and epidemiological studies of these three bacterial pathogens, and the prevalence data for three feline viruses obtained in this study will contribute to expanding knowledge about the epidemiology of FRDC in the current Korean cat population.

Development and utility evaluation of new Multi-Leaf Collimator for Diagnostic X-ray Equipment

Ji, Hoon,Han, Su Chul,Baek, Jong Hyeun,Lee, Dong Hoon,Park, Seungwoo The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.2

The diagnostic multi-leaf collimator preventing unnecessary dose from entering into patients during the diagnostic examination was made in this study. The movement of the entire 50 leaves was embodied with the group of 25 ones thereof configured in a pair facing each other on the left and right of the median line. Dimensions of the length, width, and height of each shielding leaf were $5{\times}0.5{\times}0.5cm^3$ resulting in the maximum boost field of $10{\times}10cm^2$. The material of multi-leaf collimator had the excellence on the machinability with the use of the SKD-11 alloy tool steel having the high wear resistance against frequent movement, and it was devised to control both-side's shielding leaves by moving 2 motors unlike existing remedial multi-leaf collimator that use as many motors as the number of 50 shielding leaves. Thereafter, the transmission dose of leaves, cross-leaf leakage dose, and inter-leaf leakage dose were measured by the developed multi-leaf collimator attached to X-ray equipment. An ionization chamber was used to detect doses there from, and the comparative analysis was carried out by means of the radiographic film that was easy to detect the dose leakage in between each leaf. Results obtained from the test conducted in comparative analysis yielded approximately 98%, 96%, and 94% of shielding efficiency realized at each level of energy of 80kV, 100kV, and 120kV it was confirmed there was no dose leakage resulted from the varied level of irradiation energy. Thus the multi-leaf collimator to be developed based on this study is thought that it could fully reduce the unnecessary dose to patients in the diagnostic test and the shielding efficiency thereof is expected to be increasing if it is made in a miniaturized form with a way of increasing the thickness of each leaf later for an extended application to general diagnostic purposes.

Development and utility evaluation of new Multi-Leaf Collimator for Diagnostic X-ray Equipment

Hoon Ji,Su Chul Han,Jong Hyeun Baek,Dong Hoon Lee,Seungwoo Park 대한전기학회 2018 Journal of Electrical Engineering & Technology Vol.13 No.2

The diagnostic multi-leaf collimator preventing unnecessary dose from entering into patients during the diagnostic examination was made in this study. The movement of the entire 50 leaves was embodied with the group of 25 ones thereof configured in a pair facing each other on the left and right of the median line. Dimensions of the length, width, and height of each shielding leaf were 5×0.5×0.5㎤ resulting in the maximum boost field of 10×10cm². The material of multi-leaf collimator had the excellence on the machinability with the use of the SKD-11 alloy tool steel having the high wear resistance against frequent movement, and it was devised to control both-side’s shielding leaves by moving 2 motors unlike existing remedial multi-leaf collimator that use as many motors as the number of 50 shielding leaves. Thereafter, the transmission dose of leaves, cross-leaf leakage dose, and inter-leaf leakage dose were measured by the developed multi-leaf collimator attached to X-ray equipment. An ionization chamber was used to detect doses there from, and the comparative analysis was carried out by means of the radiographic film that was easy to detect the dose leakage in between each leaf. Results obtained from the test conducted in comparative analysis yielded approximately 98%, 96%, and 94% of shielding efficiency realized at each level of energy of 80kV, 100kV, and 120kV it was confirmed there was no dose leakage resulted from the varied level of irradiation energy. Thus the multi-leaf collimator to be developed based on this study is thought that it could fully reduce the unnecessary dose to patients in the diagnostic test and the shielding efficiency thereof is expected to be increasing if it is made in a miniaturized form with a way of increasing the thickness of each leaf later for an extended application to general diagnostic purposes.

Case Reports : Development of Bullous Acrodermatitis Enteropathica during the Course of Chemotherapy for Acute Lymphocytic Leukemia

( Ji Hoon Chun ),( Ji Hye Baek ),( Nak Gyun Chung ),( Jung Eun Kim ),( Baik Kee Cho ),( Hyun Jeong Park ) 대한피부과학회 2011 Annals of Dermatology Vol.23 No.3s

Acrodermatitis enteropathica (AE) is an uncommon autosomal recessive genetic disorder of zinc malabsorption. The acquired form may be associated with inadequate intake, impaired absorption, and increased excretion of zinc. Those afflicted present with diarrhea, stomatitis, psychiatric symptoms, non-scarring alopecia, and nail dystrophy accompanied by erythematous which appears as scaly patches with erosion vesicles and pustules mostly affecting the extremities, perineal, and periorificial areas. Due to the variable findings of most case reports, the clinical and histopathological features of AE are often regarded as non-specific. We report an unusual case of bullous AE secondary to total parenteral nutrition for the treatment of acute pancreatitis occurring in a six-year-old male with acute lymphocytic leukemia who underwent chemotherapy. He presented with periorificial, reddish, eroded bullae with multiple vesicles and blisters on his fingers, toes, and buttock, showing necrotic keratinocytes with multiple intraepidermal vesicles and perivascular infiltration with predominant lymphocytes and few neutrophils within the dermis. To the best of our knowledge, this is the first case report of bullous AE in the Korean dermatologic literature. (Ann Dermatol 23(S3) S326~ S328, 2011)