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        Functional Analysis of Wheat TaPaO1 Gene Conferring Pollen Sterility Under Low Temperature

        Changping Zhao,Guoliang Yuan,Yukun Wang,Shaohua Yuan,Peng Wang,Wenjing Duan,Jianfang Bai,Hui Sun,Na Wang,Fengting Zhang,Liping Zhang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.1

        Thermosensitive male sterility plays an important role in wheat fertility and production. As a key enzyme for chlorophyll degradation, pheophorbide a oxygenase (PaO) can suppress cell death in plants. We cloned the wheat gene TaPaO1 from the thermosensitive genetic male sterile (TGMS) line BS366; it encodes a typical PaO protein, containing a conserved Rieske [2Fe-2S] iron–sulphur motif, a mononuclear non-heme iron-binding motif, and a C-terminal CxxC motif. TaPaO1 was expressed in all tissues and was upregulated during the meiosis stage of BS366 anthers under low temperature. Subcellular localization of TaPaO1 specifically labelled the surrounding of chloroplasts. TaPaO1 regulated by RD29A promoter which responded to low temperature led to pollen sterility in transgenic tobacco. Expression analysis showed that TaPaO1 exhibited a higher level of expression in the anther than in other tissues in transgenic tobacco plants during low temperature treatment. We propose that the higher senescence-related activity of TaPaO1 may lead to the cell death of anthers, which happens at an early developmental stage under low temperature. These results provide new insights into the function of PaO during the early developmental stage of anthers. PaO is closely related to cell death regardless of whether it exhibits increased activity or inactive.

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        Effect of Temperature and Strain Rate on Deformation Mode and Crack Behavior of 7B52 Laminated Aluminum Alloy Under Impact Loading

        Wenhui Liu,Ping Cao,Chenbing Zhao,Yufeng Song,Changping Tang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.11

        The dynamic mechanical behaviors of 7B52 laminated aluminum alloy under different impact deformation conditions wereinvestigated using split Hopkinson pressure bar, optical microscopy, electron backscatter diffraction and transmission electronmicroscopy. The results show that the 7B52 laminated aluminum alloy displays positive strain rate sensitivity and negativetemperature sensitivity. Cracks are formed firstly at the interface between the 7A62 hard layer and the 7A01 middle layerunder a low strain rate of 2200 s−1. When the strain rate increases to 3500 s−1, the deformation shear band is firstly formed inthe 7A52 soft layer. The deformation shear band transformed to the transition shear band with the increase of strain rate, andthen crack initiated. Adiabatic shear bands and cracks are formed in both of the hard and soft layers when samples impactedat a high strain rate (5500 s−1). Most of the cracks and adiabatic shear bands are unable to penetrate the middle layer dueto the good toughness of the 7A01 alloy. The deformation of each layer of 7B52 laminated aluminum alloy becomes moreuniform when the temperature increases, which was attributed to the reduction of difference in strength among the threelayers and the coordinating effect of grain boundary on deformation.

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        Responses of Antioxidant Enzyme Activity to Different Fertilizer and Soil Moisture Conditions in Relation to Cold Resistance in Zanthoxylum armatum

        Jiangtao Fan,Jingyan Wang,Xianzhi Liu,Changping Zhao,Chaobing Zhou,Tasheen Saba,Jiaojiao Wu,Wenkai Hui,Wei Gong 한국원예학회 2022 원예과학기술지 Vol.40 No.3

        Zanthoxylum armatum, known as green prickly ash, is a major condiment species planted on a large scale in southwest China. However, its limited cold tolerance has been a significant disadvantage for its cultivation in low-temperature regions. To better understand the antioxidant enzyme activities related to cold resistance, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities as well as malondialdehyde (MDA) content were investigated under 16 fertilizer and soil moisture treatments to explore the cold resistance response mechanism in Z. armatum. The results showed that low temperatures could significantly affect antioxidant enzyme activities in the leaves of Z. armatum seedlings. The antioxidant enzyme activities of seedlings treated with soil moisture and fertilizer treatments were significantly higher than those without fertilizer. Furthermore, the comprehensive evaluation index (CEI) was positively correlated with SOD, POD, and CAT activities but negatively correlated with MDA content. Meanwhile, the treatment T12 (60% field water capacity + 300 kg·ha-1 nitrogen + 30 kg·ha-1 phosphoric anhydride) had the highest CEI value, whereas T1 (20% water capacity) had the lowest. Based on the regression equation for CEI improvement, the predicted optimal levels for field water capacity (FWC), nitrogen, phosphorus, and potassium were 59.6%–63.4% FWC (average 61.5%), 114.2–248.8 kg N/ha (average 181.5 kg N/ha), 40.1–108.5 kg P2O5/ha (average 74.3 kg P2O5/ha), and 200.2–254.4 kg K2O/ha (average 227.3 kg K2O/ha), respectively. The present study provides the optimal soil moisture and fertilizer concentration required to enhance protective enzyme activity and low-temperature tolerance, which is crucial for improving cold resistance in Z. armatum.

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