미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자에서 혈장 Endothelin의 변화

서예경,정상원,남직화,신병호,전종민,김정국,김동희,하승우,김보완 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.3

목적 : 혈관내피세포에서 생성되는 endothelin(ET)는 강력한 혈관수축작용을 가진 펩타이드로 당뇨병 환자에서 혈관내피세포의 손상여부에 따라 혈중 농도가 증가할 수 있으며 혈관합병증과 관련이 있다는 보고가 있다. 그러나. 혈중 ET치의 상승과 당뇨병성 합병증의 상관성에 대해서는 논란이 많다. 이에 저자들은 인슐린 비의존성 당뇨병 환자에서 미세알부민뇨의 동반유무에 따른 혈중 ET치의 변화 및 혈장 ET치와 요중 알부민 배설률과의 상관성을 검토하였다. 대상 및 방법 : 인슐린 비의존성 당뇨병 환자중 미세알부민뇨를 보인 22명과 정상알부민뇨를 보인 43명을 대상으로 하였으며 혈장 ET를 측정하여 요중 알부민 배설률, 당뇨병성 망막증 및 당뇨병 관련인자들과의 관계를 비교하였다. 결과 : 당뇨병성 망막증이 합병된 군에서 망막증이 합병되지 않은 군에 비하여 혈장 ET치가 유의하게 높았던 반면 (각각 1.9±0.66, 1.5±0.76pg/㎖, p<0.05) 미세알부민뇨를 보인 군과 정상알부민뇨를 보인 군사이에 혈장 ET치는 통계학적으로 유의한 차이가 없었다(각각 1.9±1.14, 1.7±0.69pg/㎖). 또한 요중 알부민 배설률과 혈장 ET치 사이에 유의한 상관관계는 없었으며 미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자군에서 혈장 ET치는 당뇨병의 관련인자들과도 유의한 상관관계가 없었다. 결론 : 인슐린 비의존 당뇨병 환자에서 혈장 ET치가 초기 당뇨병성 신증의 합병여부나 미세알부민뇨의 정도를 반영하기는 어려울 것임을 시사한다. Background : Endothelin(ET), a potent vasoconstritor peptide released from vascular endothelial cells, is known to be increased in diabetic patients with vascular damage and related with diabetic angiopathy. However, whether plasma ET levels may be related to diabetic complications is controversial. We studied the change of ET levels in non-insulin dependent diabetics with microalbuminuria and relation between ET and urinary albumin excretion rate. Methods : Plasma ET levels of 22 non-insulin dependent diabetics with microalbuminuria and 43 patients with normoalbuminuria were measured and relations of plasma ET to diabetic complications such as urinary albumin excretion rate, retinopathy and other associated factors were studied. Results : Plasma ET levels were significantly higher in patients with diabetic retinopathy than those without retinopathy(1.9 ± 0.66 vs 1.5 ± 0.76 pg/㎖. p<0.05). The difference, however, between diabetics with and without microalbuminuria was not significant(1.9 ± 1.14 vs 1.7 ± 0.69 pg/㎖). There were no correlations of plasma ET to urinary albumin excretion rate and to the other associated factors in non-insulin dependent diabetics. Conclusion : These results suggest that plasma ET levels may not seem to act as a predictor of diabetic nephropathy or microalbuminuria in non-insulin dependent diabetics.

Implementation of Navigation System Available for Marine Leisure Ship

Byoung-Guk Min,Hee-ja Jeong,Tae-Jin Ha,Joon-Seub Cha,Nam-Ho Kim 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.3

Validation of One-Step Real-Time RT-PCR Assay in Combination with Automated RNA Extraction for Rapid Detection and Quantitation of Hepatitis C Virus RNA for Routine Testing in Clinical Specimens

( Byoung Guk Kim ),( Hye Sung Jeong ),( Sun Young Baek ),( Jin Ho Shin ),( Jae Ok Kim ),( Kyung Il Min ),( Seung Rel Ryu ),( Bok Soon Min ),( Do Keun Kim ),( Yong Seok Jeong ),( Sue Nie Park ) 한국미생물생명공학회 2005 Journal of microbiology and biotechnology Vol.15 No.3

A형간염바이러스의 정량검출을 위한 자동핵산추출과 연동된 One-step Real-time Reverse Transcription-polymerase Chain Reaction법 개발

김병국(Byoung-Guk Kim),정혜성(Hye-Sung Jeong),백선영(Sun-Young Baek),신진호(Jin-Ho Shin),김재옥(Jae-Ok Kim),민경일(Kyung-Il Min),류승렬(Seung-Rel Ryu),민복순(Bok-Soon Min),김도근(Do-Keun Kim),박미경(Mi-Kyung Park),안미진(Mi-Jin Ahn) 대한미생물학회 2003 Journal of Bacteriology and Virology Vol.33 No.3

PV MIC의 고승압ㆍ고효율을 위한 DC-DC 컨버터 토폴로지 및 성능분석

민병국(Byoung-guk Min),송성근(Sung-geun Song),오승열(Seung-Yeol Oh),김대경(Dae kyung Kim) 전력전자학회 2011 전력전자학술대회 논문집 Vol.2011 No.7

본 논문에서는 공진형 액티브 클램핑 플라이백 회로를 구성하여 전력스위칭의 전압 클램핑을 통하여 스위칭 손실을 저감하고자 하였다. 또한, 입력측 대용량 인덕터를 대신하여 공진형 액티브 클램핑 플라이백 회로에 두 개의 변압기를 사용하여 효율개선 및 고승압을 구현하고자 하였으며, 하드웨어 Prototype을 제작하여 타당성을 검증하였다.

중소형 선박용 항해기록장치 시스템 설계 및 구현

민병국(Byoung-Guk Min),모창환(Chang-Hwan Mo),김철원(Chul-Won Kim),박종훈(Jong-Hoon Park) 한국전자통신학회 2015 한국전자통신학회 논문지 Vol.10 No.3

본 논문에서는 중소형 선박에 적합한 저가의 VDR(Voyage Data Recorder)시스템을 설계하고, 해양 항법시스템의 효율성 향상을 위해 해양 항법시스템과 VDR이 시리얼 통신 혹은 이더넷 기반으로 데이터 통신을 하여 VDR에 연계된 항해통신 장비 및 센서 등의 데이터들을 항법시스템에 저장 및 출력할 수 있도록 구현하고자 한다. 또한, 육상에서 VDR의 기능과 동일한 역할을 수행하는 “차량용 블랙박스”(차량 위치, 영상, 음성저장)와 같이 VDR 고유 기능을 통한 정확한 해난 사고 원인 분석이 가능하게 중소형선박에도 확대 적용하고자 소형‧경량화로 설계하고자 한다. This study aims to design a chief VDR(Voyage Data Recorder) system which is appropriate to small and medium sized vessels and also implement the data about marine communication devices, sensors, etc. to be stored or printed at the navigator when those data are connected to VDR through data communication between marine navigation and VDR which are based on serial communication or internet in order to prove efficiency of the marine navigator. Also, the design of VDR is intended to be small and light in order to expand to apply it to small and medium vessels, which enables to analyze causes of marine accidents precisely through its characteristic functions which are the same as those at “vehicle mounted black-box” (location of the car, image and voice storage) by which the same roles are played on land.

디지털 헬스케어 가정용 유린분석기 기술 연구

민병국(Byoung-Guk Min),민순호(Sun-Ho Min) 한국정보기술학회 2021 한국정보기술학회논문지 Vol.19 No.2

The convergence digital medical devices and ICT are being highlighted as an important item that can prevent new diseases. In particular, due to the promotion of demand for digital healthcare following the popularization of smart devices and the overall impact of society in an aging era, medical expenses are being reduced and the culture of its own inspection system is changing. In this respect, diagnosis through a urine test is a test that allows you to conveniently check and manage your health status from time to time. In this paper, we improved our optical techniques to increase the accuracy of the urine analyzer and We established criteria for color analysis of red and green through numerical analysis. Also, we propose a urine analyzer based on IoMT(Internet of Medical Thing) that Block-Chain technology is incorporated. Urine analyzer is to reduce national medical costs as well as prevent the prevention of personal disease and promote personal health. Thus, we propose a digital healthcare home Block-Chain urine analyzer technology for secure analysis data on health and disease due to the proliferation of medical AI applications in the future.

수두백신의 역가시험법 표준화를 위한 연구

민경일,백선영,신진호,김재옥,류승렬,민복순,김병국,김도근,김훈,이석호,박순희 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

수두(chicltenpo즈) 및 대상포진(shingles)의 예방을 위해 사용되는 수두 생 바이러스 백근은 현재 국내 -외 제조사들이 정상사람 2배체 폐 태아 세포 주들을 이용하여 바이러스를 배양 · 증식시켜 생산하고 있으나, 품질관리를 쒸한 역가 시험에는 t·fRC튼 띤 LB딘EL 세포 주외에 제조사가 설정한 세포 주를 이용하고 있으며, 역파신험 방법인 fH Ui'rFO Plaque 3SSaIT에도 조사별로 다소 차이가 있는 시헌법을 사용하고 있다. 그러므로 일반적으로 세포 주간우 감수성 차이, 계대력(passa딩e level)간의 차이,varicelta-zoster virus(4'2V) diluent medium 등에 의한 차이에 의해 역가시힘 결과에 많은 차이를 나타낼 수 있다. 이에 본 연구에서는 역가 시험 결과꼭 편차를 촉소화 할 수 있는 시헌방법을 극힙하고자 Ok퍼 백신 주와 세로 주간 및 기타 요인에 따른 차이를 비교 분석하고 이를 표준확하여 일관겅있는 국가 및 제조사의 품질관리예 활용하고 나아가 수두 생 바이러스 백신 역가시헌 국가 표준품 거달에 활용하고자 하는데 목적이 운.다. 수두 생 바이러스 빅신의 역가 시험에 영향을 띠칠 수 꿀.는 모민으로 우선 바이러스 흡착에 사용되는 1#Z)·'diluent medium이 가장 큰 오인으로 확인되.고. 현개까기 최종적으로 if4 sucrose, 10% FBS, 0.1% sodiun) rrlonog)utamate in PBS(-), fIH 7.2가 가당 두수글 L'z).「 린luent medium 조성으로 확인되었단. 또한 '·:~l포 주별로 다소의 차이는 잇.으나 최대 바이러스 흡착시간은 90분으론:÷잰착인 되었다. 다른 negati)Fe effect를 보일 수 있는 요인으로 항생물질 및 항츤 제인 fuugiBone이 곡이러스의 역가에 영향을 미치는 것으로 확인되었다 따라서 이러한 항생물 띤 항균제를 standard solid agarose overla?·(5.tO) 시헌방법에는 사용하지 않는 것이 역가 시험의 편차를 줄일 수 있는 요인이라 판단된다. 최적의 조건하데서 Oka 수두 백신 주의 j 종의 세포 쭈에 대한 역가시험 수행 결과 각 세포 주의 감수성의 차이는 크지 않았으나, 반복시헌에 대한 표준편차는 다소 차이를 나타났다. Until now in Korea, the international and domestic vaccine maufacturers produce and sale live attenuated daricella vaccines, used to prevent chickenpox and shinglees, which are propagated in and done the potency test with various originated human diploid embryonic lung cells. Also manufacturers perform in vitro plaque assay with a little different methods to their quality control tests. Therefore, in general, there is slight or big difference among in vitro plaque assay results because of the difference in susceptibility of various originated human diploid embryonic lung cells and in assay methods. The purpose of this study is to confirm what factors occurring deviation to establish the standardized method which minimize deviation intracellularly and intercellularly. Eventually the standadized method will be used in consistent national and manufacturer's quality control and moreover intra and inter laboratory's validation assay of national standard for live varicella vaccine candidate. We confirmed VZV diluent medium was the biggest factor influencing in vitor plaque assay and the excellent composition of VZV diluent medium was final 5% sucrose, 10% FBS, 0.1% sodium monoglutamate in PBS(-), pH 7.2. We confirmed 90 minutes was maximum and optimum VZV adsorption time, but a little different among cells Also we recommended not use antibiotics and antimycotic, fungizone in standard solid agarose overlay assay in varicella potency test. Eventually 5 different originated human diploid embryonic lung cells showed little difference in susceptibility to Oka vaccine strain, but showed a lot of difference in reproducibility.

Survey on the Current Status of Laboratory Animal Quality Control Program in Korea

Byoung Chun Lee,Mee Kyung Jang,Kab Ryong Chae,Dae Youn Hwang,Byoung Guk Kim,Seung Wan Jee,Sun Bo Shim,Su Hae Lee,Ji Soon Sin,Chang Jun Bae,Jong-Min Woo,Jung Sik Cho,Kwang Soo Joo,Chuel Kyu Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.1

It is critical to harmonize the laboratory animal quality control program of each organization in order to improve the quality of laboratory animals. The aim of this study was to survey the current status of the laboratory animal quality control of 6 major animal suppliers and 14 Good Laboratory Practice (GLP) organizations. First of all, 6 suppliers provided more than 3 million animals, and 99% of them were specific pathogen-free (SPF) mice and rats. 88% of the GLP organizations were also using SPF animals, 81% of which were mice and rats. Two suppliers importing and offering animals were depending on the animal health report certified by providers, 2 suppliers were outsourcing to a domestic monitoring center, and 2 suppliers were monitoring in-house and simultaneously sending live animals to international centers for microbiological monitoring. All suppliers were monitoring the genetic integrity of inbred animals only once in a year. Fifty seven percentage of GLP organizations were doing the in-house microbiological monitoring, and only one of them was evaluating the genetic background of animals. Four suppliers monitoring animal quality were applying more than 5 samples of each animal facility unit quarterly. Fifty five percentage of GLP organizations monitored with each facility unit and 36% of them chose samples in the experimental group. Additionally, the animal number used in monitoring was 3-5, and monitoring frequency was 4 times in a year. Two suppliers sending animals to foreign companies for monitoring adopted almost all agents as testing items. Two suppliers were testing critical items including Ectromelia virus, Sendai virus, Mouse Hepatitis virus, Hantaan virus, Rat Coronavirus, Minute virus of mice, M. pulmonis, C. piliforme, and H. hepaticus. It were only 5 microoragnisms, MHV, Sendai virus, M. pulmonis, C. piliforme, and Ectoparasites which more than half of GLP organizations were monitoring commonly. Almost all organizations had their own SOPs for animal health monitoring and required a nationally harmonized guideline on the laboratory animal quality control. The result of this survey will be basic information on the national policy of the management of laboratory animal quality, and contribute to the improvement of laboratory animal quality in Korea.

Bacterial Composition of Respiratory Organs and Intestine in Mice and Rats

Byoung Chun Lee,Mee Kyung Jang,Jong-Kun Seo,Jin-Ho Kang,Kab Ryong Chae,Dae Youn Hwang,Byoung Guk Kim,Seung Wan Jee,Sun Bo Shim,Su Hae Lee,Ji Soon Sin,Chang Jun Bae,Jong-Min Woo,Chuel Kyu Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.1

The change of microbial flora in intestine and respiratory organs make some effects on animal health, which can cause some aberrations to experiment data. Therefore, it is an important factor to maintain the homogeneity of the bacterial composition of each organ. In this study, the bacterial distribution of respiratory organs and intestine were analyzed from 247 mice and 200 rats by analyzing biochemical properties and 16S rDNA sequencing. The total of 1,075 isolates were classified to 35 species of 17 genera. In case of rats, intestinal microbial flora were composed of 5 major bacteria including E. coli, P. mirabilis, M. morganii, C. freundii, and P. vulgaris, and 5 bacteria such as E. coli, P. mirabilis, S. aureus, S. cohnii, and M. morganii also mainly consisted of the microbial flora of respiratory organs. In case of mice, intestinal microbial flora were composed of 4 major bacteria including E. coli, P. mirabilis, A. lwoffi, and L. adecarboxylata, and 5 bacteria such as E. coli, B. sphaericus, S. auricularis, A. lwoffi, and L. adecarboxylata also mainly consisted of microbial flora of respiratory organs. This study suggested that B. sphaericus, G. morbillorum, S. paucimobilis, Staphylococcus spp. were specific in respiratory organs, and that M. morganii and Citrobacter spp. were almost specific in intestine. This study also can give some basic information to understand the relationship between animal care environment and microbial composition of animal organs.