The Biochemical Characteristics and Ultrastructure of Artificial Accelerated Aging Seeds in Vegetable Seeds

Yong-Ha Chu,Kil-Woong Chung,Bong-Ku Kim,Jeung-Haing Oh,Je-Cheon Chae,Cheol-Ho Hwang,Seok-Dong Kim,Seong-Min Kim,Dong-Jin Lee 한국국제농업개발학회 1999 韓國國際農業開發學會誌 Vol.11 No.1

여름콩의 開花 · 成熟 및 形態的 特性變異

Yong Ha Chu(朱勇河),Jeung Haing Oh(吳正行),Je Cheon Chae(蔡濟天),Yeaul Kyu Seung(成烈圭),Beom Young Son(孫範永),Kil Woong Chung(鄭吉雄) 한국육종학회 1996 한국육종학회지 Vol.28 No.3

This experiment was conducted to select useful breeding materials among eighty lines or varieties of summer type soybeans tested in the experiment field, DanKook University, Cheonan in 1993. Fourty-four to sixty-two days were observed as the range of days from planting to flowering and the average was 53 days. The range of days from planting to maturity was 112 to 127 days and the average was 117 days. Eighty-six percentage of tested soybean materials was white color of flower and green color of hypocotyl, while the remains were purple in both. Soybean entries with ovate, long circular and circular type of leaf were 6%, 73% and 21%, respectively. Large, medium and small leaf in the leaf area were 34%, 32% and 34%, respectively. Light green leaf color was observed as 15%, and green and dark green color were 70% and 15% among tested soybeans, respectively. Soybeans with tan pods were observed as 49%, brown and dark brown color were 14% and 37%. Erect type of pubescence was 36%, whereas curly and appressed type were 54% and 10%, respectively. Sparse, normal and dense pubescence density were 16%, 41% and 43%, respectively. Most soybean entries(63%) showed gray pubescence and the remains were brown color. Days from planting to flowering(DPF) showed highly negative correlation with days from flowering to maturity(DFM) and days from planting to maturity (DPM), whereas it was highly positively correlated with leaf area. Days from flowering to maturity was highly positively associated with days from planting to maturity, while it showed highly negatively correlated with leaf area. Days from planting to maturity was highly negative correlation with leaf area.

C형 간염 간이식 환자에서의 간염 재발 및 처치

오기봉,이승규,이영주,박광민,황신,김기훈,안철수,문덕복,주종우,양현승,하태용,조성훈 대한간학회 2003 Clinical and Molecular Hepatology(대한간학회지) Vol.9 No.3

목적: 말기 간질환으로 간이식 수술을 시행받은 환자 중 원인 질환이 C형 간염인 경우는 한국에서는 아직 증례수가 적지만, 최근 빈도가 높아지고 있다. 본 연구에서는 C형 간염으로 간이식을 받은 환자들의 간이식후 이상 경과와 재발 및 치료에 대하여 알아보고자 후향적 연구를 시행하였다. 대상과 방법: 1992년 8월부터 2002년 8월까지 간이식 프로그램에서 시행된 587예의 간이식 중 C형 간염의 원인 질환이었던 16예(2.7%)를 연구 대상으로 하여 C형 간염 재발을 중심으로 본 임상 경과와 그에 다른 처지에 대하여 환자들의 의무기록과 임상자료를 분석하는 후향적 연구를 시행하였다. 결과: C형 간염으로 간이식을 받은 16예 중 성별로는 남자 11명과 여자 5명이었고, 정중 연령은 56±6세(42-62)이었고 정중 추적 기간은 6±13개월(1-41)이었다. 13예는 생체부분 간이식을 시행받았고, 3예는 뇌사자 전간이식을 받았다. 간이식후 이상적 재발이 의심되었던 경우는 16예 중 9예(56.3%)를 차지하였고, 평균 재발기간은 5.2개월이었다. 조직학적 재발은 8예(50%)이었다. HCV RNA PCR 검사상 양성 소견은 90.9%로 검사가 시행된 시기의 환자들을 대상으로 했을 때 임상적 재발 및 조직학적 재발군 모두에서 양성이었다. 조직학적 재발이 관찰된 8예 중 5예에서 리바비린 단독투여를, 2예는 인터페론 및 리바비린 병합투여를 시행하였다. 치료 후 경과상 혈청 aminotransferase치가 정상화되거나 거의 정상범위로 호전된 경우는 6예(75%)이었다. 결론: 본 연구를 통해 C형 간염으로 간이식 수술을 시행받은 환자들에서 C형 간염의 재발률은 높았으며, 이는 외국의 경우와 유사한 결과를 보여주었다. 그래서 C형 간염의 재발 및 재발이후 간경변으로의 진행을 막는 것은 환자 및 이식 장기의 생존을 위하여 중요하며, C형 간염의 재발에 대한 적절한 protocol의 개발이 필요하다. Background/Aims: End-stage liver disease caused by viral hepatitis C has been increasing recently in Korea. In this study, we investigated the clinical progress, recurrence, and management of hepatitis C patients who underwent liver transplantation. Methods: We retrospectively reviewed the clinical progress and management of 16 patients (2.7%) with hepatitis C among 587 liver transplant patients from August 1992 to August 2002. Results: Eleven cases among 16 patients were males. The median age was 56±6 (42-62) years and the median follow-up period was 6±13 (1-41) months. Thirteen cases underwent living donor liver transplantation and three had cadaveric whole liver transplantation. Clinical recurrence occurred in nine cases (56.3%) and mean time of recurrence was 5.2 months. Histological recurrence cases were eight (50%). A positive result of HCV RNA PCR was found in 90.9%, and all cases of clinical and histological recurrence in groups in the same periods were PCR-positive. among eight cases showing histological recurrence, five patients were managed by ribavirin monotherapy, two patients received interferon and ribavirin combination therapy, and one patient was not treated at all. The serum aminotransferase level was normalized in six cases (75%) of them. Conclusions: We observed that the HCV reinfection rate of a transplanted liver was high in this study, as in other reports in the literature. The prevention of HCV recurrence and the management of post-recurrent cirrhotic change are crucial for graft and patient survival. We think customized protocols are needed for every situation of recurrent hepatitis C.(Korean J Hepatol 2003;9:180-187)

[P211] Low infectivity of scabies in tertiary well controlled health care center

( Yeongjoo Oh ),( Howard Chu ),( Eunjin Ha ),( Kee Yang Chung ),( Chang Ook Park ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1

Scabies had been considered to be highly infective and need instant isolation after diagnosis. However, even though scabies is found time to time, true contagious infection is found much rarely in real tertiary health care center with clean environment. On August 2016, 73 years old female patient, stayed long time in nursing center, admitted into intensive care unit (ICU) and after 2 weeks of care, she was transferred to general ward (GW). After 3 weeks of care in GW the scabies was found from the patient. Retrospective contact history was taken and we revealed that total 27 patients with 52 health care workers had contacted in ICU and total 10 patients with 30 health care workers had contacted in GW. 2 months of follow-up was done for whole people and only 1 health care worker, who was nursing person of the other patient in GW, showed scabies after 1 month. In ICU every health care workers use disposable gloves and gown when contact each patient and the infectivity of scabies was 0 among 79 people for 2weeks of exposure. In GW the infectivity was 1 among 40 people for 3 weeks of exposure. Even for 1 infected person, the infection in our hospital was not certain because she had worked in nursing center a month ago. From this result, we found out that the real infectivity was extremely low in clean well-controlled environment.

Quench Detection Based on Voltage Measurement for the KSTAR Superconducting Coils

Yong Chu,Yonekawa, H.,Kim, Y.O.,Park, K.R.,Hyun Jung Lee,Park, M.K.,Park, Y.M.,Lee, S.J.,Ha, T.H.,Oh, Y.K.,Bak, J.S. IEEE 2009 IEEE transactions on applied superconductivity Vol.19 No.3

<P>To protect the KSTAR (Korea Superconducting Tokamak Advanced Research) superconducting coils against a quench, the quench detection system based on voltage measurement was fabricated. It used a detect/dump scheme which detects the presence of non-recovering normal zones and activates a dump circuit that transfers most of the stored energy into a dump resistor. Even though it is desirable to discharge the coil energy as fast as possible after quench detection, a time delay between the quench detection and the complete dump circuit actuation will be necessary in order that the quench voltage is to be distinguished from various noises. The quench threshold voltage and the delay time should be set before operation for quench voltage detection so that the maximum hot-spot temperature could be limited to 150 K. This paper describes the hardware techniques to prevent the malfunction of the quench detection due to voltage noises arising from the KSTAR operating scenarios. During the first operation of the KSTAR machine, the inductive voltages and other voltage noises were measured and effectively compensated below voltage thresholds. A quench did not occur and the quench detection system was well operated without any false activation.</P>

코팅제를 이용한 인공경량골재의 흡수율 제어에 관한 연구

오다영(Oh, Da Young),최지선(Choi, Ji Sun),최훈제(Choi, Hoon Jae),이근주(Lee, Kewn Chu),하주형(Ha, Ju Hyung),조윤구(Cho, Yun Gu) 한국콘크리트학회 2016 한국콘크리트학회 학술대회 논문집 Vol.28 No.1

Decadal modulation of Pacific Decadal Oscillation on the relationship between ENSO and western North Pacific high

Jung-Eun Chu,Hyoeun Oh,Seon-Hwa Kim,Kyung-Ja Ha 한국기상학회 2013 한국기상학회 학술대회 논문집 Vol.2013 No.4

채소종자의 인위 노화처리에 따른 생화학적 특성과 표면미세구조의 변화

이동진,오정행,황철호,채제천,김석동,김성민,김봉구,정길웅,주용하 한국국제농업개발학회 1999 韓國國際農業開發學會誌 Vol.11 No.1

본 실험에서는 저활력 및 노화종자의 체계적 선별을 위하여 종자를 인위적으로 노화시켜 노화단계별 종자의 활력저하정도, sinapine 누출정도, 유기·무기성분, 지방산조성, 저장단백질의 SDS-PAGE를 이용한 band pattern 변화 등 노화종자의 생리·생화학적 특성을 조사하여 노화 종자에 대한 기초자료를 얻기 위하여 수행한 결과는 다음과 같다. 1. 배추종자는 3일간의 노화처리시 47.3%의 발아율을 보였으나 4일간 처리시에는 전혀 발아하지 않았으며, 무·고추에 비해 노화속도가 빨랐다. 2. 배추의 노화종자와 건전종자는 침지 후 시간이 경과할수록 sinapine 누출액이 증가하였으며, 노화종자는 건전종자에 비하여 그 증가폭이 현저히 컸다. 3. 노화처리한 배추종자는 건전종자에 비하여 아미노산 및 K의 누출량은 현저히 증가하였으나 Ca·Mg·Na 등은 큰 차이가 없었다. 배추의 노화종자는 침지시간이 경과할수록 당의 누출량이 증가하였으며, 누출된 유리당은 glucose, fructose 및 sucrose였다. 4. 배추종자의 노화는 포화 및 불포화지방산의 전체함량에 큰 영향은 없었으며, 무는 차이를 보이지 않았다. 고추의 노화종자는 건전종자와 비교하여 포화지방산의 전체 함량은 증가한 반면, 불포화지방산의 전체 함량은 감소하였다. 5. 배추 및 무의 SDS-PAGE법에 의한 저장단백질의 band pattern에서는 노화될수록 band가 흐려지거나 나타나지 않았다. 6. 노화된 배추종자의 종피와 자엽표면에서 심한 균열 및 조직의 파괴가 전자현미경적으로 관찰되었다. These experiments were conducted to investigate the biochemical and morphological characteristics of aged and non-aged seeds for increasing seed viability. Optimum duration of artificial aging treatment to test aged seeds was observed; 4 days for Chinese cabbage; and 5 days for radish seed. Sinapine leakage in Chinese cabbage and radish were increased with increased treatment duration from 1hr to 8hrs. Aged seeds had higher sinapine leakage after soaking in water than that of the non-aged seeds. Aged seeds of Chinese cabbage had bigger leakage in amino acid and potassium content than non-aged seeds. For total sugar content of Chinese cabbage, aged seeds were increased as soaking time also increased, while non-aged seeds were not. The kind of leaked sugar from aged seeds consist of glucose, fructose and sucrose for Chinese cabbage. Cell ultrastructure of aged seed surface in Chinese cabbage was observed irregularly cracked and breakdown with holes in cotyledon surface, while surface tissue of non-aged seed was well arranged and compacted. Aged red pepper seed had higher content of saturated fatty acid (palmitic acid), but lower content of unsaturated fatty acid (oleic acid and linoleic acid) than that of non-aged seeds. Banding patterns of seed storage protein using SDS-PAGE in aged seeds of Chinese cabbage and radish were characterized by the disappearance of major band as compared to normal seed of crops.

Quenching of Ofloxacin and Flumequine Fluorescence by Divalent Transition Metal Cations

Park, Hyoung-Ryun,Oh, Chu-Ha,Lee, Hyeong-Chul,Choi, Jae-Gyu,Jung, Beung-In,Bark, Ki-Min Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.12

This study examined the quenching of ofloxacin (OFL) and flumequine (FLU) fluorescence by $Cuj^{2+}$, $Ni^{2+}$, $Co^{2+}$ and $Mn^{2+}$ in an aqueous solution. The change in the fluorescence intensity and lifetime was measured at various temperatures as a function of the quencher concentration. According to the Stern-Volmer plots, the fluorescence emission was quenched by both collisions (dynamic quenching) and complex formation (static quenching) with the same quencher but the effect of static quenching was larger than that of dynamic quenching. Large static and dynamic quenching constants for both OFL and FLU support significant ion-dipole and orbital-orbital interactions between fluorophore and quencher. For both molecules, the static and dynamic quenching constants by $Cu^{2+}$ were the largest among all the metal quenchers examined in this study. In addition, both the static and dynamic quenching mechanisms by $Cu^{2+}$ were somewhat different from the quenching caused by other metals. Between $Ni^{2+}$ and FLU, a different form of chemical interaction was observed compared with the interaction by other metals. The change in the absorption spectra as a result of the addition of a quencher provided information on static quenching. With all these metals, the static quenching constant of FLU was larger than those of OFL. The fluorescence of OFL was quite insensitive to both the dynamic and static quenching compared with FLU. This property of OFL can be explained by the twisted intramolecular charge transfer in the excited state.

Quenching of Ofloxacin and Flumequine Fluorescence by Divalent Transition Metal Cations

Hyoung-Ryun Park,Chu-Ha Oh,Hyeong-Chul Lee,Jae Gyu Choi,Beung-In Jung,Ki-Min Bark* 대한화학회 2006 Bulletin of the Korean Chemical Society Vol.27 No.12

This study examined the quenching of ofloxacin (OFL) and flumequine (FLU) fluorescence by Cu2+, Ni2+, Co2+ and Mn2+ in an aqueous solution. The change in the fluorescence intensity and lifetime was measured at various temperatures as a function of the quencher concentration. According to the Stern-Volmer plots, the fluorescence emission was quenched by both collisions (dynamic quenching) and complex formation (static quenching) with the same quencher but the effect of static quenching was larger than that of dynamic quenching. Large static and dynamic quenching constants for both OFL and FLU support significant ion-dipole and orbital-orbital interactions between fluorophore and quencher. For both molecules, the static and dynamic quenching constants by Cu2+ were the largest among all the metal quenchers examined in this study. In addition, both the static and dynamic quenching mechanisms by Cu2+ were somewhat different from the quenching caused by other metals. Between Ni2+ and FLU, a different form of chemical interaction was observed compared with the interaction by other metals. The change in the absorption spectra as a result of the addition of a quencher provided information on static quenching. With all these metals, the static quenching constant of FLU was larger than those of OFL. The fluorescence of OFL was quite insensitive to both the dynamic and static quenching compared with FLU. This property of OFL can be explained by the twisted intramolecular charge transfer in the excited state.