Characteristics and Regulating Role in Thermotolerance of the Heat Shock Transcription Factor ZmHsf12 from Zea mays L.

Guoliang Li,Yuanyuan Zhang,Huaning Zhang,Yujie Zhang,Lina Zhao,Zihui Liu,Xiulin Guo 한국식물학회 2019 Journal of Plant Biology Vol.62 No.5

Plant heat shock transcription factors (Hsfs) areimportant regulators of heat shock signal transduction pathway. There are 30 members of the Hsf family in maize, only twoof which, ZmHsf06 and ZmHsf12, belong to subclass A1. Our previous work demonstrated that ZmHsf06-overexpressingArabidopsis lines showed improved tolerance to heat anddrought stresses. In this study, we isolated ZmHsf12 fromyoung leaves of maize (Zea mays L.) using homologouscloning methods. The CDS (coding sequence) of ZmHsf12 is1,494 bp and encodes a putative protein consisting of 497amino acids which possesses domains such as DBD (DNAbindingdomain), OD (oligomerization domain), NLS (nuclearlocalization signal), NES (nuclear export signal), and anAHA (activator) motif. The ZmHsf12-GFP fusion protein islocalized to the cell nucleus. ZmHsf12 was expressed inmany maize organs, and its expression was up-regulated byheat shock. Furthermore, we characterized the function ofZmHsf12 in yeast and Arabidopsis. Yeast cells overexpressingZmHsf12 showed enhanced heat tolerance. ZmHsf12-overexpressing Arabidopsis seedlings displayed significantincreases in both basal and acquired thermotolerance. Comparedto WT seedlings, the ZmHsf12-overexpressing lines displayedboth increased chlorophyll contents and higher survivalrates. Also, the expression of AtHsps was increased higher inthe ZmHsf12-overexpressing Arabidopsis lines after heatstress. The results of our study strongly suggested thatZmHsf12 may take part in plant response to heat stress.

Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa

Guoliang Li,Shifan Zhang,Fei Li,Hui Zhang,Shujiang Zhang,Jianjun Zhao,Rifei Sun 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.1

Plants protect against viruses through passive and ac- tive resistance mechanisms, and in most cases charac- terized thus far, natural recessive resistance to potyvi- ruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimo- lecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and trans- lation initiation factors to reveal the working of key amino acids.

Experimental Comparison of Bernoulli Gripper and Vortex Gripper

Xin Li,Ning Li,Guoliang Tao,Hao Liu,Toshiharu Kagawa 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

A Bernoulli gripper and a vortex gripper are types of pneumatic grippers that have similar functions but different working principles. They are widely used for performing gripping, transferring, and placing operations in automated production lines. This study systematically compares the two grippers and provides an objective reference to enable users to choose an appropriate gripper. First, we present the working principles of both grippers. Then, we measure the curves of suction force, air consumption, and upstream pressure; furthermore, we analyze and compare the energy consumption from the viewpoint of the entire pneumatic system. The results reveal the relationships with the air consumption and total energy consumption. Next, we measure the pressure distribution formed on the upper surface of the workpiece. From the experimental results, the principles of the two grippers are stated and compared. Furthermore, we use the pressure distribution data to estimate the deformation and inner stress of the workpiece. The influence of the grippers on the workpiece are analyzed and compared from a material mechanics viewpoint. Finally, we use three types of rough surfaces to qualitatively evaluate and compare the performance of the grippers when gripping a workpiece with a rough surface

Experimental Study on Dynamic Tensile Properties of Macro-Polypropylene Fiber Reinforced Cementitious Composites

Guoliang Yang,Jingjiu Bi,Zhiwen Dong,Ying Li,Yi Liu 한국콘크리트학회 2023 International Journal of Concrete Structures and M Vol.17 No.1

Using a high-speed photography system and a split Hopkinson pressure bar, macro-polypropylene fiber reinforced cementitious composites are tested to reveal the effects of the macro-polypropylene fiber volume fraction and loading rate on the dynamic tensile strength and failure mode. We also analyze the functional relationship between the dynamic tensile strength, loading rate, and fiber volume fraction, and study the splitting failure process using digital image correlation technology. The evolution law of the strain and displacement fields of the specimens is obtained, and the effect of the fiber volume fraction on the crack initiation strain value is quantitatively studied. The results show that the appropriate fiber content (1.5–2%) can significantly improve the dynamic tensile strength, while a higher fiber content (2.5%) leads to deterioration of the specimen. Adding macro-polypropylene fiber prevents the specimen from undergoing central tensile fracturing under dynamic loading, and distributes the impact load more evenly, thus improving the ability of the specimen to resist cracking.

Geometric optimization and performance analysis of radial MR valve using Taguchi orthogonal experiment method

Guoliang Hu,Feng Zhou,Wencai Zhu,Lifan Yu,Gang Li 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.9

Aiming at the problems of increasing external dimensions and deteriorating key performance indicators in the design process of magnetorheological (MR) valve by using structural optimization method, a geometric optimization design methodology for the optimal design of a MR valve structure under specific volume constraints is proposed in this article. The optimization methodology couples the finite element model (FEM) constructed in COMSOL software with the Taguchi orthogonal experiment and response surface technology to build an approximate response surface function for the identified independent variables. Suitable optimization algorithms are then utilized to determine the optimal geometry of the MR valve, thereby maximizing the valve performance. Firstly, a radial MR valve with a single excitation coil was presented, and its structure and working principle were also elaborated. A mathematical model of the pressure drop was derived on the basis of the Bingham-Papanastasiou non-Newtonian constitutive model of MR fluid and the magnetic circuit had been analyzed with the FE analysis methodology. Then, a second-order response surface model (RSM) had been fitted for the magnetic flux density in the radial flow channel and spool region of the radial MR valve based on the Taguchi orthogonal experimental design. The fitted model was a function of the four independent variables of the radial MR valve, and the accuracy of the developed response surface function over the entire design space had also been estimated. Meanwhile, predictions made by the RSM and FE models were evaluated by analysis of variance and it was exhibited that the RSM model’s results agree with FE result fairly. Subsequently, the geometric optimization problem had been formulated for the constructed RSM exploiting the genetic algorithm to find the global optimum geometrical parameters of the radial MR valve. Furthermore, the experimental test rig was setup to explore the pressure drop and the response time characteristics of the initial and optimal radial MR valve as well as the dynamic performance of the MR valve controlled cylinder system under different excitation conditions. The experimental results show that under the applied current of 2 A, the pressure drop and adjustable coefficient of the optimal radial MR valve observably increased with values of 3.15 MPa and 5.40, respectively, when compared to 2.11 MPa and 4.22 of their respective initial values. Also, at the applied current of 1.25 A, the damping force of the MR valve controlled cylinder system enlarged by 46 %, with its optimal value being 3.65 kN and initial value as 2.50 kN, which was an excellent verification of the correctness of the RSM and the effectiveness of the optimal design.

Interval Estimation for Discrete Sequential Systems Under Round-robin Protocol

Xin Li,Guoliang Wei,Derui Ding 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.1

In this paper, an interval estimator is designed for discrete sequence systems (DSSs) with delayed measurements. In order to reduce network transmission burden and prevent data collision, the round-robin (RR) protocolis employed to schedule the transmission order of the nodes. The purpose of the problem addressed is to design anovel interval estimator consisting of two coupled sub-estimators for DSSs with the bounded noises, such that 1) theerror dynamics of the estimation upper bound and estimation lower bound are asymptotically stable, 2) the effectfrom the bounded noises on the estimation accuracy is attenuated at a given level by means of an H∞ norm. Basedon the positive system theory combined with the Lyapunov stability theory, the real state is ensured in the interval,whose bounded is determined by the estimation upper bound and estimation lower bound. Moreover, the desiredgains are obtained by solving a class of linear matrix inequalities. Finally, the effectiveness of the proposed intervalestimator is verified by two numerical examples.

Transplanting neurofibromatosis-1 gene knockout neural stem cells improve functional recovery in rats with spinal cord injury by enhancing the mTORC2 pathway

Chen Guoliang,Li Xianlong,Zhu Hongzhang,Wu Huachuan,He Dacheng,Shi Liangyu,Wei Fuxin,Liu Xizhe,Chen Ningning,Liu Shaoyu 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

The poor survival and low efficiency of neuronal differentiation limits the therapeutic effects of transplanted neural stem cells in the treatment of spinal cord injury. Neurofibromatosis-1 (NF-1) is a tumor suppressor gene that restricts the rapid and abnormal growth and differentiation of neural cells. In the present study, lentiviral vectors were used to knock out NF-1, Ricotr (the core member of mTORC2) or NF-1+Ricotr in neural stem cells in vitro, and the NF-1, Ricotr or NF-1+Ricotr knockout neural stem cells were transplanted at the lesion site in a rat model of spinal cord injury (SCI). We first demonstrated that targeted knockout of NF-1 had an antiapoptotic effect and improved neuronal differentiation by enhancing the mTORC2/Rictor pathway of neural stem cells in vitro. Subsequently, transplanting NF-1 knockout neural stem cells into the injured site sufficiently promoted the tissue repair and functional recovery of rats with spinal cord injury by enhancing the survival and neuronal differentiation of grafted neural stem cells. Collectively, these findings reveal a prominent role of NF-1 in neural stem cell biology, which is an invaluable step forward in enhancing the benefit of neural stem cell-mediated regenerative cell therapy for spinal cord injury and identifies the transplantation of NF-1 knockout neural stem cells as a promising strategy for spinal cord injury.

Intensity of Intraoperative Spinal Cord Hyperechogenicity as a Novel Potential Predictive Indicator of Neurological Recovery for Degenerative Cervical Myelopathy

Chen Guoliang,Wei Fuxin,Li Jiachun,Shi Liangyu,Zhang Wei,Wang Xianxiang,Xu Zuofeng,Liu Xizhe,Zou Xuenong,Liu Shaoyu 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.7

Objective: To analyze the correlations between intraoperative ultrasound and MRI metrics of the spinal cord in degenerative cervical myelopathy and identify novel potential predictive ultrasonic indicators of neurological recovery for degenerative cervical myelopathy. Materials and Methods: Twenty-two patients who underwent French-door laminoplasty for multilevel degenerative cervical myelopathy were followed up for 12 months. The Japanese Orthopedic Association (JOA) scores were assessed preoperatively and 12 months postoperatively. Maximum spinal cord compression and compression rates were measured and calculated using both intraoperative ultrasound imaging and preoperative T2-weight (T2W) MRI. Signal change rates of the spinal cord on preoperative T2W MRI and gray value ratios of dorsal and ventral spinal cord hyperechogenicity on intraoperative ultrasound imaging were measured and calculated. Correlations between intraoperative ultrasound metrics, MRI metrics, and the recovery rate JOA scores were analyzed using Spearman correlation analysis. Results: The postoperative JOA scores improved significantly, with a mean recovery rate of 65.0 ± 20.3% (p < 0.001). No significant correlations were found between the operative ultrasound metrics and MRI metrics. The gray value ratios of the spinal cord hyperechogenicity was negatively correlated with the recovery rate of JOA scores (ρ = -0.638, p = 0.001), while the ventral and dorsal gray value ratios of spinal cord hyperechogenicity were negatively correlated with the recovery rate of JOA-motor scores (ρ = -0.582, p = 0.004) and JOA-sensory scores (ρ = -0.452, p = 0.035), respectively. The dorsal gray value ratio was significantly higher than the ventral gray value ratio (p < 0.001), while the recovery rate of JOA-motor scores was better than that of JOA-sensory scores at 12 months post-surgery (p = 0.028). Conclusion: For degenerative cervical myelopathy, the correlations between intraoperative ultrasound and preoperative T2W MRI metrics were not significant. Gray value ratios of the spinal cord hyperechogenicity and dorsal and ventral spinal cord hyperechogenicity were significantly correlated with neurological recovery at 12 months postoperatively.

Game Analysis on External Purchase of Technology Innovation under Complete Information

Zhang Guoliang,Li Fengxiang 한국멀티미디어학회 2010 한국멀티미디어학회 국제학술대회 Vol.2010 No.-

In fierce market competition, technological innovation becomes a crucial element which effects the sustainable development of enterprises. Nowadays external purchase of innovative technology is important and is essentially the results of the game between enterprise and innovative research institutions. The two players in the game analyze counterpart's policy to choose the best strategy to achieve the greatest benefits.

Parametric analysis on diagonal bracing joints connected with self-tapping screw

Zhenhua Xu,Guoliang Bai,Biao Liu,Jiarui Li,Yifan Yang 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.41 No.1

In this paper, a three-dimensional finite element model (FEM) was established to study the performance of sliding resistance on the diagonal bracing joints of prefabricated steel frame (PSF) connected with self-tapping screw. In addition, the load-displacement curves of the joints were well verified by the test results, and a parametric study was conducted using the validated models to investigate the effect of steel grade, thickness of the tapped plate, the margin of bolt hole, the end and the central distance of bolt hole. The results showed that with the increase of steel grade, thickness of the tapped plate and the distance of the bolt holes, the anti-slide stiffness and the ultimate bearing capacity of the joint were significantly improved. It was recommended that steel grade should be Q345, Q390 or above. It was advisable to take the thickness of the tapped plate at 0.7d0 ~ 1.0d0, take the margin of bolt hole at 1.2d0 ~ 1.6d0, the central and the end distance of bolt hole should be greater than 2.0d0 and 1.2d0, respectively. The results could provide reference for engineering design.