한국인에서 NOTCH4 유전자 내 두 개의 단일염기다형성과 정신분열병의 연합연구

주은정,정성훈,김미재,구영진,배승오,안용민,이규영,김용식 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : Previous studies on N0TCH4 gene and schizophrenia have not produced consistent results, and more studies with various ethnicities and populations were warranted. This study was performed with Korean population to find the role of the N0TCH4 gene in the development of schizophrenia. Methods : 235 schizophrenics and 236 normal controls participated in the study. Two SNPs (-1725 A/G and -25 T/C) on the N0TCH4 gene were investigated. Genotyping was done by Taqman assay, and statistical analysis was done by contingency chi-square test for the allele and genotype frequencies and PowerMarker V3.0 for the haplotype. Results : The two SNPs did not deviate from Hardy-Weinberg equilibrium in neither schizophrenics or normal controls. Two groups were not different in terms of allele and genotype distribution for both SNPs. Two SNPs were found to be in linkage disequilibrium. Haplotype analysis could not find an association between schizophrenia and these two SNPs. There was no association between the age at onset and the genotypes for both SNPs. Conclusion : We could not find any significant association between schizophrenia and the N0TCH4 gene in this Korean population. Although there are limitations in this study, this result supports the conclusion that the N0TCH4 gene is less likely to play a major role on the development of schizophrenia in the Asian population.

한국인 만성 긴장형두통 환자에서 바이오피드백을 활용한 자율적 이완훈련의 효과

강은호,안주연,구문선,박주언,유범희 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.3

Objectives : We aimed to evaluate the efficacy of biofeedback-assisted autogenic training for chronic tension-type headache, and to determine the relationship among the changes in electromyography (EMG) activity, headache activity, and mood states according to the psychophysiological treatment. Methods : Chronic tension-type headache patients aged from 20 to 40 years (n=35) were randomized to the treatment group receiving biofeedback-assisted autogenic training (8 sessions) or the monitoring-only control group. EMG activities, headache index, and various psychological variables were examined. Results : We found greater treatment response rate (≥50% reduction in headache index) in patients with biofeedback-assisted autogenic training than in the monitoring group (61% vs. 18% ; χ²=6.882, df=1, p=0.01). There were no significant changes in the mean values of the pretreatment EMG activities across the sessions m either group (all p’s>0.1). Mood states including anxiety and depression improved over time in the both groups, with the improvements being more prominent in the treatment group. Moreover, the reduction in depression level predicted treatment outcome in terms of headache index (95% confidence interval : 0.272-0.966, p=0.039). Conclusion : These results show that biofeedback-assisted autogenic training is effective for the treatment of chronic tension-type headache in a Korean population. Changes in mood states may be closely associated with the clinical outcome in the treatment of chronic tension-type headache using biofeedback-assisted autogenic training.

백서 뇌에서 전기경련 충격에 의해 발현이 조절되는 새로운 유전자

정선주,박정은,강웅구,구영진,박주배,김용식 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.5

연구목적: 이 연구는 백서 뇌에서 전기경련 충격(Electroconvulsive shock, ECS)에 의해 발현이 조절되는 새로운 유전자를 검색하고, 발달단게 및 뇌 조직에 따른 발현양상을 관찰하기 위하여 시행되었다. 방법: 생후 7일, 21일된 어린 웅성 백서에 전기경련 충격을 가한 후 mRNS differential display-PCR(DD-PCR) 기법을 시행하여 발현의 차이를 나타내는 유전자를 검색한 후 서열분석을 실시하였다. 생후 7일 및 21일된 백서의 뇌와 성년 백서의 대뇌피질, 해마를 이용한 Northern blot 분석을 시행하여 검색된 유전자의 ECS 처치 후 시간경과에 따른 유전자 발현양상을 관찰하였다. 결과: ECS 처치군과 위충격군간에 발현의 차이를 나타낸 유전자 4개를 검색할 수 있었으며, 이중 ECS에 의해 발현이 증가되는 1개의 유전자를 선정하여 서열분석을 실시한 결과 EST 서열만이 보고된 새로운 유전자임이 밝혀졌다. 이 유전자는 생후 7일된 백서의 뇌에서는 ECS 처치 후 180분에 발현양의 증가가 최고치에 도달하였으나, 생후 21일된 백서의 뇌와 성년백서의 대뇌피질 및 해마에서는 360분 경과시까지 지속적으로 발현 양이 증가하였다. 결론: 이같은 결과는 ECS에 의해 생체 내에서 유전자 발현의 조절이 이루어짐을 시사하며, 향후 이러한 유전자의 세포 내 기능에 관한 연구를 시행함으로써, ECS에 의한 생체의 반응과 치료기전에 대한 이해를 넓힐 수 있을 것으로 기대된다. 중심단어:전기경련 충격·DD-PCR·유전자·백서 뇌. Objectives: This study was performed to identify genes regulated by electroconvulsive shock (ECS) and to observe the pattern of expression of genes according to different developmental stages and brain regions. Method: ECS(130V, 0.5 sec) was given to male Sprague-Dawley rats with age of postnatal day 7 and 21(P7, P21 respectively). After screening genes regulated by ECS with mRNA diffe-rential display-PCR(DD-PCR), we selected one clone among them and observed the induction of this gene after ECS by time-dependent Northern blot analysis of rat brain of P7, P21 and adult rat cortex and hippocampus. Results: By DD-PCR method, we have identified four clones whose expression was regulated by ECS. Among them, one(CP 10-2) was proved to be a new gene by sequencing and BLAST search. Its expression was increased after ECS in P7, P21, and adult rat brain. The expression of CP 10-2 reached peak level at 180 minutes after ECS in P7 rat brain, but was further increased until 360 minutes after ECS in P21 and adult rat brain. Conclusion: In this study, a new gene was identified in rat brain which showed up-regulated expression in response to ECS. Cloning and characterization of this new gene would be helpful to elucidate the effect of ECS in rat brain. KEY WORDS:Electroconvulsive shock·Differential display-PCR·Gene·Rat brain.

Cordyceps scarabaeicola 의 약리활성

원소영,정현주,구혜진,소지현,박은희 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.20 No.-

Dongchunghacho (Dong-Chong-Xia-Cho in Chinese), one of folk medicines, is traditionally believed to be effective against various diseases. It includes many different genera such as Cordyceps, Paecilomyces, Torrubiella and Podonectria. Cordyceps scarabaeicola is one of well-known species. The 70% ethanolic extracts were prepared from two different sources pf C. scarabaeicola, fresh fruiting bodies (FFB) and mycelial cultured cells (MCC). Anti-angiogenic activity was determined by the chick embryo chorioallantoic membrane assay. Both FFB and MCC were found to contain strong anti-angiogenic activities. The extracts at the dose of 10 μg showed anti-angiogenic activity comparable to that of retinoic acid (dose, 1 μg) used as a control agent. Anti-angiogenic activities of FFB and MCC appeared to be concentration-dependent. No significant differences were found between the effects of FFB and MCC. These results might support the plausible anti-tumor activity of C. scarabaeicola. FFB also contains strong analgesic activity.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Validation and Application of a Qualitative Real-Time Polymerase Chain Reaction Method for Detecting Genetically Modified Papaya line 55-1 in Papaya Products

Yu Jihn Kwon,So Young Chung,Eun Joo Koo,Ji Eun Park,Dong Hyuk Seo,Yo A Lee,Yu Young Jung,Hee Eun Min,Mi Ran Kim,Eungui Kang,Jeongyun Cho,Seong Soo Park,Sun Ok Choi,Chul Joo Lim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Genetically modified (GM) papaya (Carica papaya L.) line 55-1 (55-1), which is resistant to papaya ringspot virus infection, has been marketed internationally. Many countries such as the European Union, Japan, and Korea have a mandatory safety assessment, approval and labeling regulations for GM foods. Thus, there is a need for specific methods for detecting 55-1. In this study, we established a real-time PCR detection method applicable to 55-1 for a variety of papaya products. The limit of detection was possible for fresh papaya fruit up to dilutions of 0.005% and 0.01% (weight per weight [w/w]) for homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The 55-1 event-specific detection method observed parallelism (r2>0.99) between the concentration of line 55-1 cultivars and Ct values obtained in amplification plots at concentrations of 0.005-10% for SunUp DNA and 0.01-10% for Rainbow DNA. The method was applicable to the qualitative detection in various types of processed products (cocktail fruit, dried fruit, juice, etc.) containing papaya as a main ingredient. Monitoring papaya products for the presence of GM papaya were demonstrated using a P35S and T-nos real-time PCR detection method but no amplification signals were detected.

Monitoring of illegally adulterated compounds in dietary supplements using UPLC and LC-QTOF/MS

Eun Jeong Cho,Ji Eun Lim,Eun Joo Koo,Jae Koan Seo,Sung Hee Park,Ji Yun Kim,Soo Yeon Choi,Mihyune Oh,In-Ho Kang 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Adulteration of dietary supplements with active pharmaceutical ingredients continue to be reported. Theses illegally adulterated compounds may have unintentionally entered from plants or intentionally added for immediate effects. Either way, an abuse intake of these compounds through food can cause serious side effects. In this study, we investigated 76 compounds including antiobestic drugs, antidiabetics, diuretics, analgesics, tranquillizer, selective androgen receptor modulators and their analogues in dietary supplements. We purchased 109 food samples from overseas internet sites, especially those marketed for weight loss and muscle building performance. The analysis of 76 compounds in samples was performed using UPLC-PDA and confirmed with LC-QTOF/MS. In 53 of 109 samples, 16 different illegal compounds were detected. The most frequently detected compounds were yohimbine (25.9%), synephrine (22.2%) and sennosides (11.1%). As the adulteration of dietary supplements has become a public health concern, further monitoring studies of adulterated foods are needed for ensuring food safety.

The impact of early and late acute rejection on graft survival in renal transplantation

( Eun Hee Koo ),( Hye Ryoun Jang ),( Jung Eun Lee ),( Jae Berm Park ),( Sung Joo Kim ),( Dae Joong Kim ),( Yoon Goo Kim ),( Ha Young Oh ),( Woo Seong Huh ) 대한신장학회 2015 Kidney Research and Clinical Practice Vol.34 No.3

Background: Advances in immunosuppression after kidney transplantation have decreased the influence of early acute rejection (EAR) on graft survival. Several studies have suggested that late acute rejection (LAR) has a poorer effect on longterm graft survival than EAR. We investigated whether the timing of acute rejection (AR) influences graft survival, and analyzed the risk factors for EAR and LAR. Methods: We performed a retrospective cohort study involving 709 patients who underwent kidney transplantation between 2000 and 2009 at the Samsung Medical Center, Seoul, Korea. Patients were divided into three groups: no AR, EAR, and LAR. EAR and LAR were defined as rejection before 1 year and after 1 year, respectively. Differences in graft survival between the three groups and risk factors of graft failure were analyzed. Results: Of the 709 patients, 198 (30%) had biopsy-proven AR [EAR¼152 patients (77%); LAR¼46 patients (23%)]. A total of 65 transplants were lost. The 5-year graft survival rates were 97%, 89%, and 85% for patients with no AR, EAR, and LAR, respectively. These differences were significant (Po0.001 for both by log-rank test). In time-dependent Cox regression analysis, EAR (hazards ratio, 3.37; 95% confidence interval, 1.90-5.99) and LAR (hazards ratio, 5.32; 95% confidence interval, 2.65- 10.69) were significantly related to graft failure. When we set LAR as standard and compared it with EAR, there was no statistical difference between EAR and LAR (P¼0.21). Conclusion: AR, regardless of its timing, significantly worsened graft survival. Treatments to reduce the incidence of AR and improve prognosis are needed.

Analytical Method Development for the Determination of Astaxanthin by LC-MS/MS in Dietary Supplements

Ji Eun Park,Mi Hyune Oh,Yang Sun Kim,Jin Hee Lee,Eun Joo Koo,Eun Hee Keum,Mee Hyun Cho,Mi Sook Kim,Ga Young Jung,Hye Young Lee 한국분석과학회 2018 학술대회논문집 Vol.2018 No.11

The Effects of KR-10876, a new Quinolone Antimicrobial Agent, on the Central Nervous System

Kim, Eun-Joo,Cha, Shin-Woo,Shin, Hwa-Sup,Roh, Jung-Koo,Park, Myoung-Whan,Kim, Wan-Joo The Pharmaceutical Society of Korea 1993 Archives of Pharmacal Research Vol.16 No.1

To evaluate KR-10876, a new fluoroquinolone antibacterial agent, its effects on the central nervous system(CNS) were investigated in mmice as part of phamacological study, and the results were compared with those for ciprofloxacin and ofloxacin, two prototypes of quinolone antiabctrial agents. All the parameters indicative of CNS function and acute toxicity were measured by close observation of the animals at regular time intervals after oral treatment of test compounds. KR-10876 did not have any effect on the parameters measured at lower does (100, 300 mg/kg, p.o.), it caused ptosis, suppressed spontaneous locomotor activity, hypothemia, and prolonged hexobarbital-induced sleeping time. KR-10876 also had a slight effect on motor coordination only at high dose. Simialr to ciprofloxacin, KR-10876 did not protect mice from pentylenetetrazol-strychnine-, and electroshock-inducedl convulsions at doses tested. These findings demonstrate that KR-10876 affects CNS functions only at high doses. The rank order for effects is ofloxacin$\le$KR-10876>ciprofloxacin.