Melatonin Attenuates Noise Stress-induced Gastrointestinal Motility Disorder and Gastric Stress Ulcer: Role of Gastrointestinal Hormones and Oxidative Stress in Rats

( Lei Zhang ),( Ji T Gong ),( Hu Q Zhang ),( Quan H Song ),( Guang H Xu ),( Lei Cai ),( Xiao D Tang ),( Hai F Zhang ),( Fang E Liu ),( Zhan S Jia ),( Hong W Zhang ) 대한소화기기능성질환·운동학회 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.2

Background/Aims There are increasing evidences for gastrointestinal motility disorder (GIMD) and gastric stress ulcer induced by noise stress. The present study was to investigate the reversed effect of melatonin on GIMD and gastric stress ulcer induced by noise stress and potential mechanism. Methods Noise stress was induced on rats, and melatonin (15 mg/kg) was administered to rats by intraperitoneal injection. Differences were assessed in gastric residual rate (GRR), small intestine propulsion rate (SPR), Guth injury score, cortisol, gastrointestinal hormones (calcitonin-gene-related peptide and motilin) and oxidative stress markers (superoxide dismutase and malondialde hyde) in blood plasma as well as gastric mucosa homogenate with or without melatonin. The pathological examination of gastric mucosa was also performed. Results The GRR and SPR were improved by noise stress compared with control (P < 0.05). The pathological examination and Guth injury score revealed gastric stress ulcer. Moreover, the levels of cortisol, motilin and malondialdehyde in blood plasma and malondialdehyde in gastric mucosa homogenate were increased by noise stress (P < 0.05). CGRP and superoxide dismutase activity in both of blood plasma and gastric mucosa homogenate were significantly decreased (P< 0.05). Furthermore, melatonin reversed changes in GRR, SPR, pathological examination, Guth injury score, cortisol, motilin, CGRP, superoxide dismutase activity and malondialdehyde (P < 0.05). Conclusions Melatonin is effective in reversing the GIMD and gastric stress ulcer induced by noise stress. The underlying mechanism may be involved in oxidative stress and gastrointestinal hormones. (J Neurogastroenterol Motil 2015;21:189-199)

MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells

Zhang, Lei-Lei,Wu, Jiang,Liu, Qiang,Zhang, Yan,Sun, Zhu-Lei,Jing, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

Association between Polymorphisms of MSTN and MYF5 Genes and Growth Traits in Three Chinese Cattle Breeds

Zhang, R.F.,Chen, H.,Lei, C.Z.,Zhang, C.L.,Lan, X.Y.,Zhang, Y.D.,Zhang, H.J.,Bao, B.,Niu, H.,Wang, X.Z. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.12

The objective of this study was to assess the association of polymorphisms in MSTN and MYF5 genes with growth traits in three Chinese cattle breeds. Only one homozygous animal with BB genotype at MSTN locus was observed in Jiaxian population which was at Hardy-Weinberg disequilibrium (p<0.05). The frequencies of allele A at MSTN locus and allele B at MYF5 locus in the three Chinese breeds were 0.9550/0.9730/0.9720 and 0.8275/0.7581/0.7523, respectively. Allele A at MSTN locus and allele B at MYF5 locus were dominant in these three populations. No statistically significant differences in growth traits were observed between the genotypes of the Jiaxian breed at MSTN and MYF5 loci and the Nanyang breed at MYF5 locus. However, there were statistically significant differences between the genotypes at MSTN locus of the Nanyang breed for WH, HG, HGI and HGBLR (p<0.05), and of the Qinchuan breed for BLI (p<0.05). The SNP in MYF5 had significant effects on WH and HHC of Qinchuan animals (p<0.05). These results suggest that MSTN and MYF5 are strong candidate genes that influence growth traits in cattle. Other SNPs of MSTN and MYF5 or other linked genes should also be studied, which could lead to the development of selection plans to improve the performance of Chinese cattle and also promote the breeding of genuine beef cattle in China.

Anomalous magnetization jumps in granular Pb superconducting films

Zhang An-Lei,Jiang Wan-Yan,Chen Xing-Hong,Zhang Xiao-Ke,Lu Wen-Lai,Chen Fei,Feng Zhen-Jie,Cao Shi-Xun,Zhang Jin-Cang,Ge Jun-Yi 한국물리학회 2022 Current Applied Physics Vol.35 No.-

In granular superconductors, the grain boundaries are closely related to the vortex dynamics and the macroscopic superconducting properties. In our research, Pb films with different grain sizes were prepared by tuning the substrate temperature. With the grain size decreasing, Pb films are prone to feature the anomalous magnetization jumps in the M − T curves, while in the M − H curves flux avalanche happens. Both phenomena appear in the same region of the H − T phase diagram and thus are considered to have the same origin. The further theoretical analysis shows that with grain size decreasing the pinning mechanism evolves from a mixed δTc and δl pinning to the δl pinning mechanism. The results shed light on the study of pinning mechanism for granular superconductors and is beneficial to the potential application of manipulating vortex pinning by regulation of intrinsic defects.

Chinese Society of Allergy Guidelines for Diagnosis and Treatment of Allergic Rhinitis

Lei Cheng,Jianjun Chen,Qingling Fu,Shaoheng He,Huabin Li,Zheng Liu,Guolin Tan,Zezhang Tao,Dehui Wang,Weiping Wen,Rui Xu,Yu Xu,Qintai Yang,Chonghua Zhang,Gehua Zhang,Ruxin Zhang,Yuan Zhang,Bing Zhou,Do 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4

Allergic rhinitis (AR) is a global health problem that causes major illnesses and disabilities worldwide. Epidemiologic studies have demonstrated that the prevalence of AR has increased progressively over the last few decades in more developed countries and currently affects up to 40% of the population worldwide. Likewise, a rising trend of AR has also been observed over the last 2-3 decades in developing countries including China, with the prevalence of AR varying widely in these countries. A survey of self-reported AR over a 6-year period in the general Chinese adult population reported that the standardized prevalence of adult AR increased from 11.1% in 2005 to 17.6% in 2011. An increasing number of original articles and imporclinical trials on the epidemiology, pathophysiologic mechanisms, diagnosis, management and comorbidities of AR in Chinese subjects have been published in international peer-reviewed journals over the past 2 decades, and substantially added to our understanding of this disease as a global problem. Although guidelines for the diagnosis and treatment of AR in Chinese subjects have also been published, they have not been translated into English and therefore not generally accessible for reference to non-Chinese speaking international medical communities. Moreover, methods for the diagnosis and treatment of AR in China have not been standardized entirely and some patients are still treated according to regional preferences. Thus, the present guidelines have been developed by the Chinese Society of Allergy to be accessible to both national and international medical communities involved in the management of AR patients. These guidelines have been prepared in line with existing international guidelines to provide evidence-based recommendations for the diagnosis and management of AR in China.

Preparation and Characterization of a Novel Hyperbranched Poly(aryl ether ketone) Terminated with Cobalt Phthalocyanine to be Used for Oxidative Decomposition of 2,4,6-Trichlorophenol

Lei Lei,Yunhe Zhang,Xiujie Li,Jianxin Mu,Guibin Wang,Zhenhua Jiang,Shuling Zhang 한국고분자학회 2010 Macromolecular Research Vol.18 No.4

A novel hyperbranched poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-HPAEK)was prepared using 1,3,5-tris[4-(4-fluorobenzoyl) phenoxy] benzene (B3), as a ‘core’ molecule, 4,4'-(hexafluoroisopropylidene)diphenol (6F-BPA) as an A2 monomer, and cobalt phthalocyanine as an end-capping reagent. CoPc-THPAEK showed good solubility, thermal stability and catalytic activity in the oxidative decomposition of 2,4,6-trichlorophenol (TCP) under H2O2 or KHSO5. Moreover, the oxidative effect of KHSO5 was superior to that of H2O2,and the catalytic activity of CoPc-T-HPAEK was much higher than that of linear poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-LPAEK) and linear poly(aryl ether ketone) with a cobalt phthalocyanine pendant unit (CoPc-S-LPAEK).

A Multi-objective Two-sided Disassembly Line Balancing Optimization Based on Artificial Bee Colony Algorithm: A Case Study of an Automotive Engine

Lei Zhang,Yuanfeng Wu,Xikun Zhao,Shiwen Pan,Ziqi Li,Hong Bao,Yongtin Tian 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.5

Disassembly is an important part of green manufacturing and remanufacturing. The disassembly line is an optimum form for mass and automatic disassembly in the industry. To optimize the disassembly system and the use of resources, the disassembly line balancing problem (DLBP) has attracted much attention. Compared with the conventional one-sided straight disassembly line, the two-sided disassembly line can use both the left and right sides of a conveyor belt for disassembly operation, thereby improving production efficiency, especially for large-sized and complicated products. On the other hand, due to the constraints and precedence among parts, it is a challenge to plan the disassembly scheme for a two-sided disassembly line. In this paper, a model is established to solve a two-sided disassembly line balancing problem (TDLBP). First, a hybrid graph is utilized to express constraints and precedence relationships, and a novel encoding and decoding method is developed for the disassembly scheme planning of a two-sided line for handling the challenge caused by constraints and precedence among parts. Then, a multi-objective TDLBP optimization model is proposed including the number of matedworkstations, idle time, smoothness index, the auxiliary indicator, and a meta-heuristic based on an artificial bee colony (ABC) algorithm is designed to solve TDLBP. Finally, the proposed model and method are applied to an automotive engine case, and the results are compared with NSGA-II, hybrid artificial bee colony algorithm (HABC), and multi-objective flower pollination algorithm (MOFPA) to verify the practicality of the proposed model in solving the TDLBP.

Synthesis and gas sensing properties of high porosity n-type nickel ferrite thin film assisted by altering magnetic field

Lei Zhang,Wanli Jiao 한국물리학회 2015 Current Applied Physics Vol.15 No.7

NiFe2O4 thin film with high porosity based gas sensors had been prepared and their microstructure and gas sensing property were investigated. The sensing layer, consisted of perpendicular overlapped NiFe2O4 chains which were induced by altering magnetic field to self-assemble, had high porosity. The phase character and porous microstructure were characterized by X-ray diffraction (XRD) and a polarizing optical microscopy. The gas sensing tests results indicated that the sensor presented a high sensitivity to NH3 at 150 ℃, and was selective to NH3 below 200 ℃. The large porosity microstructure should benefit the reaction between target gas and sensing material and the detection of low concentration gas at low working temperature. In repeatability tests, the response and recovery time values had only narrow fluctuations.

Multi-domain Security Authentication Protocol for Hybrid Cloud

Zhang Qikun,Zhang Lei,Gan Yong,Duan zhaolei,Zheng Jun 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.8

In recent years, with the high-speed development of cloud computing and its universal application, the cloud security technology is more and more important. In cloud computing, it is mainly through the resource sharing and collaborative action to meet the demand for an unlimited access speed, unlimited storage space and a reliable resource protection for users. For the secure access resources among different domains in cloud network, today most Cloud Computing Systems provide data security and mutual authentication with asymmetric and traditional public key cryptography. For these researches, the authentication process is cumbersome, and the certificate management is complex, which would bring some inconvenience for mutual authentication among servers or users in a Hybrid Cloud. This paper provides a security authentication protocol among multiple domains. It adopts the direct product decomposition and linear mapping technology of cyclic group to achieve mutual authentication between members in multi-domain systems or heterogeneous networks. Extensive security and performance analysis show that the proposed schemes have the advantages of in security, computation consumption and Communication consumption. It is suitable for safety authentication in large-scale Cloud computing environment.

Chirality detection of amino acid enantiomers by organic electrochemical transistor

Zhang, Lijun,Wang, Guiheng,Xiong, Can,Zheng, Lei,He, Jianbo,Ding, Yunsheng,Lu, Hongbo,Zhang, Guobing,Cho, Kilwon,Qiu, Longzhen Elsevier 2018 Biosensors & bioelectronics Vol.105 No.-

<P><B>Abstract</B></P> <P>Chiral recognition of α-amino acids is attracting increasing interest due to the importance of α-amino acids in protein metabolism as well as in food products and pharmaceuticals. Organic electrochemical transistors (OECTs) with gate electrodes modified with molecularly imprinted polymer (MIP) films were fabricated and successfully used as highly selective and sensitive chiral recognition biosensors for <SMALL>D/L</SMALL>-tryptophan (<SMALL>D</SMALL> <SMALL>/</SMALL> <SMALL>L</SMALL>-Trp) and <SMALL>D/L</SMALL>-tyrosine (<SMALL>D</SMALL> <SMALL>/</SMALL> <SMALL>L</SMALL>-Tyr). The MIP films, which can specifically recognize and has an electrocatalytic effect on the oxidation of Trp and Tyr, together with the amplification function of an OECT, provide a highly sensitive and selective OECT biosensor. The sensor showed a linear response range for <SMALL>L</SMALL>-Trp and <SMALL>L</SMALL>-Tyr from 300 nM to 10 μM with a sensitivity of 3.19 and 3.64 μA/μM, respectivity. And the detection limit for <SMALL>L</SMALL>-Trp and <SMALL>L</SMALL>-Tyr is of 2 nM and 30 nM (S/N > 3). The selectivity factors of <SMALL>L</SMALL>-Trp, <SMALL>D</SMALL>-Trp, <SMALL>L</SMALL>-Tyr and <SMALL>D</SMALL>-Tyr to their enantiomers are 11.6, 3.5, 14.5 and 2.6, respectively. This method can pave the way for widespread applications of OECT-based sensors in chiral material identification.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An novel chiral recognition MIP-OECT sensor was developed. </LI> <LI> The MIP films showed good performance of selective and electro-catalytic oxidation for Trp and Tyr. </LI> <LI> The MIP films together with the amplification function of an OECT afforded a highly sensitive, selective sensor for chirality detection of amino acid enantiomers. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>