한국해안으로부터 Purple, Non-Sulfur Photosynthetic Bacterium, Rhodobacter sp. EGH-24의 분리 및 특성

차미선,김기한,조순자,이나은,이정은,이재동,이상준,박재림 한국환경과학회 2003 한국환경과학회지 Vol.12 No.12

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28~32 ℃, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 % agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.

EDTA의 세정시간에 따른 근관 내 smear layer의 제거효과에 관한 연구

이자경,박상혁,최기운 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구는 EDTA 용액의 적용 시간에 다른 도말층의 제거 효과를 비교하여 EDTA 용액의 최적 적용 시간을 알고자 시행되었다. 단근치 35개를 NaOCI 용액 하에서 근관 형성 후 대조군은 NaOCI 용액 5㎖로 2분간, 실험군은 30초군, 1분군, 2분군으로 세분하여 17% EDTA 용액 5㎖로 각각 30초, 1분, 2분간 세정하였다. 세정이 끝난 치근의 도말층 제거 정도와 상아 세관의 erosion 정도를 주사전자현미경을 이용하여 관찰한 결과는 다음과 같다. 1. 대조군: 도말층이 전혀 제거되지 않았다. 2. 실험군: 1) 중간⅓: 세 군 모두에서 도말층이 거의 완전히 제거되었고, EDTA 적용 시간이 증가할수록 erosion 발생 빈도가 증가하였다. 2) 치근단⅓: 2분군이 다른 두 군에 비해 도말층 제거 정도가 우수하였다. 따라서 erosion 발생을 최소화면서 도말층을 효과적으로 제거하기 위해서는 17% EDTA 용액을 2분간 사용하는 것이 적절한 것으로 사료된다. This study was to verify that the combined application of NaOCI and EDTA was more effective in removal of smear layer than the application of NaOCI alone. Furthermore it was aimed to find out the optimal time for the application of EDTA. Thirty five single rooted teeth were cleaned and shaped. NaOCI solution was used as an irrigant during instrumentation. After instrumentation, root canals of the control group were irrigated with 5㎖ of NaOCI for 2 minutes. 30 sec, 1 min, and 2 min group were irrigated with 5㎖ of 17% EDTA for 30 sec, 1 min, and 2 min respectively. Then the roots were examined with scanning electron microscopy for evaluating removal of smear layer and erosion of dentinal tubule. The results were as follows; 1. The control group: The smear layer was not removed at all. 2. The other groups: 1) Middle⅓: All groups showed almost no smear layer. And the erosion occurred more frequently as increasing irrigation time. 2) Apical⅓: The cleaning effect of 2 min group was better than the others. The results suggest that 2 min application of 17% EDTA should be adequate to remove smear layer on both apical⅓ and middle⅓.

간호학생의 임상실습 교육을 위한 임상지도자 활용모델(Preceptorship Model) 적용 효과에 관한 연구

김조자,이원희,김소선,유해라,한신희,김기연,노영숙 대한간호협회 1997 대한간호 Vol.36 No.4

관동맥 질환 환자에서 전산화단층촬영상의 복부지방 분포 및 형광안저 촬영

한혜자,홍종면,차상훈,김동운,남기병,최미영,강세웅,박남규,이석재 충북대학교 의과대학 충북대학교 의학연구소 1996 忠北醫大學術誌 Vol.6 No.2

배경 및 목적 : 죽상경화증의 원인 인자중 하나인 복부지방의 축적도를 복부 전산화 단층 촬영술로 정량화 하여 관동맥 질환과의 상관 관계를 살펴보고자 하려고, 또한 형광 안저 촬영 검사상의 죽상 경화증 정도와 관동맥 질환과의 상관 관계를 알아보고자 하였다 대상 및 방법 : 관동맥 조영술을 시행한 환자중 관동맥조영술 소견상 50% 이상의 협착이 있는 경우를 관동맥 질환군(n=15)으로 정의하였고, 관동맥 조영술 소견상 관동맥 질환이 없고 허혈성 뇌혈관질환, 당뇨병 및 고혈압이 없는 환자들을 대조군(n=11)으로 하였다. 배꼽 위치에서 복부 전산화 단층 촬영을 시행하여 총 복부 지방면적(total abdomen fat area, TAFA). 복강내 지방면적(abdomen visceral fat area, AVFA) 및 복부 피하지방면적(abdomen subcutaneous fat area, ASFA)을 측정하였고, 그 비를 구하여 비교 분석하였다 또한 형광 안저 촬영 검사를 시행하여 동-정맥비와 동-정맥교차수 및 팔망막 순환기 시간을 측정하여 비교 분석하였다. 결과 : 1) 복부 전산화 단층 촬영 : 대조군과 관동맥 질환군은 복강내 지방면적/(AVFA/TAFA)은 각각 53.9±8.3, 56.1±8.5으로 통계적으로 유의한 차이가 없었고, 또 복강내 지방면적/복부 피하지방면적(AVFA/ASFA)도 각각 0.90±0.30, 0.82±0.26으로 통계적으로 유의한 차이가 없었다 (p>0.05). 2) 형광안저 촬영 검사 ; 대조군과 관동맥 질환군에서 동-전맥비(%)는 각각 72.1±4.9, 62.5±6.1, 동-정맥 교차수는 0.3±0.5, 1.1±1.1, 팔망막 순환기 시간(msec)은 15.3±1.8, 17.8±4.0으로 세 지표 모두에서 통계적으로 유의한 차이가 있었다 (p<0.05). 결론 : 관동맥 질환 환자에서 전산화 단층촬영을 이용하여 구한 복부지방의 분포양상(복강내 지방의 구성 비율 등)은 정상 대조군과 차이가 없었다. 관동맥 질환 환자와 정상 대조군에서 형광 안저 촬영 검사로 측정한 동정맥 직경비, 동정맥교차현상수 및 팔망막순환시간은 각각 통계적으로 의미 있는 차이가 있었다 Background : We quantitatively evaluated the abdominal fat distribution by computed tomography(CT) in coronary artery disease patients and the findings of fluorescence angiography in coronary artery disease patients. Methods: Among the patients who were done coronary angiography(CAG), the control group(n=11) has no angiographically significant stenosis(<50%) and the coronary artery disease(CAD) group(n=15) has more than one significant stenosis(>52%). We examined abdominal CT at umbilicus level and fluorescence angiography in all patients. In abdominal CT, we calculated total abdominal fat area(TAFA), abdominal visceral fat asea(AVFA) and abdominal subcutaneous fat area(ASFA) and their ratios. In fluorescence angiography, we calculated that artery-vein ratio(AV ratio), artery-vein cross number(AV cross No) and antecubital-retinal circulation time. Results: 1) Abdominal CT A. AVFA/TAFA(㎡) ; Control group : CAD group = 53.9±8.3 : 56.1±8.5 (p〉0.05) B. AVFA/ASFA(㎡) ; Control group : CAD group = 0.90±0.30 : 0.82±0.26 (p〉0.05) 2) Fluorescence angiography A. AV ratio(%) ; Control group : CAD group = 72.1±4.9 : 62.5±6.1 (p〈0.05) B. AV cross No ; Control group : CAD group = 0.3±0.5 : 1.1±1.1 (p〈0.05) C. Antecubital - retinal circulation time(ms) Control group : CAD group = 15.3±1.8 : 17.8±4.0 (p<0.05) Conclusion : There was significant association between the FAG findings and CAG findings but there was no significant association between the abdominal fat distribution by abdominal CT and CAG findings.

일 간호대학의 지역사회 방문간호사업 사례

강윤숙,최양자,고영애,전시자,조갑출,이옥철,주혜경,신기수,안은숙 적십자간호전문대학 2000 적십자간호전문대학논문집 Vol.22 No.-

중등학교의 성교육 개선방안을 위한 연구 : 경남지역 여학생을 중심으로 Gyeong Nam Area

박정희,구자옥,이남기 한국 가정과 교육 학회 1997 한국가정과교육학회지 Vol.9 No.2

The purpose of this study was to collect fundamental data for sex education by investigating the actual conditions of sex education, concerns about sex, knowledge of sex and consciousness about sex. The subjects were 212 middle school girls and 219 high school girls in Ggyeong Nam. The results were as follows 1.There was no significant difference between middle school girls and high school girls in knowledge of sex. 2.The most frequently experienced problem concering other sex was about dating and the many consultants were friends. 3.The stimulation of sexual desire was brought by filmㆍTVㆍVTR, andc advertisement, and sex information was acquired from friends or magazines in both schools students. 4.The reason of high school students for needing sex education was to solve moral problems and the reason of middle school students was to solve the anxiety in adolescence. 5.The students wanted their sex educator to be the expert in the field of sex education, home economist and nurse teacher, in order.

산업근로자의 식생활 의식행태 조사

김무룡,황연자,차경미,이순자,위광복,남철현,김기열,김홍길 慶山大學校 保健福祉硏究所 1997 保健福祉硏究 Vol.1 No.-

This study was carried out to grasp the awareness and habits of food intake and use the information for health education program in order to help healthy dietary life. The survey was done in cooperation with health manager in industries on 1,200 workers who was selected randomly from 5 types of industries from Sep. 2 to Oct. 31, 1995. The results are summarized as follows: 1) The percentage of subjects who assess themselves "Healthy" was more higher in female, in 20∼29 age group, in single, in daytime workers, in college graduate than other groups respectively. 2) As for the degree of health education satisfaction, 35.5% of the subjects answered "dissatisfaction" 3) As for the exercise, 52.7% exercised for their health and the rate of exercise was more higher in male, in 30 years age group, in single than other groups respectively. 4) The rate of smoking was 57.6% and the rate of drinking alcohol was 47.3%. 5) 92.8% of the subjects got stress everyday and the rate is more higher in female, in less than 19 years old, in single, in longer working duration, in lower income, in high school graduates, in workers of product field line than other groups respectively. 6) As for drinking times of coffee or soft drink, two times per day was 29.5%, more three times per day was 28.0%. As for the eating speed of meal, 46.0% of the subjects answered "fast" and the rate was more higher in male, in 30 years age group, in married group, than other groups respectively. 7) With regard to the level of preference to instant food, 23.1% preferred to take instant food. As for the level of preference to hot and salty food, 28.1% preferred to have hot and salty food. 8) The score of eating behavior was 3.38±32 point to 6 point full marks. 9) With regard to the correlation among related variables, the variables of gender, marital status and working duration had positive relation with the variables of regular meal intake, the preference to food intake between regular meals.

대학생의 노인복지에 대한 인식도

차병준,김무룡,김기열,차경미,김귀희,김현실,서인선,임상규,이순자,위광복,남철현 慶山大學校 保健福祉硏究所 1999 保健福祉硏究 Vol.2 No.-

The purpose of this study is to grasp the change of consciousness of young people in order to seek new approach of policy for aged era. 1,200 students attending collages and universities in three small or medium sized cities were inquired for 2 months, from October 1 to November 30, 1996. The result is as follows. 1. The characteristic of the respondents of this survey is that they include 72.1% of women, 40.4% of people aged between 20 to 21, and 49.1% of people who do not have any religion. Those who are from big cities and those who are form farming villages or fishing village occupy equally 40.2%, those who reside in that cities for a long period 49.0%, and those who live with their family 60.9%. 2. The family feature of the subject is that 31.5% of them are those whose father is the age of 55 and more than 55, 10% of them are those whose mothers age is so, 33.1% of them have 5 family members, 29.5% of them have the family income of 1,000,000 to 1,490,000 won, 83.9% of them are from middle class, 47.7% of them are the people whose grand parents have passed away, 70.4% of them live apart from their grand parents, and 60.9% of them are second child. 3. 64.0% of males, 70% of respondents who are aged over 24, 58.1% of respondents who are from rural communities, 62.4% of respondents who live in rural area, and 58.3% of respondents who live alone, prefer to live with grand parents, marked higher rate than other groups. The groups whose father and mother are the age of more than 55 marked higher rate of 55.9% and 58% each than any other groups(p<0.05). The groups whose family member is less than three, whose grand parents both have passed away in middle class, who live with their grand parents, and who are second children, give more positive answers than any other groups. 4. Those who are older, who are buddhists, who are from rural area or live in rural area, whose family is small, whose family income is small, who are from lower class and whose grand parents are still alive, shows preference to support their parents after marriage. 5. Females(89.4%), those who are from small or medium sized cities(89%), who live with their family(85.6%), whose father is the age of 55 and more than 55, whose mother is the age of 54 and less than 54, whose family member is 6, who are from middle class, whose grandparents are still alive and who are the eldest child in their family, give more response that they prefer to live apart from their children in their old age than any other groups. 6. What elderly people need most right now is past time(42.3%), and the respondents whose age is 20 to 21, who are buddhists, who are from middle sized cities and live alone, whose parents are the age of more than 55, who have small family income, who live with their grand parents and who are second children show higher level than any other groups. 7. 76.8% of respondents answer that they do not need their parent's fortune, marked still higher rate than the opposite answer. Those who are older, who are buddhists, who are from big cities, who reside in rural area, who live alone and have smaller family, who are in bad family economic conditions and who live with grand parents present higher rate of positive response. 8. 59.3% of respondents reply that they think about death from time to time, and those who are female, who are in the age of 20 to 21, who are christian, who live with their family, whose siblings or other family members offer their education expenses show higher rate than any other groups. 9. 92.3% of respondents answer that the budget for welfare facility for elderly people is very small. The younger the respondents are and the worse economic condition they have among those who live in big cities, the more answered that. 10. 50% of respondents think social security should provide for their old age, and 42.8% of them believe they themselves provide against it. The respondence that social security ought provide for it appeared more among those whose age are over 24, who are female, who reside in rural area, who are christian, who are in bad economic condition, and who ever used medical institutions in recent one month, marked higher level than other groups. 11. The adequate time of retirement is 59.97±5.60 for public officers, the highest and 59.28±6.35 for teachers, the next. 12. 35.2% of respondents reply that the adequate budget for the old age after their retirement at the age of 60 is 220 million won, showed the highest level of respondence. 13. The factors which affect on the old people in the charged welfare facilities are sex(p<0.01), the type of housing(p<0.05) and whole family income(p<0.05). 14. The factors which affect on remarriage are sex(p<0.05) and religion(p<0.05). For varying old population and expanded welfare service for them, government and relevant authority should give more attention to secure the budget, establish effective plans to expand various program and the facilities for elderly people in order that elderly people spend the rest of life time more happily and more fruitfully. To do that, each college and university should open Health courses, and educate and inform about health and welfare.

우리나라 논 관개용 지하수 수질 특성

김진호,조광래,임수정,이경자,경기천,엄미정,김희권,김찬용,이영한,이신찬,윤순강 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

This study was carried out to evaluate the status of the groundwater quality for paddy fields irrigation in Korea. Water samples were collected at 130 sites throughout the country. Samples were collected at three seasons-April, July and October-in 2000. According to our survey, the groundwater was found to be suitable for irrigation purpose. Average EC was shown 0.286 dS/m. Nitrate-nitrogen and Cl^(-) concentration was 5.6 ㎎/L, 32.95 ㎎/L which satisfied the Korean Standards for Irrigation Water. Nitrate-nitrogen concentration in each province was shown as following orders: Jeju (11.17 ㎎/L) > Chungnam (8.16 ㎎/L) > Gyeongbuk (6.64 ㎎/L) > Gyounggi (5.91 ㎎/L) > Chungnam (4.95 ㎎/L) > Gyeongnam (3.91 mgk) > Jeonbuk (3.50 ㎎/L) > Jeonnam (3.27 ㎎/L) > Gangwon (2.91 ㎎/L). The concentration by sampling seasons were October (6.62 ㎎/L) > July (5.88 mg&) > April (4.78㎎/L). As the soil of Jeju Province is usually derived from volcanic ash soils mainly used for upland drops, it may influence the nitrate-nitrogen concentration of groundwater. The amount of rainfall also influence the water quality. But the COD_(Cr), were shown April (3.17㎎/L) > July (2.91 ㎎/L) > October (2.40 ㎎/L), it is highly related in the basal dose of organic matter fertilizers. This study demonstrated that groundwater quality was suitable for irrigation, but continuous monitoring is recommended for agricultural policy and developing OECD agricultural environment indicators.

인공 치아우식 발생 모델에서 디지털 방사선 공제술을 이용한 인접면 치아우식증의 진단

박정훈,최용석,황의환,이기자,최삼진,박영호,김경숙,진현석,홍경원,오범석,박헌국 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.1

Purpose : The purpose of the experiment was to evaluating the diagnostic ability of dental caries detection using digital subtraction in the artificial caries activity model. Materials and Methods : Digital radiographies of five teeth with 8 proximal surfaces were obtained by CCD sensor (Kodak RVG 6100 using a size #2). The digital radiographic images and subtraction images from artificial proximal caries were examined and interpreted. In this study, we proposed novel caries detection method which could diagnose the dental proximal caries from single digital radiographic image. Results : In artificial caries activity model, the range of lesional depth was 572-1,374 μm and the range of lesional area was 36.95-138.52mm². The lesional depth and the area were significantly increased with demineralization time (p<0.001). Furthermore, the proximal caries detection using digital subtraction radiography showed high detection rate compared to the proximal caries examination using simple digital radiograph. Conclusion : The results demonstrated that the digital subtraction radiography from single radiographic image of artificial caries was highly efficient in the detection of dental caries compared to the data from simple digital radiograph.