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엄기남(Ki Nam Eom),임승욱(Seung Ug Lim),노영수(Young Su Rho),송경철(Kyung Chul Song),이종민(Jong Min Lee),이지성(Jee Sung Lee),박찬용(Chan Yong Park),이의돈(Eui Don Lee),최유덕(Yu Duk Choi),하승연(Seung Yeon Ha) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.10
N/A Objective : The aim of this study was to evaluate the clinical experiences of patients with the primary tubal cancer diagnosed and treated in the Department of Obstetrics and Gynecology, Gacheon Medical School from January 1996 to December 2000. Methods : Age, symptoms, Pap smear, CA-125, preoperative diagnosis, mode of surgery, stage, additional pathologic finding, mode of adjuvant therapy and survival periods of patients were reviewed retrospectively. Results : The mean age of patients was 57.4 years. The symptoms were vaginal spotting, leukorrhea and pelvic mass. Three patients were treated by simple hysterectomy and both salpingoophorectomy. Staging laparotomy was done in 4 patients. Three patients were in stage I, 2 patients were in stage II and 2 patients were in stage III. Endometrial adenocarcinoma was present in 1 patient and endometrial hyperplasia was present in 1 patient. The mean follow-up period of five patients were 25.4 months and 2 patients were lost for follow-up. Conclusions : The primary tubal cancer was rare gynecologic malignancy and the preoperative diagnosis was difficult due to non-specific symptoms and signs. Staging laparotomy and adjuvant chemotherapy should be done like in ovarian cancer. More studies may be needed for the associated endometiral diseases.
이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희,Lee, Seung-Goo,Ro, Hyeon-Su,Hong, Seung-Pyo,Lee, Kyu-Jong,Wang, Ji-Won,Tae, Dong-Nyeon,Uhm, Ki-Nam,Bang, Sang-Gu,Kim, Young-Jun,Sung, Moon-Hee 한국미생물 · 생명공학회 1996 한국미생물·생명공학회지 Vol.24 No.1
By using the ${\beta}$-tyrosinase of Citrobacter freundii KCT2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18$^{\circ}C$ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammoniumacetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300mM and 50mM, respectively. Although pyrocatechol showed the optimal concentration at 20mM, it was controlled between 20mM and 50mM to avoid the depletion of substrate during the enzymatic synthesis. Meanwhile the synthetic rate was improved about 20% when ethanol was included in the reaction solution. Based on above results, a reaction medium for the productin of L-DOPA was prepared and incubated with 1 unit/ml of ${\beta}$-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solutin intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6g/l of L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.
제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산
이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1
재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.
안현경 ( Hyun Kyung Ah ),서은정 ( Eun Jeong Seo ),최영진 ( Young Jin Choi ),엄기남 ( Ki Nam,Eon ),이종민 ( Jong Min Lee ),이지성 ( Jee Seong Lee ),이순표 ( Soon Pyo Lee ),박찬용 ( Chan Yong Park ),최유덕 ( Yoo Deok Choi ),이의돈 ( 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.1
목적 : 미세침윤성 자궁경부암에서 침윤 정도에 따른 조직학적 특징, 치료 방법 및 합병증과 임상적 특징의 상관 관계를 평가하여 적절한 치료 방향을 설정하고자 하였다. 연구 방법 : 1997년 1월 1일부터 2001년 12월 31일까지 가천의대 길병원 산부인과에서 1994년 FIGO 병기에 따라 미세침윤성 자궁경부암으로 진단 받은 137명의 환자를 대상으로 원추생검술, 제1형, 제2형 및 제3형 자궁절제술을 시행하여 침윤 깊이를 1 mm 미만, 1-3 m Objective : To evaluate correlation the of histological variables and the clinical features of microinvasive cervical cancer with the depth of invasion and to establish the adequate therapeutic modality. Methods : One hundred and thirty-seven patients wit
폐경에 따른 자궁경부 세포진 검사상 AGUS 의 임상 분석
석원익(Won Ik Seok),이광범(Kwang Byum Lee),이종민(Jong Min Lee),엄기남(Gi Nam Um),윤성준(Seong Jun Yoon),최수란(Soo Lan Choi),김석영(Seok Young Kim),김광준(Kwang Jun Kim),이순표(Soon Pyo Lee),이지성(Jee Seong Lee),박찬용(Chan Yong Pa 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.6
목적 : 폐경상태에 따른 자궁경부 세포진 검사상 AGUS의 조직학적 결과 및 진단적 접근방법의 차이에 대한 임상적 중요성을 평가하고자 하였다. 방법 : AGUS로 진단된 104명 중 추적관찰이 가능했던 87명을 대상으로 폐경상태에 따른 조직학적 결과 및 진단 방법에 대한 평가를 하였다. 결과 : AGUS로 진단된 87명 중 폐경기 이전의 환자와 폐경 후 환자는 각각 46예 (52.9%)와 41예 (47.1%) 이었다. 87명중 70명 (80.5%)에서 중요한 조직학적 소견을 보였고, 이중 침윤성 질환을 보인 폐경기 이전 및 폐경 후 환자는 각각 19명 (41.3%)과 31명 (75.6%)으로, 폐경 후 환자에서 폐경기 이전 환자에 비하여 침윤성 암종이 유의하게 많이 관찰되었다 (P=0.002). 또한 진단방법의 평가에 있어 폐경 후 환자에서 폐경기 이전 환자에 비하여 제2군의 검사가 통계적으로 유의하게 많이 필요하였다 (P=0.018). 결론 : 자궁경부 세포진 검사상 AGUS는 추후의 조직검사에서 비침윤성 및 침윤성 병변의 높은 빈도와 관련되어 있다. 특히 폐경 후 환자에서는 제 1군의 검사 후 설명되지 않는 AGUS 환자에서 자궁경 및 자궁내막 병변의 잠재성의 병리상태 뿐만 아니라 드물지만 위장관, 난관, 난소 또는 유방 등 자궁 이외의 병변의 가능성을 배제하기 위해 보다 적극적인 검사가 추천된다. Objective : To evaluate the clinical significance of AGUS including histologic results and diagnostic modalities according to menopausal status. Methods : One hundred and four patients with AGUS were identified. Corresponding biopsies were available for 87 of these cases. The evaluation for histologic results and diagnostic modalities was made according to menopausal status. Results : Among the 87 patients with AGUS, the number of premenopausal and menopausal patients were 46 (52.9%) and 41 (47.1%), respectively. Over all, 70 (80.5%) of 87 patients were found to have important histologic findings and of them, premenopausal and menopausal patients with invasive diseases were 19 (41.3%) and 31 (75.6%) respectively. There were significant differences in pathologic findings between premenopausal and menopausal patients indicating more invasive lesions in menopausal than premenopausal patients (p=0.002). For the evaluation of diagnostic modality, the second arm was significantly more needed for menopausal than premenopausal patients (p=0.018). Conclusion : AGUS on pap smear represents a cytologic diagnosis associated with high incidence of underlying preinvasive and invasive lesions, especially in postmenopausal patients. Thus aggressive work-up is recommended to rule out the potential pathologic conditions in endocervix, endometrium and rarely extrauterine lesions such as gastrointestinal, tubal, ovarian or breast in patients otherwise unexplained AGUS after careful first arm work-up.
임신중기 원인불명의 융모성 성선자극호르몬 상승과 임신예후와의 연관성
송경철(Kyung Chul Song),이지성(Ji Sung Lee),임승욱(Seung Ug Lim),엄기남(Gi Nam Eom),강철규(Cheol Gyu Kang),최유덕(Yu Duk Choi),김석영(Sug Young Kim),황병철(Byoung Chul Hwang),김광준(Kwang Jun Kim),이의돈(Eui Don Lee),박찬용(Chan Yong 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8
N/A Objective : The purpose of this study was to compare the neonatal and maternal infectious morbidity between single and multiple courses of antenatal betamethasone treatment in patients with preterm premature rupture of membranes. Methods : One hundred seventy patients who delivered neonates between 28 and 34 weeks' gestation after preterm premature rupture of membranes from January 1992 to July 2000 were reviewed retrospectively. Patients were divided into 3 groups on the basis of the following betamethasone exposures: (1) none (control subjects), (2) betamethasone 4 mg IM, IV simultaneously and then 4 mg IV q 8 hours for 24 hours (single course) and (3) weekly administration after initial single course (multiple courses). All included patients received prophylactic antibiotics for group B streptococci. The statistical analyses were done using χ2 test, Fisher's exact test and one way analysis of variance (ANOVA). Multiple logistic regression analysis was performed to determine the confounding effect of the multiple variables those were considered as risk factors for neonatal sepsis. Results : This study included 67 patients in the control group, 60 patients in the single course group, and 43 patients in the multiple courses group. The latency (p=.0001) was significantly longer in the patients exposed to multiple course than the patients in the control group and those in the single course group. No significant difference was demonstrated in the incidence of neonatal sepsis (p=.881) and postpartum endometritis (p=.619) among the three groups. Neonatal sepsis was significantly associated with clinical chorioamnionitis (p=.022). Conclusion : According to our data, multiple courses of antenatal betamethasone treatment in patients with preterm premature rupture of membranes was not associated with the increased incidence of neonatal sepsis and postpartum endometritis.