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      • Bandom Amplified Polymorphic DNAs 기법을 이용한 Bifidobacteria의 신속한 동정

        왕지원,민해기,장영효,강국희 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

        Random Amplified Polymorphic DNAs (RAPD) has been applied to the identification of human intestinal anaerobic Bifidobacteria. Recently, The RAPD technique has been used to analyse DNA polymorphism of genomic DNA from eukaryotes such as plants and animals(1, 3, 4, 10, 11). But there were only several reports on the polymorphism of prokaryotic genomic DNA by using this method(2,7). Total 100 random sequence primers were used in screening of species-specific primer. The oligonucleotide primers were ten bases long and designed to have average G+C contents of 60 mol %. Eight species of Bifidobacteria from human feces were selected. According to conventional method genornic DNA were extracted from each bifidobacterium and then RAPD-PCR was performed. Four primers have produced distinct electrophoretic band patterns, RAPD markers. Especially arbitrary primer # 101 showed species-specific DNA polymorphism for each of Bifidobacterial genomic DNA tested. The arbitrary primer # 101 showed good reproducibility when the PCR conditions were optimized. And also primer # 156, # 174, # 183, have produced amplified PCR products but not for all of the strains tested. Better DNA polymorphism was observed when two arbitrary primers were used in combination.

      • Bifidobacterium angulatum KCTC3236의 α-galactosidase에 의한 Oligo당 생성의 최적조건

        왕지원,김경민,강대중,강국희,정재록 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

        Bifidobacterium angulatum KCTC3236 showed the highest α-galactosidase activity among the tested strains of Bifidobacteria( data not shown). Optimum culture conditions for the maximum production of α-galactosidase were as follows. The culture media was MRS broth containing 1% galactose as a carbon source and its initial pH was 7.0. The incubation temperature and time were 37℃, 21 hours respectively. Melibiose have been used as substrate. The formation of oligosaccharides was maximum at 40℃ when the substrate concentration was 30%. And the unit of α-galactosidase was 40 unit. Finally, we could observe the maximum production of oligosaccharides after 14 hours' enzyme reaction.

      • KCI등재

        중국 광역권역 대도시의 토지이용효율성에 관한 연구 : DEA분석을 중심으로

        왕지원,나주몽,경성림 한국동북아학회 2015 한국동북아논총 Vol.20 No.4

        본 연구에서는 중국의 12개 전형적인 주요 도시의 2008-2012년까지의 5년간 통계자료를 이용하여 각 도시의 토지이용효율성을 평가하였다. 동부권역·중부권역·서부권역 도시들의 토지이용효율성을 비교하기 위해 DEA 기법을 활용하여 각 도시별 기술효율성을 CCR모형으로, 순수기술효율성을 BCC모형으로, 규모의 효율성을 CCR/BCC로 측정하였다. 구체적으로 분석결과를 요약하면 다음과 같다. 첫째, 중국의 12개 도시의 연도별 기술적 효율성을 CCR 모형으로 분석한 결과, 상대적으로 서부지역은 동부지역과 중부지역보다 기술효율성이 상대적으로 낮게 분석되었다. 둘째, 순수 기술적 효율성(BCC)을 분석한 결과 동부권역의 상하이시, 톈진시, 광저우시와 베이징시는 비슷한 효율성을 보였으며, 중부권역의 경우 창사시가 100%로 정저우시、우한시와 허페이시보다 높은 효율성을 나타냈다. 서부권역의 경우 순수 기술적 효율성만을 본다면, 시안시, 충칭시, 청두시, 쿤밍시 등은 BCC 모형을 적용해도 각각 86.23%, 74.45%, 80.80%, 86.83%에 불과해 순수 기술적인 측면에서도 여전히 개선의 여지가 있는 것으로 분석되었다. 셋째, 규모의 효율성을 분석한 결과, 동부지역과 중부지역의 도시들이 서부지역의 도시들에 비해 효율적으로 나타나고 있으며, 서부지역의 평균 효율성 값들이 점진적인 증가세를 보이고 있다. 넷째, 상대적으로 서부권역의 도시들이 동부권역과 중부권역의 도시들보다 효율성이 떨어지는 것으로 나타났다. Land-use is the process of long term or periodical management of land for mankind's socioeconomic goal through utilizing a chain of living things and technological activity. it has a strong relationship with humans' quality of life and sustainable development. Research on land-use efficiency of cities is thus extremely important. In this research, to evaluate cities' land-use efficiency, a widely used efficiency analysis, the DEA method, was applied to 12 archetypal major cities in eastern, central and western China and their land-use efficiency was analysed. Firstly, results of the year-on-year technical efficiency of China's 12 cities analyzed using a CCR model indicate that the technical efficiency of the western region was relatively lower than those of the eastern and central regions. Secondly, results of scale efficiency measured through CCR/BCC showed cities in eastern and central regions to be more efficient than those in the western region while for the western region mean efficiency values display a gradually increasing trend as they approach recent years.

      • KCI등재

        문화지능이 직무성과에 미치는 영향: 다양성 관리의 조절효과를 중심으로

        왕지원,정수진 한국경영교육학회 2019 경영교육연구 Vol.34 No.5

        [Purpose]This study examines the effect of cultural intelligence on job performance in a cross-cultural environment and examines the moderating effects of diversity management on the relationship between cultural intelligence and job performance. It aims to raise the importance of cultural intelligence in the global age and to use appropriate diversity management to improve organizational performance in a cross-cultural environment. [Methodology]Based on previous studies, the theoretical background of cultural intelligence, job performance, and diversity management was demonstrated and hypotheses were established. The measurement was performed SPSS 23.0 for the analysis method, which used the techniques of factor analysis, correlation analysis, and regression analysis. [Findings]Through research analysis, cultural intelligence was found to have a significant positive effect on job performance. Diversity management was found to have a partial moderating effect on the relationship between cultural intelligence and job performance. [Implications]According to the results of research, cultural intelligence has a significant meaning in job performance in a cross-cultural environment. In practice, it has been found that it can improve the cultural intelligence of the members of the organization and increase the performance of the job. [연구목적] 본 연구는 이문화 환경에서 문화지능이 직무성과에 미치는 영향을 검증하고 다양성 관리가 문화지능과 직무성과의 관계에서의 조절효과를 살펴보는 것이다. 글로벌 시대에 문화지능의 중요성을 제기하고 이문화 환경에서 조직성과를 향상시키기 위해 적합한 다양성 관리를 이용하는 데에 목적이 있다. [연구방법] 선행연구를 토대로 문화지능, 직무성과와 다양성 관리의 이론적 배경을 논증하고 가설을 설정한 다음에 연구모형을 구축하였다. 분석방법은 SPSS 23.0을 사용하여 요인분석, 상관관계분석과 회귀분석의 기법을 활용하였다. [연구결과] 연구 분석을 통해 문화지능이 직무성과에 유의적인 정(+)의 영향을 미치는 것으로 검증되었다. 다양성 관리는 문화지능과 과업성과의 관계에서 유의적인 조절효과를 확인되었고 문화지능과 맥락성과의 관계에서 부분적 조절효과를 미치는 것을 확인되었다. [연구의 시사점] 연구의 결과에 따라 이문화 환경에서 문화지능이 직무성과에 중요한 의미를 갖는다. 실무적으로 조직 구성원의 문화지능을 향상시키고 직무성과를 높일 수 있는 것을 확인되었다. 동시에 제도적 다양성 관리 방법을 이용하여 전략적 다양성 방법을 제정하고 문화지능을 향상시켜 직무성과와의 적합한 형태를 제시하였다.

      • KCI등재

        Effects of Progressive Core and Ankle Muscle Strengthening Exercises Using Thera-Band on Body Balance

        이광섭,왕지원,이동엽,유재호,김진섭,김성길,홍지헌 대한물리치료학회 2022 대한물리치료학회지 Vol.34 No.3

        Purpose: This study aims to compare the effect on balance during core and ankle muscle strengthening exercises using the Thera-Band. Methods: 21 healthy college students were recruited. The participants were divided into a core strengthening group (CSG), an ankle strengthening group (ASG), and a non-exercise group (NEG). CSG and ASG were performed twice a week for a total of 4 weeks, and static and dynamic balance were measured before and after the intervention. The static balance were measured as stability index and weight distribution index using Tetrax®. The dynamic balance was measured in each direction by the Y balance test. The Thera-Band intensity was increased after 2 weeks of exercise, and the exercise was subsequently performed. Results: Participants showed static balance with Tetrax®, a significant difference was noted between normal eye closes and pillow with eye closes in ASG (p<0.05). In the case of dynamic balance with the Y balance test, a significant difference was observed in posterolateral direction (PL) and composite score (CS) between each group for the pre- and post-intervention differences (p<0.05). A significant difference was observed between PL and CS in CSG (p<0.05). Conclusion: These findings show that the progressive Thera-Band exercise had a positive effect on balance abilities. It was confirmed that core strengthening was effective for dynamic balance, and ankle strengthening was effective for static balance.

      • Limulus Amoebocyte Lysate법에 의한 원유와 살균유의 세균수 측정

        姜國熙,王智怨,朴興洙,李圭鍾,金敬民 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.2

        LAL method for the determination of Gram-negative bacterial Lipopolysaccharides(LPS) in milk is proposed and its value for bacteriological quality control is investigated. The relationship between the LPS contents and total colony count(cfu/ml), which covers the Gram-negative bacteria, was determined in raw milk samples. The coefficient of correlation between Gram-negative bacterial counts(cfu/ml) and LPS contents was r^2 =0.969. And, the correlation coefficient between total bacterial counts(cfu/ml) and LPS contents was r^2 =0. 914. It was also investigated how much the LPS contents were in UHT milks from l0 different dairy industrial companies in Korea. It varied from 92 ng LPS/ml to 938 ng LPS /ml.

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