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      • KCI등재

        Functional Analysis of Wheat TaPaO1 Gene Conferring Pollen Sterility Under Low Temperature

        Changping Zhao,Guoliang Yuan,Yukun Wang,Shaohua Yuan,Peng Wang,Wenjing Duan,Jianfang Bai,Hui Sun,Na Wang,Fengting Zhang,Liping Zhang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.1

        Thermosensitive male sterility plays an important role in wheat fertility and production. As a key enzyme for chlorophyll degradation, pheophorbide a oxygenase (PaO) can suppress cell death in plants. We cloned the wheat gene TaPaO1 from the thermosensitive genetic male sterile (TGMS) line BS366; it encodes a typical PaO protein, containing a conserved Rieske [2Fe-2S] iron–sulphur motif, a mononuclear non-heme iron-binding motif, and a C-terminal CxxC motif. TaPaO1 was expressed in all tissues and was upregulated during the meiosis stage of BS366 anthers under low temperature. Subcellular localization of TaPaO1 specifically labelled the surrounding of chloroplasts. TaPaO1 regulated by RD29A promoter which responded to low temperature led to pollen sterility in transgenic tobacco. Expression analysis showed that TaPaO1 exhibited a higher level of expression in the anther than in other tissues in transgenic tobacco plants during low temperature treatment. We propose that the higher senescence-related activity of TaPaO1 may lead to the cell death of anthers, which happens at an early developmental stage under low temperature. These results provide new insights into the function of PaO during the early developmental stage of anthers. PaO is closely related to cell death regardless of whether it exhibits increased activity or inactive.

      • KCI등재

        Effects of SiC content on microstructure and properties of carbon/ceramic conductive composites

        Xiao Gao,Xin Zheng,Jie Gao,Juncheng Liu,Jiahai BAI,Guoliang Yuan 한양대학교 세라믹연구소 2016 Journal of Ceramic Processing Research Vol.17 No.12

        The effects of SiC content on the phase composition, microstructure, sintering performance, mechanical and electricalproperties of the carbon/ceramic conductive composites were investigated. The results show that when SiC content increasesfrom 1 wt% to 3 wt%, lamellar graphite structure can be seen in the microstructure of the composites, and the porosity andwater absorption increases gradually. With further increase of SiC content to 6 wt% and 9 wt%, lamellar graphite disappears,and granular SiC crystals fill in the ceramic phase, thus increasing the densification degree of the composites. When SiCcontent is in the range of 1 wt% to 3 wt%, the shrinkage rate decreases sharply while the weight loss decreases a little. As SiCcontent increases from 3 wt% to 9 wt%, both the shrinkage rate and weight loss basically keep unchanged. With the increaseof SiC content, the bending strength of the composites first decreases and then increases, and finally reaches the peak value at9 wt% SiC. However, the resistivity presents an opposite variation trend, and the conductivity is best as SiC content is 9 wt%.

      • KCI등재

        Genome-wide identification and functional prediction of silicon (Si) transporters in poplar (Populus trichocarpa)

        Hassan Md Mahmudul,Martin Samir,Feng Kai,Yates Timothy B.,Yuan Guoliang,Martin Madhavi Z.,Martin Stanton,Muchero Wellington,Griffiths Natalie A.,Weston David J.,Yang Xiaohan 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2

        Silicon (Si) enhances plant tolerance to various biotic and abiotic stressors such as salinity, drought, and heat. In addition, Si can be biomineralized within plants to form organic carbon-containing phytoliths that can have ecosystem-level consequences by contributing to long-term carbon sequestration. Si is taken up and transported in plants via different transporter proteins such as influx transporters (e.g., Lsi1, Lsi6) and efflux transporters (e.g., Lsi2). Additionally, the imported Si can be deposited in plant leaves via silicification process using the Siliplant 1 (e.g., Slp1) protein. Functional homologs of these proteins have been reported in different food crops. Here, we performed a genome-wide analysis to identify different Si transporters and Slp1 homologs in the bioenergy crop poplar (Populus trichocarpa Torr. and A. Gray ex W. Hook). We identified one channel-type Si influx transporter (PtLsi1; Potri.017G083300), one Si efflux transporter (PtLsi2; Potri.012G144000) and two proteins like Slp1 (PtSlp1a; Potri.004G168600 and PtSlp1b; Potri.009G129900) in the P. trichocarpa genome. We found a unique sequence (KPKPPVFKPPPVPI) in PtSlp1a which is repeated six times. Repeated presence of this sequence in PtSlp1a indicates that this protein might be important for silicification processes in P. trichocarpa. The mutation profiles of different Si transporters in a P. trichocarpa genome-wide association study population identified significant and impactful mutations in Potri.004G168600 and Potri.009G129900. Using a publically accessible database (http://bar.utoronto.ca/eplant_poplar/), digital expression analysis of the putative Si transporters in P. trichocarpa found low to moderate expression in the anticipated tissues, such as roots and leaves. Subcellular localization analysis found that PtLsi1/PtLsi2 are localized in the plasma membrane, whereas PtSlp1a/PtSlp1b are found in the extracellular spaces. Protein–Protein interaction analysis of PtLsi1/PtLsi2 identified Delta-1-pyrroline-5-carboxylate synthase (P5CS) as one of the main interacting partners of PtLsi2, which plays a key role in proline biosynthesis. Proline is a well-known participant in biotic and abiotic stress tolerance in plants. These findings will reinforce future efforts to modify Si accumulation for enhancing plant stress tolerance and carbon sequestration in poplar.

      • KCI등재

        Design of carbonized unidirectional polyimide aerogel for CO2 capture: Effect of pore morphology/topology on CO2 capture

        Xinfu Zhao,Yihao Nie,Xibin Yi,Shimo Yu,Jing Zhang,Xiaochan Liu,Zhipeng Yuan,Sijia Liu,Jian Zhang,Guoliang Dou,Mengdi Wang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

        Design of efficient CO2 adsorbent is an effective way to capture CO2. Bamboo-derived activated carbon isa promising CO2 adsorbent. Inspired by this, carbonized unidirectional polyimide (CUPI) aerogels werenewly designed by the ice templating method using directional freezing technology. The pore propertyof CUPI aerogel can be tuned by changing the solidification velocity, and a series of CUPI aerogels weredesigned to explore the CO2 capture performance in view of pore morphology/topology. As a result,the obtained CUPI-8 exhibits higher CO2 adsorption capacity compared to carbonized polyimide aerogelsdue to the synergistic effect of micropores and lamellar pores. Specially, the CO2 adsorption capacity overCUPI-8 can be up to 5.75 mmol/g at 20 bar and 298 K. Furthermore, the adsorption isotherm of CUPI-8still shows an upward trend even nearly 20 bar. The isosteric adsorption heat (Qst) value of CUPI-8 isnearly in the ideal scope of 30–50 kJ mol1. The efficient CO2 adsorption performance and good chemicalstability of CUPI aerogel makes it a promising absorbent for practical application. This work provides anew opportunity for researching the effect of pore morphology/topology and the synergistic effect ofhierarchical pores on CO2 capture.

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