Fabrication and formation mechanism of double-layer coating by plasma electrolytic oxidation with particle addition

Shuqi Wang,Yongchun Zou,Guoliang Chen,Zhiyun Ye,Yaming Wang 한국표면공학회 2023 한국표면공학회 학술발표회 초록집 Vol.2023 No.11-1

High Emissivity Ceramic Coatings Fabricated on Light Metals by PEO: Microstructure and Heat Dissipation Property

Yaming Wang,Shuqi Wang,Yongchun Zou,Guoliang Chen 한국표면공학회 2023 한국표면공학회 학술발표회 초록집 Vol.2023 No.11-1

Comparative transcriptome analysis of salt-sensitive and salt-tolerant maize reveals potential mechanisms to enhance salt resistance

Mingquan Wang,Yufeng Wang,Yifei Zhang,Chunxia Li,Shichen Gong,Shuqin Yan,Guoliang Li,Guanghui Hu,Honglei Ren,Jianfei Yang,Tao Yu,Kejun Yang 한국유전학회 2019 Genes & Genomics Vol.41 No.7

Background Salt stress is a devastating environmental stress that causes plant growth inhibition and yield reduction. Objective The identification of salt-tolerant genes brings hope for the generation of salinity-tolerant crop plants through molecular breeding. Methods In this study, one salt-sensitive and one salt-tolerant maize inbred line were screened from 242 maize inbred lines. Reactive oxygen species (ROS)-related enzyme activities were detected and salt-responsive comparative transcriptome analysis was performed for control and 220 mM NaCl treated maize leaves. Results Salt-tolerant maize inbred line (L87) showed higher ROS-related enzyme (SOD, POD, APX and CAT) activities and accumulated relatively lower levels of ROS under salt stress. Of the total DEGs, 1856 upregulated DEGs were specific to L87, including stress tolerance-related members of the 70kDa family of heat shock proteins (Hsp70s) and aquaporins. The DEGs involved in the abscisic acid (ABA), ethylene, jasmonic acid (JA) and salicylic acid (SA) signal transduction pathways may determine the difference in salt tolerance between the two varieties, especially one central component SnRK2, that positively regulates ABA signaling and was only upregulated in L87. Analysis of DEGs related to ROS scavenging showed that some peroxidase (POD), glutathione S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) genes specific to L87 probably enhanced its salt tolerance. The analysis of differentially expressed transcription factors (TFs) suggested that WRKY TFs could contribute to the difference in salt tolerance between the two maize lines. Conclusion Compared with Salt-sensitive maize inbred line (L29), L87 exhibits specific regulatory mechanisms related to salt tolerance, including plant hormone interactions, ROS scavenging and the regulation of TFs. Our study identifies new candidate genes that may regulate maize tolerance to salt stress and provides useful information for breeding maize with high salt resistance.

Functional Analysis of Wheat TaPaO1 Gene Conferring Pollen Sterility Under Low Temperature

Changping Zhao,Guoliang Yuan,Yukun Wang,Shaohua Yuan,Peng Wang,Wenjing Duan,Jianfang Bai,Hui Sun,Na Wang,Fengting Zhang,Liping Zhang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.1

Thermosensitive male sterility plays an important role in wheat fertility and production. As a key enzyme for chlorophyll degradation, pheophorbide a oxygenase (PaO) can suppress cell death in plants. We cloned the wheat gene TaPaO1 from the thermosensitive genetic male sterile (TGMS) line BS366; it encodes a typical PaO protein, containing a conserved Rieske [2Fe-2S] iron–sulphur motif, a mononuclear non-heme iron-binding motif, and a C-terminal CxxC motif. TaPaO1 was expressed in all tissues and was upregulated during the meiosis stage of BS366 anthers under low temperature. Subcellular localization of TaPaO1 specifically labelled the surrounding of chloroplasts. TaPaO1 regulated by RD29A promoter which responded to low temperature led to pollen sterility in transgenic tobacco. Expression analysis showed that TaPaO1 exhibited a higher level of expression in the anther than in other tissues in transgenic tobacco plants during low temperature treatment. We propose that the higher senescence-related activity of TaPaO1 may lead to the cell death of anthers, which happens at an early developmental stage under low temperature. These results provide new insights into the function of PaO during the early developmental stage of anthers. PaO is closely related to cell death regardless of whether it exhibits increased activity or inactive.

Baicalein induces the apoptosis of U251 glioblastoma cell lines via the NF-kB-p65- mediated mechanism

Guoliang Jiang,Lei Zhang,Jinkun Wang,Houjun Zhou 한국통합생물학회 2016 Animal cells and systems Vol.20 No.5

Glioblastoma (GBM) is the most aggressive cerebral gliomas. Moreover, the overall prognosis of GBM is still little. Baicalein (BA) is a flavonoid derived from the Scutellaria baicalensis root, and has historically been used in anticancer therapies. However, its apoptosis role and related mechanisms in GBM has not yet been researched clearly. Thus, this study aimed to investigate the effects of BA on human GBM U251 cell line. The effects of BA on proliferation of U251 cells were measured by Cell Counting Kit-8 assay. Cellular apoptosis was detected by flow cytometry with annexin V-FITC/propidium iodide staining. The expression of apoptosis-related protein Bcl-2, Bax and cleaved-caspase3 was detected by quantitative real-time PCR and western blot. The expression of nuclear p65 protein, the active subunit of nuclear factor-kappa B (NF-κB), was determined by immunofluorescence and western blot. Our results showed that the viability of U251 cells significantly decreased in a time- and dose-dependent manner after treated with BA, and the apoptotic ratio of BA-treated groups was significantly higher than that of control groups. Furthermore, the expression of NF-kB-p65 in the nucleus was remarkably reduced, and the activity of NF-kB-p65 was remarkably inhibited after BA treatment. Combined treatment with a NF-kB-P65 inhibitor (QNZ) and BA resulted in the synergistic reduction of Bcl-2 expression and then increase of Bax and cleaved-caspase3 expression; and the viability of U251 cells was also inhibited. In conclusion, BA inhibits GBM cells viability and induces apoptosis via inhibit the activity of NF-kB-p65, suggesting that BA is a potential therapeutic agent for GBM.

The Computer Control On Laser Pulse Energy In Precise Laser Machinings

Wang Xiaodong,Sun Kun,Piao Zhongming,Yuan Guoliang,Xin deseng,Dong Hui 대한전자공학회 1991 ICEIC:International Conference on Electronics, Inf Vol.1991 No.8

Observer-based Extended Dissipative Control for Delayed Singular Markovian Jump Systems via Relaxed Lyapunov-Krasovskii Functional

Xin Wang,Guangming Zhuang,Jianwei Xia,Guoliang Chen,Huasheng Zhang 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.12

This paper addresses the problem of observer-based extended dissipative control for singular Markovian jump systems with time-varying delays. By using relaxed Lyapunov-Krasovskii functional approach and strict linear matrix inequality method, sufficient conditions are proposed to ensure that the delayed singular Markovian jumping system is stochastically admissible and extended dissipative. Based on these conditions, a desired observer-based memory state feedback controller is designed to ensure that the closed-loop system satisfies stochastic admissibility and extended dissipativity. Finally, two examples including a numerical example and an oil catalytic cracking process are provided to illustrate the effectiveness of the proposed methods.

Intensity of Intraoperative Spinal Cord Hyperechogenicity as a Novel Potential Predictive Indicator of Neurological Recovery for Degenerative Cervical Myelopathy

Chen Guoliang,Wei Fuxin,Li Jiachun,Shi Liangyu,Zhang Wei,Wang Xianxiang,Xu Zuofeng,Liu Xizhe,Zou Xuenong,Liu Shaoyu 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.7

Objective: To analyze the correlations between intraoperative ultrasound and MRI metrics of the spinal cord in degenerative cervical myelopathy and identify novel potential predictive ultrasonic indicators of neurological recovery for degenerative cervical myelopathy. Materials and Methods: Twenty-two patients who underwent French-door laminoplasty for multilevel degenerative cervical myelopathy were followed up for 12 months. The Japanese Orthopedic Association (JOA) scores were assessed preoperatively and 12 months postoperatively. Maximum spinal cord compression and compression rates were measured and calculated using both intraoperative ultrasound imaging and preoperative T2-weight (T2W) MRI. Signal change rates of the spinal cord on preoperative T2W MRI and gray value ratios of dorsal and ventral spinal cord hyperechogenicity on intraoperative ultrasound imaging were measured and calculated. Correlations between intraoperative ultrasound metrics, MRI metrics, and the recovery rate JOA scores were analyzed using Spearman correlation analysis. Results: The postoperative JOA scores improved significantly, with a mean recovery rate of 65.0 ± 20.3% (p < 0.001). No significant correlations were found between the operative ultrasound metrics and MRI metrics. The gray value ratios of the spinal cord hyperechogenicity was negatively correlated with the recovery rate of JOA scores (ρ = -0.638, p = 0.001), while the ventral and dorsal gray value ratios of spinal cord hyperechogenicity were negatively correlated with the recovery rate of JOA-motor scores (ρ = -0.582, p = 0.004) and JOA-sensory scores (ρ = -0.452, p = 0.035), respectively. The dorsal gray value ratio was significantly higher than the ventral gray value ratio (p < 0.001), while the recovery rate of JOA-motor scores was better than that of JOA-sensory scores at 12 months post-surgery (p = 0.028). Conclusion: For degenerative cervical myelopathy, the correlations between intraoperative ultrasound and preoperative T2W MRI metrics were not significant. Gray value ratios of the spinal cord hyperechogenicity and dorsal and ventral spinal cord hyperechogenicity were significantly correlated with neurological recovery at 12 months postoperatively.

Comparative digestibility of nutrients and amino acids in high-fiber diets fed to crossbred barrows of Duroc boars crossed with Berkshire×Jiaxing and Landrace×Yorkshire

Zhao, Jinbiao,Wang, Qiuyun,Liu, Ling,Chen, Yiqiang,Jin, Aiming,Liu, Guoliang,Li, Kaizhen,Li, Defa,Lai, Changhua Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.5

Objective: This experiment was conducted to determine the differences in the apparent ileal (AID) and total tract digestibility (ATTD) of nutrients and indispensable amino acids (IAA) in high-fiber diets with wheat middlings, rice bran or alfalfa meal fed to Duroc${\times}$(Landrace${\times}$Yorkshire) (DLY) and Duroc${\times}$ (Berkshire${\times}$Jiaxing) (DBJ) growing barrows. Methods: Eighteen DLY and 18 DBJ growing barrows were randomly allotted to a $2{\times}3$ factorial arrangement involving 2 crossbreeds and 3 high-fiber diets. The experiment lasted 15 d with 10 d for diets adaptation, 3 d for feces collection and 2 d for digesta collection. Three diets were based on corn and soybean meal with 25% wheat middlings, rice bran and alfalfa meal respectively. Results: DBJ had a greater (p<0.05) AID of isoleucine, leucine, lysine, phenylalanine and valine and a lower (p<0.05) AID of methionine than DLY. The hindgut disappearance of acid detergent fiber for DBJ was greater (p<0.05) than DLY. The ATTD of gross energy, dry matter, organic matter, neutral detergent fiber and acid detergent fiber in wheat middlings diet were greater (p<0.05) than in rice bran and alfalfa meal diets. The hindgut disappearance of neutral detergent fiber and acid detergent fiber in wheat middlings diet or rice bran diet were the highest or lowest (p<0.05), and those of alfalfa meal diet were the middle. Barrows fed rice bran diet had a greater (p<0.05) hindgut disappearance of gross energy, dry matter and organic matter and lower hindgut disappearance of neutral detergent fiber and acid detergent fiber than barrows fed alfalfa meal diet Conclusion: DBJ growing barrows showed a significant higher digestibility of fiber in the hindgut and most IAA in the small intestine compared with DLY barrows. The digestibilities of chemical constituents and IAA were affected by the diets formulated with different fiber sources.

Core–shell structure g-MnO2-PANI carbon fiber paper-based flexible electrode material for high-performance supercapacitors

Xuxia Ling,Guoliang Zhang,Zhu Long,Xuefeng Lu,Zhibin He,Jie Li,Yuheng Wang,Dan Zhang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-

Good conductivity is the key to ensuring high electrochemical performance, and the uniformity of theactive material directly affects the electrical conductivity. This paper proposes a novel modified stepwisein situ polymerization method to prepare composite materials, and then uses the K/S value method tocharacterize the uniformity of polyaniline distribution. Specifically, the study presents a papermakingwet-lay technique to prepare a carbonfiber paper substrate (CP) that is light-weight,flexible, andfoldable while also having low internal resistance and high porosity. The g-MnO2 is wrapped with PANI toform a core–shell structure, and a modified stepwise in situ polymerization method is adopted to depositthe core–shell structure uniformly on the CP substrate by physical adsorption. This process yields aflexible electrode material for supercapacitors, called g-MnO2-PANI-CP electrode. Results show thatthe g-MnO2-PANI-CP electrode has a high specific capacitance of 642.5 F g 1 at a current density of 1A g 1, and also has a high energy density of 114.2 Wh kg 1 at a power density of 798.6 W kg 1. In addition,the electrode retains 81.3% of the initial capacitance after 5000 cycles, demonstrating high cyclingperformance.