Immunophenotypic Characterization and Quantification of Neoplastic Bone Marrow Plasma Cells by Multiparametric Flow Cytometry and Its Clinical Significance in Korean Myeloma Patients

조영욱,박찬정,박서진,지현숙,장성수,박상혁,서을주,윤덕현,이정희,서철원 대한의학회 2013 Journal of Korean medical science Vol.28 No.4

Multiparametric flow cytometry (MFC) allows discrimination between normal and neoplastic plasma cells (NeoPCs) within the bone marrow plasma cell (BMPC)compartment. This study sought to characterize immunophenotypes and quantitate the proportion of NeoPCs in BMPCs to diagnose plasma cell myeoma (PCM) and evaluate the prognostic impact of this method. We analyzed the MFC data of the bone marrow aspirates of 76 patients with PCM and 33 patients with reactive plasmacytosis. MFC analysis was performed using three combinations: CD38/CD138/-/CD45; CD56/CD20/CD138/CD19; and CD27/CD28/CD138/CD117. The plasma cells of patients with reactive plasmacytosis demonstrated normal immunophenotypic patterns. Aberrant marker expression was observed in NeoPCs, with negative CD19 expression observed in 100% of cases, CD56+ in 73.7%, CD117+ in 15.2%, CD27- in 10.5%, CD20+ in 9.2%, and CD28+in 1.3%. In PCM patients, more than 20% of NeoPCs/BMPCs were significantly associated with factors suggestive of poor clinical outcomes. Patients who were CD27- or CD56+/CD27-, demonstrated shorter overall survival than patients of other CD56/CD27combinations. Our results support the clinical value of immunophenotyping and quantifying NeoPCs in PCM patients. This strategy could help to reveal poor prognostic categories and delineate surrogate markers for risk stratification in PCM patients.

말초혈액 도말검사로 우연히 진단된 말레이 사상충증 2예

조영욱,성흥섭,이호종,김지훈,석웅,박호선,정태은,이영하 대한진단검사의학회 2005 Annals of Laboratory Medicine Vol.25 No.1

The Brugian filariasis in Korea was once endemic in several areas including Jeju-do and small remote islands located in the southwestern part of the Korean peninsula. But recently it has almost been controlled. The first patient was a 42-year-old man, who visited the hospital with the chief complaints of fatigue, jaundice and edema on the right low extremity. Examination of a peripheral blood smear revealed non-megaloblastic macrocytic anemia, thrombocytopenia and eosinophilia, and a parasite, which was identified as microfilaria of Brugia malayi on the glass slide of blood obtained from the patient at midnight. The second patient was a 23-year-old man, who visited the hospital with the chief complaints of cough, sputum and fever. A parasite resembling that of the first patient was found in a peripheral blood smear accompaning neutrophilia. No more parasites, however, were found in the peripheral blood obtained from the patient at midnight. These two clinical cases of Brugian filariasis indicate that the clinical laboratory in Korea should be able to detect microfilariae for the diagnosis of filariasis. 우리나라에서 말레이 사상충증은 과거 제주도와 한반도 남서부의 낙도지역에서 유행한 적이 있었으나, 현재 대부분 지역에서현저히 감소하였다. 첫째 예는 42세된 남자로 피곤함, 황달 그리고 우측 발등의 부종을 주소로 내원하였다. 말초혈액 도말검사에서 비거대적혈모구 대적혈구성 빈혈, 혈소판감소증, 호산구증다증과 함께 기생충이 관찰되었다. 자정에 채취한 말초혈액에서 말레이 사상충의 미세사상충으로 동정하였다. 둘째 예는 2세 남자로 기침, 가래 그리고 발열을 주소로 내원하였다. 말초혈액 도말검사에서 호중구증가증과 함께 첫째 예와 유사한 기생충이 관찰되었다. 그러나 자정에 채취한 말초혈액에서는 기생충이 발견되지 않았다. 두 증례를 통해 앞으로 국내 검사실에서도 사상충증의 진단에 대한 지식이 필요할 것으로 사료된다.

Resolution of Third Cranial Nerve Palsy Following Endovascular Treatment of the Posterior Communicating Artery Aneurysm

조영욱,황금,이명석,김헌주 대한신경외과학회 2004 Journal of Korean neurosurgical society Vol.35 No.1

Third cranial nerve palsy may indicate the presence of an intracranial aneurysm, most commonly in the posterior communicating artery. The effect of endovascular management of posterior communicating artery aneurysms on recovery from third cranial nerve palsy is not well documented. We report two patients with third cranial nerve palsy caused by posterior communicating artery aneurysms. Endovascular treatment resulted in subsiding of ptosis within two weeks. Resolution of extraocular muscle function occurred within one to three months after treatment. Endovascular treatment, which has recently been introduced into neurosurgical practice, is effective in preventing aneurysmal rupture, and in eliminating manifestations due to mass effects. Key words:Posterior communicating artery aneurysm;Endovascular treatment;Third cranial nerve palsy.

Photostability of 1,3,4-oxadiazole derivatives

조영욱,김형준 한국공업화학회 2012 한국공업화학회 연구논문 초록집 Vol.2012 No.1

자하차(紫河車)약침이 제2형 콜라겐 유발 관절염에 미치는 영향

조영욱,이승우,강민주,김은정,이승덕,김갑성,Jo, Young-Wook,Lee, Sung-Woo,Kang, Min-Joo,Kim, Eun-Jung,Lee, Seung-Deok,Kim, Kap-Sung 대한침구의학회 2007 대한침구의학회지 Vol.24 No.6

Objectives : We investigated the effects of Hominis placenta Herbal acupuncture on rheumatoid arthritis. Methods : We investigated the Type II collagen-induced arthritis test, vascular permeability test, phenyl benzoquinone-induced writhing test, hot plate test, carbon clearance test, determination of prostaglandin $E_2$ content and cyclooxygenase-2 activity test. Results : Hominis placenta Herbal acupuncture suppressed Type II collagen-induced arthritis in DBA/1J mice and vascular permeability with 10.2% inhibition in ICR mice. We see the phenyl benzoquinone-induced writhing test and the hot plate test in the analgesic activity test. In the phenyl benzoquinone-induced writhing test, Hominis placenta Herbal acupuncture showed that it increased its analgesic effect more than the control(test?) by 85.5%. In the hot plate test, it was also shown that the analgesic effect increased more than the control(test) by 32.1%. In the carbon clearance test, the immuno-stimulatory effect showed more than the control(test) by 8.0%. The formation of prostaglandin $E_2$ was also reduced more than the control(test) by 10.1%. Finally, cyclooxygenase-2 activity was inhibitedwith 36.3%. Conclusions : Hominis placenta Herbal acupuncture may be useful for the treatment of rheumatoid arthritis after injection.

Acuracy of Automated Urine analyzer (UF-1000i) in Detecting Dysmorphic RBC

조영욱,박세영,김영옥 대한내과학회 2017 대한내과학회 추계학술대회 Vol.2017 No.1

뼈모세포의 활성도에 대한 자성의 효과에 관한 연구

조영욱,최부병,이성복,Cho, Young-Wook,Choi, Boo-Byung,Lee, Seong-Bok 대한턱관절교합학회 2003 구강회복응용과학지 Vol.18 No.2

The object of this study is to observe the effects of magnetism on the osteoblasts using a neodymium magnet. The osteoblasts was cultured under magnetic fields of varying intensities to evaluate the effect of magnetism on the activity and alkaline phosphatase acitivty of the osteoblasts. Osteoblasts were cultured in the cell density of $10^4$ for the evaluation of cell proliferation and 105/ml for the evaluation of ALP activity under 0. 10, 100, 500, 1000, 2000, 4000 gauss for 24 hour. For evaluation of osteoblast morphologic changes under magnetic, osteoblasts were observed by inverted microscope and TEM. To elucidate if IGF-receptors are increased under the magnetic field, we investigated osteoblasts by immunofluoroscence staining. The results were as follows: In the varying intensities of magnetic fields, the degree of cell proliferation was the highest in the magnetic field of 10 gauss and this gradually decreased up to 1000 gauss. In the magnetic fields stronger than 1000 gauss, the degree of the cell proliferation decreased to an even lower level than that of the control group. The ALP activity and protein synthesis showed a similar increase pattern as the degree of cell proliferation compared to the control group but showed little difference. Under the microscope, morphological change of the cells ( decrease in length and increase in roundness) were observed but no peculiarity of cell distribution could be found according to the magnetic field line. In the proper intensity of magnetic fields (10 gauss), the cultured cells showed increase in number of IGF Receptors compared to that of the control group.

정상 한국인 집단과 허혈성 뇌혈관질환 환자군에서의 응고인자 XII 46C/T 다형성 분석

조영욱,지현숙,최수진,심은희,박찬정 대한진단검사의학회 2002 Annals of Laboratory Medicine Vol.22 No.4

배경 : 동양인의 응고인자 XI 혈장농도는 서양인의 1/2 정도밖에 되지 않는다고 한다. 최근 응고인자 XI 유전자 엑손 1의 46번째 시토신(cytosine)이 티미딘(thymidine)으로 치환된 돌연변이인 경우 응고인자 XI의 혈장농도를 낮추는 결과를 초래한다는연구결과가 보고된 바 있다. 한편 몇 선천적 응고인자 XI 결핍증 환자들에서 혈전성향을 나타내는 것으로 알려져 있다. 본연구에서는 정상 한국인에서의 응고인자 XI 활성도와 46C/T다형성과의 연관관계를 조사하고, 허혈성 뇌혈관질환 환자군에서이러한 다형성 분포가 어떠한 연관성을 갖는지 알아보고자 하였다.방법 : 총 34명의 건강한 성인과 163명의 환자들이 연구대상이 되었다. 응고인자 XI의 활성도는 응고인자 XI 결핍혈장을이용한 aPTT 방법으로 측정되었고, 유전자형은 중합효소연쇄반응과 제한효소Hga I을 처리하여 분석하였다.결과 : 대조군에서의 유전자형 분포는 C/C, C/T, T/T 순서로 7.8%, 47.3%, 44.9%이었다. 대조군과 환자군 간의 유전자형분포는 통계학적으로 유의하지 않았다(P=0.800). 대조군에서의각 유전자형별 응고인자 XI의 활성도는 C/C, C/T, T/T 순서로 129.4%, 92.4%, 50.2%로, T 대립인자가 증가할수록 유의하정상 한국인 집단과 허혈성 뇌혈관질환 환자군에서의 응고인자 XII 46C/T다형성 분석 229게 낮아지는 결과를 보였다(P<0.05). 환자군에서도 동일한 결과를 보였다. 그러나 환자군에서 연령에 따른 활성도의 변화는 역비례 관계를 보였고(r=-0.225, P=0.016), 61세 이상인 환자군에서의 T/T유전자형이 동일 연령군에서의 대조군보다 유의하게

標本 根結 氣街의 定義와 鍼灸治療적 적용에 관한 연구

조영욱,조현석,황민섭,김갑성,이승덕 대한침구의학회 2005 대한침구의학회지 Vol.22 No.1

Objective : This study is present studies of Definition and Acupuncture Treatment of Biaoben(標本), Geungyul(根結) and kika(氣街). Methods : We studied oriental medical books and journal related with Biaoben(標本), Geungyul(根結) and kika(氣街). Results and Conclusion : 1. Theory of Biaoben(標本), Geungyul(根結) and kika(氣街) is a ground of remote acupuncture point needling. 2. Ben(本) and Geun(根) at distal part of limbs have effect a disease of haed, face, chest and back.

다발골수종 환자에서 골형성 생화학적 지표의 임상적 유용성

조영욱,민원기,박찬정,지현숙 대한진단검사의학회 2005 Annals of Laboratory Medicine Vol.25 No.5

Background : Bone lesions in multiple myeloma (MM) are related to an uncoupling of bone resorption and formation. This study was to evaluate the potential clinical value of biochemical markers of bone formation in patients with MM. Methods : Serum bone-specific alkaline phosphatase (BAP) and osteocalcin (OC) were measured in 217 sera from 77 patients with MM, 1 patient with plasma cell leukemia, and 12 patients with monoclonal gammopathy of undetermined significance (MGUS); 30 age- and sex-matched controls were also enrolled. Results : Serum OC, but not serum BAP, was lower in MM and MGUS patients than in controls. Serum BAP decreased significantly as the tumor burden increased (P=0.035), but serum OC did not decrease. On the other hand, serum OC increased significantly in patients in an advanced stage according to the new international staging system (P=0.036), whereas serum BAP did not exhibit such a change. Serum OC and BAP showed positive correlations with albumin; serum OC also showed positive correlations with 2-microglobulin and creatinine. Conclusions : Serum BAP, rather than serum OC, appears to be helpful in monitoring MM. The clinical usefulness of serum BAP may be enhanced in MM patients with renal impairment. 배경 : 골수종에서의 골병변은 골흡수와 골형성의 불균형과 연관되어 있다. 이에 저자들은 다발골수종 환자에서 골형성 표지자의 임상적 유용성을 평가하였다.방법 : 다발골수종 환자 7명, 형질세포 백혈병 환자 1명, mon-oclonal gammopathy of undetermined significance (MGUS) 환자 12명에서 채취한 217개의 혈청에서 골형 알칼리성인산분해효소(bone-specific alkaline phosphatase, BAP)와 오스테오칼신(os-teocalcin, OC)을 측정하였다. 대조군으로 골수종환자군과 연령및 성별이 일치하는 30명을 포함시켰다.결과 : 혈청 OC는 다발골수종과 MGUS 환자군에서 대조군보다 유의하게 낮았으나 혈청 BAP는 유의한 차이가 없었다. 혈청BAP는 종양부하량이 증가할수록 유의하게 감소하였으나(P=0.035), 혈청 OC는 유의한 차이가 없었다. 반면 새로운 국제 병기분류 체계를 참조한 분류에서는 혈청 OC가 질병의 진행단계가 높아질수록 유의하게 증가하였으나(P=0.036), 혈청 BAP는 유의한차이가 없었다. 혈청 OC는 알부민, 2-microglobulin, 크레아티닌과 유의한 양의 상관관계를, BAP는 알부민과 유의한 양의 상관관계를 보였다. 결론 : 혈청 OC보다 BAP가 다발골수종 환자의 경과추적에 도움이 될 수 있을 것이라 사료되었다. 특히 신기능이 저하된 환자들에게서 더 유용할 것이라 기대된다.