대장암종에서 암관련 유전자들의 Promoter Methylation과 암종 발생 및 진행과의 관게에 대한 연구

정동준,이정은,민영기,조성우,백무준,양승하,김의한,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The colorectal corcinoma is one of the most common malignant tumor in Korea and its carcinogenic mechanism has not been elucidated exactly yet. So far, the most common carcinogenic mechanism has been known to be multiple genetic changes. But, the CpG island hypermethylation has been revealed to be one of the most important carcinogenic mechanism second to the genetic changes. The epigenetic changes characteristically imprint and transfer to the next generation without changes of nucleotide sequences. When the CpG island of promoter or 5'- exon 1 of a specific gene is methylated by DNA methyltrasferases (DNMTs), the gene expression is inhibited. Carcinogenic mechansisms by promoter methylation has been well known, but the progression of the cancer has not been elucidated yet. The molecular researches on the carcinogenic mechanisms largely depended on the tumor tissue containing various components of cell, due to difficulties in harvesting pure homogeneous tumor cells. Promoter methylatonof the cancer related genes as APc, hMLH2, p16, DAP-K and MGMT in 50 cases of sporadic colorectal carcinoma was studied. The pure normal colorectal epithelial cells, and cancer cells from superficial and deep invasive portion were harvested respectively by laser captured microdissection(LCM), and DNA was extracted from them. The promoter methylation was studied by methylation specific poymerase reaction (MSP) after bisulfite modification of the DNA and the expression of the genes by immunohistochemistry. The results suggests that promoter methylation of the cancer related genes are closely related with the colorectal carcinogenesis and it occurrs from early phase of carcinogenesis, progressing constantly to the late stage. And the promoter methylation of normal epithelial cells increased by age. The p15, known to be the genes related to hematologic malignancy, was closely related to the colorectal carcinoma as well.

Solid Modeling 기법을 응용한 복합곡면 가공에 있어서 공구간섭 제거

장동규,조시완,양균의 한국공작기계학회 1996 한국생산제조학회지 Vol.5 No.2

Compound surface modeling is widely used for die cavities and punches. A compound surface is defined in 3-D space by specifying the topological relationship of several analytic surface elements and a sculptured surface. A constructive solid geometry scheme is employed to model the analytic compound surface. the desired compound surface can be accomplished by specifying topological relationships in terms of boolean relations between primitives and the sculptured surfaces. Additionally, a method is presented for checking and avoiding the tool interference occurred in machining the compound surface. Using this method the interference of concave, convex, and side region can be checked easily and avoided rapidly.

전형적인 DISIDA scan 소견의 Rotor 증후군 1예

황의태,염동한,권지혜,임종주,최창수,김태현,김학철 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

만성의 비용혈성 직접형 고빌리루빈혈증은 Rotor 증후군과 Dubin-Johnson 증후군의 특징적인 소견으로, Rotor 증후군은 드문 양성 유전성 질환이며, Dubin-Johnson 증후군과 감별을 필요로 한다. 본 저자들은 황달을 주소로 내원한 26세 남자 환자에서 고빌리루빈혈증에 대한 검사를 시행하여 담도폐쇄 소견없이 간담도 스캔상 전형적인 Rotor 증후군의 소견 즉, 강한 심장 배후방사능이 지속되면서, 간담도계의 조영은 지연 촬영에서도 관찰되지 않으며 신장으로의 배설이 계속 관찰되는 특징적인 소견과 정상 간조직 검사 결과로 Rotor 증후군으로 진단한 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Chronic nonhemolytic conjugated hyperbilirubinemia characterizes both Rotor and Dubin-Johnson syndromes. Rotor syndrome is a rare, benign familial disorder and needs to be differentiated from Dubin-Johnson syndrome. Cholescintigraphy is a simple and informative procedure for the diagnosis, which can differentiate Rotor syndrome from Dubin-Johnson syndrome. A 26-year-old man was admitted due to asymptomatic persistent jaundice. Physical examination revealed icteric sclera without hepatosplenomegaly. Laboratory findings showed increased serum bilirubin with direct bilirubinemia. Markedly decreased hepatic uptake and poor visualization of the gallbladder and biliary tract were shown in ^(99m)Tc-DISIDA scan. Histology of the liver showed no diagnostic abnormality without pigmentation. But, we report a case of Rotor syndrome that showed characteristic cholescintigraphic findings with normal urinary total coproporphyrin excretion.

고밀도 플라즈마에 의해 식각된 SrBi_2Ta_2O_9 박막의 표면 반응 연구

김동표,김창일,장의구 중앙대학교 기술과학연구소 2000 기술과학연구소 논문집 Vol.30 No.-

본 연구에서 SBT 박막은 Cl_2양을 변화하면서 Cl_2/Ar 가스 플라즈마를 이용하여 식각하였다. 최대 식각 속도는 Cl_2(20%)/Ar(80%) 가스혼합비에서 883A˚/min 이었다. Cl_2 가스양이 증가함에 따라서 SBT 박막의 식각 속도는 감소하였다. 이는 Ar 이온의 물리적 스퍼터링에 의한 영향이 화확 반응에 의한 식각보다 우세하게 작용함을 의미한다. 식각 메커니즘을 규명하기 위하여 optical emission spectroscopy (OES), x-ray photoelectron spectroscopy (XPS), secondary ion mass spectrometry (SIMS)와 atomic force microscopy (AFM) 분석을 하였다. AFM 분석결과에서 Ar이나 Cl12 플라즈마를 이용하여 식각된 시료의 표면 거칠기 rms 값이 식각전의 시료나 Cl_2/Ar 플라즈마로 식각된 시료보다 크다. 이는 식각된 시료에서의 Bi 양의 감소나 비휘발성 식각 잔류물에 의한 영향이다. XPS와 SIMS 분석을 통하여 검증하였다. SBT thin films were etched at different content of Cl_2 in Cl_2/Ar. The maximum etch rate of SBT was 883A˚/min in Cl_2(20%)/Ar(80%). As Cl_2 gas increased in Cl_2/Ar gas plasma, the etch rate decreased. The result indicates that physical sputtering of Ar ion is dominant to chemical reaction in etching SBT thin films. To evaluate the etching mechanism of SBT thin films, optical emission spectroscopy (OES), x-ray photoelectron spectroscopy (XPS), secondary ion mass spectrometry (SIMS) and atomic force microscopy (AFM) were carried out. From the result of AFM, the rms values of etched samples in Ar only or Cl_2 only plasma were higher than that of as-deposited, Cl_2/Ar. This can be illustrated by a decrease of Bi content or nonvolatile etching products(Sr-Cl and Ta-Cl), which are revealed by XPS and SIMS.

폐자로형 평면 인덕터의 제조에 관한 연구

李昌昊,申東勳,南勝義 弘益大學校 科學技術硏究所 1998 科學技術硏究論文集 Vol.9 No.2

The purpose of this research were to develop the magnetic thin films using for high frequence range and to establish the processing technique of a meander type micro-coil in order to produce the micro magnetic device(MMD) and analyze its electrical characteristics. As magnetic thin films, we used Fe??Ta??N?? films which had nano-crystalline structure produced by a DC magnetron reactive sputtering system, magnetic properties were like these : Bs=15∼16 kG, Hc=0.12∼0.17 Oe, μ'=2800∼4000. The Cu coil produced by selective electro-plating(SEP) showed the resistivity of 2 μΩ-cm. In case of the meander type magnetic thin films inductor with 5 turns, an inductance of 229 nH which is about 4 times higher than that of air core inductor was shown, and a resonance frequency of 37 MHz and a quality factor of 1.8 at 10 MHz were also shown.

위선암에서 E-cadherin의 표출양상에 관한 연구

강동하,김대중,김창진,김의한,양승하 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

Gastric adenocarcinoma is the most common malignant tumor in Korea. Many oncogens have been found in malignant tumor cells. E-cadherin located in cell membrane is important for progression and metastasis of tumor. To evaluate the expression of E-cadherin according to histopathologic features and the relation between E-cadherin and prognosis on gastric adenocarcinoma, we have performed immune staining, using mouse HECD-1 for 97 cases. The results were as follows; 1) Gastric adenocacinoma showed the loss or marked reduction of the expression of E-cadherin in 30.9% of 97 cases, and early carcinoma 34.2% and advanced 28.8%. 2) According to differentiation of gastric adenocarcinoma, cases showing the loss or marked reduction of the expression of E-cadherin was well differentiated form 16.1%, moderately 35.7%, poorly 31.9% and mucinous carcinoma 100%. 3) According to the degree of invasion in early carcinoma, cases showing the loss or marked reduction of the expression of E-cadherin was carcinoma within mucosa 41.2% and carcinoma invaded to submucosa 28.6%, but there is no significant difference according to types. 4) According to absence or presence of lymph node metastasis in advanced carcinoma, cases showing the loss or marked reduction of the expression of E-cadherin was 17.6% in non-metastatic cases and 33.3% in metastatic cases, but there is no significant difference according to degree of invasion. Above results indicated that the loss or marked reduction of the expression of E-cadherin in gastric adenocarcinoma may be related to prognosis of the patients.

마이크로파 여기 플라즈마광 생성을 위한 도파관 시스템 설계에 관한 연구

전상재,전후동,송창현,하석영,이승혁,이태호,박의준 금오공과대학교 2005 論文集 Vol.26 No.-

In this paper, the plasma lighting system(PLS) excited by the commercial high power magnetron is developed. The design concepts are based on maximizing the huninous efficacy in conjunction with the miniaturization of waveguide system Furthermore the fine tuning is simplified by using only one stub, and the impedance matching is maximized by introducing the tapering technique. The experimental results show that the luminous efficacy can be dramatically improved by the proposed design method.

Development of vitrification protocol in Rubia akane (nakai) hairy roots using a systematic approach.

Park, Sang-Un,Kong, Hyunjung,Shin, Dong-Jin,Bae, Chang-Hwan,Lee, Sheong-Chun,Bae, Chang-Hyu,Rha, Eui-Shik,Kim, Haeng-Hoon Royal Veterinary College 2014 Cryo letters Vol.35 No.2

<P>A solution-based vitrification protocol is a process of sequentially changing-solutions from which both influx of cryoprotectants (loading) and efflux of water (dehydration) were accomplished before cryo-exposure. Hence, we need to properly control the concentration /composition of the cryoprotectant solutions.</P>

水 海洋係 專門大學 通信科 敎育課程 開發 硏究

金永煜,金秀植,高南泳,梁義藏,金圭煥,文建,鄭東洙 군산대학교 1984 群山水産專門大學 硏究報告 Vol.18 No.1

We have studied on the curriculum to develop the communication department of the inter-related fisheries and marine junior college groups and abridged them as follows. (1) A vessel communication has the special characteristics compared with general communication field and also it has an aim to protect the lives and the property from dangerous navigation. For these reasons we must educate many competent graduates who are versed in business and accustom themselves to necessary knowledge of communication and can be adapted them selves to the advanced communication method, techniques and international information on the communication. (2) Training goal and way of thinking of students were rearranged and the occupational cluster in the electronic-communication field was fractionated to develop the curriculum. Especially regarding operator's duty served the above purpose as a occupational cluster, we analyzed their job in accordance with it. (3) International convention on standards of training certification and watchkeeping for seafarers, 1978 was reflected, also international telecommunication convention was referred so that it might be satisfied with the condition to be observed. (4) Developing the curriculum, we responed and analyzed the questions from scientific world, industrial organizations and graduates and provide their advice and suggestions form consultive committee and reflected them to develop the curriculum

국내 다기관에서 조사한 지역사회획득 메티실린내성 황색포도알균의 빈도와 임상적 특성

송진수,최평균,송경호,조재현,김성한,방지환,이창섭,박경화,박경운,신수,최희정,김의석,김동민,이미숙,박완범,김남중,오명돈,김의종,김홍빈,최강원 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

목적 : 최근 전세계적으로 지역사회획득 메티실린내성 황색포도알균(community-associated methicillin-resistant Staphylococcus aureus, CA-MRSA)의 보고가 증가하고 있다. 하지만, 우리나라에서는 CA-MRSA 감염증에 대한 증례보고만 있을 뿐 아직까지 체계적인 연구결과가 없는 실정이다. 저자들은 국내에서 CA-MRSA의 빈도, 감염증의 임상적 양상, 분리된 균주의 항균제내성 양상을 조사하였다. 재료 및 방법 : 2005년 1월부터 2005년 6월까지 7개 병원에서 MRSA가 분리된 환자의 명단을 파악한 후 의무기록지와 건강보험심사평가원의 자료를 검토하였다. 외래나 응급실에서 혹은 입원 후 72시간 이내에 균주가 분리되고 MRSA 획득과 관련된 위험인자가 없는 경우 CA-MRSA로 정의하였으며, 분리된 균주의 임상적 의미에 따라 원인병원체(pathogen), 집락화(colonizer), 미결정(undetermined)으로 분류하였다. Penicillin과 oxacillin을 제외하고 3개 이상의 다른 계열 항균제에 내성이면 다제내성으로 정의하였다. 결과 : 연구기간동안 총 3,251주의 황색포도알균이 분리되었으며, 이 중 MRSA는 1,900주(58.4%)였다. MRSA 가운데 CA-MRSA는 114주(6.0%) 였으며, 이들이 분리된 부위는 귀(62주), 비뇨기계(14주), 피부 및 연부조직(11주), 호흡기계(10주), 혈액(3주) 등이었다. CA-MRSA 균주 가운데 집락균은 22주, 원인병원체는 22주였으며, 나머지 균주에 대해서는 그 임상적 의미를 결정할 수 없었다. 항균제 감수성 검사를 시행한 73균주 중 47주(64.4%)는 다제내성이었다. CA-MRSA 감염증 22예 중 피부 및 연부조직 감염(9예)과 중이염/외이도염(9예)이 가장 흔하였다. 침습적 감염증(invasive infection)은 4명(원발성 균혈증 3예, 감염성 관절염 1예)에서 확인되었지만, CA-MRSA 감염증으로 사망한 환자는 없었다. 결론 : 병원내 감염증에서는 MRSA가 심각한 문제이지만, 아직까지 지역사회 감염증에서 CA-MRSA는 흔하지 않았다. Background : Methicillin-resistant Staphylococcus aureus (MRSA) infection has emerged in patients who do not have the established risk factors. In Korea, little is known about the epidemiology and clinical features of community-associated MRSA (CA-MRSA). Material and Methods : Clinical microbiology laboratory databases of 7 hospitals were reviewed to identify the patients from whom MRSA was isolated during the period of January to July 2005. Only one isolate per patient was enrolled. In order to identify the risk factors of MRSA acquisition, the medical records and the Health Insurance Review Agency databases were reviewed. CA-MRSA was defined as MRSA isolated from patient without established risk factors. We analyzed patient demographics, underlying medical conditions, characteristics of infection, and antimicrobial susceptibility profiles. Results : Of total 3,251 S. aureus isolates, 1900 (58.4%) were MRSAs. Of the MRSA isolates, 114 (6.0%) were CA-MRSA. Of 114 CA-MRSA isolates, 22 (19.3%) were colonizers, 22 (19.3%) were pathogens, and the clinical significance of remaining 70 (61.4%) could not be determined. Median age of the 22 patients with CA-MRSA disease was 47 years. Nine patients had skin and soft tissue infections, 9 ear infections, 3 bacteremia, 1 septic arthritis. Seven patients had underlying medical disease. None died of the CA-MRSA infections. Of the 73 isolates of CA-MRSA, 47 (64.4%) were resistant to more than 3 classes of antibiotics besides β-lactams. Conclusion : Although MRSA is highly prevalent among hospital-associated S. aureus infection, CA-MRSA infections are not common.