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Kim, Jae-Ho,Jeong, Seung-Chan,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1
안지오텐신(ACE) 저해제는 항고혈 효과를 갖고 있으므로 오랫동안 고혈압의 예방이나 치료에 이용되어 왔다. 본 연구는 재조합 대장균으로부터 새로운 ACE 저해제를 생산하고 정제하며 나아가 이들이 구조-기능 관P를 규명하기 위해 수행되었다. Saccharomyces cerevisiase의 ACE 저해 펩타이드 유전자를 함유하고 있는 재조합 pGEX-4T-3을 대장균 BL21(DE3)로 형질전환 시켰다. 재조합 pGEX-4T-3을 갖고 있는 대장균 BL21(DE3)로부터 생산된 Glutathione-s 전이효소 (GST) 융합 단백질을 얻어서 그중 ACE저해 펩타이드를 Sephadex G-25 컬럼 크로마토그래피로 정제하였다. 정제된 ACE 저해 펩타이드는 타이로신-아스파틱엑시드-그리신-글리신-발린-패닐알라린-아르기닌-발린-타이로신-트레오닌의 서열을 가진 새로운 decapeptide이었고 ACE에 대하여 경쟁적으로 저해하였다. The angiotensin I-converting enzyme (ACE) inhibitor has anti-hypertensive effects and has long been used as prevention or remedy of hypertension. This study were carried out to produce and purify a new ACE inhibitor from recombinant E. coli and further elucidate its structure-function relationship. Recombinant pGEX-4T-3 containing ACE inhibitory peptide gene of Saccharomyces cerevisiae was transformed into E. coli BL21(DE3). Glutathione-S transferase (GST) fusion protein from E. coli BL21(DE3) harboring the recombinant pGEX-4T-3 was obtained and the ACE inhibitory peptide was purified with Sephadex G-25 column chromatography. The purified ACE inhibitory peptide was a novel decapeptide with sequence Tyr-Asp-Gly-Gly-Val-Phe -Arg-Val-Tyr-Thr which shows very low similarity to the other ACE inhibitory peptide sequence. The purified ACE inhibitor competitively inhibited ACE.
류재호,노규태,이영석,이영호,권혁찬,김재석,김효진,이영민,박혜원,박근희 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1
연구배경: 동일한 환자를 대상으로 조혈모세포이식 전 일반 병실에서의 관해유도 항암제치료 및 강화요법으로 인한 호중구감소 기간의 감염양상과 고용량 항암제치료 후 무균실에서의 조혈모세포이식 시 호중구감소 기간의 감염양상을 중심정맥관 관련 패혈증을 중심으로 비교 연구함으로써 효율적인 감염관리지침을 마련하고자 하였다. 대상 및 방법: 1999년 1월부터 2001년 7월까지 2년 7개월간 동아대학교병원 일반병실에서 악성혈액질환으로 항암제 치료를 받다가 조혈모세포이식을 시행받았던 22명을 대상으로 중심정맥관 관련 패혈증의 형태를 비교분석하였다. 결과: 대상 환자들의 일반병실과 무균실에서의 입원기간은 각각 32±13일, 33±19일, 호중구 감소기간은 15±12일, 17±18일, 호중구 감소기간의 발열 횟수는 14회, 14회로서 입원병실의 종류에 따른 차이는 없었다. 환자들의 중심정맥관 사용일수는 일반병실에서 29일(7~545일), 무균실에서 100.5일(25~606일)이었으며, 총 사용일수는 각각 1,515일, 3,250일로서 무균실에서의 중심정맥관 사용일수가 의미 있게 길었다(P=0.001). 중심정맥관 사용 일수에 대한 감염률은 각각 5.28/1,000일, 3.08/1,000일로서 일반병실에서 그 빈도가 높았으나 통계적 유의성은 보이지 않았다(P=0.141). 결론: 중심정맥관 삽입 후 일반병실에서 항암제 치료를 받던 환자가 조혈모세포이식을 위하여 무균실에 입원하는 경우, 중심정맥관을 교체하는 것이 감염관리를 위하여 바람직할 것으로 생각된다. Background: We compared the incidence of central venous catheter (CVC) related septicemia during neutropenic period in the same patient, which developed following chemotherapy for remission induction and consolidation, or hematopoietic stem cell transplantation (HSCT). Methods: We retrospectively reviewed the medical records and laboratory data of 22 patients with hematologic malignancies who received anticancer chemotherapy followed by HSCT at Dong-A University Hospital between January, 1999 and July, 2001. We investigated the duration of hospitalization, duration of neutropenic period, duration of catheterization, microbiologically documented organisms and incidence of CVC related septicemia. Results: The data in general ward (before HSCT) and laminar air flow room (after HSCT) were as follows: duration of hospitalization was 32±13 days and 33±19 days, duration of neutropenic period was 15±12 days and 17±18 days, median duration of catheterization was 29 days (7~545 days) and 100.5 days (25~606 days) (P=0.001), and incidence of CVC related septicemia was 5.28/1000 days and 3.08/1000 days, respectively. In the CVC related septicemia, the most common etiologic organism was coagulase negative staphylococcus. Conclusion: We suggest that the exchange of CVC before admission to laminar air flow room could decrease the incidence of CVC related septicemia in HSCT recipients.
이재형,김찬룡,박상철 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.4
The objective of the research which it sees ' analyzed the leisure activity participation actual condition and a shedding of blood of the young people and with the drafter fee provision for a leisure activity activation further there is a place where it presents the leisure activity activation plan which is healthy ', in Pusan area. the south which is in the process of attending in the high school. 772 person schoolgirls (249 person junior high school students, is and and a question investigation in the object 239 person total high students whom it bites and 284 person industrial world high students) it did. It analyzes the leisure activity participation actual condition which it follows in sex and the school star and a shedding of blood and a leisure activity activation plan it uses SAS program with the method for, x² and with one-way ANOVA and time after death verification to execute Duncan test, the result which it analyzes, with afterwords escape it did a same conclusion. first, the leisure activity shedding of blood which the young people hope travels, relative visit, field activity and motion picture, play viewing, sports activity, the computer, shopping, sports viewing, music activity and the fine arts activity, appeared with group activity order. Second, when it presents the leisure activity activation plan which the young people is healthy it is a propulsion and support magnification back which magnification, leisure activity program development and supply, young people group activity and sports activity activation and young people policy of system leisure education execution, the gain and loss against the young people and leisure hour magnification, leisure facility expension, leisure leader arrangement and positive education is consistent.
이재원,신찬배 嶺南大學校 工業技術硏究所 2001 工業技術硏究所論文集 Vol.29 No.2
An accuracy of a heating coil is used to compare the performance of a proportional plus integral(PI) controller to the following scheme for improved learning controller: a reinforecement learning agent trained to minimize the sum of the squared error over time. Although the Pl controller works very well for this task. The reinforcement learning agent, when combined with a Pl controller, learned to augment the Pl control output for the subset of states for which control can be improved. keyword : Reinforcement learning control, PI control, HVAC, Heating coil
스포츠 스폰서십 요인과 스포츠 소비자 행동에 관한 연구
이근모,김찬룡,이재형 釜山大學校 附設 體育科學硏究所 2001 體育科學硏究所 論文集 Vol.17 No.-
The purpose of this study was to analyze that sport Consumer behaviors related sports sponsorship through meta- analysis. The result of this study was concluded as follows: 1. Male, teenager affirmed factors related sports sponsorship, act an good purchase intent more than female. 2. Higher sports participation degree groups affirmed factors related sports sponsorship, act as good purchase intent. 3. Company advertisement participated sports sponsorship affirmed, company image's reliability, and act an good purchase intent more than company advertisement not participated sports sponsorship. 4. Sports sponsorship affirmed reliability, development company image, development reliability, attitude that related company image act an good purchase intent.
Channel stamping 법으로 전이된 Cu pattern의 형상
이영훈,곽재찬,강봉석,김희철,최병호,Lange, F.F. 國立金烏工科大學校 産業技術開發硏究院 2002 産業技術開發硏究 Vol.18 No.-
A matallo-organic solution was stamped from the channels of a polymer stamp onto a glass. The precursor solution consisted of copper 2-ethylhexanonate, 2-ethoxyethanol as a solvent and ethylene glycol to improve surface energies. The solution was deposited to the polymer stamp by spin coater. After drying the solution in channels of the stamp, the dried ink was transferred to a substrate by debonding from channels. The process parameters such as solution compositions and pressing methods were discussed experimentally to form a copper pattern whose morphology was excellent.