Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

Bi-factor MIRT True-Score Equating for Testlet-Based Tests

이규민(Gue min Lee),Won Chan Lee,Michael J Kolen,박인용(In Yong Park),Dong In Kim,Ji Seung Yang 한국교육평가학회 2015 교육평가연구 Vol.28 No.2

Lee and Lee (2014) proposed bi-factor multidimensional item response theory model (BFM) "observed-score" equating procedures. The main purposes of this study were to develop BFM "true-score" equating procedures, and to investigate applicability of the proposed procedures with actual data. Eight equating methods (including both true- and observed-score) based on dichotomous IRT (2-parameter logistic model: 2PL), polytomous IRT (graded response model: GRM), testlet response model (TRM), and BFM were compared with target equipercentile equating. Data for this study were from the Reading Comprehension test for a large-scale state assessment program, which consisted of several passages and corresponding groups of items. True- and observed-score equating methods based upon 2PL and BFM produced similar equating results. The GRM true- and observed-score equating methods provided equating results somewhat different from others, and more similar to the target equipercentile equating. Lee와 Lee(2014) 연구는 혼합형 검사에서의 bi-factor 다차원 문항반응모형을 적용한 IRT "관찰점수" 동등화 절차를 제안하였다. 이 연구는 Lee와 Lee(2014) 연구의 추수연구로 단위검사를 대상으로 bi-factor 다차원 문항반응모형을 적용한 IRT "진점수" 동등화 절차를 제안하고, 그 적용 가능성을 실제 자료를 이용하여 탐색하고자 하였다. 이를 위해 단위검사로 구성된 대규모 독해 검사 자료를 이용하여, 2모수 로지스틱 모형(2-parameter logistic model: 2PL), 등급반응모형(graded response model: GRM), 단위검사모형(testlet response model: TRM), bi-factor 모형(bi-factor model: BFM)을 적용하였으며, 관찰점수 및 진점수 동등화를 시행하여 총 8개의 동등화 방법을 적용하고, 산출된 결과를 동백분위 동등화 결과와 비교하였다. 연구 결과, 2PL과 BFM을 적용한 관찰점수 및 진점수 동등화는 유사한 결과를 산출하였으며, GRM을 적용한 관찰점수 및 진점수 동등화 방법은 다른 방법들과 다소 차이를 보이는 결과를 산출하였고, 비교 기준이 된 동백분위 동등화 결과와 보다 유사한 결과를 보였다.

도덕적 행동에 영향을 미치는 자아관련 변인 간 구조적 관계: 예비교사와 현직교사의 비교를 중심으로

이지혜(Lee Ji Hye) 학습자중심교과교육학회 2015 학습자중심교과교육연구 Vol.15 No.8

본 연구는 예비교사와 현직교사의 도덕적 행동에 관심을 갖고, 이에 영향을 미치는 자아관련 변인들(자기성찰, 자기통제력, 도덕적 정체성) 간의 관계를 Blasi(1980)의 자 아모형(self-model)에 논리적 근거를 두고 이지혜(2015)가 제시한 구조방정식모형이 예비교사와 현직교사 간에 어떠한 차이가 있는지를 밝히는데 목적을 두었다. 이를 위 하여 본 연구는 충북에 소재한 2개 대학교 예비교사를 대상으로 303개의 자료를, 그 리고 충북에 소재한 중학교 4개교와 고등학교 2개교에 재직 중인 현직교사를 대상으 로 333개의 자료를 Amos 20.0으로 최종 분석하였다. 분석결과는 다음과 같다. 첫째, 이지혜(2015)의 연구에서 제시한 모형을 예비교사와 현직교사 전체를 대상으 로 적용해본 결과, 구조방정식모형의 적합도는 χ2=625.173(df=128, ρ=.000), RMSEA=.078, GFI=.908, TLI=.942, CFI=.948, NFI=.933으로 모든 기준을 충족하였다. 따라서 예비교사를 대상으로 검증한 이지혜(2015)의 구조방정식모형은 예비교사뿐만 아니라 현직교사를 포함하여 적용하는 것도 가능하다는 것을 알 수 있다. 둘째, 이상 의 구조방정식모형을 예비교사와 현직교사 모형으로 분리하여 분석한 결과, 두 모형 모두 적합도 지수 기준에 충족하는 것으로 나타났다. 그러나 예비교사와 현직교사 모 형에는 통계적으로 유의한 차이를 보였다. 구체적으로 모든 경로에서 유의한 영향력 이 있는 것으로 나타난 예비교사와는 달리 현직교사의 경우 도덕적 정체성이 도덕적 행동에는 유의한 영향력이 없는 것으로 나타났다. 따라서 도덕적 행동을 높이기 위해 서는 예비교사와 현직교사에게 차별적인 조력이 필요할 것이다. The aim of this study was to investigate the moral behavior of pre-service teachers and in-service teachers. Using the self-model of Blasi(1980) as a theoretical basis, we analyzed the relationships between self-related variables(self-reflection, self-control, moral identity) that influence moral behavior, and we aimed to reveal the differences between pre-service teachers and in-service teachers using the structural equation model suggesting by Ji-Hye Lee(2015). To this end, we performed a final analysis using Amos 20.0 on data from 303 pre-service teachers in 2 universities situated in Chungbuk, and data from 333 in-service teachers at 4 middle schools and 2 high schools in the same area. The results were as follows. First, when we applied the model of Ji-Hye Lee(2015) to pre-service teachers and in-service teachers, the fit of the structural equation model fulfilled all criteria, with χ 2=625.173 (df=128, ρ=.000), RMSEA=.078, GFI=.908, TLI=.942, CFI=.948, and NFI=.933. We showed that the structural equation model of Ji-Hye Lee (2015), which was originally validated on pre-service teachers, could be applied to in-service teachers as well as pre-service teachers. Second, when we analyzed the above model separately for pre-service teachers and in-service teachers, both models were found to fulfill criteria for fit indices. However, there was a statistically significant difference between the pre-service teacher model and the in-service teacher model. In other words, unlike the pre-service teacher model, in which there is a significant effect in all pathways, for the in-service teacher model, there was no statistical effect of moral identity on moral behavior. Accordingly, in order to increase moral behavior, different assistance will be required for pre-service and in-service teachers.

지역주민의 삶의 질에 관한 주관적 의식 분석

이지은(Lee, Ji-Eun),이재완(Lee, Jae-Wan) 한국지방정부학회 2014 지방정부연구 Vol.18 No.1

이지은(Lee, Ji-Eun), 이재완(Lee, Jae-Wan) 본 연구의 목적은'삶의 질'에 대한 지역주민의 인식을 유형화하고 분석하는 데 있다. 지역주민의 삶의 질에 관한 주관적 인식을 분석하기 위해 Q방법론을 활용하였다. 지역주민을 대상으로 한 인터뷰 및 선행연구 검토를 통해 Q진술문을 작성하였고, P샘플은 서울시에 거주하고 있는 지역주민 40명을 대상으로 하였다. 분석 결과, 삶의 질에 대한 주민인식은 ① 기본적 요건 지향, ② 물질적 요건 지향, ③ 비물질적 요건 지향으로 유형화되는 것으로 나타났다. 본 연구는 삶의 질에 관한 인식이 지역주민마다 다를 수 있으므로, 지방자치 수준에서 숙의민주주의를 실현할 수 있는 공론의 장을 마련할 필요가 있다는 점을 시사한다. 지방정부가 지역주민들의 삶의 질을 향상시키는 정책을 수립할 때 다양한 주민들의 인식과 의견이 반영될 수 있도록 하여야 할 것이다. The objective of this study is to classify and analyze citizens' perceptions on 'quality of life'. In order to examine quality of life as perceived by citizens, Q-methodology was used. A set of Q-statements was constructed with reference to field interviews of citizens and literature review of the precedent study, and it was rated by 40 citizens residing in Seoul. The results of this study revealed that citizens' perceptions of quality of life could be classified in terms of three perspectives: 1. Basic conditions orientation, 2. Material conditions orientation, and 3. Non-material conditions orientation. Based on the analysis results, this study suggests that as citizens' perceptions of quality of life can be diverse, there is a need to develop a public sphere where deliberative democracy would be realized and advanced at local government level. By creating and developing public spheres, local governments would be able to take citizens' perceptions and opinions into account better when establishing policies for improving citizens' quality of life.

Collective “We” and the Communal Consciousness of Diaspora Identity in Chang-rae Lee`s On Such a Full Sea

이지은 ( Ji Eun Lee ) 서울대학교 미국학연구소 2014 미국학 Vol.37 No.2

This paper examines how Change-rae Lee`s On Such a Full Sea imagines a communal consciousness of diaspora identity through the use of a collective narrative voice that creates a narrative sense of split identity in readers` minds. The novel`s use of heroine figure, Fan, as a locus of communal consciousness very much corresponds to the conventional framework of the Bildungsroman narrative, which builds a communal consciousness incorporated into a progressive history through a depiction of the individual hero`s or heroine`s reconciliation with social norms. The narrative consciousness grounded in this individual hero/heroine invites readers to experience the sense of community congruent with the linear development of history. Lee`s novel, however, complicates the narrative consciousness through a collective narrative voice “we,” by shifting the referent from “we” inside the narrative frame to “we” outside the narrative frame and by dislocating the main figure Fan from society. The sense of disjointedness manifested by the narrative sense of split identity and the heroine`s displacement in society conceptualizes diaspora identity. Diaspora identity configured in this way propounds a more liberating version of communal consciousness as it allows deviations from norms and exploration of new possibilities not bound by nation or ethnicity. The communal consciousness of diaspora identity depicted in Lee`s novel challenges the homogeneous sense of community merged with history and gestures toward heterogeneous deviations from history.

소음인(少陰人) 태음병(太陰病) 심하비증(心下?證)의 병증약리(病證藥理) 고찰

서영광,이지원,이준희,최원철,이의주,고병희,Seo, Young-Kwang,Lee, Ji-Won,Lee, Jun-Hee,Choi, Won-Cheol,Lee, Eui-Ju,Kho, Byung-Hee 사상체질의학회 2010 사상체질의학회지 Vol.22 No.3

1. Objective: Gastric Stuffiness (心下?證) in the Soeumin Taeeum symptomatology (少陰人 太陰病) in Sasang Constitutional Medicine shows an interesting evolutionary process. Originally found in the texts of Sanghanlun (傷寒論), the perspectives regarding the symptoms of Gastric Stuffiness were expanded by many medical scholars and Lee Jema. To comprehend the Jema's concept of Taeeum symptomatology and utilize his prescription clinically, it deserves to study on the Gastric Stuffiness in Donguisuseowon (東醫壽世保元). 2. Methods: Many texts including Sanghanlun (傷寒論) and Dongeuibogam (東醫寶鑑) were reviewed in order to track the evolutionary process concerning the symptoms of Gastric Stuffiness. Also, Lee Jema's works including the different versions of Donguisuseowon (東醫壽世保元) were reviewed to examine the development of Lee's concepts regarding the Gastric Stuffiness in the Soeumin Taeeum symptomatology. 3. Results and Conclusions: 1) Most of the previous concepts on Gastric Stuffiness were generally similar to those found in Sanghanlun, especially in that they addressed Yang deficiency of the middle triple energizeer as the central pathogenic process. 2) Lee Jema's views on the epigastric discomfort in the Soeumin Taeeum symptomatology evoloved over time and culminated in the finalized pathology and therapeutics appearing in his last work, the Shinchuk version of Donguisuseowon (東醫壽世保元). 3) 4 kinds of prescription which invented by Lee Jema to treat the Gastric Stuffiness in the Soeumin Taeeum symptomatology can be matched to the prescription of Sanghanlun respectively. However through evolutionary process of Jema's prescriptions, the indication of them expanded: not only to treat Gastric Stuffiness but to diverse symptoms of Soeumin.

부산·울산·경남지역 직업병 감시체계

김정일,김병권,김정원,채창호,이철호,강동묵,김지홍,김진하,김영욱,이영하,이지호,김정호,윤형렬,유철인,정백근,장태원,김운규,윤동영,강진욱,김종은,안진홍,이동준,장준호,이광영,송혜란,최영희,이용환,조병만,최홍렬,고상백,김은아,이유진,홍영습,정갑열,김정만,김준연 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.1

Objective: Occupational medicine specialists in the Busan, Ulsan and Kyung-Nam areas established an area-based occupational disease surveillance system and used this system to collected case information for the purpose of preventing occupational diseases Methods: l l hospital participated in this system. The authors selected five main diseases, which were hand-arm vibration syndrome (HAVS), work-related musculoskeletal disorder (WRMSD), occupational asthma, occupational skin disease and occupational and occupational lung cancer and established their case definitions. All cases were reported on the web, and real time analysis was conducted. Results: Between April 2001 and April 2003, 192 cases of HAVS, 118 cases of WRMSD, 33 cases of occupational asthma, 17 cases of occupational skin disease and 17 cases of occupational lung cancer (for a total of 377 cases of these five main diseases) were reported. most of the HAVS cases came from shipbuilding, and 172 of them (92.7%) were associated with grinding. Of the four main types of WRMSD, carpal tunnel syndrome (CTS) was the most prevalent with 46 cases and shipbuilding was also the main industry involved (83.9%). The ergonomic risk factors involved mainly associated with the hand. In 19 (57.6%) and 4 (12.1%) cases of occupational asthma, the agents involved. The causative agents of occupational lung cancer included Cr, welding fume, PAH, Ni, etc. 10(58.8%) of the cases were diagnosed as squamous cell carcinoma and 5(29.4%) as adenoma. Conclusion: This result showed that an area-based occupational disease surveillance system might provide an effective method of evaluating the prevalence of such diseases, however the Busan, Ulsan and Kyung-Nam provinces are too large to be treated as individual areas. Therefore, the authors suggest that each province should establish its own surveillance system.

Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

The Effect of Personality as a predictor of SLA

Ji-hu Lee 영미어문학회 2004 영미어문학연구 Vol.20 No.1

Lee, Ji-hu. 2004. The Effect of Personality as a predictor of SLA. Tire British and American Language and Literature. 20-1. 99-114. This paper aims to examine the effect of personality as a predictor of second language acquisition. Even in the same language environment, some learners acquire a second language better than others. Moreover, certain individual characteristics affect how much individual learners use each processor although everyone has the same innate language processing mechanisms. It may be natural that the individual who wants to achieve a particular goal devotes considerable effort to achieve this goal. The individual who is an extrovert might have more opportunity of using the target language and practicing it. There is a principled distinction between formal, conscious learning and informal, unconscious acquisition in second language researches. Therefore, this study focuses on which part of English learning, such as listening, speaking, reading, and writing, that 10 Korean students in Indiana University of Pennsylvania (IUP), PA, tend to focus on because of their personality.

가축에서 분리된 Vancomycin 내성 장구균의 내성유전자 구조 분석을 통한 분자생물학적 역학 연구

허지영,이선민,이석호,이위교,용동은,이경원,신완식,이동건 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2