Comet 분석을 통한 방사선처리 고추세포의 핵 DNA 손상평가

안정희,백명화,김재성,정정학,권순태,An, Jung-Hee,Back, Myung-Hwa,Kim, Jae-Sung,Jeong, Jeong-Hag,Kwon, Soon-Tae 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3

We employed single cell gel electrophoresis method (comet assay) to analyze the degree of nucleus-DNA damage in the leaves of red pepper (Capsicum annuum L.) seedlings exposed to $^{60}$ CO v-radiation stress. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length (H) in individual comet image isolated from pepper leaf cell. The T/H ratio of control-cells and treated-cells at 50 or 100 Gy were 1.28 and 3.54 or 3.39, respectively, suggesting that nuclei of pepper cells were severely damaged in the integrity of DNA strand by the treatment of enhanced v-radiation. The percentage of head-DNA in control-cells was 76.8%, whereas those of 50 and 100 Gy treated-cells were 55.9% and 59.9%, respectively. Pretreatment of low dose (4 to 20 Gy) radiation to seeds decreased DNA-damage in the leaves of seedlings treated with high dose radiation at 50 or 100 Gy. In this experiment, we developed a sensitive, reliable and rapid method for evaluating genotoxic effect in the nuclei of plant cells by employing comet assay. 방사선에 노출된 고추유묘의 잎으로부터 세포핵을 분리하여 단세포전기영동방법인 comet 분석을 통하여 핵 DNA의 손상정도를 조사하였다. Comet 분석에서 꼬리부분의 길이 (T)와 머리부분의 길이 (H)를 측정하여 T/H 비율을 조사하였다. 무처리세포는 T/H 비율이 1.28이었으나 50 및 100 Gy의 방사선을 처리한 세포는 각각 3.54 및 3.39로 방사선처리에 의해 상당량의 핵 DNA가 손상을 입은 것으로 나타났다. Comet의 head-DNA량은 무처리가 76.8%였으나 50 및 100 Gy를 처리한 세포는 각각 55.9% 및 59.5%를 보였다. 고선량의 방사선을 처리하기 전에 미리 20 Gy 이하의 저선량 방사선을 종자에 전처리하였을 경우 종자의 발아 및 생장에 대한 영향은 없었지만, 후속 고선량에 대한 핵 DNA의 손상은 경감되는 경향을 보였다.

방사선과 자외선에 대한 고추 식물체 및 배양세포의 생장반응과 Capsidiol 생합성 유전자의 발현 차이

안정희,김재성,정정학,오세명,권순태,An, Jung-Hee,Kim, Jae-Sung,Jeong, Jeong-Hag,Oh, Sei-Myoung,Kwon, Soon-Tae 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2

Differential responses of red pepper plant and cultured cells to enhanced ${\gamma}$-ray($^{60}$ Co) and ultraviolet(UV) stress were investigated. In seed treatment, 1 Gy of ${\gamma}$-ray increased seedling dry weight up to 19.1%, but 50 Gy treatment markedly ingibited seed germination and subsequent growth of seedling. UV treatment to seed did not change the germination ability of seeds and the growth of seedlings regardless of duration of UV treatment until 24 hrs. In case of UV treatment to seedlings, plant injury was seriously progressed even after the seedlings were returned to no UV condition, and eventually all the leaves showed chlorosis by the stress. However, progress of plant injury by ${\gamma}$-ray stress slower than that caused by UV stress, and even at the high dose of ${\gamma}$-ray 50 Gy, did not caused the cholrosis of stressed plant leaf. Amount of electrolytes leakage from plant leaf by UV treatment for 24hrs was increased up to 28.8 folds in comparison with untreated control, whereas that of 50 Gy of ${\gamma}$-ray was increased only 1.2 folds. UV stress induced the production of capsidiol, antimicrobial phytoalexin, by activation of gene expression involved in capsidiol biosynthesis, such as sesquiterpene cyclase and cyclase and cytochrome P450 hydroxylase in the leaf and cultured cell, but ${\gamma}$-ray stress induced neither the production of capsidiol nor expression of the genes.

친환경 식품과 가공 식품에 대한 주부들의 인식 및 소비 형태

안정희 ( Jeong Hee An ),강근옥 ( Kun Og Kang ) 한국식품영양학회 2006 韓國食品營養學會誌 Vol.19 No.1

공기공급량이 음식폐기물의 퇴비화 반응에 미치는 영향

정준오(Joon Oh Jung),안정희(Jeong Hee An) 大韓環境工學會 2001 대한환경공학회지 Vol.23 No.6

건조방법을 달리한 늙은 호박의 품질 특성 및 항산화 효과

허유정,김경지,송다혜,윤진아,정강현,안정희,Hur, Yoo Jeong,Kim, Gyeong-Ji,Song, Da hye,Yoon, Jin-A,Chung, Kang-Hyun,An, Jeung Hee 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.6

The objective of this study was to determine the effects of drying methods on the quality characteristics and antioxidant activities of pumpkin (Cucurbita moschata Duch.). Pumpkins were dried naturally ($25^{\circ}C$), hot-air drying ($60^{\circ}C$), and throgh freeze drying ($-40^{\circ}C$) methods. The moisture activities were highest in the freeze dried group. The soluble solid showed no significant differences among all groups. The pH was highest in the freeze dried group. The L values were increasing in freeze dried group, whereas the b values were increased in hot-air dried group. The free sugar was highest in freeze dried group. The DPPH radical scavenging activity of hot-air dried group was higher than those of the other groups. The ABTS radical scavenging activities were highest with hot-air dried group and freeze dried group. It was established that hot-air dried group is the most effective drying method for the production of high quality dried pumpkin.

아마란스 꽃 추출물의 항산화에 관한 연구

조현주,김정원,윤진아,김경임,정강현,송병춘,안정희,Jo, Hyeon-Ju,Kim, Jeong Won,Yoon, Jin-A,Kim, Kyoung Im,Chung, Kang-Hyun,Song, Byeong Chun,An, Jeung Hee 한국식품영양학회 2014 韓國食品營養學會誌 Vol.27 No.2

본 연구는 아마란스의 붉은 색과 보라색 꽃 열수 추출물과 메탄올 추출물의 폴리페놀과 플라보노이드 함량 측정과 DPPH와 ABTS 라디칼 소거 활성, SOD 유사 활성을 측정하였으며, 세포내에서 생성된 superoxide 라디칼 제거 활성과 산화질소 생성 억제 활성을 분석하여 새로운 식물 유래 라디칼 소거 활성 물질을 개발하기 위하여 시행하였다. 총 폴리페놀의 함량은 아마란스 추출물 중 보라색 꽃 메탄올 추출물이 606.95 mg GAE/100 g으로 가장 높았으며, 플라보노이드 함량도 254.69 mg CE/100 g으로 가장 높았다. 또한 DPPH 라디칼 소거 활성에서도 보라색 꽃 메탄올 추출물의 $RC_{50}$ 값이 $155.06{\mu}g/m{\ell}$로 나타났다. ABTS 라디칼 소거능 측정에서는 $250{\mu}g/m{\ell}$의 농도에서 보라색 꽃 메탄올 추출물의 활성이 53.16%로 가장 좋았으며, 보라색 꽃 열수 추출물(41.55%), 붉은 꽃 열수 추출물(30.52%), 붉은 꽃 메탄올 추출물(30.34%)의 순으로 활성을 나타내었다. 이와 반대로 SOD 유사 활성은 보라색 꽃 열수 추출물에서 메탄올 추출물의 활성보다 3배나 높은 결과를 보여주었다. 세포내 superoxide 라디칼 제거 활성은 $200{\mu}g/m{\ell}$의 농도에서 보라색 꽃 메탄올 추출물(72.34%)이 붉은 색 꽃 열수 추출물(40.40%)보다 1.79배 높은 활성을 보였다. 세포내 NO 생성 억제 활성을 조사한 결과에서는 보라색 꽃 메탄올 추출물이 $250{\mu}g/m{\ell}$의 농도에서 46.90%의 가장 높은 저해 활성을 보여주었다. 본 연구의 결과, 플라보노이드 함량이 높은 보라색 꽃 메탄올 추출물에서 라디컬 소거능이 높았으며 강력한 항산화제 활성을 보여주었다. 이러한 결과로 보아 아마란스 꽃의 새로운 항산화 소재로서 개발 가능성을 보여주었다. This study investigates the free radical-scavenging activities of Amaranth (Amaranthus spp. L.) red and purple flower extracts. The methanol and hot water extracts of flower are being evaluated for its total polyphenol and flavonoid contents, scavenging activities by the DPPH and ABTS analysis, SOD-like activity, and inhibition activities of superoxide radical on the HL-60 cells and nitric oxide of the RAW 264.7 cells. The PFM (purple flower extracted with MeOH) showed the highest total phenolic and flavonoid content, 606.95 mg GAE/100 g and 254.69 mg CE/100 g, respectively. Amongst the scavenging activities of the DPPH radicals, PFM($RC_{50}=155.06{\mu}g/m{\ell}$) is the highest of all the samples. The ABTS radical-scavenging activity is also highest for PFM (53.16%) at the $250{\mu}g/m{\ell}$ concentration. But, the SOD-like activity of the PFW (purple flower extracted with hot water) increases more than 3 folds of the PFM. In the leukemia HL-60 cell, the PFM shows strongly inhibited superoxide radical generations at a concentration of $200{\mu}g/m{\ell}$ at 72.34%, which increases with 1.79 folds more than the RFW (red flower extracted with hot water). The inhibition activity of nitric oxide in Raw 264.7 cells is the highest for PMF (46.90%) at a $250{\mu}g/m{\ell}$ concentration. In conclusion, PMF show the highest flavonoid contents and the most powerful free radical-scavenging activity. Our results suggest that the increase of antioxidant activities depend on flavonoid contents. Thus, Amaranth flower can be useful for natural antioxidant compounds.

레몬그라스 분말을 첨가한 머핀의 품질특성 및 항산화 효과

이정화,김경지,노규아,정강현,윤진아,안정희,Lee, Jeong Wha,Kim, Gyeong-Ji,Rho, Kyu-A,Chung, Kang-Hyun,Yoon, Jin-A,An, Jeung Hee 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.5

레몬그라스 분말을 첨가한 머핀의 품질특성 및 항산화를 측정하기 위하여 레몬그라스 분말 0%, 1%, 3%, 6%, 9%를 첨가하여 머핀을 제조하였다. 머핀의 높이, 부피 및 비체적은 분말의 첨가량이 증가할수록 감소하였다. 레몬그라스 분말 첨가군의 수분 함량과 점도는 대조군과의 유의적 차이가 나타내지 않았으며, 색도 측정 결과, 명도와 황색도는 레몬그라스의 농도가 증가할수록 유의적으로 감소하였으나, 적색도는 증가하였다. Texture를 측정한 결과, 레몬그라스 분말의 씹힘성은 첨가량이 증가할수록 감소하였다. 관능검사는 색, 향, 맛, 조직감, 기호도를 측정했으며, 그 결과 3% 첨가군에서 가장 높은 관능검사 결과를 보여주었다. 레몬그라스 머핀의 DPPH 라디칼 소거능 측정 결과, 레몬그라스의 농도가 증가함에 따라 유의적으로 항산화 활성이 증가하였으므로, 레몬그라스의 첨가가 머핀의 기능성에 좋은 영향을 나타낼 것으로 사료된다. This study investigated the quality characteristics and antioxidant effect of muffins that were prepared with the following lemongrass-powder concentrations: 0%, 1%, 3%, 6%, and 9%. The lemongrass-powder concentrations were added to the flour during the basic-formulation process, whereby the heights and volumes decreased significantly when the amount of lemongrass powder was increased. As a result of measuring the colors, the lightness and yellowness of the muffins decreased, whereas the redness increased with the increases of the lemongrass-powder concentrations. When the textures of the lemongrass muffins were measured, the chewiness and adhesiveness decreased with the increases of the lemongrass-powder concentrations. When the sensual qualities of the muffins were measured, the colors, flavors, tastes, textures, and overall quality of all of the samples show that the 3% lemongrass-powder concentration revealed the highest sensual-quality indexes. Further, the DPPH and ABTS radical-scavenging activities increased according to the concentration of lemongrass powder, whereby the former increased significantly when the amount of lemongrass powder was increased.

항암화학요법의 구토유발 수준별 예방적 항구토제 프로토콜의 이행정도

최자윤(Choi Ja Yun),오현정(Oh Hyeon Jeong),강지영(Kang Ji Young),김민경(Kim Min Kyoung),김지은(Kim Ji Eun),김진하(Kim Jin Ha),김희숙(Kim Hee Suk),박소라(Park So Ra),변정선(Byun Jeong Seon),안정희(An Jeong Hee),조민경(Cho Min Kyoung) 대한종양간호학회 2011 Asian Oncology Nursing Vol.11 No.1

Purpose: The purpose of this study was to identify the compliance with the protocol, which was developed considering the emetogenic potential for prophylaxis of chemotherapy. Methods: Data was collected from 144 patients who received chemotherapy from June 15 to August 31, 2010 in C University Hospital in Jeollanamdo, Korea. The level of chemotherapy-induced nausea and vomiting (CINV) and the compliance with the protocol for prophylaxis of CINV were measured. Results: There was statistically significant difference of CINV in morning sickness and anticipatory nausea of general and clinical characteristics. Also, the compliance with the protocol developed according to emetogenic potential of chemotherapy was statistically significant. There was no difference in CINV in regard to the compliance with the protocol. Conclusion: There was a good compliance with the protocol for prophylaxis according to emetogenic potential. But it should be recommended to use antiemetics for prophylaxis aggressively to relieve CINV for the patients who already experienced morning sickness and anticipatory nausea. In addition, the oncology nurses should respond sensitively to the complaints of nausea and vomiting no matter what the emetogenic potentials of chemotherapy regimen are.

당 침지액 농도에 따른 건조 둥근 마의 품질 특성 및 항산화 효과

조혜민 ( Hye Min Jo ),김희영 ( Hee Young Kim ),허유정 ( Yoo Jeong Heo ),송다혜 ( Da Hye Song ),김경지 ( Gyeong-ji Kim ),김인수 ( In-su Kim ),윤진아 ( Jin-a Yoon ),정강현 ( Kang-hyun Chung ),안정희 ( Jeong Hee An ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.6

This study was investigated the quality characteristics and antioxidant of dried Dioscorea bulbifera with various pre-soaking concentrations of oligosaccharide. Dioscorea bulbifera are prepared by additions of 0, 4, 6, 8 and 10% oligosaccharide solution, and dried at 50℃. The effects of pre-soaking percent of Dioscorea bulbifera slices were evaluated by the moisture, soluble solid, pH, titratable acidity, color, browning degree, texture, antioxidant activities and sensory test. According to the percent of pre-soaking oligosaccharide solution was increased, the moisture was increased but soluble solids and titratable acidity were decreased. With respect to the result of colors, Dioscorea bulbifera slices that underwent the 10% pre-soaked process (85.86%) were lighter than control (73.88%). However, the redness and yellowness scores were the lowest than control. The springiness and cohesiveness of texture showed no significant differences among all groups. Gumminess and chewiness of texture results were increased according to per-soaking concentration increase. Also the polyphenol, flavonoid and DPPH (α, α-diphenyl-β-picrylhydrazyl) and ABTS [2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging activities were significantly increased with increasing immersion concentration. The Dioscorea bulbifera slices supplemented with 6% pre-soaking oligosaccharide solution treatment showed the highest total sensory score. The results of our study indicated that when pre-soaking oligosaccharide solution is used to immerse the Dioscorea bulbifera slice, it has browning inhibition and antioxidant effect.

차광 및 혐기처리에 따른 청차의 성분과 향미 특성 변화

권미진(Mi Jin Kwon),손용휘(Yong-Hwi Son),이재순(Jae Sun Lee),정광희(Gwang Hui Jeong),조경환(Kyung Hwan Cho),황정규(Jung Gyu Hwang),김민정(Min Jung Kim),신지훈(Jihun Shin),신의철(Eui-Cheol Shin),안정희(Jeung Hee An),김종철(Jong Cheol 한국차학회 2021 한국차학회지 Vol.27 No.4

찻잎은 수확 전⋅후 외부스트레스에 의해 뇌기능개선, 혈압조절 기능이 있는 가바함량이 증가한다. 본 연구는 수확 전 차광처리와 수확 후 혐기처리를 통해 찻잎의 가바함량을 증진시켜 각각을 청차로 제다하여 그 성분과 품질을 비교해 보았다. 생엽의 혐기처리 조건은 탄산가스 주입 후 25℃에서 24시간 반응 시키는 조건에서 가바함량이 315.1 mg/100 g으로 가장 높았다. 가바와 테아닌 함량은 비차광 대비 차광 조건에서 증가하였다. 그중가바함량은 차광⋅혐기조건에서 182.7 mg/100 g으로 가장 높았다. 반면 테아닌은 혐기조건에서 감소하는 경향을 보였다. 카페인함량은 차광조건이 비차광조건, 혐기 보다 무혐기에서 높았으며, 카테킨함량은 비차광조건이차광조건보다 높았으며, 혐기처리시 감소하는 경향을 보였다. 테아플라빈함량은 차광 유무에 따른 차이는 없었으나, 혐기처리시 증가하는 경향을 보였다. 맛분석 결과 차광⋅혐기처리에서 무혐기처리 대비 신맛, 우마미는감소하는 반면, 짠맛, 단맛, 쓴맛, 떫은맛, 매운맛 등이 증가하였다. 전자코를 통한 향기 성분은 비차광⋅무혐기, 차광⋅혐기에서 propane, propenal, butane이 주요 성분으로 확인 되었으며, 비차광⋅혐기, 차광⋅무혐기에서는propane, propyzamide이 주요 성분으로 확인 되었다. The content of γ-aminobutyric acid (GABA), which improves brain function and regulates blood pressure, increases in tea leaves due to external stress before and after harvesting. This study compared the ingredients of oolong tea by increasing the GABA content in tea leaves through shading before harvest and anaerobic treatment after harvest. In the anaerobic treatment of raw leaves, the highest GABA content was 315.1 mg/100 g under the condition of reacting at 25℃ for 24 hours after carbon dioxide gas injection. The content of GABA and theanine had increased in oolong tea under the shading condition compared to the non-shading condition. The GABA content was the highest in oolong tea at 182.7 mg/100 g under shading and anaerobic conditions. On the other hand, theanine tended to decrease under anaerobic conditions. Caffeine content was higher in the shaded condition than in the non-shading condition, and it was higher in the aerobic condition than in the anaerobic condition. The catechin content was higher in the non-shading condition than in the shading condition and tended to decrease during anaerobic treatment. There was no difference in the theaflavin content with or without shading, but it tended to increase during anaerobic treatment. In the case of oolong tea, sourness and umami decreased compared to aerobic treatment in shading and anaerobic treatment, while salty, sweet, bitter, astringent, and spicy taste increased.