Lipids Induce Release of Tumor-Promoting Exosomes from Hepatocellular Carcinoma Cells

( Eun Ju Cho ),( Hyo Yeong Lee ),( Joon Yeul Nam ),( Young Chang ),( Hyeki Cho ),( Young Youn Cho ),( Jeong-hoon Lee ),( Su Jong Yu ),( Yoon Jun Kim ),( Jung-hwan Yoon ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hypoxia enhances lipid droplets accumulation by HIF-dependent mechanism, leading to intra-tumoral fatty metamorphosis which is one of the characteristics of early-stage hepatocellular carcinoma (HCC). We investigated whether lipids and lipids-induced exosomes released from HCC cells can induce a tumor-promoting phenotype. Methods: Human HCC cell lines (Huh-7, SNU-761, and SNU-3058) were incubated with oleic acid (OA) or control vehicle. The released exosomes were isolated, quantified, and applied to HCC cells. Results: Incubation of Huh-7 and SNU-761 cells with OA increased proliferation and migration of cells in a fatty-acid binding protein 3 (FABP-3) dependent manner, whereas SNU-3058 cells did not respond to OA. Furthermore, OA upregulated FABP-3 mRNA expression in Huh-7 and SNU-761 cells, whereas its expression in SNU-3058 cells did not change. OA enhanced release of exosomes from Huh-7 and SNU-761 cells, and exosomes collected form these cells upregulated proliferation and invasion of cells. However, this was not observed in SNU-3058 cells. Exosomes released from OA-treated cells were reduced by the inhibition of rho-associated, coiled-coil-containing protein kinase 1 (ROCK1) or FABP-3. Conclusions: These findings suggest that lipids induce release of exo-somes containing unique cargoes from HCC cells in a FABP-3 dependent manner, which may induce the progression of HCC.

Lipids-Induced Exosomal lincRNA-ROR Released from Hepatocellular Carcinoma Cell Promotes Tumor Cell Growth through the PI3K/Akt Pathway

( Eun Ju Cho ),( Young Chang ),( Hyoyeong Lee ),( Jeong-hoon Lee ),( Su Jong Yu ),( Yoon Jun Kim ),( Jung-hwan Yoon ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: The lipid-related metabolic dependence is a hallmark of cancer. Exosomes in tumor microenvironment are enriched in lipids and their accumulation in cells might modulate recipient cell homeostasis. We investigated whether lipids and lipids-induced exosomes released from HCC cells can induce a tumor-promoting phenotype and the underlying mechanisms. Methods: Human HCC cell lines (Huh-7, SNU-761, and SNU-3058) were incubated with oleic acid (OA) or control vehicle. The released exosomes were isolated, quantified, and applied to HCC cells. Results: Incubation of HCC cells with OA enhanced proliferation, migration and invasion of cells. Furthermore, OA upregulated long noncoding RNA regulator of reprogramming (lincRNA-ROR) mRNA expression in Huh-7 and SNU-3058 cells, whereas its expression in SNU-761 cells did not change. Exosomes collected form OA-treated cells were enriched with lincRNA-ROR mRNA, and upregulated proliferation and invasion of HCC cells through the activation of PI3K/Akt pathway. Conclusions: These findings suggest that lipids-induced exosomal lincRNA-ROR released from hepatocellular carcinoma cell promotes tumor cell growth through the PI3K/Akt pathway.

심리전문가 수련생을 위한 집단상담 프로그램 개발의 예비연구: 심리도식 및 수용전념치료를 중심으로

조주성(Ju Sung Cho),장은진(Eun Jin Chang),전은영(Eun Young Jeon) 한국발달지원학회 2020 발달지원연구 Vol.9 No.3

본 연구는 심리전문가 수련생을 대상으로 전문가로서의 성장을 목적으로 심리도식치료 및 수용전념치료에 기반한 집단상담 프로그램 개발을 위한 예비연구를 실시하고 그 효과성을 검증하고자 하였다. 이를 위하여 프로그램 개발에 관한 모형을 통해 관련 변인과 총 18회기의 내용을 개발하고 내용타당도를 검증하였으며, D지역 심리전문가 수련생 7명을 대상으로 해당 프로그램을 실시한 후 대응표본 t검증과 효과크기(Cohen’s d)를 산출하여 효과성을 검증하였다. 그 결과, 자기성찰(t=-2.04, df=6, p=.04), 정서인식의 명확성(t=-3.16, df=6, p=.01), 정서적 안정성(t=2.81, df=6, p=.01)이 통계적으로 유의미한 변화를 나타내었으며, 자기수용은 유의미한 수준의 변화를 나타내지는 않았지만(t=-.1.34, df=6, p=.11), 효과크기는 중간수준임으로 나타나(d=-0.50) 더 많은 대상자들을 상대로 실시할 경우, 통계적으로 유의미한 결과를 산출할 가능성이 있음을 확인하였다. 본 연구는 심리도식치료와 수용전념치료를 적용한 집단상담 프로그램을 통하여 심리전문가 수련생들에게 교육분석에 필요한 내용을 체계적으로 제공하고 그 효과성을 검증하였다는 점에서 의의가 있다. 본 예비연구에서 효과성이 검증되었으므로 향후 프로그램의 완성도를 높인 후, 비교집단을 활용한 무선 통제집단연구나 종단연구 등을 통하여서도 반복적인 효과성 검증을 할 것을 제안하였다. The purpose of this study is to develop and verify the effectiveness of group counseling program, based on the Schema Therapy(ST) and the Acceptance-Commitment Therapy(ACT), for the growth of psychological professional trainees. For this purpose, psychological professional trainees(n=7) were surveyed, and research participants were selected. Based on program development model, this study conducted content validity verification, conducted a paired samples t-test, and calculated effect size (Cohen s d) to verify the effectiveness of the 18 session program. As a result, introspection (t=-2.04, df=6, p=.04), clarity of emotional recognition (t=-3.16, df=6, p=.01), and emotional stability (t=2.81, df=6, p=.01) showed statistically significant changes. Although acceptance (t=-1.34, df=60, p=.11) did not show a significant change, its effect size being middle level(d=-0.50) indicates that if the test is conducted in a larger sample, a statistically significant outcome could result. This study is meaningful in that it verified the effectiveness of a systematic expert training program that applies the ST and the ACT. Since the program’s effectiveness has been verified in this preliminary study, it is suggested that a controlled or longitudinal study should be conducted to re-verify the effectiveness of this program.

인삼을 이용한 가공방법 개발과 효능검증

예은주,배만종,김수정,박창호 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

본 연구는 인삼의 가공방법을 개발하고 제품을 다양화하기 위한 기초 자료를 확립하고자, 우리나라의 전통주인 약주로 증자한 후 홍삼의 일반성분과 ginsenoside의 함량을 분석하고 갈색도 및 탁도의 변화를 알아보았다. 또한 항암효과를 검증하였던바 그 결과를 보고하고자 한다. 1. 백삼과 홍삼, 증자 횟수별 인삼의 갈색 변화를 보기 위해서 각 인삼의 60% 에탄올추출물의 갈변의 측정 결과 갈변의 전구물질과 중간생성물 및 fufural, 갈색계의 색소, 탁도 모두 증자횟수가 증가 할수록 값이 증가하였다. 그리고 백삼, 홍삼 A1는 3가지 항목에서 모두 비슷한 경향을 보였으며, 상대적으로 A3에서 높은 값을 보였다. 2. HPLC상으로 total ginsenoside의 함량을 측정한 결과 백삼 28.20mg/g, 홍삼 421.10mg/g이고 A1~A9에서는 46.10~76.80mg/g으로 홍삼과 백삼보다 증자 횟수가 증가 할 수록 total ginsenosied 함량이 증가하였다. 3. HPLC상에서 분석한 증자 횟수별 인삼의 ginsenosides(G-Rb₁, -Rb₂, -Rc, -Rd, -Re, -Rf, -Rg₁, -Rg₂, -Rg₃)의 함량변화는 G-Rc, -Rd, -Re는 1회 증자한 A1에서 함량이 증가 하다가 증자 횟수가 증가 할수록 다시 함량이 감소하였으며, G-Rg₂, -Rg₃는 증자 횟수와 비례하여 함량이 증가하는 경향을 보였다. 4. 인체유래 간암세포(Hep3B)에서의 항암효과 검증실험에서는 물, 60%에탄올 추출물을 각각 1000ppm, 2500ppm, 5000ppm으로 처리 한 후 세포 증식 억제율을 확인하였다. 백삼은 모든 처리군에서 미미한 증식억제 효과가 있었으나, 홍삼은 60%에탄올 추출물 5000ppm에서 54.52%, A9에서는 96.28%의 현저한 세포증식억제 효과가 있었다. A9가 Hep3B에 대해서 백삼과 홍삼보다 세포 증식 억제율이 더 높은 것을 확인 할 수 있었다. 이상의 결과로 볼 때 약주를 이용해서 증자한 홍삼이 백삼이나 일반홍삼보다 총 사포닌, 진세노사이드의 함량이 증가하였고, 기능과 효능이 증강된 것으로 확인되어 우수함이 입증되었다. 또한, 홍삼정과, 절편정과 등 제품의 다양화와 품질개선에 기여할 것으로 기대된다. 향후 증자 방법, 압력, 온도, 및 약주 등의 제조 조건에 따른 지속적인 연구가 요청된다. This study takes place to establish basic data to develop various ginseng-processing methods and products. It analyzed components and quantity of ginsenoside in red ginseng after brewing with the traditional medicinal wine as well as the brownness and impurity level changes, and verified anti-oxidization and anti-cancer effects. The following is the founding of these analyses. To examine the changes in the brownness of white and red ginseng, premonitory materials of 60% ethanol extracts were examined As the result of these examinations, all of the values of examined matters premonitory materials, mid products, Fufural, coloring matters and impurity level were increased as the brewing continued. And the brownness level for white and red ginseng was similar in Al and the values were relatively high in A3. The total Ginsenoside on HPLC was 28.20㎎/g for white ginseng and 42.10㎎/g for red ginseng. The quantity of total Ginsenoside was increased as the brewing continued as 46.10∼76.80㎎/g was shown in A1∼A9. To see the quantity changes of Ginsenosides(G-Rbi, -Rte, -Re, -Rd, -Re, -Rf, -Rg₁, -Rg₂, -Rg₃) contained in ginseng on HPLC, the quantity of G-Rc, -Rd, -Re were increased in Al the first brewing and decreased as the brewing continued. The quantity of G-Rg2, -Rg3 was increased proportionate to the number of times for brewing. In an anti-cancer test in liver cancer cells(Hep3B) extracted from human body, each of water and 60% ethanol extracts were processed with 1000ppm, 2500ppm, and 5000ppm and checked the multiplication restrain rate. All of the processing groups in white ginseng have minimum multiplication restrain rates, but red ginseng has 54.52% at 60% ethanol extract 5000ppm. At A9, 96.28%) of cell multiplication restrain effects were shown. Comparing Hep3B to A9, white ginseng has higher restrain effects than red ginseng. As seen in the above results, the reinforced red ginseng using the medicinal wine has higher total saponin and ginsenosides, as well as with increased functions and effects leading the superiority. And it is possible to use Red Ginseng Jung Kwa; Jung Kwas with flower pattern imprinted, and expect to increase the opportunities to use the extracts. Later, when brewing the pressure, temperature, and medicinal wine can be examined to continuous studies.

인구통계학적 특성 및 한옥 경험에 따른 한옥이미지와 행동의도 차이 분석

장영화(Young Hwa Chang),김은주(Eun Ju Kim) 한국관광연구학회 2014 관광연구저널 Vol.28 No.5

The study aims to investigate the image of Hanok, the traditional Korean house, and see a gap between the image of Hanok and behavioral intention of people depending on their experience of Hanok. The result shows that people have different ideas about functionality and symbolism of Hanok depending on their gender and symbolism of Hanok differs depending on the region it is located. As a result of investigating the changes of image and behavioral intention for Hanok after visiting it the image people have for Hanok do not change. But when it comes to behavioral intention people tend to revisit Hanok, stay overnight or live in it as they visit it more often. It has been found in the study that the image of Hanok is influenced only by gender and region but not by most of demographic factors. This means that it is possible to promote Hanok without identifying its target market. That most of the behavioral intention gets stronger as the frequency of visiting Hanok increases shows it is necessary to develop active contents to attract people and let them have a positive image of Hanok. The fact that how people feel about Hanok puts an influence on the image of Hanok suggests that positive image of Hanok related to emotion can lead to change people’s intention in the end.

Association Between Gut Regulatory Hormones and Post-operative Weight Loss Following Gastrectomy in Patients With Gastric Cancer

Hye-Kyung Jung,Chung Hyun Tae,Hye Ah Lee,Ko Eun Lee,Chang Mo Moon,Seong Eun Kim,Ju Young Seoh,Joo-Ho Lee 대한소화기 기능성질환·운동학회 2022 Journal of Neurogastroenterology and Motility (JNM Vol.28 No.3

Background/AimsPost-operative weight loss in patients with gastric cancer lead to a poor quality of life and long-term survival. This study aims to evaluate the effects of gut regulatory hormones on post-operative weight loss in patients with subtotal gastrectomy for gastric cancer. MethodsThis prospective study was conducted for 12 months post-surgery in 14 controls and 13 gastrectomy patients who underwent subtotal gastrectomy for gastric cancer. Serum plasma ghrelin, glucagon-like peptide-1, gastric inhibitory peptide-1, peptide YY, insulin, and homeostatic model assessment for insulin resistance responses to a standardized test meal were recorded at multiple time points before and after gastrectomy at 4 and 12 months. ResultsThe mean weight difference between the pre-operative state and the 4-month period was significantly reduced to 6.6 kg (P = 0.032), but significant weight reduction was not observed from 4 months to 12 months. The plasma levels of glucagon-like peptide-1, gastric inhibitory peptide-1, and peptide YY were significantly increased 4 months postoperatively compared to the pre-operative state (all P = 0.035); however, pre-operative levels and relative changes over a period of 0-4 months of hormones were not correlated with body weight changes. Only the pre-operative ghrelin at peak had a negative correlation with changes in weight reduction in the 4 months after surgery (ρ = −0.8, P = 0.024). ConclusionsSignificant weight reduction was common after subtotal gastrectomy for gastric cancer with a negative correlation pre-operative plasma ghrelin levels. Incretin hormones are modestly but significantly increased after subtotal gastrectomy; however, these changes did not affect the weight changes.

Telomere의 기능 손실에 의한 Telomerase 결핍 세포의 성장 억제에 대한 연구

주연진,박정은,전경미,김창민,김상훈,박원봉,이기호 대한암예방학회 2002 Journal of cancer prevention Vol.7 No.3

교내 자판기와 터치스크린에 분포하는 세균의 실태조사

조주영,박창은 남서울대학교 2012 남서울대학교 논문집 Vol.18 No.1

The distributed species and the kinds of viable microorganism in button or touch screen of machine was monitored according to coffee & soda machine and automated teller machine(ATM). Our purpose was to investigate the distribution of bacteria in button or touch screen of machine. Analysis of dispenser contamination was sampled from 8 location in Namseoul University for 3 months from September to November in 2011. The isolated bacteria were identified by Gram stain and biochemical test using VITEKⅡsystems. The experiment on microorganism concentration of contact parts was carried out and the average of total microorganism was measured. The Coffee & soda machine and ATM was isolated and identified. The isolation rates of Gram positive cocci, Gram negative bacilli were 40.1%, 26.9% respectively. The frequently isolated bacteria were S. epidermidis, S. haemolyticus, S. warneri, Micrococus luteus/ lylae, Sphinogomonas paucimobilis . Further systematic studies are necessary with an emphasis on species identification Key Words : Microorganism, Coffee & soda machine, ATM

Caffeine이 지구성 운동 수행능력에 미치는 영향과 억제성 아미노산에 의하여 활성화되는 이온통로의 조절작용

김은경,김영표,천병옥,이계영,김연정,임백빈,조영욱,김창주,김성수 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

To investigate the effect and mechanism of caffeine on endurance exercise, two experiments were performed. First, to test caffeine effect on aerobic exercise, 200-300g Sprague-Dawley rats were used and three groups, control group, low caffeine injection group and high caffeine injection group, were divided. Blood smpling by heart puncture were done at rest, after 30 min treadmill exercise, and after maximal exercise. Blood glucose, free fatty acid concentration were detected and following results were obtained. Glucose concentration showed significant difference between groups(p=0.0305) and also significant changes were exhibited between time(p=0.0004). Free fatty acid concentration had no difference between groups. but had significance between times(p=0.00065). Exercise endurance performance time showed significant difference(p=0.02350 in high caffeine injection group compared to control group. In this experiments, endurance exercise capacity was increased by caffeine injection. Therefore, second experiment was performed to investigate the effect of caffeine on ion current induced inhibitory amino acid neurotransmitter. GABA and glycine. Single periaqueductal gray neuron was acutely dissociated and nystatin perforated patch clamp was performed under voltage clamping condition. Caffeine evoked outward current in PAG neuron dose dependent manner. 1mM of caffeine application had no response. but 3mM caffeine evoked about 32.5±8.539pA outward ion current and 10mM caffeine evoked about 215.46±19.4pA outward current. 10^-2mM GABA activated Cl￣current and recorded by inward current. Caffeine inhibited GABA activated Cl￣ current concentration dependent manner. 10^-2mM of caffeine had no effect on 1-^-2mM of GABA response. but 10^-1mM caffeine inhibited GABA activated Cl￣ current about 5.74±2.13%, 1mM caffeine inhibited about 17.25±2.70%, 10mM caffeine inhibited GABA response about 45.31±7.71%. 10^-1mM of glycine activated Cl￣ current and also recorded by inward current. Caffeine inhibited glycine activated Cl￣ current concentration dependent manner. 10^-2mM caffeine decreased glycine activated Cl￣ current about 4.61±1.650%, 10^-1mM caffeine decreased about 6.49±2.24%, 1mM caffeine decreased about 26.82±4.27%, and 10mM caffeine decreased glycine response about 94.47±1.39%. These results suggest that caffeine inhibite inhibitory amino acid, GABA and glycine, this response causes excitation of CNS and this seems to be the basic mechanism of increasing effect to aerobic exercise performance by caffeine.

수면 무호흡증 환자의 진단방사선학적 고찰

손은주,박정희,전혜정,최영칠,이창희,신현준,이경용 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

Our purpose was to determine whether there are statistical differences in upper airway size and compliance when patients with obstructive sleep apnea are compared with healthy volunteers using ultrafast CT, and to evaluate the diagnostic accuracy of ultrafast CT in obstructive sleep apnea. 29 patients clinically suspected of obstructive sleep apnea and ten healthy volunteers underwent examination of the upper airway with ultrafast computed tomography. All patients had operations; uvulopalatopharyngoplasty (UPPP,n = 19), UPPP with tosillectomy (n = 7) and UPPP with septoplasty (n = 3). Eight axial images every 0.7 sec in 8 ㎜ sections were obtained using flow mode. CT was performed twice while the subjects were awake with quiet breathing and were asleep with apnea. Smallest cross-sectional area in the nasopharynx, oropharynx and hypopharynx and compliances were assessed in patients and control subjects. Among the 29 patients, 22 patients showed narrowing at oropharyngeal level and six patients showed narrowing at two or more levels. Patients with obstructive sleep apnea had significantly smaller mean cross-sectional area than obstructive sleep apnea had significantly smaller mean cross-sectional area than control subjects in the oropharynx while sleeping (p<0.05). But there was no significant difference in mean smallest cross-sectional area of the nasopharynx (p = 0.47). In addition, the patients with obstructive sleep apnea had greater compliance of the oropharynx (79.8% vs 39.3%) and hypopharynx (58.9% vs 19.2%) (p<0.01). Patients with obstructive sleep apnea are characterized by a small oropharyngeal airway and collapsible oropharyngeal, hypopharyngeal airways. Ultrafast CT scanning allows rapid and noninvasive assessment of airway variables.