SD Rats를 이용 1-hexene의 흡입독성 연구

김현영,임철홍,정용현,이권섭,이성배,이준연,한정희,전윤석,이용묵 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4

The purpose of this study was to investigate the acute (4 hours) and repeated-dose (6 hours a day, 5 days a week, 4 weeks) toxic effects of I -hexene on Sprague- Dawley (SD) rats which were treated by inhalation. The results were as follows; I. The median lethal concentration (LC_(50)) was estimated 52,694 ppm (confidence limit 95%; 49,494~55,447 ppm) in acute inhalation. Abnormal clinical signs related to the l-Hexene were not observed with the acute inhalation dose. Gross findings of necropsy revealed on evidence of specific toxicity related to the 1-hexene, II. By repeated inhalation exposure the body weight of male were more or less reduced by the dose of 2,500 ppm and 5,000 ppm compared with control group. However there were no significant variation hematology and blood biochemistry for the exposed rats compared with the control rats. Abnormal clinical signs and gross findings of necropsy related to the 1-hexene were not shown. In conclusion when we exposed I-hexene to SD rats for 4 weeks, 5 days per week, 6 hours per day, the Lowest observed effect level (LOEL) was over 2,500 ppm and Non observed effect level (NOEL) was below 500 ppm.

過失相計에 관한 判例의 動向

이준현(Lee Jun?Hyeon) 숭실대학교 법학연구소 2011 法學論叢 Vol.25 No.-

채무불이행이나 불법행위로 인한 손해배상책임영역에서 손해의 공평, 타당한 분담을 실현하기 위해서는 과실상계제도가 있어야 한다는 점은 누구나 다 공감하는 부분이다. 그러나 우리 민법은 이러한 과실상계제도에 관하여 채무불이행으로 인한 손해배상에서 한 개의 조문을 두면서, 매우 간결하게 채무불이행이나 불법행위로 인한 손해배상책임의 영역에서 채권자(피해자)에게 과실이 있는 경우 법원은 손해배상의 책임 및 그 금액을 산정함에 있어 그 채권자(피해자)의 과실을 참작하여야 한다고 규정하고 있다. 즉, 과실상계에서 가장 중요한 개념인 채권자(피해자)의 과실(손해의 기여도)과 그 판단능력에 대해서는 전혀 규정을 두고 있지 않으며, 또한 이를 참작한다고 할 때 그 비율이나 정도, 과실상계의 구체적 적용범위 등에 관하여는 그 규정을 두고 있지 않다. 이러한 우리 민법의 입법태도는 구체적 사건에 임하여 법관이 전문가적 소견에 따라 당해사건과 관련된 제반사정을 종합적으로 고려하여 가장 공평하고 타당한 손해배상책임범위를 정하게 한다는 점에서는 일단 부당하다고 할 수는 없는 것이나, 과실상계의 구체적 내용에 대하여 민법이 직접 규정하지 않고 판례에만 맡김으로써 과실상계의 운용과정에서 법관의 자의적 내지 독단적 판단이 개입할 우려가 있는 것도 부인하지 못할것이다. 앞으로 과실상계에 관한 구체적 내용을 대법원 판례에만 의지할 것이 아니라, 민법개정시에 적극적으로 반영하여 입법화할 필요가 있다고 하겠다. 여하튼 과실상계제도의 취지를 고려하여 대법원은 과실상계에 관한 요건 및 효과, 적용범위 등에서 다양한 입장을 나타내고 있다. 채무불이행이나 불법행위로 인하여 발생된 손해를 공평, 타당하게 분담하고 과실상계제도의 운용과정에서 법적안정성이나 예측가능성을 지켜가기 위해서는 과실상계에 관한 대법원 판례의 전면적인 검토가 이루어져야 할 것이다. 본 논문에서는 현재까지의 과실상계와 관련된 대법원 판례의 동향을 살펴보고 그 타당성에 대하여 상세히 검토해 보고자 한다. 그럼으로써 손해의 공평, 타당한 분담을 위한 과실상계제도가 정당하고 예측가능하게 운용되는 데 기여할 것으로 기대한다. Everyone sympathizes that there should be a comparative negligence system, in order to actualize impartial and valid contibution of the damage due to default or tort in the filed of damage liability. But Korean civil law in compensation for damage due to default on comparative negligence has one article and regulates very briefly that a court should consider negligence of creditor (victim) in the filed of damage liability due to default or tort in case of a negligence of creditor (victim) in measuring damage liability and its amount. That is to say, there is no regulation in Korean civil law the negligence (related degree of damage) of a creditor (a victim) and the judgement capability the most important concept in the comparative negligence. Also there is no regulation in considering these, the rate and the degree, specific applicable range. This attitude of legislation in Korean civil law - in case of the judge with his own professional opinions considers the general circumstance in relation to the concerned cases synthetically and decides the most impartial and valid range of damage liability in specific cases - can not be stated unreasonable. But on the specific contents on the comparative negligence due to leave only precedent without direct regulating in civil law, in the operational process of the comparative negligence, apprehension to the arbitrary and dogmatic judgement of the judge intervenes can not be denied. In the future the specific contents on the comparative negligence will not depend on the precedent of Korean Supreme Court but it is necessary to legislate positively with a reflection of this content in the time of revision of the Korean civil law. Anyway in considering the intent of comparative negligence, Supreme Court of Korea shows various position in the requirement and effect, applicable range on comparative negligence. The damage occurred due to default and tort should apportion fair and validly in order to keep the legal stability and the predictable possibility in the operational process of the comparative negligence, overall examination on the precedent of the Supreme Court of Korea on the comparative negligence should be carried out. In this paper the author tries to search the trend of precedent of the Supreme Court in Korea on comparative negligence up to the present and examine on its validity in detail. By this study the comparative negligence system for impartial and valid contirbution of the demage, expects to contribute to operate fairly and predictably.

Morgagni 탈장 : 1례 보고

오연희,이창욱,김승현,이성우,정병욱,이준희,박동일,권영무,신현종 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

Morgagni 탈장은 횡격막의 전내측 부위의 발달이상으로 열공을 통하여 장관이나 대망과 같은 복강내 구조물이 흉곽내로 빠져나가는 드문 질환으로 알려져 있다. 저자들은 호흡곤란을 주소로 내원하여 방사선학적 검사와 수술로 확진된 1례를 보고한다. Morgagni hernia is improperly located abdominal viscera into the thoracic cavity through the foramen of Morgagni which is a congenital defect at the anterornedial portion of diaphragm. We report a case of Morgagni hernia diagnosed by radiologic examination and operation.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

사료용 교잡종 옥수수 연구 : Ⅲ. CNU와 SK계통을 이용한 교잡종 옥수수의 건물중 및 종실 수량

이희봉,김동욱,김준표,김용일,정재영,최현구,문현귀,이충열 충남대학교 농업과학연구소 2003 농업과학연구 Vol.30 No.2

공시 교잡종에 대한 각 지역에서 선발된 우수 교잡종의 간장 및 착수고는 대조구와 비슷한 경향이었으며, 생체 및 건물수량은 분얼수 및 간장과 관련이 큰 것으로 나타났으나 재식 밀도와 기상상태와도 밀접한 관계로 나타났다. 따라서 생체중은 대전지역에서 재배된 교잡종 H67이 대조구인 광안옥에 비해 53.2%로 높았고, 수원에서 역시 41.8%으로 높았으며, 종실수량은 H15과 H67에서 대조구보다 11.7%와 9.7%로 각각 높게 나타났다. 대전에서 분얼성인 H42와 무얼성인 H65에서 대조구보다 9.1%, 5.2%증가하였고, 수원에서는 분얼성이 H42와 무얼성인 H65에서 대조구보다 9.1%, 8.5% 증가하여 교잡종간, 지역간에 큰 차이를 보였다. 한편 후기 녹체성은 무얼형보다 분얼형에서 낮은 경향을 보였는데 SK계통과 교배된 조합에서 우수한 편으로 나타나 우수 교잡종 육성시 중요 고려사항으로 지적되었다. In order to develop the crude forage maize hybrids with high dry matter and grain yield, several hybrids evaluated as superior hybrids at three regions in 2001. They were planted and surveyed again for the second selection in 2002. The selected hybrids showed large difference in some major characteristics according to planting areas and dates(Suwon : April 25. Daejeon : May 15 and Milyang : May 25), but insect and disease injury were similar to check. Fresh and dry weight of the H67 hybrid at Suwon and Daejeon was the highest among hybrids including check, but low in Milyang due to raining after flowering. Grain yield was higher than check in H15 at Suwon H45 and H37 at Daejeon and H67 at Milyang.

백서 두개관세포에서 Ipriflavone이 골조직 개조에 미치는 영향

이용승,김영준,이기헌,황현식 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.4

본 연구는 ipriflavone (isopropoxyisoflavone)의 투여가 백서 두개관세포의 증식과 골조직 개조에 미치는 영향을 알아보고자 시도되었다. 태령 20-21일째의 백서 두개관세포를 분리 배양한 후, 10^(-9) M부터 10^(-5) M까지 농도의 ipriflavone을 투여하고 1일째와 3일째에 MTT 분석을 시행하여 흡광도를 평가한 결과, 모든 농도에서 백서 두개관세포의 증식을 보이지 않았다. 한편 골조직 개조에 미치는 영향을 알아보기 위하여 14일째에 alizarin red염색을 시행하여, 형성된 석회화 결절 면적을 측정하였을 때, 10^(-8) M, 10^(-7) M, 10^(-6) M 농도를 투여한 경우 석회화 결절 형성이 유의하게 증가하였다. 골아세포의 분화에 미치는 영향을 알아보기 위하여 ipriflavone을 투여하고 7일째와 14일째에 추출한 RNA를 역전사 중합효소 연쇄반응(RT-PCR)을 시켜 bone sialoprotein (BSP), type 1 collagen (COL I), osteocalcin (OCN) 유전자 발현을 관찰한 결과 BSP와 COL I 유전자는 배양 7일째 높은 발현을 보였고, OCN 유전자는 배양 14일째 높은 발현을 보였다. 이상의 연구결과 ipriflavone이 백서 두개관세포에서 석회화를 촉진시키고 골아세포의 분화에 관여하는 BSP, COL I 및 OCN 유전자 발현을 증가시켜 골조직의 개조를 빠르게 할 수 있음을 시사하였다. lpriflavone (isoprofoxyisoflavone), a synthetic derivative from soy isoflavone diazein, has been shown to inhibit bone resorption and perhaps stimulate bone formation. This study was performed to examine the effects of ipriflavone on the proliferation and bone remodeling in rat calvarial cells in vitro. The rat calvarial cells were isolated from fetus aged 20 to 21 days and cultured in BGJb media. The graded concentration of ipriflavone (10^(-9)-10^(-5) M) was administered into cultured cells. When the cell proliferation was estimated through the measurement of MTT assay, there was no increase in cellular proliferation of the rat calvarial cell at any ipriflavone concentration. The cellular activity was evaluated through the formation of mineralized nodules stained by alizarin red. The formation of mineralized nodules significantly increased at concentrations of 10^(-8) M, 10^(-7) M and 10^(-6) M ipriflavone. Reverse transcription-polymerase chain reaction analyses (RT-PCR) were done at 7 and 14 days after culture to detect the expression of Bone Sialoprotein (BSP), Type I collagen (COL I) and Osteocalcin (OCN). As a result, the expressions of BSP and COL I increased of the 7th day of culture and the expression of OCN increased on the 14th day of culture. These results indicate that ipriflavone facilitates the bone remodeling process by promoting rat calvarial cell differentiation and stimulating mineralization through increased expression of extracellular matrix genes, such as BSP, COL I and OCN.

월악산 잣나무 및 화백나무 형성층의 계절적 활동과 적산온도와의 관계

박준희, 강현정, 이해진, 서정욱 충북대학교 농업과학기술연구소 2022 農業科學硏究 Vol.38 No.2

The current study was conducted to investigate (1) the duration of cambial activity and (2) the effect of degree days on the initiation of cambial activity of the Pinus koraiensis (PK) and Chamaecy- paris picifera (CP) planted in Mt. Worak. In addition, the differences of the seasonal cambial activities according to the diameter were also investigated using C. picifera with a diameter of 30 cm (CPL) and 15 cm (CPS), which were planted in the same year. The cambium samples were weekly collected using a mini-borer (Ø 2 mm), so called Traphor, between April and October in 2017. The results obtained are as follow. The initiation of the cambial activity of CP (April 21-28) were slightly earlier than PK (April 21). The species with the longest cambial activity was CPS (147.2±12.1 days), followed by PK (141.6±9.8 days) and CPL (139±12.1 days). The degree days inducing the initiation of the cambial activ- ity for PK and CP were 180.5 and 180.5-242.8, respectively.

에어리프트 펌프를 이용한 정밀여과막 세척

이준호,김명호,고대현 韓國交通大學校 2022 한국교통대학교 논문집 Vol.57 No.-

The MBR method, also known as the membrane separation active sludge method, does not require a precipitation tank and has many advantages such as stable discharging water. The biggest disadvantages of the MBR method, which has many advantages, are maintenance problems due to reduced flux due to fouling, decreased processing efficiency, and membrane replacement. In this study, in order to reduce membrane pollution, membrane cleaning was performed using the washing principle of the airlift pump, and shear force according to the amount of air was analyzed using the Ansys Fluent computational fluid dynamics program. As a result of the analysis, the film recovery rate was 93.8% to 97.3% and the average was 95.8%, and the CFD analysis showed the highest shear force at about 5.425×10-2Pa at 10m3/m2/hr. It is believed that the MBR device using Airlift in this study can maximize the flux recovery rate by reducing the problem of membrane contamination.

제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산

이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.

고 탁도 건설 폐수 응집 특성 분석

이준호, 김명호, 고대현 韓國交通大學校 2020 한국교통대학교 논문집 Vol.55 No.-

In this study, we tried to find an efficient coagulation agent by comparing and analyzing the coagulation properties of each coagulation agent by conducting a Jar-test on various coagulation agents used for the efficient treatment of high turbidity construction wastewater. The Jar test was conducted with wastewater with a turbidity of 1,150 NTU and SS 1500 mg/L using yellow soil. PACS-2, PACSS, PACSM, PACS300, Chitosan, and Alum were tested in Jar-test and the turbidity, pH, SS, standing analysis, filtration time, etc. were analyzed to compare the cohesive characteristics of each floc. PACS-2 ranges from turbidity 36.9~124 NTU (average 82.24 NTU), pH 6.934~7.100 (average 7.025) and SS 20~70 mg/L (average 45.2 mg/L). PACSS appeared to range from turbidity 6.59~59.3 NTU (average 21.29 NTU), pH 5.166~7.010 (average 6.331), and SS 6~28 mg/L (average 13.6 mg/L). Also PACSM was found to range from turbidity 4.16~94 NTU (average 35.18 NTU), pH 5.742~6.908 (average 6.582), and SS 4~118 g/L (average 34 mg/L). PACS300 ranged from turbidity 5.22~85.7 NTU (average 31.07 NTU), pH 5.968~6.875 (average 6.603) and SS 4~66 mg/L (19.6 mg/L on average). Chitosan was found to be in the range of turbidity 7.3~50.1 NTU (19.58 NTU on average), pH 6.738~7.097 (6.900 on average), and SS 4~32 mg/L (13.6 mg/L on average). Finally, the Alum was found to range from turbidity 22~217 NTU (average 132.72 NTU), pH 6.878~7.113 (average 6.984), and SS 22~224 mg/L (average 115.2 mg/L). As a result of the experiment, Chitosan is considered to have a high coagulation characteristics as a coagulation agent of high turbidity construction wastewater due to low change in pH and excellent coagulation characteristics.