서구사고와 전통사고의 마찰

안정희 한국사법행정학회 1983 사법행정 Vol.24 No.10

Comet 분석을 통한 방사선처리 고추세포의 핵 DNA 손상 평가

안정희,백명화,김재성,정정학,권순태 한국식물생명공학회 2004 JOURNAL OF PLANT BIOTECHNOLOGY Vol.31 No.3

We employed single cell gel electrophoresis method (comet assay) to analyze the degree of nucleus-DNA damage in the leaves of red pepper (Capsicum annuum L.) seedlings exposed to 60Co -radiation stress. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length (H) in individual comet image isolated from pepper leaf cell. The T/H ratio of control-cells and treated-cells at 50 or 100 Gy were 1.28 and 3.54 or 3.39, respectively, suggesting that nuclei of pepper cells were severely damaged in the integrity of DNA strand by the treatment of enhanced -radiation. The percentage of head-DNA in control-cells was 76.8%, whereas those of 50 and 100 Gy treated-cells were 55.9% and 59.9%, respectively. Pretreatment of low dose (4 to 20 Gy) radiation to seeds decreased DNA-damage in the leaves of seedlings treated with high dose radiation at 50 or 100 Gy. In this experiment, we developed a sensitive, reliable and rapid method for evaluating genotoxic effect in the nuclei of plant cells by employing comet assay. 방사선에 노출된 고추유묘의 잎으로부터 세포핵을 분리하여 단세포전기영동방법인 comet 분석을 통하여 핵 DNA의 손상정도를 조사하였다. Comet 분석에서 꼬리부분의 길이 (T)와 머리부분의 길이 (H)를 측정하여 T/H 비율을 조사하였다. 무처리세포는 T/H 비율이 1.28이었으나 50 및 100 Gy의 방사선을 처리한 세포는 각각 3.54 및 3.39로 방사선처리에 의해 상당량의 핵 DNA가 손상을 입은 것으로 나타났다. Comet의 head-DNA량은 무처리가 76.8%였으나 50 및 100 Gy를 처리한 세포는 각각 55.9% 및 59.5%를 보였다. 고선량의 방사선을 처리하기 전에 미리 20 Gy 이하의 저선량 방사선을 종자에 전처리하였을 경우 종자의 발아 및 생장에 대한 영향은 없었지만, 후속 고선량에 대한 핵 DNA의 손상은 경감되는 경향을 보였다.

ATM 스위치를 위한 대기행렬의 셀 스케쥴링 방식 제안 및 성능평가

안정희 한국시뮬레이션학회 1999 한국시뮬레이션학회 논문지 Vol.8 No.1

A cell scheduling method of Queue for the ATM switch is proposed and simulated. In this paper, we present the cell scheduling method proper to the proposed queue and the improved queue with Queue Sharing(QS) structure for CBR, VBR, ABR traffic. The proposed QS structure minimizes the CLS(Cell Loss Ratio) of ABR traffic and decreases the CLR of bursty VBR traffic. Also we propose a cell scheduling method using VRR(Variable Round Robin) scheme proper to the high-speed(ATM) switch. The VRR scheme provides a fairness in terms of service chance for the queues in the ATM switch as well as QOS of their cell delay characteristic of CBR and VBR traffic, QOS of ABR CLR. The simulation results show the proposed method achieves excellent CLR and average cell delay performance for the various ATM traffic services in the Queue Sharing structure.

부채마 (Dioscorea nipponica Makino)의 부정근 배양조건과 Steroidal Saponin의 기내생산

안정희,손호용,권순태,손건호 한국식물생명공학회 2005 JOURNAL OF PLANT BIOTECHNOLOGY Vol.32 No.3

Effects of growth regulators on growth of adventitious roots and accumulation of steroidal saponins,such as dioscin, prosapogenin A and prosapogenin C, in cultures of Dioscorea nipponica were determined. The maximum growth of adventitious roots was observed in MS medium supplemented with 30 g/L sucrose and 1.0 mg/L NAA. Addition of BA in combination with NAA appeared to be no effective in the growth of adventitious roots. Among the twenty different adventitious roots formed from different seeds, strain No. 10 was selected based on production ability of dioscin, and its stability through the successive liquid culture. During the first 4 weeks of incubation, contents of steroidal saponins in adventitious roots were negligible but the contents were markedly increased at 5 weeks of incubation. Dioscin and prosapogenin C content in IBA-treated adventitious roots were significantly higher than those in NAA-treated roots. However, content of prosapogenin A was not significantly different among NAA or IBA level. Results provide that liquid culture of adventitious roots of D. nipponica have a potential for mass product ogenin A and prosapogenin C. 액체배양을 통한 부채마의 부정근 배양시 첨가되는 생장조절제의 종류와 농도에 따른 부정근의 생장과 steroidal saponin 생산과의 관계를 조사하였다. NAA와BA 농도별 부정근 생장은 NAA를 단독으로 1.0 mg/L를 처리한 곳에 서 가장 효과적이었으나 NAA에 BA를 첨가하면 부정근의 생장에 오히려 억제효과를 보였다. 20종의 각각 다른 배양주로부터 dioscin, prosapogenin A 및 prosapogenin C의 함 량을 조사한 결과 배양주에 따라큰 차이를 보였는데, 그 중 dioscin 함량이 건물당 2.5%로 가장 높았던 10번 배양주를 선발하였다. 부정근에 함유된 세 종류의 steroidal saponin 함량은 부정근의 생장이 왕성한 4주까지는 낮으나 부정근의 생장이 거의 정지되는 5주째에 급격히 증가하였다. Dioscin과 prosapogenin C의 함량은 NAA보다 IBA를 처리한 곳에서 현저히 높으나 prosapogenin A의 함량은 이들 두성 분의 종류와 농도에 영향을 받지 않았다.

랫드 간경변 모델에서 발현되는 단백질의 탐색

안정희,성진실,오해진,김원우,한광협,백용한 대한간학회 2006 Clinical and Molecular Hepatology(대한간학회지) Vol.12 No.1

딩동, 샘톡 왔어요

안정희,고창훈,이인정 샘터사 2018 월간 샘터 Vol.586 No.-

우리나라 의과대학/의학전문대학원의 인문사회의학 교과목 운영현황 및 학습내용 분석

안정희,권복규,이순남,한재진,정재은 한국의학교육학회 2008 Korean journal of medical education Vol.20 No.2

Purpose: We did this study to find out the current teaching status of the medical humanities and social sciences curriculum in Korean medical schools. Further, we discuss the tasks at hand to improve the curriculum in medical education. Methods: The curricula of 41 medical schools and the syllabi of 10 schools were examined. We analyzed the tables of course organization and contents of integrated medical humanities. After analysis of the contents, they were grouped into 6 categories of medical humanities and social sciences domain. Results: Our results are as follow: 1) there are 3 types of medical humanities and social sciences subject forms: inter-disciplinary(integrated, for example, PDS), multi-disciplinary(separated subject form), and mixed(integrated+separated); 2) most schools offer medical humanities and social sciences in a required class; 3) medical humanities and social sciences are taught through all school years and all 8 graduate medical schools offer a medical humanities and social sciences course from year 1; and 4) the average academic credits for medical humanities are 10 or 11. With respect to the curriculum content, there is some commonality in 10 schools: disease prevention, health improvement, medical ethics, medical regulation, professionalism, and community medicine. Differences were seen in content selection and organization. Conclusion: After brief reviews of the medical humanities and social science curriculums, we discovered that all Korean medical schools meet the need of medical humanities and social sciences education. However, curriculum implementation differed in various ways. We suggest the following tasks: 1) clarification of educational goals in order to develop a core curriculum of medical humanities and social sciences in Korea; 2) sharing experiences of developing a well-designed curriculum with other medical schools for effective teaching of this subject area.

운동생리 분야 : 수영훈련의 생리적 효과 분석 - 대구시내 여중 , 고 선수를 중심으로 -

안정희,장인현 한국체육학회 1991 국제스포츠 학술대회 Vol.1991 No.-

일개 의학전문대학원 교육과정 평가 결과 분석

안정희,한재진,김나진,어은경,권복규,이순남 한국의학교육학회 2010 Korean journal of medical education Vol.22 No.1

Purpose: This study aims at to make intermediate-term evaluations of a curriculum by investigating its development, operation and outcomes 2 years after its revision. Methods: A survey using 5-point-Lickert scale questionnaires was given to the group of directors who developed the curriculum,instructors who only used it in their classes, and a student group from the first and second grades. Focus group interviews were performed in the professor groups. Results: Curricular reform was evaluated as being systematic, democratic, and positive in general. Both groups answered questions positively about the relevance of the integrated curriculum and introduction of clinical medicine (ICM), graded as 3.4 (professor)and 3.5 (student). As for problem-based learning (PBL) and the patient-doctor-society (PDS), the professor group responded more positively than students. The 'web-based learning center' was recognized positively by many more students (4.01) than professors (2.75). With regard to the education outcome, professors gave 3.3 points and students 3.5 to an item that asked 'whether students attained the learning goal or not?' Professors, through interviews, showed their satisfaction with the attempt to reform the curriculum,but they pointed out that long-term evaluations should be performed. Conclusion: The interim evaluation of the revised curriculum, from its planning to its effects, affirmed by several suggestions to be successful in the long run through 1) enhancement of systematic participation and communication, 2) further integration, 3) steady evaluation, 4) greater effort on professional development, and 5) active interaction between professors and students.

지방분권 시대의 청년공동체를 통한 지역공동체 활성화 방안

안정희 한국자치발전연구원 2017 자치발전 Vol.2017 No.8