개정된 도로교통법 수행을 위한 자율주행 자동차 알고리즘 개발

김유중(Yu-Jung Kim),조경근(Kyoung-Geun Cho),조훈희(Hun-Hee Cho),이준우(Jun-Woo Lee),이유빈(Yu-Bin Lee),제프리 자흐라 바툴(Zahra-Batool Jaffrey),강준우(Jun-Woo Kang),이종혁(Jong-Hyeok Lee),강태구(Tae-Koo Kang) 대한전기학회 2022 대한전기학회 학술대회 논문집 Vol.2022 No.11

Establishment of a canine model of human type 2 diabetes mellitus by overexpressing phosphoenolypyruvate carboxykinase

JEONG, YEON WOO,LEE, GEUN-SHIK,KIM, JOUNG JOO,PARK, SUN WOO,KO, KYEONG HEE,KANG, MINA,KIM, YU KYUNG,JUNG, EUI-MAN,HYUN, SANG HWAN,SHIN, TAEYOUNG,JEUNG, EUI-BAE,HWANG, WOO SUK Spandidos Publications 2012 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.30 No.2

<P>Dogs are useful models for studying human metabolic diseases such as type 2 diabetes mellitus due to similarities in physiology, anatomy and life styles with humans. Somatic cell nuclear transfer (SCNT) facilitates the production of transgenic dogs. In this study, we generated transgenic dogs expressing the phosphoenolpyruvate carboxykinase (PEPCK) gene, which is closely involved in the pathogenesis of type 2 diabetes mellitus. In addition, we assessed the cloning efficiency associated with adult or fetal (cloned or natural mating) fibroblasts as a nuclear source. Cloning efficiency was determined by the fusion, pregnancy and cloning rates. The fusion rates were significantly high for fibroblasts from cloned fetuses, but the pregnancy and cloning rates were relatively high for cells from normal fetuses. Based on these data, fetal fibroblasts were selected as the nuclear donor for SCNT and genetically engineered to overexpress the PEPCK gene and dual selection marker genes controlled by the PEPCK promoter. The transgenic cells were introduced into oocytes and transferred into five recipient dogs, resulting in two pregnancies. Finally, three puppies were born and confirmed by microsatellite analysis to be genetically identical to the donor. One puppy successfully overexpressed PEPCK mRNA and protein in the liver. This canine disease model may be useful for studying the pathogenesis and/or therapeutic targets of type 2 diabetes mellitus.</P>

DWP305 ( Ursodeoxycholic acid Silymarin Fursulthiamine Riboflavin tetrabutyrate ) 의 경구 투여에 의한 랫드에서의 급성 및 아급성 독성 연구

남석우(Suk Woo Nam),박승희(Seung Hee Park),유세근(Se Geun Yu),서동완(Dong Wan Seo),김형식(Hyung Sik Kim),이병무(Byung Mu Lee),심점순(Jeon Soon Shim),유영효(Young Hyo Yu),박명환(Myung Hwan Park),이향우(Hyang Woo Lee) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.3

The acute toxicity of DWP305 (Ursodeoxycholic acid : Silymarin : Fursulthiamine : Riboflavin tetrabutyrate=1 : 1 : 0.1 : 0.05) was evaluated in both sexes of Sprague-Dawley rats, 6 weeks old by the oral route of administration. DWP305 was not considered to induce any toxic effect on the rats in mortalities, clinical findings, body weights and gross findings. It is suggested that LD_(50) value in rats would be above 5 g/㎏ in the oral administration. Subacute toxicity of DWP305 was examined in Sprague-Dawley rats. Four groups of rats were administered orally at doses of 0, 0.32, 0.8, and 2.0 g/㎏/day of DWP305 for one month. Any significant toxic clinical symptom was not observed in the treated rats during the experimental period. Macroscopic examination on the organs of tested animals showed no abnormal findings. On autopsy, no significant changes were found in organs examined. Maximum tolerated dose of DWP305 for the rat was estimated to be above 2 g/㎏ in this study.

Blockade of central vascular endothelial growth factor-A pathway attenuates inflammatory orofacial pain in rats

( Geun-woo Lee ),( Jo-young Son ),( Yu-mi Kim ),( Min-jeong Jo ),( Dong-kuk Ahn ) 조선대학교 치의학연구원 2023 Oral Biology Research (Oral Biol Res) Vol.47 No.4

The present study investigated the role of the vascular endothelial growth factor (VEGF)-A pathway in inflammatory pain transmission in the orofacial region of rats. Complete Freund’s adjuvant (CFA; 40 μL) or formalin (5%; 50 μL) was administered subcutaneously in the vibrissa pad of male Sprague-Dawley rats (weight: 230-280 g) to induce inflammatory pain. The findings showed that subcutaneous injection of CFA increased thermal hypersensitivity 1 day after injection, while administration of formalin produced a biphasic nociceptive behavioral response. Furthermore, subcutaneous pretreatment with VEGF-A164 antibody did not affect head withdrawal latency time in either treatment group. In contrast, intracisternal injection of VEGF-A164 antibody (1, 5 μg) significantly suppressed thermal hypersensitivity in the CFA group and the number of scratches observed in the second phase in the formalin group. Furthermore, intracisternal administration of VEGF-A type 1 (100 μg) and VEGF-A type 2 (20 μg) receptor inhibitors resulted in attenuation of the number of scratches observed in the second phase in the formalin group. These results suggest that blockade of the central VEGF-A pathway can serve as a potential therapeutic approach for the onset of inflammatory pain in the orofacial region.

Carbachol에 의한 닭 췌조직내 nitric oxide synthase 활성 및 methylated arginine 함량변화

유세근,남석우,박승희,남태균,서동완,한정환,홍성렬,이향우 성균관대학교 약학연구소 1994 成均藥硏論文集 Vol.6 No.1

The role of nitric oxide (NO) in the regulation of exocrine secretion was investigated in chicken pancreatic tissues. Nitric oxide synthase (NOS) in the pancreatic tissues were Ca^2+-dependent form. Treatment of tissue with L-arginine (10 mM) or carbachol (10^-5 M) showed the increase of pancreatic exocrine secretion, but that of D-arginine (10 mM) did not. ^3H-citrulline formation, cyclic GMP level and methyl arginine contents in the homogenates of pancreatic tissues treated with carbachol were not affected. Although the action mechanism of NO in the pancreatic tissues remains unknown, NO apparently participates significantly in the overall control of pancreatic secretory function.

재발성 가와사끼병의 임상양상과 검사소견

유승택,최은정,이창우,오연균,박승택,윤향석 圓光大學校 醫科學硏究所 2003 圓光醫科學 Vol.18 No.2

ERP, AT 실무교육이 취업 특성에 미치는 영향

조유근(Yu-Geun Jo),조승제(Seung-Je Jo),신춘우(Chun-Woo Shin) 조선대학교 지식경영연구원 2020 기업과 혁신연구 Vol.43 No.1

본 연구에서는 ERP, AT 실무교육이 취업특성에 미치는 영향을 검증하기 위하여 Pearson’s correlation coefficient를 이용한 상관관계 분석과 평균차이 분석, 이분형 로지스틱 회귀분석을 실시하였으며, 유의수준 p<0.1( 90%)에서 해석하였다. 본 연구의 구성은 다음과 같다. 제Ⅰ장에서는 연구의 배경 및 연구의 목적, 연구의 범위 및 방법을 기술하였고, 제Ⅱ장에서는 본 연구와 관련된 선행연구와 가설설정을 서술하였으며, 제Ⅲ장 연구설계에서는 연구모형과 변수정의에 대하여 기술하였다. 그리고 제Ⅳ장 실증분석에서는 기술통계 결과를 제시하고 상관관계분석의 결과 및 해석과 평균차이분석의 결과 및 해석, 이분형 로지스틱 회귀분석의 결과 및 해석을 기술하였으며, 마지막 제Ⅴ장에서는 연구의 결과와 시사점을 기술하였다. 본 연구모형의 이분형 로지스틱 회귀분석 결과 및 해석을 요약하면 다음과 같다. 첫째, 연구모형 1-1의 이분형 로지스틱 회귀분석 결과에서는 ERP 인사정보관리사 자격취득이 취업시기에 유의한 영향력을 미치는 것으로 나타남으로써 연구가설이 채택되었다. 그리고 연구모형 1-2의 이분형 로지스틱 회귀분석 결과에서도 ERP Master 자격취득, ERP물류 정보관리사 자격취득, ERP생산 정보관리사 자격취득이 취업기업 규모에 유의한 영향력을 미치는 것으로 나타남으로써 연구가설이 채택되었다. 또한 연구모형 1-3의 이분형 로지스틱 회귀분석 결과에서는 ERP물류 정보관리사 자격취득, ERP생산 정보관리사 자격취득이 취업지역에 유의한 영향력을 미치는 것으로 나타남으로써 연구가설이 채택되었다. 둘째, 연구모형 2-3의 이분형 로지스틱 회귀분석 결과에서는 TAT 2급 자격취득이 취업지역에 유의한 영향력을 미치는 것으로 나타남으로써 연구가설이 채택되었다. 셋째, 연구모형 3-2의 이분형 로지스틱 회귀분석 결과에서는 본사방문 여부와 임원특강 참여여부가 취업기업 규모에 유의한 영향력을 미치는 것으로 나타남으로써 연구가설이 채택되었다. Correlation analysis using pearson’s correlation coefficient, mean difference analysis and binary logistic regression analysis were used to analyze the effects of employment characteristics of accounting practical education, such as ERP and AT using significance level p<0.1 (90%). For this study, we made following contents. Chapter 1 describes research background, research purpose, research range and research method. Chapter 2 describes precedent researches literatures related and research hypothesis, Chapter 3 which is research design describes the research models and definitions of variables. Chapter 4 which is empirical analysis provides the results of descriptive stastics and describes the results and explanations on the correlationa analysis, mean difference analysis and binary logistic regression analysis. And chapter 5 describes the implications and results of the research. This analysis registered samples at A university in Gwangju. Compositions of this study are following. The first chapter described backgrounds of study, objects of study and methods of study. The second chapter described advanced research and hypothesis. So, the third chapter described research model and defined variable. And the fourth chapter analyzed and interpreted the correlation analysis and the multiple regression analysis. Finally, the fifth chapter suggested conclusion of the study, limitation of the study and future direction of the study. Following is the summary of the results and explanations of binary logistic regression analysis of this research model. First, the result of binary logistic regression analysis of research model 1-1 shows that the acquisition of license of ERP (personnel information manager model) made significant effects to the employment period accepting the research hypothesis. Second, the result of binary logistic regression analysis of research model 2-3 shows that the acquisition of license of TAT 2 made significant effects to the employment locations accepting the5 research hypothesis. Third, the result of binary logistic regression analysis of research model 3-2 shows that the visiting headquarter and participation on the directors’ special speech made significant effects to the size of employment company accepting the research hypothesis.

Streptococcus sp. 기원의 Protease를 이용한 Casein Phosphopeptides의 생산

임근형,이병우,박기문,손세형,유주현 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.5

칼슘의 불용성화를 저해하는 casein phosphopeptides(CPP)를 제조하기 위하여 sodium caseinate를 기질로 하여 기존의 trypsin과는 다른 Streptococcus sp.로부터 생성된 조효소를 사용하여 최적생성조건, 생산수율 및 그 특징을 검토하였다. 최적조건은 기질농도 10%에서 조효소 사용량 1.5%, 반응온도 50℃, pH 8.0 및 반응시간 4시간으로서, 이때의 CPP 생성율은 23%였다. 또한, CPP의 분자량은 3,000∼17,000으로 확인되었으며, in vitro에서 칼슘양에 대하여 1.5배의 사용으로 칼슘의 불용성화를 방지할 수 있었다. For the production of Casein Phosphopeptides(CPP) inhibiting the insolubility of calcium, 10% sodium caseinate was treated with 1.5% of protease from Streptococcus sp.. Optimal conditions and productivity for the CPP production, and properties of the CPP were compared with tryptic hydrolysates of sodium caseinate. Optimum conditions of pH, temperature and reaction time were 8.0, 50℃, 4 hrs, respectively. Under these conditions the productivity of CPP was 23% and Molecular weight of CPP was ranged from 3,000 to 17,000. The results also showed that the insolubility of calcium was completely inhibited by using 1.5 times of CPP for the amount of calcium.

사용자의 다중모드 단말기를 위한 무선서비스 최적 선택방법에 대한 연구

한우휘(Yu-Hui Han),조근호(Geun-Ho Cho),최진우(Jin-Woo Choe),전성익(Sung-Ik Jun),김영세(Young-Sae Kim) 한국통신학회 2005 한국통신학회 학술대회 및 강연회 Vol.2005 No.4

유한요소해석을 이용한 유도상 아스팔트 궤도의 피로균열 설계수명 예측

송근우(Geun Woo Song),박재범(Jae Beom Park),임유진(Yu Jin Lim),이진욱(Jin Wook Lee) 한국철도학회 2014 한국철도학회 학술발표대회논문집 Vol.2014 No.5

본 연구에서는 유도상 아스팔트 궤도 노반층의 손상도에 입각한 설계수명 산정을 위해 손상기준을 아스팔트 노반의 피로파괴로 국한하였다. 아스팔트 노반을 포함한 궤도에 대해 목표설계수명을 50년으로 설정하고 궤도구조형식별, 궤도구조구성 조건별로 유한요소해석 프로그램인 ABAQUS를 이용한 구조해석을 실시하였다. 해석결과와 손상도이론을 이용하여 예상 설계수명의 만족여부를 판정하였다. 구조해석으로부터 산정된 아스팔트 노반층 하면 인장변형률의 크기와 피로파괴 기준식을 이용하여 아스팔트 노반의 설계수명을 예측하였으며, 강화노반 탄성계수(ESB)가 유도상 아스팔트 궤도의 설계수명에 가장 큰 영향을 미치는 것으로 나타났다. 특히 년간통과톤수가 클 경우에는 목표 설계수명을 확보하기 위해 일정 값 이상의 강화노반 두께와 강화노반 탄성계수의 확보가 반드시 필요한 것으로 검토되었다. In order to calculate design life of ballasted asphalt trackbed, damage is limited to fatigue crack only. A structural analysis was performed using ABAQUS program. In this study, primary design parameters such as types of track sections, wheel load size, velocity, MGT, elastic modulus and thickness of each layers are defined. Full 3D Finite element analyses were performed using ABAQUS program in order to calculate tensile strains under asphalt layer with different values of design parameters defined. The target design life is checked by using damage theory and computed results. It was verified that modulus of subbase is the most critical onto the design life of the ballasted asphalt trackbed. It was concluded that it is required to keep minimum thickness and minimum modulus of reinforced roadbed should be used in case of large amount of traffic(MGT)