활동기와 동면기 살모사의 위장관 점액세포내 점액질에 관한 조직화학적 연구

한현주,박래백,임영민,김효성,박경수,노영복 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1

살모사(Aekistrodon halys Pallas)의 위장관 점액세포내 점액질을 활동기와 동면기로 나누어 조직화학적으로 비교관찰하였다. 일반관찰을 위하여 H-E 염색을, 조직화학적 소견을 관찰하기 위하여 PAS reaction, Ab2.5 염색, AB1.0염색, AB2.5-PAS 염색, AB1.0-PAS 염색 AF1.7 염색 및 AF1.7-AB2.5 염색등을 시행하였다. 위(胃)는 표면점액세포와 점액경세포의 점액질을, 소장은 은롸롸 융모의 점액세포를 관찰한 결과를 다음과 같이 요약하였다. 1. 활동기와 동면기의 위점막에서는 현저한 차이는 없으나, 소장점막은 동면기에는 점막 주름수가 감소하고 높이가 낮아지며 점액세포가 위축되었다. 2. 위 표면점액세포는 활동기에 중성점액질과 약간의 Sialomucin을 포함한 산성점액질로 구성되었으나, 동면기에는 중성점액질과 Sialomucin이 약간 감소하였다. 3. 위 점액경세포는 활동기에 중성점액질만을 포함하고 있었으나, 동면기에는 Sialomucin을 포함한 산성점액질이 소량 검출되었다. 4. 소장 점액세포는 활동기에 산성점액질과 중성점액질로 구성되었으며 산성점액질에는 Sulfomucin과 Sialomucin이 포함되어 있었으나, 동면기에는 Sulfomucin과 Sialomucin이 다소 감소하였다. This study was performed in order to investigate the histochemical properties and compositions of the mucosubstances in the mucous of the gastrointestinal tract of Vipers, Agkistrodon halys Pallas, in active and hibernating phases. The specimens from stomach and small intestine were fixed in 10% neutral buffered formalin, embedded in paraffin wax, sectioned at a thickness of 4㎛, and stained with Hematoxyling-eosin for general histological observation and with PAS reaction, Alcian blue pH 2.5 or 1.0, Alcian blue pH 2.5-PAS or 1.0-PAS reaction, Aldehyde fuchsin pH 1.7, and Aldeliyde fuchsin pH 1.7-Alcian Blue pH 2.5 for histochemical finding. The mucous compositions in mucous cells of the gastrointestinal tract examined by their stain reaction were neutral mucin, acidic mucin, sulfomucin, sialomucin, The results obtained were as follows; The folds of the gastrointestinal tract in the hibernating phase, compared with the active phase, shrink and lower. Also, the shrinkage of epithelial cells were observed in the hibenating phase. The surface mucous cells of the stomach in the active phase composed mainly neutral mucin and a little amount of acidic mucin including sialomucin, but in the hibernating phase neutral mucin and sialomucin slightly decreased. The mucous neck cells of the stomach in the active phase constituted mainly neutral mucin, In the hebernating phase the mucous neck cells were composed of neutral mucin and a little amount of acidic mucin contained sialomucin. The mucous cells of the small intestine in the active phase consist of neutral mucin, acidic mucin including sulfomucin and sialomucin but in the hibernating phase sulfomucin and sialomucin slightly decreased.

항암제 AG60이 흰생쥐 위점막 상피세포의 DNA합성에 미치는 영향 : 자기방사법적연구

박경호,안의태,고정식,하상선,한영복,정영신,홍은경,유보림,김상건,이경영,강종구 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

스마트 미디어 카드를 이용한 교통사고 영상 저장 방법

이경복,박기현,한민홍 한국경영과학회 2001 한국경영과학회 학술대회논문집 Vol.- No.1(2)

This paper describes a method for saving traffic accident images in SMC(Smart Media Card), which is served as objective proof. SMC served being small-sized, strong, easy to attach and take off, which enables users image and voice-information service such as digital camera, MP3 Player, PDA and so on. Above all on account of it's high-speed in saving. The result of using SMC is saved pictures for 15 second before and after traffic accident image(320×240×8bit) into 8 frames/sec using a voice-recognition method or image recognition method. These pictures can be revived by PDA or mobile computer through up-loading serial/parallel communication or USB communication. This method could be applied to develop BLACKBOX.

두경부 유리피판 26례 분석

정복성,윤근철,심경보,고경석,한상훈 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.3

Twenty four patients(21 males and 3 females) aged 14 to 70 years underwent 26 microvascular free tissue transfers in head and neck reconstructions from April 1990 to July 1992 (27 months) . Nineteen cases were tumors in head and neck area, and four were acquired deformities such as burn alopecia, burn scar contracture of the neck, Romberg disease and frontal sinus infection, and one was hemifaclal microsomia. The free flaps were executed successfully in 15 upper aerodigestive tracts, in 2 scalps and 3 skull bases and in contouring and resurfacing six facial and cervical lesions. The selection of donor site was bases upon the location of defect and the volume of tissue required : the forearm free flap was used in 11 cases, the rectus abdominis in 5, the groin in 2, the jejunum in 2, the occipital scalp in one, the first toe web space in one, the latissimus dorsi in one, the serratus anterior in one, the parietotemporalis fascia in one and the omentum in one. The free tissue transfers were primarily indicated in all patients in our series and there was no flap failure. The complications were minimal and the morbidities low. A free flap in the head and neck area is not only the most expedient method of reconstruction but also either the most reliable or the only available one in some clinical situations.

보육시설 과학 특별활동 프로그램의 개발과 적용

조복희,곽혜경,한유미,양연숙,손경화 한국영유아보육학회 2003 한국영유아보육학 Vol.0 No.32

The purpose of this study was to develop early childhood science activities, which could be utilized as an extracurricular program in the day-care center. The program is based on the Constructivist theory(Piaget and Vygotsky) and the nation-wide survey data on the extracurricular programs and science activities in the day-care center. After the manuals for the program was monitored and corrected by the professionals, the application of each activities was empirically tested by the teachers in the day-care setting. The effects of the sample program(6 activities) were conducted using Tegano, Sawyers & Moran(1989)'s Scientific problem-solving scale and Lee(2001)'s Scientific attitude scale. Subject of thirty nine children were administered by the pre-test and post-test and experimental group participated the program for six weeks. The results showed that the program was effective for enhancement of scientific problem-solving ability and scientific attitude of young children in day-care setting.

한우의 간이물질 대사효소의 활성도

이관복,박승춘,한경오,윤호인 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.1 No.-

It is important to study the activity of drug biotransformation enzymes, because from a pharmacological and therapeutic point of view these enzymes are resposible for eliminating most drugs. Their level is ciritical when deciding the dose regimen. (From a toxicological perspective, the role of these enzymes varies, with some of them being directly responsible for activation of certain chemicals to reactive intermediates with deleterious consequence to the animal.) Experiments were designed to measure in vitro, the activity of drug metabolizing enzymes in liver of Korean native cattle. The microsomal monooxygenase activities were evaluated utilizing specific substrates. The activities of BPDM and TST 6β-hydroxylase were seemed to be high level relatively to other monooxygenzses such as ECOD, EROD and AH. Interestingly, TST 16α-hydroxylase which is a maker enzyme for CYP ⅠLA was not detected in Korean native cattle. These results sugest that Korean native cattle have high contents of CYP IIB1 and CYP IIIA.

蒼附導痰丸이 白鼠의 排卵에 미치는 影響

강복환,박종문,박경미,조한백,엄주오,유심근 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1

Changbudodamhwan(CBH) is used in female infertility caused by ovulation disorder. An attempt was made to evaluate the influences of CBH on the serum concentrations of FSH, LH, estradiol(E_(2)) and progesterone, the histological and optical changes of ovary, ovary weight of rats. The results of the study were as follows : 1. Blood FSH level increased experimental group as compared with control group, which showed no efficacy. 2. Blood LH level increased in experimental group as compared with control group, which showed no efficacy. 3. Blood E_(2) level increased in experimental group as compared with control group, which showed no efficacy. 4. Blood progesterone level increased in experimental group as compared with control group, which showed no efficacy. 5. In optically observations of ovary, weight of ovary increased in experimental group as compared with control group, which showed no efficacy. 6. In histological observations of ovary, ovulation increased in experimental group as compared control group, which showed efficacy. According to these results, CBH influence on the ovulation and ovary of rats.

가로조건에 따른 읍성마을의 좌향 및 배치특성에 관한 연구 : 낙안읍성과 성읍마을을 중심으로 Focused on the case of Nakan and Sungeup Villages

임창복,한경훈,김경완 대한건축학회 2004 대한건축학회논문집 Vol.20 No.7

The aim of this study is to verify the characteristic of the orientation and arrangement of the traditional settlement based on street condition. We select Nak-an, Sung-eup as example sites that were directed to the Cultural Properties protection Act in 1980's and well preserved their prototypes. We found some characteristics about the object. First, for street organization, Eup-sung style has direct order of the T type street, Second, for physical condition of the street, average width is 6-8m, road ratio is 25-30%. Third, is about orientation and arrangement based on street condition. If the building facing two roads which meet two directions, type arrangement and south orientation were common. They use side road to access the main building, direction of the gate is east, and have a strong tendency to locate in the side of site.

인천지역 대학생에 의한 대학급식소의 급식서비스에 대한 품질 평가

우경자,한복진,노정옥 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.3

This study was conducted to investigate the quality assessment of performance of the university foodservice. Self administered questionnaires were collected from 305 students living in Incheon. Statistical data analysis was completed using a SPSS v. 10.0 program. The results were summarized as follows: 61.4% of male students and 48.1% of female students responded to have lunch regularly. Only 23.4% of male students and 14.1% of female students used the university foodservice daily. Reasons for the irregularity of lunch were responded as "lecture", "eating when I want" and "appointment with friends", etc. The quantity, temperature, nutritional value, appearance, hygiene, taste and freshness of foods and price of menus were evaluated as appropriate but using seasonal foods, number of side-dishes, etc. as unsatisfactory. Service speed, cloth hygiene, neatness and kindness of employee were evaluated as appropriate. For the facilities of foodservice, counter location, menu board and lighting facilities were evaluated as appropriate but heating facilities, disponsition of tables and chairs of dining hall were as unsatisfactory. Sanitation of floors and walls of dining hall, restroom and utensils, etc. was evaluated as low.

활동기 척추동물의 십이지장 배상세포내 점액질에 관한 조직화학적 연구

박래백,한현주,임영빈,김효성,박경수,노영복 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1

본 실험은 다람쥐(Tamias sibiricus asiaticus Gmeli), 살모사(Agkistrodon halys Pallas), 황소개구리(Rana catesbeiana Shaw), 뱀장어(Anguilla japonica Temminck et Schlegel)등의 소장 배상세포내 점액질에 대한 성상을 밝히기 위하여 PAS reaction, Alcian blue pH2.5 및 Alcian blue pH0.4, 그리고 ABpH2.5-PAS reaction 복합염색등을 시행한 결과 다음과 같은 결론을 얻었다. 1. 사용한 각 동물에 있어서 소장 배상세포는 중성점액질, 약산성 및 강산성 황화점액질을 포함하고 있었다. 2. 중성점액질이 각 동물에 있어서 가장 안정된 분포를 보였으며 약산성 황화점액질도 비교적 고르게 분포되어 있었다. 3. 강산성 황화점액질은 다람쥐, 살모사 및 황소개구리에서는 소량 관찰되어 불안정한 분포를 보였으며 뱀장어에서는 관찰되지 않았다. 4. 각 실험동물의 십이지장 배상세포내에 포함된 점액질의 성상은 동물에 따라서 그 양과 염색성에 다소의 차이를 인정할 수 있다. In general, the goblet cells in the small intestines of experimental animals contained neutral mucosubstances, weakly and strongly acidic sulfated mucosubstances. Neutral mucosubstances show most stable distribution in the experimental animals. Mucosubstances in the small intestines of eels were composed of only neutral mucosubstances and weakly acidic sulfated mucosubstances. The contents and stain properties of mucostances produced in the intestinal goblet cells vary a little in portions within a species and in the same cell types of different species. This studies were performed in order to study composition of the mucosubstance in the goblet cells of small intestines of the various vertebrates. The specimens used in this study were obtained from the small intestines of 4 species of vertebrates, squirrels (Tamias sibiricus asiaticus Gmelin), vipers (Agkistrodon halys Palls), frogs (Rana catesbeiana Shaw), eels (Anguilla japonica Temminck et Scchlegel). All specimens were fixed in 10% neutral buffered formalin, embedded in paraffin wax, and sectioned at a thickness of 4㎛. They were stained with hematoxylin-eosin for general histological observation and with Periodic Acid-Schiff reaction, alcian blue pH2.5 and 0.4 and alcian blue pH2.5-Periodic Acid-Schiff reaction for detection of the mucosubstances. The mucous compositions in the goblet cells of the small intestines of various vertebrates examined by their staining reaction were neutral mucosubstances, weakly and strongly acidic sulfated mucosubstances.