배양계배 골격근 세포분화와 100-KDa 단백질의 양적 변화에 대한 Protein Kinase C(PKC)의 영향

신영아,안병태 가톨릭대학교 자연과학연구소 1995 자연과학논문집 Vol.16 No.-

PKC가 근세포 분화중에 미치는 영향을 살펴보기 위해서, 계배 근원 세포에 PKC 활성물질로 알려진 TPA와 DAG 유사물질인 OAG를 농도와 배양 시간을 달리하여 처리하였다. 그 결과, TPA와 OAG는 근세포 분화동안에 세포 융합을 촉진 하였다. 또한, PKC를 활성화하는 Ca^(2+)의 근세포 융합에 대한 역할을 알아보기 위해서, Ca^(2+)의 길항제로 알려진 TMB-8과 CaM의 길항제로 알려진 TFP를 처리했을 때 근세포 융합이 억제 되었다. Ca^(2+)-CaM에 의존적으로 인산화 반응을 하는 100-KDa 단백질에 대한 PKC의 효과를 알아보기 위해서, TPA와 TMB-8을 처리 하였다. TPA를 처리한 세포 추출물에서 100-KDa 단백질이 나타났으며, TMB-8을 처리한 세포 추출물에서는 100-KDa 단백질이 나타나지 않았다. 이러한 결과를 종합해 볼 때, PKC는 배양 근원세포의 막융합을 촉진하며, 부분적으로 Ca^(2+)-CaM에 의존적으로 인산화 반응을 하는 100-KDa 단백질에 영향을 미치는 것으로 사료된다. 그러나, Ca^(2+)/CaM-dependent protein kinase와 PKC가 100-KDa 단백질의 인산화 반응을 조절하는 신호 통합 기작 경로(signal transduction mechanism pathway)에 대한 연구는 더 필요 할 것으로 생각된다. To study the influence by protein kinase C on the differentiation of muscle cell, myoblasts of chick embryonic pectoral muscle were treated with PKC activator, TPA and diacylglycerol analogue, OAG and fusion indices were measured at various culture time. The result showed that TPA and OAG increased the fusion during the muscle cell differentiation. To identify the role of Ca^(2+) that activates PKC in the myoblast fusion, TMB-8, Ca^(2+) antagonist and TFP, calmodulin antagonist were treated. TMB-8 and TFP blocked myoblast fusion. To investigate the PKC effect on 100-KDa protein, TPA was treated. TPA was found to increase 100-KDa protein. Also, the treatment of TMB-8 decreased 100-KDa protein. These results suggested that PKC activate membrane fusion of myoblats and partially effect the 100-KDa protein. Further studies, however, are needed to reveal the relationship between Ca^(2+)/CaM-dependent phosphorylation of 100-KDa protein and PKC during the myoblast fusion.

발작성 고혈압을 주소로 한 급성 간헐성 포르피리아 1예

신정아,이지선,변영섭,고충원,이병권,김병옥,이건주 白中央醫療院 2004 仁濟醫學 Vol.25 No.1

Acute intermittent porphyria is a disorder that results from partial porphobilinogen deaminase deficiency and accumulation of overproducted porphyrin and its precusors. This makes variable manifestations such as abdominal pain, nausea, vomiting, contipation, peripheral neuropathy, seizure, psychotic symtoms, respiratory failure, tachycardia, and hypertension. When acute paroxysmal hypertension presents, it is difficult to differentiate from pheochromocytoma. We experienced a case of young female with acute intermittent porphyria presenting paroxysmal recurrent headache, palpitation, chest discomfort and paroxysmal hypertension. Initially blood pressure was 160/100 mmHg and checked high paroxysmally on follow up. Rhythm was sinus tachycardia. She did not complain any other specific symptoms. Although her clinical manifestations were suspicious for pheochromocytoma, laboratory findings were not compatible to it. In searching other causes of secondary hypertension mimickng ph대chromocytoma, elevated urine porphobilinogen level was detected and we could make diagnosis of acute intermittent porphyria. If AIP was not beared in mind as a cause of secondary hypertension, diagnosis would be delayed more.

PS와 PAMS의 오존에 의한 분해 반응

김아영,박남국,신재순,김영철 全南大學校 觸媒硏究所 2003 觸媒硏究 論文集 Vol.24 No.-

An investigation study showed the enhancement effect of ozone on the degradation of polystyrene (PS) and poly(α-methylstyrene) (PAMS). At various temperatures and concentrations of ozone, experiments were conducted, and the samples were taken and analysis by GPC (Gel permeation chromatography). We observed the degradation of PS and PAMS were enhanced at higher reaction temperatures, which cause chain scission vigorously, and at higher concentrations of ozone. For confirmation of ozone's nature as a strong oxidizer, the activation energy derived from the degradation by ozone additon was compared with those from thermal and catalytic degradation, and turned into much lower than the other cases.

Estradiol에 의한 흰쥐-자궁의 수축 기능과 Polyamine 대사의 변동에 관한 연구

이명아,고재홍,이영재,신경호,전보권,천연숙 고려대학교 의과대학 1995 고려대 의대 잡지 Vol.32 No.3

The polyamines have been postulated to be essential in most processes associated with both hypertrophy and hyperplasia. The effects of estrogen on uterine contractility vary with animal species, and most reports have shown that in vitro contractility of rat uterus is decreased after estrogen treatment, but estrogen increases polyamine contents in castrated rat uterus. This study was carried out to investigate whether the regulation of polyamine metabolism may be instrumental in the contractile response of castrated nonpregnant uterus of female SD rats to electrical field stimulation (40 V, 100 μsec : EFS). Estradiol (E2) increased uterine contents of putrescine (PT), spermidine (SPD), and spermine (SPM). These E2 effects were inhibited by tamoxifen or DFMO, but E2-incuced increase of uterine SPM content was enhanced by aminoguanidine. E2 decreased the amplitude of force developed in the rhythmic spontaneous contraction of rat uterus, but it exaggerated the increase of uterine contraction force in response to EFS. The E2-dependent exaggeration was inhibited by tamoxifen, DFMO, or indomethacin, but was significantly enhanced by aminoguanidine. The synergism between E2 and aminoguanidine on uterine contractility was little affected by indomethacin. These results suggest that E2-dependent increase of uterine contractility may be ascribed partly to increase of uterus polyamines.

기관지 방선균증의 임상 1례

이은영,장태원,정만홍,옥철호,이규원,손창배,이지숙,신은경,서정아,백종현,김영옥 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Actinomycosis is a chronic suppurative infection, whitch infiltrates mucosa-associated tissues. Thoracic, pelvic and cervicofacial infections of actinomyces are not uncommon, but endobronchial actinomycosis is rarely reported. We report a case of a 29 year old man who presented with a recurrent pneumonia and chronic cough. Fiberoptic bronchoscopy revealed occlusion of the right. lower medio-basal segment bronchus with exophytic endobronchial mass. The diagnosis was confirmed histologically by demonstration of sulfur granules in the bronchoscopic biopsy of the mass. Intravenous administration of amoxacillin and clindamycin followed by oral amoxacillin and clindamycin therapy resulted in complete recovery.

암치료에 있어서 체질과 항암효과에 관한 한의학적 연구

성현제,신현규,박갑주,강봉주,은영아,김은해,정세영 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

As one of the chemical and experimental study on cancer treatment in Korea oriental medical hospital. Furthermore, we carried out the cytotoxicity test of extracts of herbal medicine. The results are as follows : About Clinical Aspects of the Oriental Medical Cancer Treatment - (1) Clinical experience is that 420 Oriental medical hospital were surveyed. Number of cancer patient treated by one oriental medical doctor was under 10(about 60%) or from 10 to 20(about 30%). (2) Diagnostic Procedure is that more than 80% of cancer patients were diagnosed by medical doctor of western hospital. (3) Therapeutic Method is that combination of oriental and western medical treatment was used in oriental medical hospital. (4) Relationship between cancer and constitutions is that to the relationship between cancer and constitutions, about 75% responded and most were considered to be Um. About Experimental Study - to evaluate the direct cytotoxicity of hot water and methanol extracts of 34 oriental medical presciptions on stomach cancer cell, SRB assay was carried out. (1) The IC50 value of water extract of Samsoum was 19㎍/㎖ and that of methanol extract was 15㎍/㎖, so that we confirmed Samsoum has a strong direct cytotoxicity. (2) The IC50 values of water extract of Coptidis Rhizoma, methanol extract of Caesalpiniae Lignum and Draba Semen were 10㎍/㎖, 19㎍/㎖ and 12.5㎍/㎖ respectively, so that we confirmed Coptidis Rhizoma, Caesalpiniae Lignum and Draba Semen have a strong direct cytototoxicity. (3) The IC50 value of combinatory prescription of Rhei Rhizoma, Forsythie Fructus and Caesalpiniae Lignum was about 40㎍/㎖.

STZ 유발 당뇨쥐에서 시간에 따른 생리적 항산화계의 변화

이순재,양정아,김성옥,최정화,신주영,채영미,차복경 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.5 No.-

The purpose of this study was to investigate change of physiological antioxidative system according to the time in streptozotocin-induced diabetic rats. Sprague-Dawley male rats weighing 150±10gm were randomly assigned to one normal and five STZ-induced diabetic groups. Diabetic groups were classified to experimental period. Diabetes was experimentally induced by intravenous injection of 55 mg/kg of body weight of STZ in citrate buffer(PH 4.3) after 6 weeks feeding of three experimental diets. Animals were sacrificed at 0, 3, 6, 12, 18th days of diabetic states. 1. Activity of superoxide dismutase(SOD) in liver was increased in that of diabetic mellitus(DM) groups at 3th day but that of DM groups was not significant from 6th day according to period. Glutathion peroxidase(GPX) was significantly decreased in DM groups from 6th day 2. Reduced glutathione(GSH) contents in liver significantly decreased in diabetic groups from 3rd day compared with those of DM groups according to the period. Oxidized glutathione(GSSG) was higher from 6th day. GSH/GSSG ratio was significantly lower than that of normal group from 3rd day to all experimental period 3. Contents of vitamin E in liver of DM groups were significantly decreased compared with that of normal group from 6th day. 4. Lipid peroxide(LPO) contents in liver of DM groups were significantly increased compared with that of normal group from 3rd day. The present results indicate that STZ-induced diabetic rats were reduced by antioxidative defense system and taken by peroxidate damage in tissue compared with normal group from 3rd day or 6rd day after injection STZ. It lended to acceleration all diabetic groups but no significance according to the experimental time.

비만세포주에서 Histamine 유리에 관한 연구

김형룡,국윤아,신민,이홍수,원진숙,김형민,이영미,정헌택,이혜숙,안녕형 원광대학교 생명공학연구소 1995 생명공학연구소보 Vol.3 No.1

Mini-implant를 이용한 III급 부정교합 환자의 협진 치료 : 문제중심의 교정치료

임동혁,김영신,조민아,김기성,양성은 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.4

갑상선 유두암이 병발된 가족성 대장 용종증 1예

백종현,소창배,이은영,이지숙,신은경,박은호,서정아,양재홍,송준영,박선자,박요한,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome, typically characterized by multiple colorectal adenomas and increased incidence of colorectal carcinomas if it is not treated. Moreover, a variety of extracolonic manifestation are seen. The prevalence of thyroid tumors developing in patients with FAP is about 1∼2%. Generally, papillary thyroid cancer indicates low mortality but ,however, as people get older, the cancer becomes more aggressive. So, the operation is required. Recently we experienced a case of FAP, presenting with papillary thyroid carcinoma, and reported with a brief review of literatures.