EFFECTS OF LEAF MATURITY ON THE DISEASE PROGRESS OF SEPTORIA BROWN SPOT IN SOYBEAN

오정행,Oh Jeung Haing The Korean Society of Plant Pathology 1987 Plant Pathology Journal Vol.3 No.4

대두 갈색무늬병의 진전은 한 식물체에서 상위엽으로의 진전속도(수직감염율 : vertical progress)과 밀접한 관계가 있다. 실제로 포장에서의 갈색무늬병 진전은 주로 하위엽에서 발병하기 시작하여 상위엽에 비하여 이병정도가 높은 것이 보통이다. 이러한 현상의 원인을 구명하기 위하여 수행한 본 실험에서 갈색무늬병의 감염율은 잎의 성숙도와 정의 상관을 보였으며 이러한 유엽저항성은 접종원의 분포와는 무관한 것으로 보였다. 또 상위엽의 엽표면일출액 (leaf diffusate)은 병원균 포자발아 및 관계신장의 억제 정도가 하위엽의 일출액에 비하여 높았으며 이러한 경향은 저항성품종에서 더욱 현저하여 엽일출액의 어떤 발아억제물질이 갈색무늬병의 유엽저항성에 관여하는 것으로 추정되었다. Septoria brown spot caused by Septoria glycines Hemmi is one of the serious fungal diseases in soybean. Since little has been known about the disease progress in the field, the present study was conducted to determine the factors affecting the disease progress in the soybean plant. Disease severity and pattern of the progress of the Septoria brown spot were different with varieties. Susceptibility of soybean plants increased with increase of plant age and leaf maturity in order from the primary leaf to the newly expanded leaf. It seemed to be related with conidial germination on the leaves. Germination and germtube elongation were more inhibited by the diffusates obtained from upper leaves than those from lower leaves and they were higher in a susceptible variety than in a moderately resistant one.

포도 흰가루병균(Uncinula necator)의 분생포자 형성과 발아에 미치는 온도, 습도, pH 및 Trizole 살균제의 영향

오정행,Oh, Jeung-Haing 한국식물병리학회 1997 Plant Pathology Journal Vol.13 No.4

포도 흰가루병균(Uncinula necutor)의 분생포자 형성, 발아 및 균사 초기생장에 미치는 중요 환경오인과 살균제의 영향을 구명하기 위하여 수행한 연구 결과를 요약하면 다음과같다. 분생포자 형성은 상대습도 76~96%에서 가장 좋았다. 포자 발아 및 균사 초기생장은 증류수에 비해 5% 한천배지에서 좋았으며, 온도 $26^{\circ}C$, pH 5.0일 때 가장 좋았다. 광 100 lux는 암상태와 발아율에 차이가 없었으며 한천배지에 포도 엽즙액 30%를 첨가한 배지에서는 순수 한천배지 보다 좋았다. 또 triazole계 살균제 첨가 배지에서의 포자발아 및 균사생장은 살균제 농도증가에 비례하여 급격히 감소하였으나 그 정도는 살균제의 종류에 따라 차이가 있었다. 특히, myclobutanil은 농도증가에 따른 발아율의 감소정도가 매우 낮았다. The experiment was conducted to obtain basic informations on the effects of key environments and fungicides on the sporulation, conidial germination and hitial growth of the hyphae of grape powdery mildew fungus Uncinula necator. Maximum sporulation occurred at RH 75~96% in vitro adiusted with sulfuric acid solution. Conidial germination and initial growth of the hyphae were better at 5% water agar than distilled water, and best at $26^{\circ}C$ and pH 5.0 of the substrates. Germination occurred equally well at light intensity of below 100 lux or dark condition, which was better at water agar supplemented with leaf extract 30% than pure water agar. The water agar supplemented with triazole fungicides reduced conidial germinations and initial growth of the hyphae signifcantly, in proportion to the increase in concentration of the fungicides, but the maguitude of reduction depended on the fungicides. Particularly in myclobutanil, reduction rate was very low as increased in concentration.

녹두 갈색무늬병균(Cercospora canescens Ellis & Martin)의 분생포자 형성에 관한 연구

권신한,오정행,Kwon Shin Han,Oh Jeung Haing 한국응용곤충학회 1981 한국식물보호학회지 Vol.20 No.1

녹두 돌연변이계통의 갈색무늬병에 대한 저항성검정을 위하여 접종실험에 필요한 다량의 분생포자를 얻을 수 있는 배양조건 실험결과를 요약하면 다음과 같다. 1. 갈색무늬병균의 분생포자 형성에 적합한 배지는 기주식물조직배지인 녹두엽즙배지였으며 약 2500Lux의 형광조사는 분생포자 형성을 촉진시켰다. 2. 배양기상의 기중균사 거는 분생포자 형성을 현저히 증가시켜 OA배지에서도 MOA에서와 같은 량의 많은 분생포자 형성이 가능하였다. 3. 포자형성의 최적조건은 색소생성 및 균사장의 최적조건과 일치하지 않는 것으로 보였다. This study was conducted to obtain a supply of conidia sufficient for screening mungbean mutant lines for a source of resistance to Cercospora leaf spot caused by Cercospora canescens Ellis and Martin. Abundant sporulation occurred in cultures on mungbean leaf decoction oatmeal agar(MOA) exposed to about 2,500 Lux of fluorescent light. but it did not occur in continuous darkness. The conditions that produced maximum number of conidia was not coincided with those for vegetative growth and pigmentation in culture medium. Removal of aerial mycelium in culture by brushing with sterile water so enhanced the conidial production that oatmeal agar medium(OA) could be useful for production of abundant conidia by the treatment.

여름콩의 開花 · 成熟 및 形態的 特性變異

Yong Ha Chu(朱勇河),Jeung Haing Oh(吳正行),Je Cheon Chae(蔡濟天),Yeaul Kyu Seung(成烈圭),Beom Young Son(孫範永),Kil Woong Chung(鄭吉雄) 한국육종학회 1996 한국육종학회지 Vol.28 No.3

This experiment was conducted to select useful breeding materials among eighty lines or varieties of summer type soybeans tested in the experiment field, DanKook University, Cheonan in 1993. Fourty-four to sixty-two days were observed as the range of days from planting to flowering and the average was 53 days. The range of days from planting to maturity was 112 to 127 days and the average was 117 days. Eighty-six percentage of tested soybean materials was white color of flower and green color of hypocotyl, while the remains were purple in both. Soybean entries with ovate, long circular and circular type of leaf were 6%, 73% and 21%, respectively. Large, medium and small leaf in the leaf area were 34%, 32% and 34%, respectively. Light green leaf color was observed as 15%, and green and dark green color were 70% and 15% among tested soybeans, respectively. Soybeans with tan pods were observed as 49%, brown and dark brown color were 14% and 37%. Erect type of pubescence was 36%, whereas curly and appressed type were 54% and 10%, respectively. Sparse, normal and dense pubescence density were 16%, 41% and 43%, respectively. Most soybean entries(63%) showed gray pubescence and the remains were brown color. Days from planting to flowering(DPF) showed highly negative correlation with days from flowering to maturity(DFM) and days from planting to maturity (DPM), whereas it was highly positively correlated with leaf area. Days from flowering to maturity was highly positively associated with days from planting to maturity, while it showed highly negatively correlated with leaf area. Days from planting to maturity was highly negative correlation with leaf area.

면역이중확산법에 의한 콩 종자의 모자이크 바이러스(SMV) 감염상 조사

나용준,박원철,오정행,La Yong-Joon,Bak Won-Chull,Oh Jeung-Haing 한국응용곤충학회 1983 한국식물보호학회지 Vol.22 No.1

우리 나라 콩 종자의 모자이크 바이러스(Soybean mosaic virus; SMV) 감염상을 조사하기 위하여 국내에서 수집한 8개 품종을 공시해서 Lima와 Purcifull의 면역이중확산법으로 SMV 검정을 한 결과는 다음과 같다. 1. 공시한 7개 품종 중 6개 품종에서 SMV가 검출됨으로서, 우리 나라의 콩 품종에서 SMV의 종자전염성이 혈청학적 방법으로 확인되었다. SMV가 검출된 종자의 종자감염성율은 최저 $2.1\%$에서 최고 $12.5\%$를 나타냈으며, 전체적으로는 총검정립수 336립 중 18립에서 SMV가 검출됨으로서 약 $5.4\%$의 감염율을 보였다. 2. 이병주에서 채집한 갈반립과 무갈반립수의 SMV 감염율은 북해 1호 품종의 경우 각각 $33.3\%$와 $29.2\%$ Clark 품종의 경우 각각 $4.2\%$와 $0.0\%$, Woodworth 품종의 경우는 갈반립과 무갈반립 모두 $4.2\%$의 감염율을 나타냄으로써, 공시한 콩 품종의 갈반립과 무갈반립간엔 SMV 감염율에서 뚜렷한 차이를 발견할 수 없었다. 3. SMV에 감염된 광교품종 중 괴저병징을 나타내는 개체에서 채종한 종자에서는 전혀 SMV가 검출되지 않아 광교품종에서는 SMV-N가 종자전염되지 않는 것으로 보였다. Soybean mosaic virus (SMV) infection in seeds wits investigated by an immunochemical method Seven soybean cultivars or lines Bughae No.1, KAS 662-7, Chungbugbaeg, Gwanggyo Clark, Bongeui, and Gangrim were tested using hypocotyls of germinated seeds and presence of SMV was detected in six soybean cultivars but Gangrim. The level of SMV infection in the assayed cultivars varied from 2.1 to $12.5\%$. It seemed that seed coat mottling had no correlation with seeds. SMV infection of the seeds since virus has not always been detected from the mot tled seeds SMV has not been detected in the seeds of variety Gwanggyo which showed necrotic symptoms.

γ-線처리 감자 節莖 器內培養에서 誘起된 突然變異體의 疫病抵抗性 反應과 RAPD 분석

Jae Sung Kim(金在成),In Chui Shin(申仁撤),Jeung Haing Oh(吳正行),Young Sun Park(朴英善),Yong Hwan Lee(李龍煥) 한국육종학회 1996 한국육종학회지 Vol.28 No.3

For the purpose of mutation induction and selection of the potato variants from in vitro culture of nodal stem by the Υ-ray irradiation, their reaction to the late blight of potato(Phytophthora infestans) and RAPD analysis were conducted with the potato cultivars Jopung, Dejima and Superior. 1. Incidene of variations such as chlorophyll deficiency, leaf color change, adventitious root, microtuber formation was found in the nodal stem cultures. The induction rates of the morphological mutants were ranged from 19.3 % to 30.9% in 30Gy irradiation of Υ-ray as compared with 7.2% in the nonirradiated culture. 2. The plants at the 1st clonal generation originated from nodal stem culture showed very poor growth in the field. The plants derived from Υ-ray irradiated nodal stem culture revealed the marked reduction in plant growth than that of nonirradiated one. 3. Incidence rates of patato variants such as dwarf type, chlorophyll deficiency, change of leaf colors and tuber types at the 2nd clonal generation were 4.5% in nonirradiated group but 30.3% in irradiated group. The incidence rate of morphological mutants was 6.9% in Dejima, 3.4% in Jopung, 3.3% in Superior under the nonirradiated condition, while it was 31.0% in Superior, 30.7% in Jopung, 28.6% in Dejima under the irradiated condition. 4. The reaction of the potato variants selected from the 2nd clonal generation against late blight disease was different according to the original cultivars. The percent of diseased leaf area of selected potato variants from the Υ-irradiated population was significantly lower with the range of 18.8~41.3% according to the original cultivars as compared with those selected from the nonirradiated population. This suggested that there are some possiblity to select potato variants resistant to late blight by Υ-ray irradiation under in vitro culture condition. 5. In RAPD analysis of the potato plant originated from the 2nd clonal generation, five among twelve tested primers showed a good amplification and polymorphisms in twelve among 73 bands. The analysis sugguested that the homogenity between Dejima and Superior was closer than that between Superior and Jopung. The selected resistant potato variants exhibited considerably different banding patterns in two primers.

보리의 稈長 및 强稈性 遺傳

Bong Ku Kim(金鳳九),Chang Hwan Cho(曺章煥),Jeung Haing Oh(吳正行),Kil Wong Chung(鄭吉雄),Je Cheon Chae(蔡濟天),Ouk Kyu Han(韓玉圭) 한국육종학회 1995 한국육종학회지 Vol.27 No.2

This study was conducted to investigate the inheritance of culm length and straw stiffness, and to observe the relationship between culm length and other agronomic characteristics in barley. Triple bearded club mariout (C. I. 2523) having the characters of short culm length and straw weakness was crossed with three barley cultivars, Gangbori, Olbori and Chalbori having long culm length and straw stiffness. Culm length, straw stiffness and agronomic characters in parents, F₁ and F₂ were investigated the in field. All three barley crosses, C. I. 2523/Gangbori, C. I. 2523/Olbibori and C. I. 2523/Chalbori, showed good fitness to F₂ segregation ratio of 3 long : 1 short in culm length. The short culm length of C. I. 2523 may be controlled by single recessive gene. Culm length showed positive phenotypic and genotypic correlations with each of 3rd and 4th internodes. Culm length was significantly associated with the leaf blade length but not with the leaf blade width. The negative phenotypic and genotypic correlations were observed between culm length and heading date in all three barley F₂ populations. Culm length also revealed significantly positive phenotypic and genotypic correlations with number of grains per spike, grain weight per spike, number of spike per plant, 1,000-grain weight and yield per plant. F₂ analysis suggested that Gangbori carries one recessive gene associated with straw stiffness and chalbori may carry one dominant gene. C. I. 2523/Olbori F₂ showed the segregation ratio of 13 stiff : 3 weak in stiffness of straw, implying the genetic interaction between alleles. Culm length showed highly positive phenotypic and genotypic correlations with the outside diameter of culm and the weight of culm breaking.

채소연작 비닐하우스내에서 조숙풋콩의 재배가 토양화학성분, 생육 및 수량형질에 미치는 영향

오정행,주문갑,정구복,정길웅,주용하,고상덕 한국콩연구회 1996 韓國콩硏究會誌 Vol.13 No.2

This experiment was conducted to investigate any change in chemical properties of soil as well as growth and yield components of Seokryangputkong cultivated is vinyl house mainly used for consecutive cultivations of vegetables. Seokryangputkong, a recommended variety as a vegetable soybean by Rural Development Administration in 1995, was planted 4 times from April 15 to May 15 in 10 days - interval in vinyl house in Such'ang-ri, Choch'iwon-up, Yunki-gun, Chungnam, the analysis of the chemical properties of the soil indicated that available P₂O₂ content, EC, organic matter content and Ex-Na were increased by 89.3mg/kg, 0.1dS/m, 0.1% and 0.1me/100g, after a cultivation of vegetable soybean, respectively. Whereas, Ex-Ca and Fx-Mg were decreased by 1.2me/100g and 0.1me/100g, respectively and pH and Ex-K were not changed. Emergence date, days to emergence and average emergence period were shortened as planting delayed in 10 days - interval from April 15. There was no difference shown in emergence rate in different planting dates. Days to flowering, pod formation and maturity were respectively shortened by 8.3 days, 2.7 days and 11.4 days as planting dates delayed in 10 days - interval from April 15. Days to flowering was not significantly correlated with days to pod formation but it showed a highly positive correlation with days to maturity. Days to pod formation showed a highly positive correlation with days to maturity, too. Stem height, stem diameter, number of stem nodes, number of branches, number of branch nodes and internode length of cotyledon were reduced as planting dates delayed, and fresh weight per plant did not show any difference as planting dates delayed. The number of unfilled and one - seed pods per plant were reduced as planting dates delayed, and number of two - seed and three - seed pods per plant, number of pods per plant and fresh weight per plant did not show any difference as planting dates delayed. Pod length and pod width showed some difference in different planting dates but there was no difference in pod thickness. The number of seeds per plant, seed weight per plant, one hundred - seeds weight, seed length and seed width were not different among planting date. Seed thickness was reduced as planting dates delayed. Among the various characteristic, stem diameter showed a highly positive correlation with seed thickness. The number of branches and branch nodes showed a highly positive correlation with one hundred - seeds weight and seed thickness. The number of pods per plant showed a highly positive correlation with fresh weight per plant and pod length. the number of one-seed per plant showed a highly positive correlation with fresh weight per plant, fresh weight of pods per plant and weight of seeds per plant Fresh weight of pods per plant showed a highly positive correlation with weight of, seeds per plant One hundred - seeds weight showed a highly positive correlation with seed width and seed length showed a highly positive correlation with seed width. The other characteristics did not show any significant correlation reciprocally.

마늘 잎마름 症狀의 藥劑防除 效果

吳正行 단국대학교 1994 論文集 Vol.28 No.-

To obtain a basic information necessary for controlling leaf blight caused by complex infection with Stemphyllium botryosum and Alternaria porri in gralic, fungicides, Capro WP, Ipro WP and Metasyldong WP were examined for the inhibition activity of growth and control value to the pathogens. The result obtained were summarized as follows. 1. Leaf blight of gralic caused by the complex infection with S. botryosum and A. porri occured at the beginning of May and drastically increased at the beginning of July when the aged plants were subjected to high temperature and mositure short of fertilizer. 2. Mycelial growth of S. botryosum and A. porri was more inhibited by Capro WP and Ipro WP than Metasyldong WP and it was marked even at the concentration of 1㎍/ml which is used for field spary in general. S. botryosum was more inhibited than A. porri. 3. Sporulation of S. botryosum and A. porri was markedly reduced by the fungicides, even at the concentration of 1㎍/ml. There was not significant in difference between the fungicides but the reduction was greater in S. botryosum than A. porri. 4. Control value of the fungicides was 75.0% in Metasyldong WP, 80.1% in Capro Wp and 80.6% in Ipro WP. The value increased positively in proportion to inhibition of mycelial growth and spore formation. 5. It could be concluded that the fungicides tested might be not sufficient for controlling leaf blight of gralic caused by complex infection with S. botryosum and A. porri, because A. porri was less sensitive to the fungicides.

녹두 수집 유전자원의 형태적 특성 및 분류

이동진,오정행,봉현성 한국국제농업개발학회 1998 韓國國際農業開發學會誌 Vol.10 No.4

녹두의 품종육성을 위한 기초자료를 얻고자 국내수집 유전자원 96점을 공시하여 형태적 형질변이, 종실특성, 형질들간의 상관관계 등을 조사하여 계통군을 분류한 결과는 다음과 같다. 1. 수집된 녹두 유전자원의 지역적 분포를 보면 경기 13점, 강원 6점, 충북 1점, 전북 25점, 전남 16점, 경북 21점 및 경남 14점 등 96점이었다. 2. 공시 유전자원의 질적형질로서 배축색은 자색이 88.5%, 염색은 담록색이 41.7% 엽 병색은 녹색이 86.5%, 화색은 자색이 88.5%, 성숙협색은 흑색이 71.9% 및 종피색은 녹색이 93.8%를 차지하였다. 3. 파종부터 개화기까지의 일수는 54∼69일로 평균 60.4일이었으며, 성숙시까지의 일수도 81∼102일로 다양하게 분포하고 있었으며 평균 89.9일 이었다. 4. 주당 협수는 2∼15개 범위로 분포하였으며, 협당립수 8∼16개 및 100립중은 1.8∼5.4g 범위로 다양하였다. 5. 갈색무늬병의 발병도는 10∼85% 범위로 평균 52.7%로 나타났으며, 이들의 변이폭이 매우 커서 병저항성 계통육성을 위해 유용하게 이용 할 수 있을 것으로 보였다. 6. 주당분지수는 개화까지의 소요일수와 밀접한 상관관계를 나타냈으며, 백립중은 협당립수, 협장, 협폭, 입폭 등과 고도로 높은 정상관을 보였다. 7. 공시한 96점의 유전적 유연 관계를 분석한 결과 4개 집단으로 분류 할 수 있었다. Ⅰ 그룹은 생육 일수가 다소 길며, 협장이 짧고, 입장 및 입폭이 작으며, 소립종의 특성을 나타냈다. Ⅱ 그룹은 분지수가 많고 개화기까지의 일수가 비교적 짧은 특성을 보였다. Ⅲ 그룹은 백립중이 무거웠으며, 입장, 입폭, 협장 및 협폭이 컸다. Ⅳ 그룹은 개화시까지의 생육일수는 60일 정도였으며, 100립 중은 3.32g 및 종실 특성은 Ⅱ 그룹과 비슷하였다. This experiments were conducted to characterize collected mungbean germplasm and to obtain basic information for breeding of mungbean. Ninety six accessions from Korea were used in this study. Regional distribution of tested accessions were 13 accessions from Kyonggi, 6 accessions from Kangwon, 1 accession from Chungbuk, 25 accessions from Chonbuk, 16 accessions from Chonnam, 21 accessions from Kyongbuk and 14 accessions from Kyongnam, respectively. In the characterization of qualitative characters, purple in the hypocotyl color of tested germplasm was 88.5%, 41.7% for light green in leaf color, 86.5% for green in petiole color, 88.5% for purple in flower color, 71.9% for black in pod color and 93.8% for green in seed coat color, respectively. Days from sowing to flowering and maturity ranged from 69-74 and 81-102 days, and average was 60.4 and 89.9 days, respectively. The number of pods per plant ranged from 2 to 15 with an average of 8 and number of seeds per pod ranged from 9∼16 with an average of 12. The weight of one hundred seeds ranged from 1.8 to 5.4g and its average was 3.6g. Cercospora leaf spot(CLS) disease ranged from 10∼85% with an average 52.7%. The high variation in mungbean germplasms was suggested that it is very useful as gene sources for breeding materials of CLS resistance. The number of branches per plant was closely correlated with the days from sowing to flowering, and 100 grains weight was significantly correlated with the number of seeds per pod, pod length, pod width and grain width. In the cluster analysis, 96 accessions were classified into four groups. Group Ⅰ was characterized as longer in growth period, shorter in pod length, grain length and grain width, and smaller in 100 grain weight than other three groups. Group Ⅱ was characterized with high number of branches per plant and short growth periods from sowing to flowering. Group Ⅲ had longer grain length, grain width, pod length and pod width, and heavier 100 grain weight as compared to other groups. Group Ⅳ exhibited 60 days in growth period from sowing to flowering and 3.32g in 100 grain weight, and grain characteristics similar with Group Ⅱ.