Comet 분석을 통한 방사선처리 고추세포의 핵 DNA 손상평가

안정희,백명화,김재성,정정학,권순태,An, Jung-Hee,Back, Myung-Hwa,Kim, Jae-Sung,Jeong, Jeong-Hag,Kwon, Soon-Tae 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3

We employed single cell gel electrophoresis method (comet assay) to analyze the degree of nucleus-DNA damage in the leaves of red pepper (Capsicum annuum L.) seedlings exposed to $^{60}$ CO v-radiation stress. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length (H) in individual comet image isolated from pepper leaf cell. The T/H ratio of control-cells and treated-cells at 50 or 100 Gy were 1.28 and 3.54 or 3.39, respectively, suggesting that nuclei of pepper cells were severely damaged in the integrity of DNA strand by the treatment of enhanced v-radiation. The percentage of head-DNA in control-cells was 76.8%, whereas those of 50 and 100 Gy treated-cells were 55.9% and 59.9%, respectively. Pretreatment of low dose (4 to 20 Gy) radiation to seeds decreased DNA-damage in the leaves of seedlings treated with high dose radiation at 50 or 100 Gy. In this experiment, we developed a sensitive, reliable and rapid method for evaluating genotoxic effect in the nuclei of plant cells by employing comet assay. 방사선에 노출된 고추유묘의 잎으로부터 세포핵을 분리하여 단세포전기영동방법인 comet 분석을 통하여 핵 DNA의 손상정도를 조사하였다. Comet 분석에서 꼬리부분의 길이 (T)와 머리부분의 길이 (H)를 측정하여 T/H 비율을 조사하였다. 무처리세포는 T/H 비율이 1.28이었으나 50 및 100 Gy의 방사선을 처리한 세포는 각각 3.54 및 3.39로 방사선처리에 의해 상당량의 핵 DNA가 손상을 입은 것으로 나타났다. Comet의 head-DNA량은 무처리가 76.8%였으나 50 및 100 Gy를 처리한 세포는 각각 55.9% 및 59.5%를 보였다. 고선량의 방사선을 처리하기 전에 미리 20 Gy 이하의 저선량 방사선을 종자에 전처리하였을 경우 종자의 발아 및 생장에 대한 영향은 없었지만, 후속 고선량에 대한 핵 DNA의 손상은 경감되는 경향을 보였다.

방사선과 자외선에 대한 고추 식물체 및 배양세포의 생장반응과 Capsidiol 생합성 유전자의 발현 차이

안정희,김재성,정정학,오세명,권순태,An, Jung-Hee,Kim, Jae-Sung,Jeong, Jeong-Hag,Oh, Sei-Myoung,Kwon, Soon-Tae 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2

Differential responses of red pepper plant and cultured cells to enhanced ${\gamma}$-ray($^{60}$ Co) and ultraviolet(UV) stress were investigated. In seed treatment, 1 Gy of ${\gamma}$-ray increased seedling dry weight up to 19.1%, but 50 Gy treatment markedly ingibited seed germination and subsequent growth of seedling. UV treatment to seed did not change the germination ability of seeds and the growth of seedlings regardless of duration of UV treatment until 24 hrs. In case of UV treatment to seedlings, plant injury was seriously progressed even after the seedlings were returned to no UV condition, and eventually all the leaves showed chlorosis by the stress. However, progress of plant injury by ${\gamma}$-ray stress slower than that caused by UV stress, and even at the high dose of ${\gamma}$-ray 50 Gy, did not caused the cholrosis of stressed plant leaf. Amount of electrolytes leakage from plant leaf by UV treatment for 24hrs was increased up to 28.8 folds in comparison with untreated control, whereas that of 50 Gy of ${\gamma}$-ray was increased only 1.2 folds. UV stress induced the production of capsidiol, antimicrobial phytoalexin, by activation of gene expression involved in capsidiol biosynthesis, such as sesquiterpene cyclase and cyclase and cytochrome P450 hydroxylase in the leaf and cultured cell, but ${\gamma}$-ray stress induced neither the production of capsidiol nor expression of the genes.

친환경 식품과 가공 식품에 대한 주부들의 인식 및 소비 형태

안정희 ( Jeong Hee An ),강근옥 ( Kun Og Kang ) 한국식품영양학회 2006 韓國食品營養學會誌 Vol.19 No.1

均如鄕歌에 사용된 否定의 표현에 대하여

안정희(Ahn, Jeong-hee) 구결학회 2011 구결연구 Vol.27 No.-

본고는 균여(923-973)의 普賢十願歌를 대상으로 借字를 사용하여 표현한 否定의 체계를 밝힌 것이다. 여기에 나타난 否定의 표현은 중세국어나 현대국어의 그것과 통사적, 의미적 기능에 있어 큰 차이가 없는 것으로 밝혀졌다. 중세/현대국어의 '아니 부정문'과 '몯 부정문'은 각각 借字덩어리 不冬/不?와 毛冬/毛等으로 표현되었다. '不冬'은 모두가 용언부정문에 사용되었으며 '不?'는 체언부정문에 사용되었다. 毛冬/毛等은 용언부정문에 사용되었다. '毛冬留'는 중세어 존재사 '잇다'의 반의어인 '없다'의 부사적 활용형으로 기능한다. '毛冬留'의 분포상황과 통사적 기능을 분석하여 지금까지 '間王'으로 오독되어온 차자 '王'자가 사실은 부정소를 형성하는 '毛'라는 것을 발견하였다. 그리하여 '間王 + 冬留'로 끊어 읽던 것을 '間 + 毛冬留'로 끊어 읽어야 된다는 사실을 밝혔다. '없다'는 의미를 가진 '毛冬留' 외에도 '于萬/于音' 그리고 '无叱/无史' 등이 함께 씌었다. This paper aims to find out which Chinese characters or cluster of characters have been used for negation in the Kyunyo-Hyangga, and to describe their syntactic and morphological features. The characters and the cluster of characters containing negational morphemes are analyzed in their distribution, combination and syntactical circumstances, in which they occur. For this purpose, the concordance of the original text, in which the characters are presented in sentences, i.e. in lines of a song, were used. In the Kyunyo-Hyangga, like in Middle and in Modern Korean, two kinds of negation could be found, ani-puchongmun and mot-puchongmun. The cluster 不冬/不? are used for ani-puchongmun, and 毛冬/毛等 for mot-puchongmun. 不冬 always preceedes the verb to be negated, while 不? occurs between nouns. 毛冬/毛等 precedes a verb, which is to be negated. The analysis of frequency and combinability of a given character or clusters of characters, i.e. the surrounding characters, lead me to correct a hitherto wrong-interpreted character 王 to 毛. The correction could be verified by morphological, syntactical and semantical analysis as well as by the comparison of the graphical presentation. The cluster of characters 毛冬留 meaning 'have not' is an adverbial flexional form of the MKor. verb 'ops-', which is an antinomy of the existentialia 'is-'. The syntatic analyses of the sequence of the characters 毛冬 ana 毛冬留 demonstrate that, semantically they belong to a different paradigm of negation, in which the former means 'can not' and the latter 'have not'. Further, for the negation meaning 'have not1, 于萬/干音 and 无叱/无史 are used parallel in the text of the Kyunyo-Hyangga.

ATM 트래픽을 위한 가변큐공유(VQS) 메카니즘

안정희(Ahn Jeong Hee),정진욱(Chung Jin Wook) 한국정보처리학회 1999 정보처리학회논문지 Vol.6 No.3

This paper proposes the mechanism of cell buffering in the output buffer of ATM switch for the traffic with the different QoS in the ATM environment. The proposed mechanism. VQS(Variable Queue Sharing) can minimize the cell loss ratio(CLR) of bursty traffic through the sharing of CBR queue, VBR queue, ABR queue to maximize the utilization of queue resource. To evaluate VQS performance, we make simulator using Visual Slam 2.0. We compare the CLP and cell average delay of VQS and HOL., QLT_HOL using the bursty traffic patterns.

항암화학요법의 구토유발 수준별 예방적 항구토제 프로토콜의 이행정도

최자윤(Choi Ja Yun),오현정(Oh Hyeon Jeong),강지영(Kang Ji Young),김민경(Kim Min Kyoung),김지은(Kim Ji Eun),김진하(Kim Jin Ha),김희숙(Kim Hee Suk),박소라(Park So Ra),변정선(Byun Jeong Seon),안정희(An Jeong Hee),조민경(Cho Min Kyoung) 대한종양간호학회 2011 Asian Oncology Nursing Vol.11 No.1

Purpose: The purpose of this study was to identify the compliance with the protocol, which was developed considering the emetogenic potential for prophylaxis of chemotherapy. Methods: Data was collected from 144 patients who received chemotherapy from June 15 to August 31, 2010 in C University Hospital in Jeollanamdo, Korea. The level of chemotherapy-induced nausea and vomiting (CINV) and the compliance with the protocol for prophylaxis of CINV were measured. Results: There was statistically significant difference of CINV in morning sickness and anticipatory nausea of general and clinical characteristics. Also, the compliance with the protocol developed according to emetogenic potential of chemotherapy was statistically significant. There was no difference in CINV in regard to the compliance with the protocol. Conclusion: There was a good compliance with the protocol for prophylaxis according to emetogenic potential. But it should be recommended to use antiemetics for prophylaxis aggressively to relieve CINV for the patients who already experienced morning sickness and anticipatory nausea. In addition, the oncology nurses should respond sensitively to the complaints of nausea and vomiting no matter what the emetogenic potentials of chemotherapy regimen are.

원 간섭기 고려왕의 대원정책과 복식정책의 관계연구

전혜숙(Chun Hea-Sook),안정희(Ahn Jeong-Hee) 한복문화학회 2006 韓服文化 Vol.9 No.2

The main factor greatly affecting changes in costume is political relations under which the ruler and the ruled interactively change and influence each other. In this respect, it is unreasonable to ignore Koryo's political relations with Win in discussing the costume of the former nation at a time when Win was politically ruling Koryo. Profound approaches to the costume of the later Koryo period when the nation was politically subject to Win are essential to determine the identity of the costume of the early Chosun period because the costume of Chosun succeeded that of Koryo. Thus the purpose of this study is to how the blood relationships and social statuses of Koryo's kings and their queens were reflected in the nation's perceptions and policies towards Win and further related with the costume policy of Koryo. For the purpose, this researcher reviewed literatures like 〈History of Koryo〉 and 〈Koryosa Jeolyo〉 both of which contain records about costume policies taken for 115 years from the 10th year of king Wonjong(1259) to the 23rd year of king Gongmin(1374) by five monarchs of Koryo who married princesses of Win. Results of the study can be summarized as follows. Families of Win's princesses who became queens of Koryo's kings in accordance with marriage relationships between the royal courts of the two nations exerted politically great influences in Koryo, on which the survival of the throne, warranty of royal authority and diplomatic relations with Win were dependent. In Koryo, some kings who took positive pro-Win policies also positively accepted the costume of Win while, others who took anti-Win policies tried to restore the costume of Koryo. As Win declined and Ming rose, kings of Koryo accepted the costume of Ming as a part of their pro-Ming policies. This suggests that there were indispensible relations between changes in international situations and costume policy. In Koryo, thus, costume policy was a very sophisticate political means by which internal and external political relations were represented.

해쉬체인을 이용한 인증서 상태 검증 방법들의 문제점과 해결 방법

강현중(Kang Hyun Joong),안정희(Ahn Jeong Hee) 한국컴퓨터정보학회 2008 韓國컴퓨터情報學會論文誌 Vol.13 No.1

해쉬체인을 이용한 사용자의 인증과 무결성 제공방법이 활성화되면서 이를 이용한 인증서 상태검증 방법들이 다양하 게 제안되었다. 제안된 방법들중에 NOVOMODO에서는 CA가 해쉬값을 생성하여 각 사용자에게 배포하고 JianyingZhou의 프레임워크와 양종필의 개선된 프레임워크에서는 사용자가 해쉬값을 생성하여 검증자에게 전달한다. 그러므로 해쉬값의 생성 및 배포를 위한 작업량이 각 사용자에게 분산되어진다. 그러나 이들 프레임워크는 CA가 발행한 인증서에 오류가 없고 CA의 개인키가 노출되지 않는다는 가정을 전제로 하고 있다. CA도 공격자에 의해 언제든지 개인키가 노출될 수 있으므로 이들 프레임워크는 실제 PKI 환경에 적합하지 않다. 따라서 본 논문에서는 사용자의 인증서에 CA가 제어할 수 있는 해쉬값을 추가하여 이를 해결하고자 한다. 추가되는 해쉬값은 CA내의 사용자들에게 동일하며 CA에서 해쉬값을 생성, 저장 및 배포하기 위한 비용이 적다. 그리고 우리는 또한 양종필의 프레임 워크에서 서명 및 검증 절차의 문제점을 지적하고 개선 방법을 제안한다. 우리의 제안은 다른 프레임워크들보다 실제 PKI 환경에 적합하다. As the authentication and the integrity methods based on the hash chain are popular, several certificate status validation methods based on the same function are proposed at the moment. In NOVOMODO, a CA generates and releases the hash value to each user. In Jianying Zhou’s framework and Jong-Phil Yang’s framework, a user generates and releases the hash value to verifier. Therefore, the CA loads are distributed to each user. However, these frameworks are based on the assumption that the CA’s secret key is not lost or compromised and the certificates issued by the CA are error-free. Therefore, these frameworks are not suitable in real PKI environments. In this paper, as one hash value generated by CA is included in the user’s certificate in addition, the certificate revocation published by CA using that value can be managed. The hash value included in user’s certificate is the same for all users. The computation costs, the storage amounts and the release costs are small in the CA. And we modify the procedure for the signature and its validation in Jong-Phil Yang’s framework. Our solution is more suitable than those frameworks in real PKI environments.

공기공급량이 음식폐기물의 퇴비화 반응에 미치는 영향

정준오(Joon Oh Jung),안정희(Jeong Hee An) 大韓環境工學會 2001 대한환경공학회지 Vol.23 No.6

HFC-CATV 망에서 멀티미디어 QoS 제공을 위한 MAC 프로토콜

이수연(Su-Youn Lee),안정희(Jeong-hee Ahn) 한국멀티미디어학회 2002 한국멀티미디어학회 학술발표논문집 Vol.2002 No.2