Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

Biomechanical analysis of lumbar decompression surgery in relation to degenerative changes in the lumbar spine – Validated finite element analysis

Li, Quan You,Kim, Ho-Joong,Son, Juhyun,Kang, Kyoung-Tak,Chang, Bong-Soon,Lee, Choon-Ki,Seok, Hyun Sik,Yeom, Jin S. Elsevier 2017 Computers in biology and medicine Vol.89 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>There are no studies about the biomechanical analysis of lumbar decompression surgery in relation to degenerative changes of the lumbar spine. Therefore, the purpose of this study was to compare, by using finite element (FE) analysis, the biomechanical changes of the lumbar spine in terms of annulus stress and nucleus pressure after two different kinds of lumbar decompression surgery in relation to disc degenerative changes.</P> <P><B>Methods</B></P> <P>The validated intact and degenerated FE models (L2-5) were used in this study. In these two models, two different decompression surgical scenarios at L3-4, including conventional laminectomy (ConLa) and the spinous process osteotomy (SpinO), were simulated. Therefore, a total of six models were simulated. Under preloading, 7.5 Nm moments of flexion, extension, lateral bending, and torsion were imposed. In each model, the maximal von Mises stress on the annulus fibrosus and nucleus pressure at the index segment (L3-4) and adjacent segments (L2-3 and L4-5) were analyzed.</P> <P><B>Results</B></P> <P>The ConLa model and disc degeneration model demonstrated a larger annulus stress at the decompression level (L3-4) under all four moments than were seen in the SpinO model and healthy disc model, respectively. Therefore, the ConLa model with moderate disc degeneration showed the highest annulus stress at the decompression level (L3-4). However, the percent change of annulus stress at L3-4 from the intact model to the matched decompression model was less in the moderate disc degeneration model than in the healthy disc model.</P> <P><B>Conclusions</B></P> <P>Although the ConLa model with moderate disc degeneration showed the highest annulus stress, the degenerative models would be less influenced by the decompression technique.</P>

The Roles of the Government in Developing "Sport for All Movement" in Korea: From Intervention / Control to Accommodation / Support

( Ju Ho Chang ),( Seong Sik Cho ) 한국스포츠정책과학원(구 한국스포츠개발원) 2000 International Journal of Applied Sports Sciences Vol.12 No.1

This study attempts to figure out the roles of the government in developing ``sport for all movements`` which from intervention/control to accommodation/support. It discusses the relationships between the development of sport for all and the governmental roles in Korea in terms of political, economic and societal changes. It points out that the governmental intervention and control were the basic orientation of sport-policies when the civil sectors of society and local autonomous administration were underdeveloped and the political regime was authoritarian. With the development and democratization of the civil society and politics, however, this study stresses that non-governmental organizations and local autonomous governments tend to take on the responsibility for the sport for movement. With regard to societal changes, the roles of the government should be more toward accommodation and support rather than intervention and control. This study argues that the expansion and growth of civil sectors will increase the diversification, effectiveness, and efficiency of the sport for all movement and that the increase in the functions and roles of the local governments will help to mobilize the local resources and community-based sport for all movement. Finally, it suggests the strong partnership relationships between the civil sectors and the government, and between the local and the national governments based upon mutual trust and cooperation.

소의 대동맥 내피세포에서 산화저밀도 지단백에 의한 아포프토시스에 백일해 독소 및 콜레라 독소가 미치는 영향

손호영(Ho Young Son),강성구(Sung Ku Kang),강무일(Moo Il Kang),차봉연(Bong Youn Cha),이광우(Kwang Woo Lee),장상아(Sang Ah Chang),최윤희(Yoon Hee Choi),문성대(Sung Dae Moon),안유배(Yoo Bae Ahn),손현식(Hyun Sik Son),윤건호(Kun Ho Yoon) 대한내과학회 1999 대한내과학회지 Vol.56 No.5

N/A Objectives : Oxidized LDL is thought to play a key role in atherogenesis. Among their wide variety of biological properties, oxidized LDL injures the endothelium as an early event of atherogenesis. However, the mechanisms by which oxidized LDL injures endothelial cells are not definitely known. In order to evaluate the involvement of GTP-binding protein on the mechanism of apoptosis, we studied the effects of pertussis and cholera toxin on oxidized LDL-induced apoptosis in bovine aortic endothelial cells(BAECs). Methods : Oxidized LDL elicited apoptosis in cultured BAECs as shown by characteristic morphological and biochemical changes. Chromatin condensation and nucleus fragmentation were visualized by using fluorescence microscopy of intact cells staining by acridine orange/ ethidium bromide. DNA fragmentation was quantified by an ELISA with specific antibody for bromodeoxyuridine- labelled DNA fragments and confirmed with DNA ladder formation. Results : Studies using a combination of bacterial toxins which change the function of GTP-binding protein suggest that oxidized LDL-induced apoptosis was regulated by GTP-binding protein. Oxidized LDL-induced apoptosis was not changed by pretreatment of BAECs with pertussis toxin. In contrast, pretreatment with cholera toxin completely prevented the oxidized LDL- induced apoptosis. Conclusion : These results show that oxidized LDL induces apoptosis of BAECs and suggest that cholera toxin-sensitive G-proteins are involved in signal transduction of oxidized LDL-induced apoptosis of BAEC.

특급 호텔 식음료 이벤트와 이미지에 관한 실증적 연구

우경식(Kyoung Sik Woo),이창호(Chang Ho Lee) 한국호텔외식관광경영학회 2001 호텔경영학연구 Vol.10 No.1

The purpose of this study is to set up the direction and main theme of further studies and raise the usefulness of such studies to be helpful to hotel practices by clarifying what effects hotel events have on hotel image. Recently the opportunity to discriminate hotel businesses has been reduced because the hotel industry has shared cross-cultural products and ideas and provided similar products and services to customers, under the development of service industry and information. As an alternative solution to this problem. many hotels are putting dynamic elements on their products and services and activating events as means for stimulating purchase desires and promoting sales. This suggests that hotel F&B events have a variety of factors which can stimulate or change existing hotel images and then become motives for purchasing or selection hotel products. The major findings and implications can be summarized as follows; Concerning variables of hotel image, hotels whose images changed much showed excellence in the menu creativity, event rationality. Among those attributes of hotel events, menu creativity was found most influencing hotel image changes. Thus the menu creativity of hotel event should be strengthened to maintain re-purchase by customers participating in hotel events. In terms of customers` age, those who are in their 20s thought facility functionality as relatively important while those who are in their 30s rich atmosphere. 40s and 50s or over special entertainment and menu creativity. Therefore it is also needed to make hotel event planning considering customers` age and lifestyle.

농작물을 위한 드론 분무 농약 살포의 3차원 분석에 관한 연구

문인식(In Sik Moon),권현진(Hyun Jin Kown),김미현(Mi Hyeon Kim),장세명(Se Myong Chang),나인호(In Ho Ra),김흥태(Heung Tae Kim) 한국스마트미디어학회 2020 스마트미디어저널 Vol.9 No.4

농작물들의 크기와 형태는 매우 다양하며 생육 환경도 각기 다르다. 따라서 드론을 활용하여 농약을 살포할 때에는 각 농작물에 대한 재배 환경과 특성이 고려되어야 하며, 이에 따라 드론의 비행고도, 전진속도 등 비행 조건이 달라져야 한다. 실제로 비행 조건에 따라 농약의 액적 유동이 크게 영향을 받게 되며, 살포 영역에 큰 변화가 발생하고 이로 인해 불균일한 액적 분포가 후류에 형성되어 농약의 전달 효율성이 떨어질 수 있을 뿐만 아니라 비산에 대한 위험성이 존재하게 된다. 본 논문에서는 농업용 드론을 사용하여 특성이 다른 3가지 농작물을 선정하고 드론의 비행 조건을 각각 다르게 하여 농약을 살포했을 때 후류에서의 노즐 유동을 수치해석을 통하여 분석하였으며, 전달되는 액체의 비율을 확률 밀도 함수의 평균 제곱근을 나눈 새로운 성능지수를 이용하여 비교함으로써 작물의 특성에 따른 드론의 농약 살포 가이드라인을 구축하고자 한다. The size and shape of crops are diverse, and the growing environment is also different. Therefore, when one uses a drone to spray pesticides, the characteristics of each crop must be considered, and flight conditions such as the flight height and forwarding velocity of the drone should be changed. The droplet flow of pesticides is affected by various flight conditions, and a large change occurs in the sprayed area. As a result, an uneven distribution of liquid may be formed at the wake, and the transport efficiency will be decreased as well as there would be a risk of toxic scatter. Therefore, this paper analyzes the degree of distribution of pesticides to the crops through numerical analysis when pesticide is sprayed onto the selected three crops with different characteristics by using agricultural drones with different flight conditions. On the purpose of establishing a guideline for spraying pesticides using a drone in accordance with the characteristics of crops, this paper compares the amount of pesticides distributed in the crops at the wake of nozzle flow using the figure of merit, and the sum of transported liquid rate divided by the root mean square of the probability density function.

급성 췌장염으로 발현된 낭성 변화를 이릉킨 종격동 부갑상선 선종 1예

이준호,최영식,박진홍,허갑도,윤설영,최소진,권태헌,이창렬,손성표,박영효 대한내분비학회 1998 Endocrinology and metabolism Vol.13 No.3

Hypercalcemia due to hyperparathyroidism is rarely associated with acute pancreatitis. But, the relationship between hypercalcemia and pancreatitis still remains controvesial. Ectopic parathyroid adenoma with cystic change is one of the rare causes of hyperparathyroidism, and is usually located in neck and mediastinum. We report a case of mediastinal parathyroid adenoma with cystic change associated with acute pancreatitis. A 54-year-old male presented with epigastric pain for 3 days. The serum calcium, phosphate, elastase were 16.8mg/dL, 1.1 mg/dL, 2772.0 ng/mL respectively and his parathyroid hormone level in serum was 651.84 pg/mL. Chest CT showed a mediastinal mass with well defined inhomogenously enhanced density located between SVC and aorta. The patient was diagnosed to hyperparathyroidism with acute pancreatitis due to mediastinal parathyroid adenoma with cystic change, and was surgically removed. The surgical biopsy showed parathyroid adenoma with cystic change. After operation his general condition was improved and serum calcium, phosphate, amylase, lipase level were normalized (J Kor Soc Endocrinol 13:473-479, 1998).

Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

Choi, Eun Sik,Lee, Yun Jung,Seo, Chang Seob,Yoon, Jung Joo,Han, Byung Hyuk,Park, Min Cheol,Kang, Dae Gill,Lee, Ho Sub Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>Samul-Tang (Si-Wu-Tang, SMT), composed of four medicinal herbs, is a well-known herbal formula treating hematological disorder or gynecologic disease. However, vascular protective effects of SMT and its molecular mechanisms on the vascular endothelium, known as the central spot of vascular inflammatory process, are not reported. The aim of this study was to investigate vascular protective effects of SMT water extract in human umbilical vein endothelial cells (HUVECs). Water extract of SMT was prepared and identified by HPLC-PDA analysis. Expression of cell adhesion molecules (CAMs) and heme oxygenase-1 (HO-1) and translocation of nuclear factor-kappa B (NF-<I>κ</I>B) and nuclear factor-erythroid 2-related factor 2 (Nrf2) were determined by western blot. Nuclear localization of NF-<I>κ</I>B and Nrf2 was visualized by immunofluorescence and DNA binding activity of NF-<I>κ</I>B was measured. ROS production, HL-60 monocyte adhesion, and intracellular nitric oxide (NO) were also measured using a fluorescent indicator. SMT suppressed NF-<I>κ</I>B translocation and activation as well as expression of CAMs, monocyte adhesion, and ROS production induced by TNF-<I>α</I> in HUVECs. SMT treated HUVECs showed upregulation of HO-1 and NO which are responsible for vascular protective action. Our study suggests that SMT, a traditionally used herbal formula, protects the vascular endothelium from inflammation and might be used as a promising vascular protective drug.</P>

만성 췌장염에 의한 십이지장 협착 1예

서백종,장기육,박용근,채현석,한석원,이창돈,정인식,차상복,박두호 대한내과학회 1997 대한내과학회지 Vol.53 No.3S

저자들은 만성췌장염환자에서 지속적인 오심과 구토를 주소로 내원하여 십이지장협착으로 진단된 1예를 경험하였기에 보고하는 바이다. Duodenal stenosis resulting from fibroinflammatory process is a rare complication of chronic pancreatitis. The mechanism of duodenal stenosis seems to be related to ischemia and edema caused by persistent inflammation, which results in irreversible change of duodenal wall structure. We experienced a chronic alcoholic male patient with duodenal stenosis who had a history of choledochojejunostomy to relieve distal bile duct stenosis several years ago. He had suffered persistent postprandial nausea and vomiting for several months. Barium study of upper gastrointestinal tract and repeated endoscopy show narrowing lumen of duodenum with coarse nodular surface due to reactive inflammatory change.

맥류 연구의 과거, 현재, 그리고 미래

김경민(Kyeong-Min Kim),강천식(Chon-Sik Kang),김양길(Yang-Kil Kim),김경훈(Kyeong-Hoon Kim),박종호(Jong-Ho Park),윤영미(Young-Mi Yoon),박형호(Hyoung-Ho Park),정한용(Han-Yong Jeong),최창현(Chang-Hyun Choi),박진희(Jinhee Park),김영진(You 한국육종학회 2020 한국육종학회지 Vol.52 No.S

Barley and wheat are a major food crop of humans, along with rice, soybean and corn. A systematic breeding program for Korean barley began in 1906 with selection and introduction breeding. In 1908, landrace barley was collected, and breeding focused on selection and introduction for high yielding varieties until the 1970s. In the 1980s and 1990s, breeding was carried out for diversity; thereafter, we aimed to improve quality, productivity and lodging tolerance that can be applied to the paddy field in Korea. Since 2010, the major result of breeding was shortening cultivation to approximately 6–12 days, making a double cropping system possible in the paddy field in Korea. Yield has increased by 1.2 times from 438 kg/10 a in the 1960s to 536 kg/10 a in the 2010s, and farm yield has increased by 1.7 times. In addition, as cultivation safety has been enhanced, the varieties have also improved, such as the covered barley used in making tea and other processing products. In case of wheat, up to now forty cultivars have been developed in Korea. In the early stage, we developed a domestic wheat variety that was early maturing and high yielding. As a result, the maturation time of wheat planted in the 1970s to 2010 was shortened (from 13 to 30 days), while productivity increased by 30% from 408 kg/10 a in the 1970s to 532 kg/10 a in 2010. In recent years, there have been remarkable efforts for a more stable production by focusing on increasing disaster and pest resistance due to climate change. In addition, a wheat variety discrimination marker was developed using a variety-specific marker, and selection was made using a trait-specific marker at the early stage of breeding to enhance breeding efficiency. In the 2000s, winter cereals for forage have been promoted to expand forage production and to replace imported feed grains. Therefore, winter cereal that is useful for feed, such as rye, oat, and triticale, have had various varieties and safe production techniques developed. Currently, our research goal for winter cereals for feed is to develop a double-cropping adaptation and abiotic stress tolerance cultivar, and safe production in paddy field. Hence, aggressive action is needed to support the strategic survival of the Korean wheat and barley industry. Barley is a health food that requires a multifaceted effort to improve breeding efficiency, develop varieties that contain large amounts of functional components and are more resistant to stronger biotic and abiotic stresses in response to climate change. It is necessary to recognize the role of wheat and barley as the second main crop after rice, and to improve the self-sufficiency rate of these crops for the health and food industry crisis of Korea.