Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

[Book Review:] Yi Jin-sang’s “the Theory of Sim jeuk ri (Mind is the Principle),” in Complete Works of Hanju.

( Choi Young-jin ) 성균관대학교 유교문화연구소 2014 儒敎文化硏究(中文版) Vol.0 No.21

충청지역 판소리 문화 유적 현황 연구

최혜진(Choi, Hye Jin) 한국구비문학회 2018 口碑文學硏究 Vol.0 No.48

본 논의는 충청지역의 판소리와 관련된 인물들과 지역을 살피고, 발굴해야 할 문화유적과 기초자료의 중요성을 강조한 글이다. 특히 충남지역의 판소리적 위상을 살피면서 현장 답사를 시행하였고, 판소리 지형도를 살펴, 시급히 조사 연구해야 할 명창과 유적에 대해 논하였다. 먼저 조선창극사에 나타난 충청지역의 명창 23명과 후대의 주요 명창, 명고 등 35명의 생애과 정보, 의의를 정리하고 충남지역이 판소리 발생기와 일제강점기에 매우 중요한 역할을 하였음을 밝혔다. 다음으로는 현지 답사를 중심으로 홍성, 서산, 서천, 공주, 논산 지역의 명창 관련 정보와 문화유적 등을 조사하여 보고하였다. 홍성에서는 판소리의 시조 최선달의 가계와 묘역이 밝혀졌으며, 김창룡의 주거지 등이 남아있음을 알 수 있었다. 한성준 관련 여러 유적도 제대로 보전되어야 할 필요성이 있다. 또한 동편제의 다른 맥을 만든 정춘풍에 대한 지역조사가 시급하다. 서산에서는 심정순 일가와 연관되어 낙원식당 등이 중요한 유적지가 될 수 있으며, 이를 활용할 필요가 있다고 보았다. 고수관 등의 유적지를 조사하였고 방만춘 일가 조사의 필요성을 제기하였다. 서천에서는 이동백과 김창룡 관련 유적지가 발굴되고 보호되어야 한다. 공주에서는 박동진 전수관의 현황을 둘러보았고, 황호통이 중요한 명창으로 활약했음을 밝혔다. 이와 함께 황해천, 김석창, 박상도, 이동백과 김창룡, 고수관 등이 활약했고 거주했던 지역으로 공주의 판소리 문화를 함께 연구할 필요성을 논했다. 논산은 중고제의 시조라 불리는 김성옥이 태어난 곳이고, 그의 생가터가 추정 발굴되고 있다. 이에 대한 관심이 필요할 것이다. 초기 판소리는 충남지역을 중심으로 위로는 경기권, 아래로는 전라권으로 뻗어나가 판소리 문화를 확산 개발시킨 중요한 역사적 사명을 감당하였다. 현재적 의미에서 중고제는 충청지역을 중심으로 발굴, 보전되어야 할 필요가 절실하며 이를 위한 기초연구가 시급히 시행되어야 한다. 흩어져 있는 중고제 관련 명창과 유적지의 정보를 최대한 모아서 충청도 판소리의 위상을 세우는 작업을 하는 일이 필요하다. 중고제 소리를 제대로 전승할 수 있는 실력있는 명창을 보호하고 육성할 필요도 있다. This article emphasizes the importance of cultural heritages that should be developed upon reviewing persons related to Joonggoje Pansori in the Choongchung area and its basic data. It reviews, in particular, the position of Pansori in South Choongchung by dividing the territory of Pansori, and discussing the master singers and heritages that should be prioritized. First, it summarizes the information about 23 master singers in Choongchung, the history of Chosun traditional opera, and 35 masters in the late period, including their lifetime achievements and meanings, and reveals that they played a crucial role in the development period of Pansori in South Choongchung, and in the Japanese colonial period. Next, it is reported that the information and cultural heritages relate to the master singers in Hongsung, Seosan, Seochun, Gongju, and Nonsan, focusing on field visits. In Hongsung, the family history and the boundaries of Sun Dal Choi’s grave, the founder of Pansori, were found, and Chang Ryong Kim’s residence was revealed. It is necessary to conserve a variety of heritages related to Sung Jun Han. In addition, it is urgent tolocally investigate Choonpoong Jung who developed the different roots of Dongpyunje. In Seosan, the Nakwon restaurant, and so on, relate to the family of Jung Soon Shim, and could be an important historical place, which should be utilized. A historical place, such as Gosoogwan, was investigated, suggesting the needs to investigate the family of Man Choon Bang. In Seochun, the historical places related to Dong Baik Lee and Chang Ryong Kim should be developed and conserved. In Gongju, the statuses of Dong Jin Park and Soo Gwan Go were reviewed, and it was revealed that Ho Tong Hwang had been performed as an important master singer. Together with this, further studies are required on Pasori culture in Gongju, where Hae Chon Hwang, Suk Chang Kim, Sang Do Park, Dong Baik Lee, Chang Ryong Kim, and Soo Gwan Go were actively performed and lived. Nonsan is where Sung Ok Kim, a founder of Joonggoje, was born, and his place of residence is under development. Hence, attention in this is required. Joonggoje Pansori took part in the important historical mission to expand and develop Pansori culture up to the Gyunggi area, to the North, and Cholla area, to the South, in the center of South Choongchung. It is urgently required that Joonggoje Pansori is developed and conserved in the center of the Choongchung area, which requires basic research on this. It is necessary to establish the position of Choongchung Pansori by collecting the information of master singers and historical places related to Joonggoje, which are scattered around. Also, it is necessary to protect and develop the master singers who are well placed to succeed Joonggoje Pansori.

Localization of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal Antibody

Kang-seuk Choi,Jin-ju Nah,Young-joon Ko,Shien-young Kang,Yi-seol Joo 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal AntibodyKang-seuk Choi*, Jin-ju Nah, Young-joon Ko, Shien-young Kang1 and Yi-seok JooNational Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea1Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, KoreaReceived April 2, 2003 / Accept July 10, 2003J. Vet. Sci. (2003), 4(2), 167-173JOURNAL OFVeterinaryScience*Corresponding author: Kang-seuk Choi National Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea Tel: +82-31-467-1860, Fax: +82-31-449-5882 E-mail: choiks@nvrqs.go.kr

Ehlers - Danlos 증후군

주용진,최성문,최영자,성호석 ( Yoing Jin Joo,Sung Moon Choi,Young Ja Choi,Ho Suk Sung ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

A Case of Ehlers-Danlos syndrome Yoing Jin Joo, M.D., Sung Moon Choi, M.D., Young Ja Choi*, M.D., Ho Suk Sung, M.D. Department of Dermatology, Clooego of Medicine, Inje University, Pusan, Korea We presented a case of Ehlers-Danlos syndrome in a 25-year old female, who had hyperextensibility of skin, skin fragility, cigarette paper-like atrophic scar, joint hyperextensibility and skeletal abnormality of scoliosis since birth. There was no family history. Microscopic and electron microscopic examinations of the skin biopsy specimens from normal appearing skin on the forearm and the site of subcutaneous nodule was compatible with finding of Ehlers-Danlos syndrome. Although the manifestations of this case including marked extensibility of skin, joint, skin fraility and atrophic scar might be in accord with type Ⅰ(gravis) Ehlers-Danlos syndrome, skeletal abnormality of scoliosis did not correspond to this type, but to type Ⅳ(hydroxylysine deficient) Ehlers-Danlos syndrome. We consider this case as a type Ⅳ Ehlers-Danlos syndrome.

유치와 영구치에서 치과용 접착제의 전단결합강도

최진영,최남기,박영준,최충호,양규호 大韓小兒齒科學會 2007 大韓小兒齒科學會誌 Vol.34 No.4

The objective of this study was to compare the shear bond strengths of five adhesive systems to the enamel and dentin of primary and permanent teeth. Fifty noncarious primary and fifty permanent teeth were collected and stored in an 0.1% thymol solution at room temperature after extraction. The tested adhesives were: Adper Scotchbond Multi-purpose Plus Adhesive (SM) Adper Single bond 2 (SB), Clearfil SE Bond (SE), Adper Prompt L-Pop (PL), G-Bond (GB). For the shear bonding test, the labial and lingual surfaces of primary and permanent teeth were used. To obtain a flat surface, the labial and lingual surfaces of the teeth were sanded on SiO₂with number 600 grit and then divided into 20 groups of 10 surfaces each. All samples were theromocycled in water 5℃ and 55℃ for 1000 cycles. The results were as follows: 1. For primary enamel, shear bond strengths of SM and SB were significantly higher than that of SE and also SM, SB, and PL were higher than GB(p<0.05). 2. For primary dentin, there were no significant differences among the shear bond strengths of any other bonding systems except differnece between SE and GB. 3. For permanent enamel, SB showed significantly higher mean shear bond strength than those of any other bonding systems(p<0.05). 4. For permanent dentin, SM showed significantly higher mean shear bond strength than that of PL and GB(p<0.05). 5. Between the primary enamel and dentin, there were significant differences in SM, SB, and GB, whereas there was statistically significant difference in PL between the permanent enamel and dentin(p<0.05). 6. Between the primary and permanent teeth on enamel, there were no significant differences among all bonding systems, whereas there were statistically significant differences in SM and SB between the primary and permanent teeth on dentin(p<0.05). 본 연구에서는 최근에 개발된 치과용 접착제를 대상으로 유치와 영구치의 법랑질과 상아질에 대한 결합력을 비교 평가하여 입상에서 상아질 접착제를 적절하게 선택하는데 도움을 주고자 시행하였다. 실험재료로 Adper Scotchbond Multi-purpose Plus Adhesive (SM; 3M ESPE,USA),Adper Single bond 2 (SB; 3M ESPE,USA),Clearfil SE Bond (SE; Kuraray Medical Inc.,Japan),Adper Prompt L-Pop (PL; 3M ESPE,USA),G-Bond (GB:GC Cooperation Toyko,Japan)를 이용하여 전단결합강도를 평가하여 다음과 같은 결과를 얻었다. 1. 유치 법랑질에서 전단결합강도는 SM이 28.47±7.93 MPa로 가장 높았고 SB (28.18±6.84),PL (24.02± 6.14),SE (21.70±6.56),GB (17.70±6.68) 순이었다. SM과 SB는 SE보다 통계적으로 유의하게 높은 결합 강도를 보였으며,SM,SB,PL은 GB보다 높았다(p<0.05). 2. 유치 상아질에서 전단결합강도는 SE가 28.72±14.44MPa로 가장 높았고,PL (20.10±6.13),SM 07.72± 10.65),SB (15.48±2.66),GB (10.10±5.45) 순이었으며 SE와 GB간 에는 유의한 차이가 있었으나,다른 군 들 간에는 유의한 차이가 없었다. 3. 영구치 법랑질에서의 전단결합강도는 SB가 28.36±5.68 MPa로 가장 높았고 SM (22.77±4.63), PL(22.05 士6.57),SE (21.74±4.62),GB (15.60±6.75)순이었다. SB는 다른 접착제보다 통계적으로 유의하게 높은 결합력을 나타냈으며,GB는 가장 낮은 결합력을 보였다(p(0.05). 4. 영구치 상아질에서의 전단결합강도는 SM이 28.22±5.56 MPa로 가장 높았고,SB (21.68±7.44),SE (20.13 ±9.88),GB (14.30±6.81),PL (14.18±5.88)순이었으며,SM은 PL,GB와 유의한 차이가 있었다(p<0.05). 5. 유치의 법랑질과 상아질 간에는 SM,SB,GB에서 유의한 차이가 있었고,영구치 법랑질과 상아질 간에는 PL에서 유의한 차이가 있었다(p<0.05). 6. 유치 법랑질과 영구치 법랑질 간에는 모든 접착제에서 유의한 차이가 없었으나,유치 상아질과 영구치 상아질 간에는 SM과 SB에서 유의한 차이가 있었다(p<0.05).

Streptococcus salivarius의 요소분해효소 유전자 변이에 관한 연구

최혜진,이진용,최호영 大韓齒科保存學會 1999 Restorative Dentistry & Endodontics Vol.24 No.4

Polyphosphate가 Porphyromonas endodontalis의 성장에 미치는 영향에 관한 연구

최성백,최호영,민병순,박상진,이진용,최기운 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.1

Polyphosphate has been used to prevent decomposition of foods and has been shown to have inhibitory effect on the growth of gram positive bacteria. The purpose of this study was to evaluate the effect of polyphosphate on the growth of Porphyromonas endodontalis, a gram negative endodontopathic bacterium. Porphyromonas endodontalis ATCC 35406 was grown in the presence of polyphosphates with different chain lengths. Inhibitory effect of each polyphosphate which was added at the beginnig or during the culture, was determined by measuring the optical density of the bacterial cell at 540nm and by viable cell count. The results from this study were as follows : 1. Polyphosphates were shown the growth inhibition of the Porphyromonas endodontalis. 2. The minimal inhibitory concentration(MIC) of polyphosphate was observed to be 0.04%. 3. Polyphosphates with chain lengths of 25 and 75 demonstrated the greatest inhibitory effect on the growth of Porphyromonas endodontalis. 4. Polyphosphates are bactericidal to Porphyromonas endodontalis, demonstrating the growth inhibition of the bacterium. The overall results suggest that use of polyphosphate may affect the growth of Porphyromonas endodontalis. Further studies will be needed to confirm the effect of Polyphosphate.

조혈모세포이식 환자에서 발생한 Cytomegalovirus 질환의 특징 : 일개 대학변원에서 최근 10년간의 경험

최수미,이동건,박선희,김시현,김유진,민창기,김희제,이석,최정현,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2009 감염과 화학요법 Vol.41 No.1

Background : Studies on cytomegalovirus (CMV) diseases in Korean hematopoietic stem cell transplant (HSCT) recipients are lacking and do not reflect the recent trends of advances and changes. Therefore, we tried to analyze the clinical features of CMV diseases in HSCT recipients over the past 10 years at a tertiary university hospital in Korea. Methods : Retrospective review of medical records was done for all adult HSCT patients who received transplant at the Catholic HSCT Center from January 1998 to January 2008. Results : Forty-four cases (2.2%) of CMV diseases were identified. CMV pneumonia was diagnosed in 17 patients, retinitis in 16 patients, enterocolitis in 7 patients, esophagitis 1 patient, gastritis in 1 patient, duodenitis in 1 patient, and hepatitis in 1 patient. The median onset of symptom was 90 days after transplantation. Late CMV diseases accounted for 47.7%. CMV related death varied from 0 to 58.8% according to the involved organ. CMV retinitis was diagnosed relatively later in the course of transplantation mostly in patients who had chronic graft versus host disease (GVHD). On the contrary, CMV enterocolitis mainly occurred in patients who suffered from acute GVHD. The overall concurrent CMV reactivation was documented to be 63.6%: the concurrent CMV reactivation was observed only in 37.5% of patients with retinitis. Conclusions : We observed some differences in the pattern of CMV disease manifestation according to the involved organ and reconfirmed the fact that CMV pneumonia is the most common and fatal disease in HSCT recipients. Additionally, CMV retinitis was not uncommon in HSCT recipients. Since specific marker does not exist in predicting retinitis, regular ocular examination should be done thoroughly, especially in patients with chronic GVHD.

Reliability Considerations of Hybrid Power Systems for Critical Facilities

Choi, Se Jin · Ham, Young Hwan · Kim, Myung Chin 충북대학교 컴퓨터정보통신연구소 2008 컴퓨터정보통신연구 Vol.27 No.2

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